The readers were divided into non-medical staff, scientific research medical staff, professional medical staff, practicing medical staff, patients and their family members using the user subdivision theory according to the subjects of service, characteristics of service, subject characteristics and information inquiry methods of readers. The service methods were innovated according to the characteristics of different readers and different measures were taken for meeting the demands of different readers,improving the satisfactory degree of readers and the service of library.

Acute lung injury is the result of organism autodestruction due to the mediators of inflammation overexpress. Recent studies suggest that GM-CSF correlate with alveolar macrophage function, lung host defense reaction and pulmonary surfactant homeostasis. GM-CSF influences regulation and signal transduction pathway of inflammation and apoptosis. These research progresses was been reviewed.

Objective To establish the determination of Dehydroandrographolide in Ganmaoqing Capsule. MethodsThe determination was carried out by HPLC with a KromasilC-18 column(250 mm ×4.6 mm,5μm),methanol-water(65:35)severed as the mobile phase,the speed was 1mL/min and the detection wavelength was at 254 nm. ResultsDehydroandrographolide showed a good linear relationship at the range of 0.04872 μg ～0.38976 μg(r2 =0.9995,n= 8);The average recovery of Dehydroandrographolide was 100.1%(RSD= 2.92%). ConclusionHPLC method was sensitive,accurate,reproducible,specific and could be used for quality control of Ganmaoqing Capsule efficiently.

With the rapidly development of the biotechnology industry,large quantities of recombinant proteins are needed for specific therapeutic and diagnostic applications.Bacterial cells are most often used for the production of recombinant proteins.However,recombinant proteins expressed in the cytoplasm of bacteria are often misfolded as insoluble inclusion bodies and therefore inactive.To circumvent this problem,several eukaryotic expression systems have also been developed over the years,ranging from yeast to mammalian cell-based technologies.For many mammalian proteins,especially those secreted and modified posttranslationally,a more compatible expression system is highly desirable because proper folding or modification can only be provided with closely related cells,i.e.,mammalian cells.Large scale transient transfection of mammalian cells is a recent and powerful technology for the fast production of milligram amounts of recombinant proteins.Transient expression by means of extrachromosomal replication in COS cells is frequently used to check the functional integrity of genes/plasmids and to produce small quantities of cell supernatants containing the protein of interest.As it is allowed for easy and efficient purification,many recombinant proteins used for therapeutic and structural studies are naturally secreted or engineered to be secreted.The use of a proper signal peptide is one of the major determinants for the efficient secretion of heterologous proteins from mammalian cells.The noncatalytic C-terminal hemopexin-like domain of MMP-2,PEX,can block angiogenesis and tumor growth in vivo.Large quantities of biochemically active recombinant PEX are required for the study of their functions and biochemical properties,as well as for their industrial applications.For this purpose,the rat growth hormone,mouse IgG? chain and MMP-9 signal peptides were used for expression of PEX in COS7 cells,and their secretion efficiencies were compared by Western blotting and ELISA.Western-blotting of PEX protein from culture media,resulted in detection of proteins with the predicted molecular mass,which indicate that all of the signal sequences could direct PEX secretion successfully.The MMP-9 signal peptide seems to be superior to the signal peptides from IgG and rGH both in terms of extracellular yield and in terms of secretion efficiency.Thus,expression of pM9PEX construct resulted in higher yields of extracellular PEX and the majority of the produced PEX was secreted and not trapped intracellularly.To examine whether the observed difference in secretion yields is promoted at the transcriptional level,a RT-PCR analysis was performed at 6 h after transfection.The presence of mRNA transcripts of PEX was observed in all the DNA constructs.Moreover,semiquantitative reverse transcription(RT-PCR)results show that there were no significant differences in the expression levels of PEX among the constructs at 6 h after transfection.Though there was no difference in the expression levels of PEX at an early time point after transfection,the presence of an ER-targeting signal peptide sequence in the expression vector affected the trafficking of expressed proteins in the cells.Hence,the described difference in exported yields is probably promoted at the secretion level,rather than at the transcriptional level.Chick chorioallantoic membrane(CAM)bioassay show that the PEX protein purified from cell culture had biological activity to inhibit the angiogenesis.The MMP-9's signal peptide is used for the first time as leader sequence for secretion of foreign proteins.The results revealed that higher amounts of secreted PEX were obtained when vectors containing MMP-9 signal peptide were used and it is also indicated that MMP-9 signal sequence could be effective on promoting the secretion of other heterologous proteins in eukaryotic cells.

Objective: To establish a method for the simultaneous determination of gallic acid(GA),methyl gal- late(MG)and ellagic acid(EA)in the residue of a gingival consolidation liquid(a mouth rinse preparation),and inves- tigate the effect of different residue-drying methods on the GA,MG and EA content in the residues. Methods: High per- formance liquid chromatography(HPLC)switching walvelength method was used to determine the GA,MG and EA con- tents. The column was Agilent Zorbax SB-C18(250 mm×4.6 mm,5 μm). The mobile phase was methanol(A)-0.05% phosphoric acid aqueous solution(B)in a gradient elution. The detection wavelength was 272 nm for GA/MG and 255 nm for EA. The flow rate was 1 ml/min,and the injection volume was 3 μl. Meanwhile,the residues were dried with the methods of sun drying,blast drying,vacuum drying and microwave vacuum drying,respectively and the GA,MG and EA contents in the residues were determined by the established HPLC method. Results: The linear ranges for GA,MG and EA were 1.280-4.608(r=0.9998),0.560-2.016(r=0.9998),0.1145-0.4122 μg(r=0.9997),respectively. The aver- age recoveries for GA,MG and EA were 99.97%,99.93% and 100.20%,with the RSD of 0.34%,2.30% and 0.93%,re- spectively. The contents of GA,MG and EA varied in quite a large range in the residues dried by different methods. Con- clusion: The established method is fast,simple and practicable,which could be used for the determination of GA,MG and EA in the residue of a gingival consolidation liquid. The drying methods could significantly affect the contents of GA, MG and EA in the residues,and the related results provide a reference for future studies.

Objective To explore the cardiac function of right ventricles in children with various course of bronchial asthma. Methods The cardiac function was evaluated by using the Doppler echocardiography in 36 chil-dren with course > 1 year bronchial asthma in remission stage and 38 children with course ≤ 1 year bronchial asthma in remission stage. 40 healthy children were used as normal controls. After treatment, 10 children with course > 1 year were evaluated again. Results The injury of right ventricle systolic function was predominant in children with > 1 year bronchial asthma, The systolic function of right ventricle remained normal in children with ≤ 1 year bronchi-al asthma. Conclusion The cardiac function damage occurs in children with > 1 year bronchial asthma and will be recoveied by cure the astham. The Doppler echocardiography is noninvasive method for determination of cardiac func-tion.

It is a brief introduction of necessity of uniform guidelines for pediatric emergency and critical care.Uniform guidelines are very important for development of discipline,safety of patients,protection of medical staff and administration.Uniform guidelines should be enacted according to our national condition with a scientific attitude of rigorous and gravity.

Objective: To study the plasma concentrations and pharmacokinetic parameters of 6,7 dimethoxycoumarin after oral administration of Herba Artemisiae Scopariae decoction and Yinchenhao decoction. Methods: A HPLC UV method was developed to determine 6,7 dimethoxycoumarin in rat plasma. The drug was extracted with acetonitrile from plasma and separated on a Kromasil ODS (250mm?4.6mm,5?m)column with methanol 1% solution of acetic acid THF (30∶63∶7) as mobile phase, UV detecter was set at 340nm. The data obtained were analyzed with Topfit program. Results: The liner calibration curves were obtained in the concentration range of 0.026～2.5 ?g?mL -1 . With non compartmental analysis, the main pharmacokinetic parameters after oral administration of Artemisiae Scopariae decoction and Yinchenhao decoction were as follows: T 1/2 was 1.30 and 1.75h, AUC 0→t was 1215.00 and 2527.85ng?h?mL -1 , AUC 0→∝ was 1325.90 and 2612.58ng?h?mL -1 respectively. Conclusion: This method can be used to study the pharmacokinetics of 6,7 dimethoxycoumarin derived from different traditional Chinese medicines containing this component.

Objective Hypothyroidism during pregnancy has been associated with impaired cognitive development and increased fetal mortality.In this retrospective study we attempt to identify the timing and adjustment of levothyroxine during pregnancy.Methods 20 women planning pregnancy after thyroidectomy because of thyroid cancer were observed before and throughout their pregnancies.Their thyroid function was measured before conception,and approximately every 4 weeks during pregnancy.The dose of levothyroxine was increased to maintain the thyrotropin concentration at preconception values throughout pregnancy.Results 21 pregnancies occurred in the 20 women and resulted in 20 full-term births.One woman had abortion.Their basal dose was all 100 μg.The dose increased by 12.5 μg a time to target the thyrotropin concentrations (0.1-0.25 mU/ml).The mean levothyroxine requirement increased 50 percent during the first half of pregnancy and plateaued by the 16th week.This increased dose was required until delivery.All the newborns and their mothers were healthy according to the follow-up.Conclusions We propose that women after thyroid cancer surgery should increase levothyroxine dose as soon as pregnancy is confirmed.Thereafter,serum thyrotropin level should be monitored and the levothyroxine dose adjusted accordingly.The adjustment dose is set at 12.5 μg and the maternal thyrotropin concentration is between 0.10 and 0.25 mU/ml,which guarantees healthy growth of fetus while suppresses thyroid tumor growth simultaneously.

On the basis of summarizing association rules algorithm and algorithm optimization,the paper explores the rules of prescription compatibility in the scheme for apoplexy diagnosis and treatment through Traditional Chinese Medicine (TCM),establishes the TCM Electronic Medical Records (EMR) database,processes and analyzes the data based on the classical association rule-Apriori algorithm,introduces the parameter optimization mining results of correlated measurement parameters Kulc and IR,eliminates pseudo strong association principle,gets the drug compatibility with strong correlation and theoretical significance,and provides theoretical guidance and reference for apoplexy medication through TCM.