Objective To investigate the relationship between the ultrasonic sign and expression of HMGB1 ,VEGF in serum of hepatocellular carcinoma patients .Methods Doppler ultrasound was used to inspect the size of the tumor and to analyze the typing and flow classification .The ELISA was used to check the HMGB1 and VEGF in serum .Results The expression level of HMGB1 in nodular and massive group was higher than that in small modelgroup (53 .5 ± 4 .3) ng/mL .The expression level of HMGB1 in massive group(102 .9 ± 9 .7)ng/mL was higher than that in nodular group(76 .0 ± 6 .2) ng/mL(P<0 .05) .The VEGF among three groups had no difference(P>0 .05) .With the increase of flow classification ,the levels of HMGB1 and VEGF also increased(P<0 .05) .Conclusion The HMGB1 in serum has some connection with morphology types .The HMGB1 and VEGF in serum increase with the flow classification .

Objective To explore the relationships of traumatic brain injury ( TBI ) and recognition and empathy.Methods 120 cases with moderate to-severe TBI and 120 healthy controls with age and gender-matched were chose.Participants were evaluated for facial and vocal affect recognition;and affective and cognitive empathy (empathic concern and perspective-taking,respectively) were detected.Results Participants with TBI had signifi-cantly poorer facial and vocal affect recognition.Facial and vocal affect recognition variances (14.28 ±3.87) points and DANVA2-Voice (14.12 ±3.14) points were significantly lower than those of the control group ( t=-3.927, 4.183,all P<0.05);the empathic concern score (19.58 ±4.87) points and perspective taking(14.46 ±5.34) points were significantly lower than those of the control group too (t=-3.015,-3.935,all P<0.05).Conclusion TBI patients have a tendency to avoid thinking about emotions and more likely to have problems recognizing others′emotions and assuming others′points of view.

Objective To study the diagnostic value of 3T three-dimensional contrast-enhanced MR angiography (3D CE-MRA) of arteriosclerosis occlusive disease of lower extremities using a low dose of gadolinium.Methods 26 patients with arteriosclerosis occlusive disease of lower extremities were prospectively enrolled.DSA and 3D CE-MRA with a low dose of gadolinium were per-formed and directly compared by radiologists.Sensitivity,specificity,accuracy,positive and negative predictive values,Kappa val-ue of 3D CE-MRA were determined for significant stenoses and vessel occlusions (5 1%-100%).Results The diagnosis coincidence rate of 3D CE-MRA was 87.21%,over-and underestimation rates were 8.91% and 3.88%,respectively.Sensitivity,specificity, accuracy,positive and negative predictive values,Kappa value of 3D CE-MRA in the characterization of significant stenoses and oc-clusions were 91.67%,94.29%,93.22%,91.67%,94.29%,0.860,respectively,in aortoiliac arteries,and 90.63%,94.23%, 92.86%,90.63%,94.23%,0.849,respectively,in femoropopliteal arteries and 93.67%,75%,87.83%,89.1 6%,84.38%, 0.718,respectively,in infrapopliteal arteies.The overall sensitivity,specificity,accuracy,positive and negative predictive values, Kappa value in the lower extremity were 92.59%,88.62%,90.70%,89.93%,84.50% and 0.813.Conclusion 3T 3D CE-MRA with a low dose of gadolinium is an accurate method used to depict significant stenoses and occlusions comparable with that of DSA in lower extremity artery disease.

ObjectiveTo observe the effect ofTong Guan Li Qiao(opening orifices) needling method on the swallowing function in patients with post-stroke deglutition disorders.MethodNinety-one patients were randomized into an acupuncture group of 46 cases and a control group of 45 cases. The acupuncture group was intervened byTong Guan Li Qiaoneedling method, while the control group was by rehabilitation training. The Standard Swallowing Assessment (SSA), Modified Barthel Index (MBI), Swallowing-related Quality of Life (SWAL-QOL) and Hamilton Depression Scale (HAMD) were adopted for evaluation before and after intervention.ResultAfter intervention, the SSA, MBI, SWAL-QOL, and HAMD scores were significantly improved in the two groups (P<0.05); there were significant differences in comparing the scores between the two groups after intervention (P<0.05), and the improvements in the acupuncture group were more significant than that in the control group (P<0.05).Conclusion Tong Guan Li Qiaoneedling method can produce a more significant efficacy than conventional rehabilitation, by more effectively improving the swallowing function, activities of daily life, quality of life, and depression state in patients with post-stroke deglutition disorders.

Objective:To observe the clinical efficacy of nape cluster acupuncture in treating migraine. Methods:Fifty patients with confirmed diagnosis of migraine were intervened by using nape cluster acupuncture, and were evaluated at the outset and after 2-month treatment by the short-form of McGill pain questionnaire (SP-MPQ) and self-rating depression scale (SDS). Results:After treatment, the number of positive words, sensory pain rating index (S-PRI), affective pain rating index (A-PRI), total pain rating index (T-PRI), visual analogue scale (VAS), and present pain intensity (PPI) were significantly changed (P<0.01); the SDS score was (56.42±8.12) points before treatment versus (41.08±5.73) points after treatment, and the difference was statistically significant (P<0.01). The total effective rate was 86.0%; the therapeutic efficacy of the patients with a shorter disease duration was superior to that of the patients with a longer one (P<0.05); the efficacy of mild-moderate migraine was superior to that of severe one (P<0.01); the total effective rate of patients without depression was higher than that with depression, but without a significant difference in comparing the therapeutic efficacy (P>0.05). Conclusion:Nape cluster acupuncture is effective in treating migraine, significantly improving headache and depression.

Objective To investigate the effect of applying the evidence-based nursing in elderly hypertensive cerebral hemorrhage patients with minimally invasive therapy.Methods 78 cases of hypertensive cerebral hemorrhage patients with minimally invasive treatment were recruited from May 2010 to May 2011.According to the conventional method of random numbers,they were divided into the evidencebased group and the conventional group with 39 cases in each group.The evidence-based group received evidence-based nursing,while the conventional group received conventional clinical nursing.The nursing effect of the two groups was observed.Results The satisfaction degree of the evidence-based group and conventional nursing group was respectively 94.9％ and 79.5 ％.There was statistically significant differences.The score of SDS,SAS and BI before treatment between two groups showed no significant difference,while it showed statistically significant differences after treatment between two groups.The complication rate of the evidence-based group and the conventional nursing group was respectively 10.2％ and 35.9％.There was statistically significant differences.Conclusions It is feasible to implement evidence-based nursing in the elderly hypertensive cerebral hemorrhage patients with minimally invasive therapy.It can effectively improve the patients' negative emotions,enhance daily living and quality of life.It is simple and can effectively reduce the incidence of complications.Thus,it is worthy of clinical application.

Objective To investigate the expression and significance of ERα (estrogen receptorα),ERβ(estrogen receptorβ),PR,and Bcl-2 of endometrial polyps.Methods 50 cases of endometrial polyp patients were studied.And 45 cases of endometrial normal subjects were selected as the control group.The enzyme-linked immunosorbent assay was used to detected the FSH,LH,E2,P,T and PRL six levels of the two groups.And endometrial ERα two groups of patients,ERβ,PR and Bcl-2 expression was studied.Results The FSH,LH,E2,P,T and PRL six levels of the two groups were no significant difference(P ＞0.05).Polyps in patients with proliferative phase of ERα,ERβ expression levels of the normal group had no significant difference (P ＞ 0.05),the expression level of PR(0.47 ± 0.02) point was significantly lower than that of the normal group(0.53 ± 0.02) point (t =10.383,P =0.000),Bcl-2 expression levels(0.53 ± 0.04) point was significantly higher than that of the normal group (0.49 ± 0.02) point (t =4.322,P =0.000).Polyps in patients with secretory phase of ERα,Bcl-2 expression levels (0.46 ± 0.07;0.43 ±0.04)point were significantly higher than that of the normal group (0.37 ± 0.05 ;0.39 ± 0.03) point (t =5.007,P =0.000; t =3.834,P =0.000),the expression level of PR (0.42 ± 0.02) point was significantly lower than that of the normal group (0.46 ± 0.01)point(t =8.486,P =0.000),there was no significant difference in expression levels between the two groups ERβ(P ＞0.05).Between the two groups displayed,ERα,PR proliferative phase of the normal group and the polyp group,the expression levels of Bcl-2 were significantly higher than that of the secretory phase (P ＜ 0.05).Conclusion The increase of the ERα,Bcl-2 expression levels,and the reduction of the level of PR expression in patients with endometrial polyps are closely related.

This paper took the charity culture cultivation system of the medical university as the research ob-ject, used the method of empirical research , illustrate how to build the charity culture educational system based on the practice of charitable culture education in Wenzhou medical university .The following:imparting charity culture knowledge , setting up the concept of charity;carrying out thematic education activity , cultivating gratitude;carry-ing out community volunteer service , creating the atmosphere of charity;creating charitable culture brand , building a charity culture education platform were discussed .Furthermore , the practice effect of the charity culture cultiva-tion system was also discussed from educating students , campus culture and promoting social morality .

Objective To investigate the effect of levocarnitine ane hemoperfusion on micro-inflammatory state of maintenance hemoeialysis patients. Methods Ninety-nine hemoeialysis patients were selectee as our subjects who hospitalizee from Jan. 2006 to Jan. 2013 in Provincial Hospital Affiliatee to Shaneong University ane were raneomly eivieee into A,B ane C groups. Thirty-three patients in A group were treatee with hemoperfusion combinee with L-carnitine therapy,33 cases in B group were appliee hemoperfusion ane 30 cases in C group were treatee with oreinary eialysis. The level of high sensitivity C reactionprotein( hs-CRP),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α)were measuree at before treatment,1ane 6 months after treatment. Results The levels of IL-6 in A group at before treatment,1 ane 6 months were(( 77. 53 ±12. 64),(62. 17 ± 11. 09),(42. 76 ± 10. 22))ng/L respectively,ane those were((76. 13 ± 13. 46), (70. 07 ± 2. 54),(59. 35 ± 11. 21))ng/L respectively in B group ane((78. 10 ± 10. 74),(72. 81 ± 11. 73), (68. 11 ± 13. 53))ng/L respectively in C group. The eifferences were significant( F within group=11. 642,P<0. 01;F among groups=6. 341,P<0. 01,F interaction=18. 275,P<0. 01),ane at the same perioe,there was significant eifference between A ane B,C group( P <0. 05 ). The levels of TNF-α in A group at before treatment,1 ane 6 months were((43. 59 ± 9. 81),(32. 34 ± 8. 68),(25. 7 ± 71. 27))ng/L respectively,ane those were((44. 53 ± 8. 62),(39. 15 ± 9. 72),(34. 82 ± 7. 21))ng/L respectively in B group ane((42. 19 ± 6. 72),(38. 63 ± 7. 65),(36. 98 ± 5. 89))ng/L respectively in C group. The eifferences among 3 groups were significant( F within groups = 10. 630,P < 0. 01;F among groups = 6. 654,P < 0. 01;F interaction=18. 273,P<0. 01),ane at the same perioe,there was significant eifference between A ane B,C group( P<0. 05). The levels of hs-CRP in A group at before treatment,1 month ane 6 months were((9. 34 ± 1. 16), (7. 28 ± 1. 05)ane(5. 13 ± 0. 84))mg/L. Those were((9. 05 ± 1. 36),(7. 97 ± 1. 08),(5. 82 ± 1. 13))mg/L in B group ane((9. 25 ± 1. 34),(8. 83 ± 1. 12),(8. 56 ± 1. 08))mg/L in C group. The eifferences among three groups were significant( F within group=12. 002,P<0. 01;F among groups=6. 681,P<0. 01;F interaction=17. 753,P<0. 01). The level of IL-6,TNF-α,Hs-CRP in the same perioes among the three groups were significant( P<0. 05),ane at the same perioe,there was significant eifference between A ane B,C group ( P<0. 05). Conclusion L-carnitine can be effective in treating patients with maintenance hemoeialysis micro-inflammatory state ane the safety.