The concentrations of lactoferrin (LF) in colostrum during the first 8 days and mature milk during the first 4 months of postpartum were determined by electroimmunodiffusion. Meanwile a sensitive enzyme linked imm-unosorbent assay for measurements of LF in neonatal faeces were established. The results showed that human milk, especially colostrum, was rich in LF. The colostrum contained 6.88 ? 3.10mg/ml (M?SD) aad mature milk 3.18 ? 0.37mg/ml LF. LF in human milk was the highest during the first 3 days of lactation with a decline in concentration as lactation proceeds although a wide variation in concentration was noted among different individuals.LF in neonatal faeces showed significant difference in different feeding manners. LF level in the breast-fed infants was 19 times higher than that in the bottle-fed infants. The LF percentage detected in the former was 100% and the latter only 60%. The dynamic variations of LF in faeces of breast-fed infants during the first day of life were found to be relative to LF level in mother'milk.These data demonstrated the influence of high LF level in mother'milk on the concentrations of LF in breast-fed infants' intestine.Furthermore a few amounts of LF was found in meconium and its percentage detected was 35%. This suggested that the production of endogenous LF began from foetus period, but was limited during the first day of life. LF ingested by the breast-fed infants were not completely destroyed and thus may supplement the insufficiency of the endogenous LF in the newborn infants' intestinal tract.

OBJECTIVE:To develop a RAM(Restricted-access media)-HPLC for the determination of plasma concentration of carbamazepine,and to compare the determination results with those determined by FPIA method. METHODS: The plasma samples were centrifugalized,then the venous plasma samples were determined by FPIA and RAM-HPLC,respectively,while the fingertip plasma sample was determined by RAM-HPLC alone. The chromatographic column was Hisep shielded hydrophobic phase analytical column,and the mobile phase consisted of ammonium acetate buffer-acetonitrile (88∶12) at a flow rate of 1.5 mL?min-1. The detective wavelength was set at 280 nm;the sample size was 20 ?L and the column temperature was kept at room temperature. RESULTS: A good correlation was noted between the carbamazepine concentration in venous plasma sample determined by FPIA and carbamazepine concentrations in either venous or fingertip plasma determined by RAM-HPLC,with the correlation coefficients being 0.989 and 0.995,respectively,but the differences were significant(P0.05). CONCLUSION: Both RAM-HPLC and FPIA are applicable for the determination of carbamazepine concentration,but FPIA is more suitable for routine monitoring while RAM-HPLC is more suitable for the related research and monitoring of specific cases. Both methods shows good reproducibility and in which the samples don't have to be pretreated.

Adult ; Death, Sudden ; Humans ; Male ; Middle Aged ; Trichloroethylene ; poisoning

Acupuncture Points ; Acupuncture Therapy ; Adult ; Aged ; Female ; Humans ; Male ; Middle Aged ; Sciatica ; therapy

Due accomplishments in the research of medical humanities and social science in higher medical college was failed to achieve due to the problems of improper intellectual structure of the researchers,prejudices toward this discipline,non-mainstream,non-profession and poor research environment.This article brings forward three proposals which may improve the research of medical humanities and social science,namly the establishment of independent discipline,the system of training and practice for researchers and the construction of problem-centered interdisciplinary research platform.

In recent years , along with the continuous accumulation of clinical experiences and the improvement of surgical devices, the percutaneous coronary intervention (PCI) has become a perfect treatment method and been widely used in the clinic . Hence , the coronary heart disease ( CHD ) patients have received better treat-ment results. However, the in-stent restenosis is still a big problem after PCI. The combination of Chinese and modern medicine in the prevention of restenosis has become a focused hotspot . In order to further under-stand this issue , this article discussed the Chinese and modern medicine mechanism in order to provide more thoughts for clinical practice .

Magnetic resonance imaging(MRI) has multi-faceted advantages due to its non-ionization radiation, excellent tissue contrast, high spatial resolution and multi-dimensional imaging technique. It can clearly observe the pathological changes of deseases, and judge for the prognosis. With the development of MRI, MRI has become increasingly popular in the diagnosis of fetal malformation. In this paper, it is described the value of MRd in the diagnosis, management and prognosis of fetuses malformations, via the central nervous system, circulatory system, respiratory system, digestive system, genitourinary system, musculoskeletal system, endocrine system, and so on.

Objective To discuss the activity of Toxoplasma gondii and its impact on fetus during pregnancy.Methods Sixty -eight pregnant women whose IgG was positive and IgM was negative for anti - Toxoplasma gondii antibodies were chosen.Their serum and umbilical blood were tested for IgG,IgM,CAg (by ELISA) and DNA (by PCR) of Toxoplasma gondii.The infections of placental samples were tested by the direct smear,homogenate smear and PCR.Results There were 28 positive cases of Toxoplasma gondii IgG antibodies in cord blood,and the vertical transmission rate of IgG in placenta was 41.2%.There were 6 positive cases of Toxoplasma gondii DNA in cord blood,and the incidence of intrauterine infection was 8.8%.There were 9 positive cases of Toxoplasma gondii DNA in placental tissues,and the incidence of intrauterine infection was 13.2%.Conclusions Past Toxoplasma gondii infection during pregnancy may lead to vertical transmission between the mother and infant.