OObjective] Study on“Shanghuo”connotation,expression and its application of the Chinese medicine.[Method] Based on studying the ancient literature,we can define the“Shanghuo”disease concept according to the anatomical site at the head part,pathogenesis with“Shanghuo”pathological features;We can summarize the specific words of“Shanghuo”disease expression according to a variety of pathogenesis. [Results]The common terms of“Shanghuo”disease include“Shangchong”“Chongshang”“Shangyan”“Yanshang”“Shangfu”“Shanggong”“Shangyue”“Huoyan”“Huosheng”“Shengyan”“Shangzhuo”“Shangteng”“Shangxing”,they are thirteen kinds.The category of“Shanghuo”disease includes“epistaxis”“toothache”“sore throat”“aphtha”“ kakostomia”“tongue pain”“tinnitus”“headache”“facial acne”and more than 30 species.Clearing-heat and nourishing-yin is not the only treatment for“Shanghuo”being divided into Yinhuo and Yanghuo. [Conclusion]“Shanghuo”disease is generally expressed through the specific words,the latter is intrinsically linked with the fire of different organs,the different parts of disease and the nature of disease.Treatment of“Shanghuo”should be based on syndrome differentiation.

The concentrations of lactoferrin (LF) in colostrum during the first 8 days and mature milk during the first 4 months of postpartum were determined by electroimmunodiffusion. Meanwile a sensitive enzyme linked imm-unosorbent assay for measurements of LF in neonatal faeces were established. The results showed that human milk, especially colostrum, was rich in LF. The colostrum contained 6.88 ? 3.10mg/ml (M?SD) aad mature milk 3.18 ? 0.37mg/ml LF. LF in human milk was the highest during the first 3 days of lactation with a decline in concentration as lactation proceeds although a wide variation in concentration was noted among different individuals.LF in neonatal faeces showed significant difference in different feeding manners. LF level in the breast-fed infants was 19 times higher than that in the bottle-fed infants. The LF percentage detected in the former was 100% and the latter only 60%. The dynamic variations of LF in faeces of breast-fed infants during the first day of life were found to be relative to LF level in mother'milk.These data demonstrated the influence of high LF level in mother'milk on the concentrations of LF in breast-fed infants' intestine.Furthermore a few amounts of LF was found in meconium and its percentage detected was 35%. This suggested that the production of endogenous LF began from foetus period, but was limited during the first day of life. LF ingested by the breast-fed infants were not completely destroyed and thus may supplement the insufficiency of the endogenous LF in the newborn infants' intestinal tract.

Sodium orthovanadate is one of vanadate salts. When administered orally to STZ-induced diabetic rats, sodium orthovanadate may stimulate glucose uptake and. metabolism and made them normoglycemic. However, the blood glucose levels of normal rats treated with sodium orthovanadate did not change despite their plasma insulin levels decreased. The insulin-like effect of sodium orthovanadate on carbohydrate metabclism was not due to direct stimulation of insulin secretion, because the plasma insulin concentration of vanadate-treated STZ-rats remained lower and insulin secretion of isolated pancreas perfused with solution containing sodium orthovanadate was smilar to that of controls.

Objective] Study on the exact meaning of Quhan in traditional Chinese medicine processing. [Methods] Based on studying the ancient and modern Chinese literature, with Sichuan pepper as the breakthrough point, we can analyze Quhan method of processing and the role. [Results] The process of Quhan is a component of the water or oil that is contained in the internal drug, which is transferred to the surface of the drug by frying or heap stuffy, it can not simply be regarded as to remove the water or oil from the drug. A correct understanding of Quhan is practically significant for people to master some of the traditional Chinese medicine processing degree and improve the treatment result of the drug instead of removing the water or oil from the drug. [Conclusion] Quhan is the method that the liquid substance is free to the surface of traditional Chinese medicine processing, which can make the drug's effect better.

Axillary reverse mapping (ARM) is anatomic theoretical foundation based on breast and upper limb lymph axillary lymph injected into different lymphatic populations, ARM can display upper limb lymphatic channels through different tracers during the periods of axillary lymph node dissection and sentinel lymph node dissection, to reduce the rate of postoperative upper extremity edem.Although ARM has a positive effect, it exists a controversy in safety and the relationship between its node and sentinel lymph node.

BACKGROUND:Biological artificial skin made by fibroin, connective tissues, biopolymer materials, synthetic polymeric material, nano materials as wel as sensor and non-biological artificial skin both have achieved satisfactory effects in clinical trials, but there is stil a significant difference from the natural skin. OBJECTIVE:To investigate the treatment outcomes of epidermal stem cel s/porcine acel ular dermal tissue-engineered skin for ful-thickness skin defects in rats. METHODS:Twenty Sprague-Dawley rats were selected to make the skin defect model on the rat back, and then randomly divided into experimental and control groups, fol owed by subjected to the implantation of human epidermal stem cel s/porcine acel ular dermal tissue-engineered skin and porcine acel ular dermal matrix, respectively. Gross, histological and immunohistochemical observations were performed at 4 weeks after implantation.RESULTS AND CONCLUSION:Gross observation:the wound in the experimental group healed wel , and the skin had good elasticity;the control group showed scar formation in the implanted site and the texture was hard. Histological observation:there were good epidermal and dermal structures under microscope in both groups, and the basal layer, stratum corneum and stratum corneum of the epidermis could be distinguished clearly. Compared with the experimental group, more fibrous connective tissue could be found in the control group. Immunohistochemical observation:the wound surface in the experimental group was positive for the anti-HLA class I antigen, while the wound surface in the control group negative for the anti-HLA class I antigen. These findings suggest that the human epidermal stem cel s/porcine acel ular dermal tissue-engineered skin can effectively inhibit the scar formation and contracture in the repair of ful-thickness skin defects.

ObjectiveTo explore the effective method of intestinal irritable syndrome(IBS). Method Randomly divide 57 cases into 2 groups, Chinese drug group 28 cases were treated with Tongxie Yaofang; the combination group 29 with revised Tongxie Yaofang and chlorimipramine, for 4 weeks.Result The gastro-intestinal symptoms were relieved markedly for both groups; after treated for 14 and 28 days, there’s no statistical difference between the both groups on reduction rate of patients’ SDS; at the 28~ th day, there’s no obvious difference on the symptoms of abdomen pain and diarrhea in both groups. Conclusion Tongxie Yaofang can effectively treat IBS. The relieving of depression may not be related with chlorimipramine.

Objective:To study bacterial distribution and antimicrobial resistance of 489 strains of bacteria isolated from clinical urinary specimens of inpatients in department of kidney diseases of our hospital from 2001 to 2002,and propose rational use of antibiotics.Methods:Bacteria was isolated and identified by routine methods.Antimicrobial susceptibility was tested by K-B method.Results:in the 489 strains,there were307 strains of gram-regtive bacilli which account for 62.5%,mean while,there were 182 strains of gran-positive cocci which acconnt for 37.5%.The gram-negative bacilli mainly included K.pneumonice P.mirabilis and E.aerogenes.And the gram-positive cocci mainly included enterococcus and serum coagulase negative staphylococcus.The gram-negative bacilli were sensitive to imipenem and piperacillin/tazobactam,while the gram-positive cocci were sensitive to vancomycin and Teicoplamin.Conclusion:The urinary system infection is always induced by E.coli and Enterococcus.The periodical detection for bacterial resistance has an important significance for clinical treatment.

Objective To detect HBV outer membrane large protein(HBV-LP) and HBV-DNA, and to explore their correlation in judging the pathogenetic condition and HBV replication, as well as its clinical significance. Methods Serum level of HBV-DNA was quantitatively detected by u-sing real-time polymerase chain reaction, and while HBV-LP and HBV markers were measured with enzyme-linked immunosorbent assay(ELISA) in infected sera from 238 patients with hepatitis B. Re-suits There was significant difference of detection rate between HBV-DNA and HBV-LP(P<0. 05).The expression of HBV-DNA and HBV-LP had significantly difference among various patterns of HBV serological markers(P<0.05). HBV-LP expression was markedly correlated with the logarithm of HBV-DNA level(r=0. 677,P<0.01). Conclusion There is a prefect correlation between the copies of HBV-DNA and the level of HBV-LP, but HBV-LP expression can not still reflect the status ofHBV replication.

Objective To prepare Lingqiao Jiedu Pellets. Methods Lingqiao Jiedu Pellets was prepared by extrusion-spheronization method. Formulation composition and process factors influencing physical properties, such as spherical shape degree and productivity, were optimized. And it had a film coating by fluidized bed. Results Blend the extraction with MCC, and add adequate water. And squeeze with 30 r/min and roll 6 min with the rate of 48 Hz, drying and screening. Conclusion The pellets had a good appearance and stable quality.