Bacterial infection is likely to develop into sepsis, which is regarded as the main reason for high mortality rate. High-density lipoprotein cholesterol (HDL-C) and its associated apolipoprotein can combine with lipopolysaccharide, regulate the body's inflammatory response and reduce the mortality, which can provide a new method for treatment of bacterial infection.

A large quantity of mice McAb ascites with monoclonal antibody (McAb) hybridoma cells separated from mice ascites was prepared. Ascites of mice inoculated with McAb hybridoma cells were collected, and the McAb hybridoma cells were isolated by centrifugation with lymphocyte separating fluid. McAb hybridoma cells then were injected into other mice abdominal cavity to prepare ascites again. McAb hybridoma cells separated from a mouse could be injected into the abdominal cavity of 5 mice.　Each mouse could produce 3.97 ml ascites on average. This method could be used to prepare large quantity of high titer mice monoclonal anti-HBs/a ascites.

Objective: To establish a new hepatocellular carcinoma cell line EHBC-512 and to analyze its biological property, so as to lay a foundation for future study. Methods: The specimen of liver cancer was resected during operation and was primary cultured. The morphology of cells was observed under optical and electron microscope. The cell cycle was examined with flow cytometry; the chromosome karyotype was also analyzed. The expression of AFP in the culture supernatant was detected by immunofluorescence and chemoluminescence. The cultured cells were implanted into nude mice for observation of tumor formation. Results: The cultured cell line had the character of liver cancer under light microscope, and had rich mitochondria, with obvious nuclear heteromorphism and a chromosome number of 110-120. The doubling time of the cells was 48 h, and the adherence rate was 90%. Immunofluorescence showed that the cells were positive of AFP and CK18 and negative of CEA and CK19. The production of AFP was higher and the content of AFP was higher than 1210 μg/L. The histology of the exografts was similar to that of the primary tumor. Conclusion: We have successfully established a new hepatocellular cell line EHBC-512, which can stably secrete AFP in vitro, providing a novel tool for liver cancer research.

BACKGROUND:The human body pulse signal can be regarded as the convolution of the heart excitation resource signal and the pulse transfer system. The backward signal was studied more before, but the research to the forward signal is not enough.OBJECTIVE: To extract the heart excitation source signals in human pulse wave.DESIGN: A randomized controlled design.SETTING: Second Affiliated Hospital of China Medical University;Biomedical Engineering institute of Shandong University.PARTICIPANTS: Health physical-examined persons in the Second Affiliated Hospital of China Medical University on March 11,2004 were recurited. RM6240 physiological signals collection system was adopted.METHODS: The collection of pulse wave in healthy persons was input into system-analysis mode and based on the principle of blind deconvolution and feasible arithmetic, heart source signals were obtained.MAIN OUTCOME MEASURES: ①Normal human pulse wave ②The heart excitation source signals.RESULTS: In the back-half segment of cepstrum (n＞ n0), periodic impulse was in accordance with the basic human pulse frequency. There was an excitation source in the pulse wave. If high-pass filter was defined to the signal (when n0 equals to 30 in the experimentation the result will be best.), we could get the heart excitation source signal after it was filtered, F transformation, index and inverse transformation.CONCLUSION: This method is practical and feasible and will provide a basis to further analyze pulse wave.

Objective To clarify the clinical distribution and antimicrobial resistance characteris-tic of bacterial biofilm during catheter-associated urinary tract infection,and to simulate biofilm "real state" in vivo. Methods Totally 120 patients with catheter-associated urinary tract infections (UTIs) were enrolled in the study. The urine specimens were collected for screening biofilm strains and the corresponding planktonic strains. The biofilm was detected with semi-quantitative detecting method. Antibiotics susceptibility test were performed on the biofilm bacteria to clarify the difference of drug resistance in common MH medium the between biofilm strains and the corresponding planktonic strains,as well as the difference of drug resistance of positive strains of the biofilm between Poloxamer medium and common MH medium. Results Totally 48 strains (48/120,40%) of biofilm bacteria were detected. The antibiotic susceptibility test of planktonic and biofilm bacteria in Mueller-Hinton agar showed no significant difference (P>0.05), while the antibiotic resistance of biofilm bacteria in Muel-ler-Hinton agar and Poloxamer hydrogel was statistically different (P<0.05),and the former was stronger. Conclusion The biofilm bacteria during the urinary tract infection were mainly Staphylococci and Enterococci. The antimicrobial resistance of planktonic and biofilm bacteria have no significant difference in vitro, h is speculated that Poloxamer media may simulate the real living environment of biofilm bacteria and display their "true" drug resistance.

Objective To investigate the association of esp, gelE, ebpA and QS-fsr system and biofilm formation in Enterococcus faecalis. Methods Totally 24 isolates of Enterococcus faecalis were collected from urine and catheter of clinical urine tract infection patients in Third Xiangya Hospital from Oct. 2007 to Jun. 2008, and were divided into biofilm group and non-biofilm group. The luminance ratios of esp, gelE, ebpA and fsrrB of Enterococcus faecalis in biofilm group and non-biofilm group were detected by RT-PCR. And the expression of esp, gelE, ebpA, fsrrB genes in different groups were detected by real-time PCR and were relatively quantitated through 2-△△Ct method. Moreover, the relevancies between that fourgenes and biofilm formation in Enterococcus faecalis were analyzed respectively. Results The expression of esp and ebpA in biofilm group were 298 times and 59 times more than the non-biofilm group. The expression level ofgelE and fsrB in biofilm group were 1/244 and 1/249 times less than the non-biofilm group, and the luminance ratios of esp, gelE, ebpA and fsrB were not significant between the two groups (rank sum was 92,79, 42 and 34 respectively,all P ＞ 0. 05 ). Conclusions The results showed that the biofilm formation in Enterococcus faecalis was promoted by esp and ebpA, and was inhabited by gelE and fsrB, which suggested that the expression of esp, ebpA and gelE genes was regulated by fsr system.

Informed consent is the important thesis in life ethics and life law,which gives the patients and experimenters the right of self-decision making on medical procedures and experiments.As the development of public health and public health ethics,informed consent has been paid more attention,especially on informed consent problems in epidemiology and public health practice.The requirement of informed consent varies in different research fields.According to different practice purposes,the researchers choose relevant applicable methods.

OBJECTIVE:To observe clinical efficacy and safety of triptorelin acetate in the treatment of endometriosis after lap-aroscopy. METHODS:196 cases of endometriosis were randomly divided into experimental group and control group with 98 cases in each group according to admission sequence. Both groups received laparoscopic surgery. After surgery,experimental group was given triptorelin acetate by intramuscular injection,3.75 mg/time,every 4 weeks;control group was given gestrinone orally,2.5 mg/time,twice a week,for 5 months. The ovulation,menstrual recovery and the occurrence of ADR were observed in 2 groups. The endometriosis relapse of 2 groups were followed up for 1 year and observed. RESULTS:After treatment,the ovulation recov-ery time and menstrual recovery time of experimental group were all shorter than those of control group,with statistical signifi-cance(P<0.05). The incidence of ADR in experimental group(17.34%)was significantly lower than in control group(35.71%), with statistical significance(P<0.05). During follow-up period,the relapse rate of experimental group(7.14%)was significantly lower than that of control group(18.37%),with statistical significance(P<0.05). CONCLUSIONS:After laparoscopic surgery, triptorelin acetate can significantly improve therapeutic efficacy of endometriosis,reduce the occurrence of ADR.

Objective To explore the value of angiographic diagnosis and interventional therapy for Takayasu arteritis. Methods Thirty-eight patients with Takayasu Arteritis (TA)(11 men, 27 woman; aged 8-54; average age, 30.5?18) undergone angiography were analyzed retrospectively. The angiographic appearances in all of the patients were observed dynamically in a double blind method by two experienced radiologists together and formed a consensus interpretation. Results According to the classification of Lupi-Herrea, typeⅠwas found in 11 cases, typeⅡin 15, typeⅢin 7 and typeⅣin 5 by angiography. Among 38 patients the arterial involvement ineluded subclavian artery in 20 cases (52.6%), the abdominal aorta in 15 cases (39.5%), the renal artery in 14 cases (36.8%), thoracic aorta in 10 cases (26.3%), carotid artery in 9 cases (23.7%), iliac artery in 9 cases (23.7%), axillary artery in 7 cases (18.4%), superior mesenteric artery in 4 cases (10.5%), coronary artery in 3 cases (7.9%), pulmonary artery in 3 cases (7.9%), and so on. Percutaneous transluminal angioplasty was performed in 22 cases and the stent placement was managed in 12 cases successfully. Conclusions Angiography is one of the ideal and reliable methods for the diagnosis and guidance of the interventional treatment for TA, and the latter is a mini-invasive and effective way for TA.

Objective To evaluate the killing effects of oxymatrine(OM) on human colon cancer cell line SW1116 and the mechanism of its anti-neoplastic effect. Methods Methyl thiazolyl tetrazolium analysis, flow cytometry , polymerase chain reaction(PCR)-enzyme linked immunosorbent assay (ELISA) and RT-PCR were used to detect the killing effects of OM and its influence on cell cycle dis- tribution , telomerase activity, expressions of human telomerase reverse transcriptase (hTERT), c-myc ,p53 and mad1 genes in SW1116 cells. Results OM exhibited dose-dependent killing effects on SW1116 cells and induced the increase of G1/G0-phase cells and decrease of S-phase cells. It was found that OM could supress the telomerase activity of SW1116 cells, and the effects were dose- and time-dependent. After OM administration, the expression of hTERT gene in SW1116 cells was decreased, those of p53 and mad1 genes were increased, and the expression of c-myc gene had no marked changes. Conclusions OM has dose-dependent killing effects on SW1116 cells.The anti-neoplastic activity of OM might be due to the inhibition of telomerase activity by means of its influence on hTERT and the up-stream regulation genes.