A large quantity of mice McAb ascites with monoclonal antibody (McAb) hybridoma cells separated from mice ascites was prepared. Ascites of mice inoculated with McAb hybridoma cells were collected, and the McAb hybridoma cells were isolated by centrifugation with lymphocyte separating fluid. McAb hybridoma cells then were injected into other mice abdominal cavity to prepare ascites again. McAb hybridoma cells separated from a mouse could be injected into the abdominal cavity of 5 mice.　Each mouse could produce 3.97 ml ascites on average. This method could be used to prepare large quantity of high titer mice monoclonal anti-HBs/a ascites.

Bacterial infection is likely to develop into sepsis, which is regarded as the main reason for high mortality rate. High-density lipoprotein cholesterol (HDL-C) and its associated apolipoprotein can combine with lipopolysaccharide, regulate the body's inflammatory response and reduce the mortality, which can provide a new method for treatment of bacterial infection.

Objective: To establish a new hepatocellular carcinoma cell line EHBC-512 and to analyze its biological property, so as to lay a foundation for future study. Methods: The specimen of liver cancer was resected during operation and was primary cultured. The morphology of cells was observed under optical and electron microscope. The cell cycle was examined with flow cytometry; the chromosome karyotype was also analyzed. The expression of AFP in the culture supernatant was detected by immunofluorescence and chemoluminescence. The cultured cells were implanted into nude mice for observation of tumor formation. Results: The cultured cell line had the character of liver cancer under light microscope, and had rich mitochondria, with obvious nuclear heteromorphism and a chromosome number of 110-120. The doubling time of the cells was 48 h, and the adherence rate was 90%. Immunofluorescence showed that the cells were positive of AFP and CK18 and negative of CEA and CK19. The production of AFP was higher and the content of AFP was higher than 1210 μg/L. The histology of the exografts was similar to that of the primary tumor. Conclusion: We have successfully established a new hepatocellular cell line EHBC-512, which can stably secrete AFP in vitro, providing a novel tool for liver cancer research.

Objective:To investigate the relationship of etiology and complications of rhabdomyolysis with its prognosis in the elderly.Methods:Patients with rhabdomyolysis at the emergency department of our hospital from January 1, 2018 to December 31, 2019 were retrospectively analyzed.Based on age, patients were divided into the non-elderly group(<65 years old)and the elderly group(≥65 years old). The frequency distribution of etiological factors, concurrent acute kidney injury, and their association with prognosis were analyzed.Results:The number of patients with rhabdomyolysis caused by 2 or more etiologies was higher in the elderly group than in the non-elderly group(40.3% or 48/119 vs.17.0% or 16/94, χ2=13.582, P=0.000). The frequency distribution of etiological factors was different between the two groups.The top-five etiologies were infection, muscle ischemia/hypoxia, endocrine metabolic abnormalities, trauma and muscle fatigue in the elderly group and muscle fatigue, infection, endocrine metabolic abnormalities, drugs/toxicants and trauma in the non-elderly group.Compared with the non-elderly group, the elderly group had fewer patients with typical clinical manifestations(32.8% or 39/119 vs.48.9% or 46/94, χ2=5.067, P=0.024). In contrast, patients who newly presented with disturbance of consciousness were more likely to be found in the elderly group than in the non-elderly group(40.3% or 48/119 vs.21.3% or 20/94)( χ2=7.923, P=0.005). There were 37 patients with AKI(38.9% or 37/95)in the elderly group and 13 of them died(35.1%), and there were 17 patients with AKI in the non-elderly group(19.3% or 17/88)and 4 died(23.5%), indicating the elderly were prone to AKI( χ2=7.545, P=0.006). There was a significant correlation between AKI and prognosis in the non-elderly group( χ2=7.196, P=0.007). Conclusions:Rhabdomyolysis caused by multiple etiologies is more common in elderly patients than in non-elderly patients.The etiological classification of rhabdomyolysis in the elderly is different from that in the non-elderly.Elderly patients are less likely to have typical clinical manifestations and are more prone to AKI.Elderly patients with rhabdomyolysis combined with AKI have a poor prognosis.

Objective To investigate the association of esp, gelE, ebpA and QS-fsr system and biofilm formation in Enterococcus faecalis. Methods Totally 24 isolates of Enterococcus faecalis were collected from urine and catheter of clinical urine tract infection patients in Third Xiangya Hospital from Oct. 2007 to Jun. 2008, and were divided into biofilm group and non-biofilm group. The luminance ratios of esp, gelE, ebpA and fsrrB of Enterococcus faecalis in biofilm group and non-biofilm group were detected by RT-PCR. And the expression of esp, gelE, ebpA, fsrrB genes in different groups were detected by real-time PCR and were relatively quantitated through 2-△△Ct method. Moreover, the relevancies between that fourgenes and biofilm formation in Enterococcus faecalis were analyzed respectively. Results The expression of esp and ebpA in biofilm group were 298 times and 59 times more than the non-biofilm group. The expression level ofgelE and fsrB in biofilm group were 1/244 and 1/249 times less than the non-biofilm group, and the luminance ratios of esp, gelE, ebpA and fsrB were not significant between the two groups (rank sum was 92,79, 42 and 34 respectively,all P ＞ 0. 05 ). Conclusions The results showed that the biofilm formation in Enterococcus faecalis was promoted by esp and ebpA, and was inhabited by gelE and fsrB, which suggested that the expression of esp, ebpA and gelE genes was regulated by fsr system.

Objective To clarify the clinical distribution and antimicrobial resistance characteris-tic of bacterial biofilm during catheter-associated urinary tract infection,and to simulate biofilm "real state" in vivo. Methods Totally 120 patients with catheter-associated urinary tract infections (UTIs) were enrolled in the study. The urine specimens were collected for screening biofilm strains and the corresponding planktonic strains. The biofilm was detected with semi-quantitative detecting method. Antibiotics susceptibility test were performed on the biofilm bacteria to clarify the difference of drug resistance in common MH medium the between biofilm strains and the corresponding planktonic strains,as well as the difference of drug resistance of positive strains of the biofilm between Poloxamer medium and common MH medium. Results Totally 48 strains (48/120,40%) of biofilm bacteria were detected. The antibiotic susceptibility test of planktonic and biofilm bacteria in Mueller-Hinton agar showed no significant difference (P>0.05), while the antibiotic resistance of biofilm bacteria in Muel-ler-Hinton agar and Poloxamer hydrogel was statistically different (P<0.05),and the former was stronger. Conclusion The biofilm bacteria during the urinary tract infection were mainly Staphylococci and Enterococci. The antimicrobial resistance of planktonic and biofilm bacteria have no significant difference in vitro, h is speculated that Poloxamer media may simulate the real living environment of biofilm bacteria and display their "true" drug resistance.

BACKGROUND:The human body pulse signal can be regarded as the convolution of the heart excitation resource signal and the pulse transfer system. The backward signal was studied more before, but the research to the forward signal is not enough.OBJECTIVE: To extract the heart excitation source signals in human pulse wave.DESIGN: A randomized controlled design.SETTING: Second Affiliated Hospital of China Medical University;Biomedical Engineering institute of Shandong University.PARTICIPANTS: Health physical-examined persons in the Second Affiliated Hospital of China Medical University on March 11,2004 were recurited. RM6240 physiological signals collection system was adopted.METHODS: The collection of pulse wave in healthy persons was input into system-analysis mode and based on the principle of blind deconvolution and feasible arithmetic, heart source signals were obtained.MAIN OUTCOME MEASURES: ①Normal human pulse wave ②The heart excitation source signals.RESULTS: In the back-half segment of cepstrum (n＞ n0), periodic impulse was in accordance with the basic human pulse frequency. There was an excitation source in the pulse wave. If high-pass filter was defined to the signal (when n0 equals to 30 in the experimentation the result will be best.), we could get the heart excitation source signal after it was filtered, F transformation, index and inverse transformation.CONCLUSION: This method is practical and feasible and will provide a basis to further analyze pulse wave.

Because of the aggressive threaten of heat stroke and a lack of understanding of the mechanism of action, mammal animal models for experimental heat stroke were well developed. During the past 5 decades, anesthetized mouse, rat, rabbit, dog, baboon and monkey were used as animal model for experimental heat stroke. However, anesthetized mammals models have some limitations, such as neuroprotective effect of anesthetic agents, possible disturbance on injury and recovery of stroke animals by anesthetic agents, difficulty of discussing animal behavior before and after heat stroke, it was also difficult for the models to evaluate cognitive function of animal under hot environment. Considering humanitarian, only awaked and unrestrained mouse heat stroke model was accepted so far. Therefore, we also developed an awaked and unrestrained rat heat stroke model, and found it was helpful to evaluate drug effectiveness for animal behavior and cognitive function under hot environment.
Animals ; Cognition ; Disease Models, Animal ; Heat Stroke ; physiopathology ; Mice ; Rats

In this paper, we present a 3D virtual phantom design software, which was developed based on object-oriented programming methodology and dedicated to medical physics research. This software was named Magical Phan tom (MPhantom), which is composed of 3D visual builder module and virtual CT scanner. The users can conveniently construct any complex 3D phantom, and then export the phantom as DICOM 3.0 CT images. MPhantom is a user-friendly and powerful software for 3D phantom configuration, and has passed the real scene's application test. MPhantom will accelerate the Monte Carlo simulation for dose calculation in radiation therapy and X ray imaging reconstruction algorithm research.
Algorithms ; Humans ; Image Processing, Computer-Assisted ; Monte Carlo Method ; Phantoms, Imaging ; Software ; Software Design

Objective To explore the value of imaging diagnosis on complications after kidney transplantation. Methods The radiologic appearances of 165 patients with postoperative complications were reviewed in a double blind method by two experienced radiologists. Results In the 158 patients with postoperative pulmonary infections, the main etiological causes according to their frequency were bacteria,fungus,virus,and so on. The postoperative pulmonary infections of the patients with renal transplantation were diagnosed as normal(28,17 7%),increased lung markings(44,27 8%) and pulmonary infection(84,53 2%) by chest X-rays and CT. The renal artery stenosis after kidney transplantation was diagnosed in 5 cases, and thrombosis of pancreas blood vessel after operation of simultaneous pancreas-kidney transplantation was diagnosed in 1 case by DSA. Conclusions Radiography and CT scan are very useful in the clinical diagnosis of postoperative pulmonary infections in the patients with renal transplantation,and radiography should be the first choice for the imaging diagnosis. DSA is one of the ideal and reliable measns to diagnose the postoperative vascular complications in kidney transplant recipients.