Objective To explore the relationship of test anxiety status between the senior high school students and their parents before college examination entrance.And to study the method and effect of psychological intervention on test anxiety.Methods 315 grade 1 and 295 grade 3 of senior high school students were investigated by using Test Anxiety Scale(TAS),State Anxiety Inventory(S-AI) and Self-Rating Anxiety Scale(SAS);and their Darents were investigated by SAS,S-AI.57 grade 3 of senior high school students and their 102 parents were tested before and after psychological intervention.Results Grade 3 of senior high school students and their parents acquired higher SAS.S-AI scores than grade 1 students and their parents(t=2.422～3.975,P<0.05,P＜O.01).The TAS scores of grade 3 students were higher than grade 1(P＜O.01).The correlative coefficient between students and parents were 0.641,0.759,0.733,0.824 respectively(P<0.01).The TAS,SAS,S-AI scores of grade 3 of senior high school students and the SAS,S-AI scores of their parents were descended significantly [(41.12±10.13)vs(49.24±11.80),(43.05±9.97)vs(49.01±11.29),(53.28±9.87)vs(58.55±10.20),(52.47±9.91)vs(59.18±11.75),t=2.861～4.456,P＜O.01].Conclusion There was higher anxiety level in grade 3 of senior high school students and their parents.The correlations was strong between themPsychological intervention on students together with their parents are effective to relax test anxiety level of senior high school students before college examination entrance.

Objective:To evaluate the bio-mechanics of the cracked molars. Methods: A three dimensional finite element(3D-FEM) model of an upper first molar was constructed by Ansys 8.0 software. The model's cuspal inclination was shaped according to the mean value of the cuspal inclination in the cracked maxillary first molars and the intact ones respectively. The influence of cuspal inclination on the stress distribution was evaluated under different loads:Load of 600 N vertically applied to the occlusal surface of the model, or load of 200 N applied from three directions (vertically, at a 45?angle, horizontally). Results:For the model with the cuspal inclination of the cracked maxillary first molars, its maximum tensile stress, compressive stress and Von Mises stress were higher than those of the control models. The tensile stress and Von Mises stress increased with the increase of force direction and became to the highest under horizontal loads. The tensile stress was mainly distributed at the mediolingual cusp, centre groove and the palatal side of the cervical region. Conclusions: Under equivalent loads, the molar with bigger cuspal inclination suffers more unfavorable stress, which will magnify the occurrence of cracked tooth syndrome (CTS), and horizontal bite force may be a critical factor.

laboratorian should understand and evaluate them correctly.

[Objective]To introduce the clinical experience of Pro.Huo Jiege treating lung cancer in the elderly. [Methods]Through copying prescription with the teacher and carding records, and studing on Chinese literature, sum up Pro.Huo Jiege ’s unique insights for the etiology, pathogenesis, principles and methods, and explains it by one case. [Results] Pro.Huo Jiege thinks that the treatment of elderly lung cancer should base on syndrome differentiation, regulate spleen and stomach and nutrition to other organs at the same time.[Conclusion]The treatment of lung cancer in the elderly patients by Traditional Chinese medicine has achieved some effect in clinical practice and provides guidance in the clinical practice.

The new,rapid,accurate and convenient tests for tuberculosis diagnosis and drug resistance are urgently needed for effective tuberculosis control.In this review,two novel,inexpensive diagnosis methods of active TB were introduced.They can detect tuberculosis disease and drug resistances directly form sputum specimens and might have greater applicability in developing countries.Several concerns about the research and development of innovative diagnosis technology in our country also are described.

Spesis/severe sepsis remains a serious problem in pediatric intensive care unit.The mortality of sepsis/severe sepsis is still very high.The waterfall cascade reaction of cell factors is the main reason leading to the continued deterioration of the condition.Continuous blood purification technology can not only remove inflammatory mediators by adsorption and filtration function,but also improve the immune function,even reduce the mortality rate.But there is a controversial topic on the selection of the appropriate dose.This review not only introduces the effects of high-volume hemofiltration and low-volume hemofiltration from different aspects,but also analyses the influencing factors of different effects.

Adult ; Carbodiimides ; poisoning ; Humans ; Male ; Methylamines ; poisoning ; Occupational Exposure ; Young Adult

Objective To investigate clinical significance of serum retinol -binding protein(RBP)in chron-ic hepatitis B(CHB)and liver cirrhosis after hepatitis B infection(LC).Methods 109 hospitalized patients were involved in this study.They were 51 patients with CHB,58 patients with LC.56 healthy individuals were selected as the the health controls(HC).RBP,ALT,AST,GGT,LDH,TBIL,PA and ALB were analyzed by automatic biochemical analyzer.Results Decreased serum RBP was observed in patients with CHB and LC(CHB vs.HC:t =8.06,P <0.01;LC vs.HC:t =10.26,P <0.01).In addition,the concentration of serum RBP in group of LC was lower than that in CHB(t =3.41,P <0.01).RBP was positively correlated with PA in patients with CHB and LC(CHB:r =0.856,P <0.01;LC:r =0.737,P <0.01),and RBP was positively correlated with ALB in patients with CHB and LC (CHB:r =0.571,P <0.01;LC:r =0.328,P <0.05).ROC analysis showed that area under the receiver operator characteristic curve(AUC)in CHB and LC were 0.874 and 0.942,respectively.Conclusion Serum RBP decreases significantly in patients with CHB and LC.Serum level of RBP is associated with severity of liver diseases and maybe a potential prognostic index for liver disease.

Objective:To produce a fusion protein(ICOS-Ig)between extracellular portion of human ICOS and Fc portion of mouse IgG 2a and study on its biological activity.Methods:cDNA encoding the extracellular domain of human ICOS was prepared by RT-PCR from the RNA of the stimulated human peripherial blood mononuclear cells. The Fc portion of mouse IgG2a was cloned by PCR from the vector that contains the sequence-encoding Fc portion of mouse IgG2a.Two resulting amplified PCR products were ligated into a secrection mammalian expression vector, pSecTag2/Hygro A. The recombined vector was transfected into CHO cells by lipofectamine2000.Results:RT-PCR demonstrated the integration and mRNA synthesis of fusion gene. ELISA and Western blot analysis showed the expression of ICOS-Ig and its molecular weight was between 43-66 kD, its concentration was 5-25 ?g/ml. FACS analysis assured that ICOS-Ig had ligand specific binding activity. Addition of ICOS-Ig to MLR resulted in inhibition of T-cell proliferation and IL-2,IFN-? secretion.Conclusion:The fused gene ICOS-Ig was constructed and expressed successfully. It had the bioactivity of inhibition of T cell proliferation and cytokine secretion.

OBJECTIVE: To establish a RP-HPLC method for the determination of the content of Emodin in Fugan oral liquid.METHODS: The sample was separated on Zorbax SB-C18 column(150 mm?4.6 mm,5 ?m) with methanol-0.1%phosphoric acid solution(85∶15) as the mobile phase at a flow rate of 1.0 mL?min-1.The detection wavelength was set at 430 nm and the column temperature was 30 ℃.RESULTS: The calibration curve was linear at a range of 0.075 2 mg～0.376 0 mg(r=0.999 96) for the Emodin.The average recovery was 99.77% and the related standard deviation(RSD) was 1.03%(n=6).CONCLUSION: This method was proved to be simple,quick and reproducible and can be used for the quality control of Fugan oral liquid.