OBJECTIVE:To prepare lansoprazole enteric-coated pellet capsules(LECPC).METHODS:The lansoprazole enteric-coated pellets were prepared using fluid-bed coating technology through blank pellets being coated with main component layer,insulating layer and enteric layer in order.Enteric-coated pellets were encapsulated into hard gelatin capsules to obtain LECPC.Drug-loading rate and drug release in artificial intestinal fluid and artificial gastric fluid of 3 batches were investigated.RESULTS:The prepared pellets assumed complete round shape,lustrous appearance and coating well with mean drug-loading rate above 96%.The release of 3 batches in artificial intestinal fluid within 45 minutes were greater than (94.3?0.76) % while were smaller than (6.2?1.6)% in artificial gastric fluid within 2 hours.CONCLUSION:The prepared capsule is feasible in technology,reproducible and reliable in quality.It represents satisfactory release in vitro and property of resisting acid.

OBJECTIVE:To prepare tacrolimus-hydroxypropyl-?-cyclodextrin inclusion compound and to investigate the solubility of inclusion compound.METHODS:The inclusion compound was prepared by agitation method and identified by differential scanning calorimetry and X-ray diffraction analysis respectively.The solubility of inclusion compound was evaluated by phase-solubility method.RESULTS:The solubility of tacrolimus in water could be improved remarkably by hydroxypropyl-?-cyclodextrin and increased along with the increase of hydroxypropyl-?-cyclodextrin concentration and testing temperature.The solubility of tacrolimus was increased from 2.07 ?g?mL-1 to 167.74 ?g?mL-1 when the concentration of hydroxypropyl-?-cyclodextrin was 0.50 g?mL-1 at 25 ℃.Results of differential scanning calorimetry and X-ray diffraction analysis indicated inclusion compound was prepared successfully.CONCLUSION:Hydroxypropyl-?-cyclodextrin could increase the solubility of tacrolimus.

Objective To study the relationship between plasma B-type natriuretic peptide(BNP) and the elderly patients with paroxysmal atrial fibrillation (AF). Methods The levels of plasma BNP in period of attack and cardioversion of the elderly patients with (AF)( n=45) were measured by immunofluorescence method ,and the tert results were compared with the healthy control group ( n = 30). Echocardiography was used to measure LAD and LVEF. Results The levels of plasma BNP in period of attack and cardioversion of AF group were significant higher than that in control group (P < 0. 05 ), and the period of attack was significant higher than that in the period of cardioversion(P < 0. 05); LVEF, LAD in period of attack and cardioversion of AF group were significant higher than that in control group(P < 0. 05). Conclusion High concentrations of plasma BNP level may promote the attack of AF,and they are associated closely with atrial remodeling and cardiac function.

Objective:To study the composition and distribution of two muscle fiber types in SD rat gastrocnemius muscle in order to learn its function.Methods:Gastrocnemius muscles of both sides of hind limbs were collected from 10 adult SD rats.8-?m-thick cryostat sections were transected and then stained with the method of myosin-ATPase following a procesure modified partially from one that Guth & Samaha reported before.Results:Gastrocnemius muscle from SD rats can be clearly identified with two different fiber types which are typeⅠ(slow twitch fiber)and typeⅡ(fast twitch fiber) by the myosin-ATPase staining method.Fibers of type I are stained white while fibers of type II are stained brown.The distribution pattern of two types of muscle fibers in gastrocnemius muscles is uniform and chessboard-like.The proportion of typeⅡmuscle fibers contained in the muscle is(72.2?6.8)%,much higher than that of typeⅠfibers which is(27.8?2.4)%(P

Antibodies, Monoclonal ; therapeutic use ; Drug Resistance ; Glucocorticoids ; therapeutic use ; Humans ; Immunoglobulins, Intravenous ; therapeutic use ; Infliximab ; Mucocutaneous Lymph Node Syndrome ; diagnosis ; drug therapy ; Plasma Exchange ; Tumor Necrosis Factor-alpha ; genetics

ObjectiveTo observe the effect of manipulation treatment in fricative training on Uygur dysarthria patients. Methods60 Uygur dysarthria patients were divided into phonetic training group and phonetic training adding manipulation treatment group. ResultsThe fricative of manipulation treatment group is better than phonetic training group in pronunciation. ConclusionThe manipulation treatment is needed in fricative training of Uygur dysarthria patients.

Objective To explore the effect of the Stroke Unit (SU) care on Uygur stroke patients and their prognosis.Methods The therapeutic effect of 153 Uygur stroke patients in general ward and 160 Uygur stroke patients in SU were compared.Results The patients in SU got a lower death rate and superior therapeutic effect compare with those in the general ward ( P<0.05～0.01).Conclusion The SU management model can produce a good effect on Uygur stroke patients.

Objective To evaluate the role of procalcitonin (PCT) in the diagnosis and treatment of infective endocarditis (IE).Methods It was a retrospective study of 20 cases diagnosed as IE with positive culture between June 2010 and June 2012 of First Affiliated Hospital of China Medical University,Department of Infectious Diseases.Results The concentration of C-Reactive Protein (CRP) and PCT in the dead group recorded on the first day and the fifth day in the treatment was significant higher than the survival group.The decreased degree of CRP and PCT concentration after treatment in the dead group was much deeper than the survival group.Moreover,the concentration of CRP and PCT in Staphylococcus aureus IE and negative bacilli IE were significant higher than Streptococcus viridans IE or Enterococcus faecalis IE,which was commonly above 2 μg/ml level.Conclusions Serum procalcitonin,lined with CRP,has the similar significance in the assessment of infective endocarditis severity,however,PCT is slightly better than CRP in assessing prognosis.The concentration of procalcitonin has implications to the strains of IE pathogen,in the mean time,it' s beneficial to guide the early use of antibiotics.

Objective:To compare the marginal microleakage of class V cavity restored with composite resin by 3 different methods. Methods:Class Ⅴ cavity was prepared in 36 human third molars,the teeth were randomly divided into 3 groups(n =1 2)and were repaired with common Z350 resin,pre-heated Z350 resin and Z350 resin inlay,respectively.After thermal cycling for 500 times the teeth were immersed in fuchsin solution for 24 h.Then the samples were sectioned longitudinally and examined for leakage under stereomicroscope.Results:The microleakage of cavity restoration by pre-heated Z350 resin and Z350 resin inlay was significantly low-er than that of the direct restoration with Z350 resin(P≤0.05).No significant difference was found between the groups of pre-heated Z350 resin and Z350 resin inlay.Conclusion:Marginal microleakage of Z350 resin restoration can be decreased by preheating of the resin.

Objective:To investigate the protective effects of quercetin ( QU) on myocardial ischemia/reperfusion ( MI/R) injury in rats and the underlying mechanisms. Methods:MI/R injury model in rats was prepared by coronary artery ligation for 30 min fol-lowed by 120 min reperfusion. And then the rats were randomly divided into 5 groups, QU (25, 50 and 100 mg·kg-1 ) groups, the model group and the sham group (n=10). QU was orally administered once a day for 7 days before the operation. The CK and LDH activities and TNF-α and IL-1β levels in serum were examined respectively by colorimetric and ELISA method after the reperfusion. Besides, the area of myocardial infarction was calculated and MPO activity in heart homogenate was detected. Results:QU (100 and 50 mg·kg-1 ) could significantly reduced the area of myocardial infarction to 25. 00% and 25. 31%, respectively, and compared with that of 32. 55% in the model group, the difference was significant (P<0. 05). The CK activity in the three QU groups was 779. 82, 793.90 and 870.86 U·L-1, respectively, which was significantly lower than that (1 123.78 U·L-1) in the model group (P<0. 01). The LDH activity in the three QU groups was 2 138. 21, 2 277. 40 and 2 416. 53 U·L-1, respectively, which was significant-ly lower than that (3 105.32 U·L-1) in the model group (P<0.05 or P<0.01). Compared with that (311.72 U·g-1) in the model group, the MPO activity in heart homogenate in the three QU groups was reduced to 185. 70, 190. 66 and 210. 03 U·g-1 , re-spectively (P<0. 05 or P<0. 01). Compared with that (119. 55 pg·ml-1) in the model group, QU (100 and 50 mg·kg-1) signif-icantly reduced the level of TNF-αto 97. 48 and 97. 54 pg·ml-1, respectively (P<0. 05). Compared with that (673. 34 pg·ml-1) in the model group, IL-1βlevel in serum in the three QU groups was evidently reduced to 419. 26, 438. 72 and 492. 53 pg·ml-1 , re-spectively (P<0. 01). Conclusion:The effectiveness of QU pretreatment in MI/R injury appears to be associated with the inhibition of neutrophil infiltration and expression of inflammatory cytokines.