In order to explore the cellular mechanism of antishock effect of anisodamine (654-2), the changes of intramitochondrial magnesium during late hemorrhagic shock were observed. Results showed that there exists a negative correlation between the blood plasma magnesium concentration and arterial blood pressure in shocked animals and at the same time the magneium content of liver mitochondria reduced significantly; 654-2 prevented the decrease of mitochondria magnesium and the increase of blood plasma magnesium, and improved the prognosis of the shocked animals. These results indicate that 654-2 may protect the liver mitochondria of shocked animals at a cellular and subcellular levels.

Hand, foot, and mouth disease (HFMD) is a viral infectious disease regarded to be a public-health problem worldwide. Since the 1990s, HFMD began to spread in the Asia-Pacific region (especially in South-East Asia). HFMD outbreaks have occurred in mainland China frequently since 2008, and the morbidity and mortality of HFMD has continued to increase in recent years. In mainland China, enterovirus A serotype enterovirus A71 (EV-A71) and coxsackievirus A16 (CVA16) have been the major pathogens of HFMD during these years. However, the etiological spectrum of HFMD changes with time. This review focuses mainly on the etiological spectrum of HFMD and changes in epidemic patterns in mainland China.
China ; epidemiology ; Disease Outbreaks ; Enterovirus ; classification ; genetics ; isolation & purification ; Hand, Foot and Mouth Disease ; epidemiology ; virology ; Humans ; Prevalence

Objective To investigate the characteristics and diagnostic value of fundus fluorescein angiography(FFA)for familial exudative vitreoretinopathy(FEVR).Methods 34 children(68 eyes)with FEVR and 64 parents(1 28 eyes)were included.All the clients were received examinations of slit-lamp biomicroscopy and indirect ophthalmoscopy.Meanwhile the children were examined by Retcam Ⅱ,the best corrected visual acuity of parents were recorded.The children and their parents were classified according to the ocular findings.Among 68 eyes of children,3 eyes(4.41% )were normal,4 eyes(5.88% )were in stage 1,7 eyes(10.29% )were in stage 2,2 eyes(2.94% )were in stage 3,8 eyes(11.76% )were in stage 4 and 44 eyes(64.71% )were in stage 5.Among 128 eyes of parents,74 eyes(57.81% )were normal,51eyes(39.84% )were in stage 1,1 eyes(O.78% )were in stage 2 and 2 eyes(1.56% )were in stage 5.FFA was performed on the children with RetcamⅡunder anesthesia and on the parents with HR2 in order to observe the FFA characteristics in different stage.Results FFA characte ristics in children included uncompleted vascularization of the periphery,peripheral avascular zone(stage 1);neovascularization and/or peripheral subretinal and intraretinal exudation(stage 2);subtotal retinal detachment with attached foyea (stage 3);subtotal retinal detachment with detached foyea(stage 4)and total retinal detachment(stage 5).FFA characteristics in parents included abrupt cessation of the peripheral retinal capillary network and a peripheral avascular zone(stage 1); abnormal peripheral arteriovenous shunts, neovascularization or exudation(stage 2)and atrophia bulbi(stage 5).Conclusions FEVR in different stage has different FFA characteristics.FFA plays an important role in early diagnosis of FEVR.

[Objective]To probe the research and treatment for Hashimoto thyroiditis in Th1/Th2 migration.[Methods]By referring to related literature from both home and abroad,the author tries to analyze and sort the literature and comes to a reasonable conclusion. [Result]There is a lot of experimental re-search on correction of Hashimoto thyroiditis Th1/Th2 migration at home and abroad,but less clinical treatment research.[Conclusion]Th1/Th2 cellmigra-tion is one of the reasons for Hashimoto thyroiditis.It can promote the development of the disease, have something to do with the rise of thyroid autoanti-bodies.

Objective To evaluate the clinical value and safety of radiotherapy combined with tyrosine kinase inhibitors (TKI) in non-small cell lung cancer (NSCLC) with brain metastasis driven by epidermal growth factor receptor (EGFR) mutation.Methods A retrospective cohort of 21 non-small cell lung cancer (NSCLC) patients with EGFR mutation-driven brain metastasis was recruited.10 patients (treatment group) were randomly selected for radiotherapy combined with TKI treatment,and 11 patients (control group) were treated with radiotherapy.Tumor size was measured and clinical efficacy was evaluated by MRI in the four weeks after radiotherapy.Since then,a clinical response was evaluated every three months until disease progression,and the median overall survival time was calculated.Results The objective remission was 8 cases in treatment group and 4 cases in control group.The disease control was 9 cases in treatment group and 8 cases in control group.The median overall survival time of treatment and control groups was 11.2 months and 6.4 months,respectively.Adverse reactions in treatment group mainly contained nausea,diarrhea and rash,which all were tolerated.Conclusion Radiotherapy combined with TKI is superior to radiotherapy alone in effect and safety for treatment of NSCLC with EGFR mutation-driven brain metastasis.

In this study, the influence of the duration of opening vestibular window and the application of suction in auditory bulla on cochlear function and ultrastructures were observed by ECochG and scanning and transmission election microscopy in 44 guinea pigs divided into six groups. The results were as follows: (1) The CAP thresholds progressively enhanced as the prolongation of duration in some of outer hair cells in the basal turn occured 180 minutes after total stapedectomy. (2)Significant CAP threshold shift and irreversible pathological changes in some outer hair cells in the basal turn of cochlea were found with continuous suction for 60 minutes in auditory bulla after total stapedectomy. (3)CAP threshold shift without damage of outer hair cells could be fourd with the application of suction for 60 mimutes in auditory bulla before stapedectomy. The results suggested that opening vestibular window for longer time with suction in auditory bulla might be one of the factors to cause postoperative sensorineural hearing loss in total stapedectomy. But the suction noise might not be enough to cause postoperative sensorineural hearing loss in tympanoplasty due to its relatively low intensity.

With the development of modem tumor diagnostic techniques and therapies, the survival rate of malignant tumor has been remarkably increased. With the increase in survival rate of cancer-beating patients, the chance of tumor metastasis is elevated. Minimally invasive treatment provides a new strategy to the therapy of spinal metastatic carcinoma. Its benefits include smaller surgical wound,, higher efficacy and fewer complications, and thus have a broad application prospects in the field of spinal metastatic tumors. In this article, a general re-view is given on minimally invasive treatments of spinal metastatic carcinoma, as well as the latest progress in the field. It was proposed that the united application of the treatment might be a promising hot spot of tumor therapy.

Tension-free hernioptasty is a major method for treating inguinal hernia, characterizing by less wound, tension-free incision, sample processing, less dissection and separation, mild pain, rapid recovery, and short length of stay. In particular, transabdominal preperitoneal inguinal hernioplasty is considered as the most ideal method at physiologicoanatomical level. However, the effect of patch materials on postoperative recovery is generally ignored for a long time. Recent studies demonstrate that patch weight and mesh diameter are important factors for abdominal wall repairing and reconstruction. Polypropylene patch, characterizing by strong stability, high intensity, great unreactiveness, and sample processing, has become a major material for hernioptasty. However, there are still some problems of clinical application, including that polypropylene patch may induce intensive foreign body reaction and chronic inflammation as well as compliance decreasing of abdominal wall. Therefore, polypropylene patch should be chosen according to patient requirement, i.e., light, soft, and wide-aperture patch, so as to reduce onset of complication. Nerve and ligament tissues could be clearly observed from light and soft patch, and this might and prevent hurt by mistake, improve repairing operability, enhance correct putting of patch, and decrease incidence of pain. Furthermore, the structure was beneficial for in-growth of fiber tissue, longitudinal and transversal movement induced by body position alternation and muscle contraction, and reduction of discomfortableness.

Poly lactic-co-glycolic acid (PLGA) has been attracting increasing attention in the field of bone tissue engineering because it is a biodegradable polymer with excellent biocompatibility, controllable aperture and porous rate, and a degradation rate which is suitable for bone regeneration, and moreover a suitable carrier for the sustained release of growth factors. This article reviewed the recent research progress in PLGA as a material for controlled drug delivery with emphasis on how to make the scaffold more suitable for the regeneration of the bone and which is the better way to apply sustained release of growth factors embedded in the material in bone tissue engineering. It pointed out that PLGA carrying different growth factors will become the focal point in the field of bone tissue-engineering.

Objective To analyze the constitutes of essential oil form Nervilia fordii. Methods GC-MS was used to analyze the essential oil. The relative content of each component was determined by area normalization. Results Fifty-five kinds of constitutes were identified,accounting 97.1 %of total contents. The content of 2-Pentadecanone,6,10,14-trimethyl is the highest,being 13.55 %,which had also been found in the same family. Conclusion 2-Pentadecanone,6,10,14-trimethyl can be used for the identification of the same family.