Objective To optimize and verify an in vitro detection method for bioactivity of recombinant human bone morphogenetic protein-2(rhBMP-2)based on ASTM F2131-02 standard test.Methods Based on the method established by the American Society for Testing Material(ASTM),the detection cell lines(W-20-17 and C2C12 cells),dilution mode of rhBMP-2(2 and 3 times serial dilution),culture time(24,36,48 and 72 h),and freezing temperature(-20 and-80 ℃)were optimized,and the specificity,intermediate precision,linearity and durability of the optimized method were verified. The specific activity of rhBMP-2 from different manufacturers was detected using the optimized method.Results C2C12 cells were determined as detection cells;The best dilution method of rhBMP-2 was 2 times series dilution;The optimum cell culture timewas 48handtheoptimum freezingtemperatureofcellswas-80℃. C2C12 cells responded specifically to rhBMP-2 induction when adding heteroprotein(bovine serum albumin)to rhBMP-2;The CV of 12 detections by 2 experimenters at 2 time points was11. 7%. The theoretical titer of rhBMP-2 showed a good linear relationship with the measured titer in the range of 70%-142%,and the fitting linear regression equation was:y = 0. 983 1 x-0. 010 3,R~2= 0. 996 4;The CV of the detected specific of rhBMP-2 using 4,11 and 21 passages of C2C12 cells was 5. 2%. The CV of specific activity of rhBMP-2 samples from different manufacturers detected by the optimized method repeatedly for two times was 1. 3%-7. 5%.Conclusion The optimized method showed good specificity,intermediate precision,linearity and durability,and might be used to detect the bioactivity of rhBMP-2 in vitro,which provided a reliable analytical method for its research,production and application.

The first step of bacilli culture is inoculation bacteria on culture medium. Designing a device to increase efficiency of inoculation is significative. The new device is controlled by SCM. The stepper motor can drive the culture medium rotating, accelerating, decelerating, overturn and suspending. The device is high practicability and efficient, let inoculation easy for operator.
Bacteriological Techniques ; instrumentation ; Equipment Design ; Software Design

[Objective] Have a research on therapeutic methods of phlegm syndrome of Li Ziyi in Qing dynasty, to sum up his therapeutic experience of such syndrome.[Methods]Analysis on the related states and cases on the base of researches on the contents of “Approaches of phlegmatic scrofula ”which was written by Mr. Li, elaborating his characteristics in treating such syndrome. [Results] Mr. Li considered that phlegm syndrome can be classified into milder phlegm and scrofula according to its level of the patient ’s condition and therapeutic effect. He also believed that several aspects should be taken into consideration through the therapeutic process:treat by internal combing with external treatment ,pay attention to seven emotions and smoothen the temper, emphasize nursing care and obey to the contraindication strictly. [Conclusion]There are features in Mr. Li ’s view on phlegm and his treating method of phlegm syndrome. His treating method has a good therapeutic effect and it is worth popularizing.

Ceramide (Cer),as the center molecular of sphingolipids metabolism,functions as a bioactive mediator of different cellular processes,such as cell growth,differentiation,senescence and apoptosis,besides being structural component of cellular membrane.It can inhibit cell proliferation and promote cell apoptosis via multiple signal pathawys.Chemicals anti-cancer properties based on Cer metabolism that upregulate its level are confirmed in plenty of experimental researches,and parts of research productions are applied in anticancer clinic experiments.

At present, selective 5-hydroxytryptamine (5-HT) reuptake inhibitors (SSRIs) and 5-HT and noradrenaline (NA) re-uptake inhibitors ( SNaRI) are the first choice for the clinical treatment of depression, however, the clinical adverse effects of the drugs have become a prominent problem affecting the clinical efficacy. Chinese medicines have some experience in the treatment of depres-sion, and the combined use with the above antidepressants can enhance the efficacy and / or reduce the adverse effects. The article re-viewed the status of the combination of SSRIs and Chinese medicines in order to provide reference for the clinical treatment of depres-sion.

Intraperitoneal. injection of the essential oils of Asarum himalaicum and A. heterotropides var. mand shuricum can inhibit the proliferation of granuloma induced by implantation of cotton pellets in rats, and change the distribution of Copper and Zinc,the two necessary trace elements for rats,in rat serum and liver.They reduce concentration of Zinc but increase that of Copper in the serum,while elevate the concentration of Zinc but reduce that of Copper in liver. The oil of A. himalaicum collected from Ningxia Region showed even stronger effects.

A lot of progresses in understanding of the production and effects of NO have been made in recent years. Large number of studies showed that NO play an important role in the pathogenesis of septic shock. Some other investigators reported that NOS inhibitors could have the potential therapeutic value in septic shock treatment.

Objective To observe the effect of four-handed technique in light cured resin veneer in the treatment of dental fluorosis. Methods Eighty outpatients with dental fluorosis in our hospital from January 2011 to January 2014 were randomly divided into two groups: 40 in the study group were treated with four-handed technique and another 40 in the control group with one-doctor manual operation. The two groups were compared in terms of treatment duration and curative effect. Result The treatment duration in the study was significantly shorter than that of the control group and the curative effect was significantly better than that of the control group (all P<0.05). Conclusion As for the light cured resin veneer in the treatment of patients with dentalfluorosis in the process , the four-handed technique is better than one-doctor manual operation.

Objective To investigate the utilization of liver enhancement in hepatobiliary phase of gadolinium ethoxybenzyl diethylenetriamine pentaacetic acid (Gd-EOB-DTPA)-enhanced MRI for liver function evaluation.Methods Fifty-five patients who received enhanced MRI with Gd-EOB-DTPA were retrospectively analyzed.Images were obtained before injection and in hepatobiliary phase (5,10,and 20 minutes after Gd-EOB-DTPA injection).The patients were assigned into two subgroups according to individual liver function (n =35 in Group 1:normal liver and Child-Pugh class A; n =20 in Group 2:Child-Pugh class B and C).The relative liver enhancement (RE) was calculated at different time point.The general data (age,sex) and relevant laboratory results were recorded.Independent sample t-test was conducted to compare the RE between two groups at different time point.ROC curve was used to determine the best time point and RE threshold that can reflect the differences between two groups.Univariate analyses was performed to analyze the relationship between RE at the best time point and laboratory results.Multivariate analyses was performed to screen the independent influencing factor for RE at the best time point.Results The differences of RE between two groups were statistically significant at all time points (P ＜ 0.0001).10 minutes was the best time point for detecting the differences of liver function between two groups.When an RE cutoff value (＞ 1.52) was applied,normal or Child-Pugh class A could be predicted with sensitivity of 74.3％ and specificity of 90％.RE at the best time point was significantly related with total serum bilirubin level (TBil),serum albumin level (Alb) and prothrombin time (PT).And TBil was an independent influencing factor.Conclusion RE can be used to evaluate the liver function,and 10 minutes is the best time point that can be used to differentiate patients with normal or mild liver damage from those with moderate or severe liver damage.

Doxorubicin as a member of the anthracycline-based drugs, is widely used in cancer chemotherapy, while its significant cardiac toxicity and cytotoxicity limit its application to some extent.Therefore, it's a valuable exploring topic on how to improve the effect and reduce the adverse drug reactions of doxorubicin chemotherapy.This paper attempted to analyze the literatures about TCM combined with doxorubicin in nearly 15 years, including clinical use and experimental studies, to provide reference for the clinical application.