Cytochrome P450(CYP450)is the most important family of enzymes in microsomal mixedfunction oxidase,widely distributed in vivo.CYP450 is involved in the metabolism of many exogenous compounds,the generation of endogenous substances,in particularly,affecting the occurrence and development of the tumors and their drug treatment,in the fields of medical and pharmaceutical research,cytochrome P450 has been very noticeable.

Objective of electroacupuncture(EA) of Alzheimer′s disease(AD) rat learning and memory ability and plasma SOD , MDA content in brain P53 ,P21 expression .Methods The normal 36 SD rats ,were randomly divided into sham operation group , model group and EA group .EA group acupuncture “Baihui” ,“Taixi” ,“Zusanli” treatment ,through the Morris water maze test in rats behavioral changes ,spectrophotometric observation of SOD and MDA in rat plasma levels ;observation of rat brain P53 ,P21 ex-pression by immunofluorescence .Results Compared With the model group ,group EA escape latency ,longer in the third quadrant of the activities of the time ,the number of crossing platform position increased ,decreased the elevated plasma SOD level ,MDA lev-el ,reduce the expression of brain P53 ,P21 ,and the difference was statistically significant (P<0 .01) .Conclusion electro-acupunc-ture treatment can improve the Aβ1-42-induced AD rat spatial learning and memory ability ,may be lowered P53 ,P21 protein ex-pression increase SOD activity and MDA .

Objective To study the correlation between diabetes and the new discovered angiopietin-related growth factor (AGF)that participates in energy metabolism.MethodsA total of 24 male C.57BL/6 mice were divided into three groups:controlled group; fed with high-fat diet( HFD); fed with high-fat diet for 10 weeks and then treated with rosiglitazone ( HFD + RSG) ; The relative level of AGF mRNA expression in livers was measured by determining a ratio of PCR products of AGF to that of β-actin gene.Oral glucose tolerance test,and insulin resistance index (HOMA-IR) were used to measure blood glucose level and insulin sensitivity.Results Compared with the controls,the expression of AGF mRNA in liver decreased significantly in HFD group( P ＜0.05),and increased significantly in HFD + RSG group( P＜0.01 ).A negative correlation was found between AGF mRNA expression in mice livers and HOMA score ( r =-0.516,P ＜ 0.05 ).Conclusions AGF as a new hepatocyte derived circulating factor counteracts obesity and is related to insulin resistance.

The procedure of occlusal treatment involves improving the morphology and the stomatognathic function.Several practical methods and morphological endpoints have been described in occlusal rehabilitation.We made a selection of these (mandibular position,oc-clusal plane,occlusal guidance,occlusal contact,face-bow transfer,use of an adjustable articulator and occlusal support)and performed a literature review to verify the existence of compelling scientific evidence for each of these.A literature search was conducted using Medline /PubMed in March 2011.Over 400 abstracts were reviewed,and more than 50 manuscripts selected.An additional hand search was also con-ducted.Of the many studies investigating stomatognathic function in relation to specific occlusal schemes,most studies were poorly designed and of low quality,thus yielding ambiguous results.Overall,there is no scientific evidence that supports any specific occlusal scheme being superior to others in terms of improving stomatognathic function,nor that sophisticated methods are superior to simpler ones in terms of clini-cal outcomes.However,it is obvious that the art of occlusal rehabilitation requires accurate,reproducible,easy and quick procedures to re-duce unnecessary technical failures and /or the requirement for compensatory adjustments.Therefore,despite the lack of scientific evidence for specific treatments,the acquisition of these general skills by dentists and attaining profound knowledge and skills in postgraduate training will be necessary for specialists in charge of complicated cases.

Objective: To study the effect of volatile oils from Foeniculum Vulgare Mill, anisaldehyde, anethole and water extracts on the percutaneous penetration of 5 fluorouracil. Methods: With Valia Chien horizontal diffusion cell and HPLC method to observe the effect of volatile oils of Foeniculum Vulgare Mill, anisaldehyde, anethole on the percutaneous penetration of 5 fluorouracil. Results: The volatile oils of Foeniculum Vulgare Mill, anethole, anisaldehyde possessed the high promoting action on percutaneous absorption of 5 fluorouracil, and their enhancement ratio are 7.14, 4.17, 9.54, respectively. Conclusion: The volatile oils of Foeniculum Vulgare Mill, anethole, anisaldehyde enhance the skin permeation of 5 fluorouracil effectively.

Objective To explore the mechanism ofTiaozhong Huatan Anshen Mixture (THAM). Methods A total of 200 healthy Kunming mice were randomly and equally divided into first, second, third and fourth major groups. Each major group was further equally divided into blank group, control group and high, medium, low dose THAM groups. Each group was given an equal volume of NS, estazolam, or high, medium, or low dose THAM by gavage for 7 d. For the first major group, the changes in number of spontaneous activities after administration were evaluated. For the second major group, 60 min after administration, each group was given pentobarbital sodium (30 mg/kg body weight, minimum dose for all mice sleeping) by intraperitoneal injection, and the sleep time was recorded. For the third major group, 60 min after administration, each group was given pentobarbital sodium (15 mg/kg body weight, maximum dose for 90%-100% of all mice having righting reflex) by intraperitoneal injection. The number of mice falling asleep was recorded, and the sleep rate was calculated. For the fourth major group, mice were sacrificed after continuous administration 15 d. They were quickly removed brain tissue;homogenates were taken out in batches;5-HT and 5-HIAA levels in brain tissues were determined according to fluorescence spectrophotometric.Results Compared with the blank group, all treated groups had a reduced number of spontaneous activities, a shortened sleep latency, a prolonged sleep time, and an increased sleep rate after administration (P<0.01,P<0.05), with the most significant changes seen in the control group and high-dose THAM group (P<0.05), with out statistical significance between the two groups (P>0.05). Compared with the blank group, brain 5-HT and 5-HIAA levels in all treated groups increased in mice (P<0.01,P<0.05), with the most significant changes seen in the high-dose THAM group (P<0.05).Conclusion THAM has a significant sedative-hypnotic effect. The mechanism may be related to increasing brain 5-HT synthesis and metabolism.

Objective To study the effects of ceftriaxone sodium(Cef) on the seizures and the expression of glutamate transporter (GLT-1) in kainic acid (KA) epilepsy model.Methods Firstly, a chronic spontaneous seizure mouse model was established by unilateral hippocampal injection of KA and monitored by vEEG technique to record seizures.The experimental group received intraperitoneal injection of Cef 200 mg/(kg·d) and the control group received normal saline.Seizure frequency, interictal spike waves and histological phenotypes were recorded to evaluate the function of Cef.Then we use the Western blot to detect the effect of expression for GLT-1.Results Unilateral hippocampal injection of KA 200 ng successfully established the mesial temporal lobe epilepsy model.Cef can reduce the seizures from 2.145 times/day to 1.597 times/day, decreased by 31.2% with a statistical significance(P<0.05).Cef treatment did not significantly enhance the expression of GLT-1.Conclusions Intraperitoneal injection of Cef partially inhibites the seizures of KA model, but the expression of GLT-1 in hippocampus is not enhanced.It is suggested that ceftriaxone may inhibit seizures through other mechanisms.

Objective To explore the difference between the results of the cross-disciplinary joint teaching method and the traditional teaching method in medical humanity courses.Method 128 students in one military medical university were selected to be randomly assigned to the study group and the control group,using the cross-disciplinary joint teaching mode (study group,n=64) and traditional teaching mode (control group,n=64) in medical humanity courses.For the joint teaching mode,we compiled the teaching materials of medical humanities,formed the teaching team and organized the academic communication after class by social media.After all courses were finished,the subject cognition and approval of whole students were evaluated by the unified arranged examination and questionnaire.Then the data was processed by SPSS 18.0.Results On the aspect of subject cognition,the total scores of the study group were higher than control group in health law [(76.2 ± 3.34) vs.(72.5 ± 4.76);t=3.32,P=0.000] and medical ethics [(75.3 ±2.93) vs.(68.3 ± 3.10);t=4.01,P=0.000].On the aspect of subject approval,the recognition of the students of the study group in these three subjects were more than those of the control group except the selfevaluation of medical psychology (P=0.000).Conclusion The application of cross-disciplinary joint teaching method on medical humanity courses contributes evidently to the promotion of the medical humanity quality of clinical medical students.To implement this model,however,multi-sided resources are needed for colleges to coor-dinate,for perfect results require more practical exploration.

Objective To observe the influence of cervical traction and manipulation on hemorheology of cervical vertigo patients.Methods 59 patients in outpatients of our hospital from January 2012 to August 2013 were recruited and divided into a control group(29 patients)and a treatment group(30 patients)randomly. The control group was treated with flunarizine, and the treatment group was additionally treated with cervical traction and manipulation on the basis of the control group. After 4 therapeutic causes, evaluation of cervical vertigo symptoms and functions(ESOV)were performed and hemorheological changes were observed in both groups.Results① ESOV comparison: ESOV sores in both groupswere obviously improved after the treatment than the same group before the treatment(t=11.028, 5.825,all P<0.01)the treatment group were significantly lower than that in the control group after treatment(t=3.6057,P<0.01).② hemorheology changes comparison: whole blood viscosity(high-, medium- , low shear), whole blood reduction viscosity, erythrocyte aggregation index, hematocrit in the treatment group were improved obviously after the treatment than before(t=3.356, 13.245, 3.650, 7.443, 9.912, 3.113,P<0.05 orP<0.01). The whole blood viscosity(high-, medium-shea), blood reduction viscosity, erythrocyte aggregation index in the control group were obviously improved than the before(t=2.144, 4.216, 3.287, 3.039, 6.442,P<0.05 orP<0.01). The difference between the two groups were also obvious after the treatment(P<0.05 orP<0.01).Conclusions Cervical traction and manipulation combined with flunarizine can elevate ESOV score, improve hemorheology and therapeutic effect in patients with cervical vertigo.

This paper focuses on the requirements of joint operations under informationization in the future,and system-atically analyzes the characteristics of medical protection with regard to epidemic causes and surveillance,prevention meas-ures,mental health,and nuclear,chemical and biological protection.This paper elaborates on the basic principles of medical protection and raise proposals about health and epidemic protection so as to provide references for health support of PLA during joint operations.