Objective To investigate the state of self-perceived burden (SPB) and depression and to explore the relationship between them for patients with chronic respiratory diseases.Methods 95 patients with chronic respiratory diseases were investigated by the Self-Perceived Burden Scale (SPBS) and Self-Rating Depression Scale (SDS).Results The average score of SPBS was (33.64±10.23) points,which was at the middle level,90.5％ of patients showed different levels of burden,and 34.7％ of patients were at the high level.The average score of SDS was (50.23±11.93),the index was 0.63,which was at the middle to high level,74.7％ of patients showed different levels of depression,and 33.7％ of patients were at the high level.There was a positive conrelation between SPB and depression.Conclusions Patients with chronic respiratory diseases had obvious SPB and depression,and there was a close relationship between them.With chronic respiratory diseases breaking out repeatedly and more severe year by year,most patients have high level of burden and negative emotions.Nurses should take proper measures and psychological interventions to help patients face to the diseases optimistically,and to improve patients' emotion state and enhance their physical and mental health.

The paper discusses whether to maintain nonhealthy foetus,the bearing control of patients with hereditary diseases,the aim to apply the technique to distinguish foetus' sex and personal morals and social ethical problems for health staff to do that,points out that the technique can be applied only to check hereditary diseases as to be benefit to bearing good child and arising people's quality ,and the techique should be controlled properly as not to serve for feudal ethic.

Alzheimer Disease ; pathology ; Drugs, Chinese Herbal ; pharmacology ; Humans ; Rhodiola

With the wide application of Chinese herbal medicine, herb-drug interaction (HDI) has become increasingly prominent. Metabolic enzymes and transporters are the main targets of HDI, because the changes in expression and function of enzymes and transporters can influence the disposition of drugs. Metabolic enzymes are responsible for the metabolic clearance of drugs, including cytochrome P450 (CYP), UDP-glucuronyl transferase (UGT) and sulfotransferases (SULT); transporters widely expressed in the intestine, kidney, liver and brain are involved in the oral absorption, distribution and excretion of drugs. Pueraria, ginkgo, ginseng, St. John's wort and other Chinese herbal medicine often induce a HDI because those herbal medicines combined with chemical medicine are widely used in clinic. The components of herb medicines mentioned above are prone to interact with enzymes and transporters, which often induce a HDI. This paper reviews the advances in the study of enzymes and transporters-mediated pharmacokinetic mechanism of HDI.

Ovarian cancer is highly lethal despite multiple therapies, and new therapies are therefore needed.Retinoids are potential anticancer drugs that can promote apoptosis,inhibit growth and induce differentiation in cancer cells,and all these functions are regulated by the expression of retinoid receptors.Herein the recent researches on the expression of retinoid receptors in ovarian cancer are reviewed.

A large quantity of mice McAb ascites with monoclonal antibody (McAb) hybridoma cells separated from mice ascites was prepared. Ascites of mice inoculated with McAb hybridoma cells were collected, and the McAb hybridoma cells were isolated by centrifugation with lymphocyte separating fluid. McAb hybridoma cells then were injected into other mice abdominal cavity to prepare ascites again. McAb hybridoma cells separated from a mouse could be injected into the abdominal cavity of 5 mice.　Each mouse could produce 3.97 ml ascites on average. This method could be used to prepare large quantity of high titer mice monoclonal anti-HBs/a ascites.

Cytochrome P450(CYP450)is the most important family of enzymes in microsomal mixedfunction oxidase,widely distributed in vivo.CYP450 is involved in the metabolism of many exogenous compounds,the generation of endogenous substances,in particularly,affecting the occurrence and development of the tumors and their drug treatment,in the fields of medical and pharmaceutical research,cytochrome P450 has been very noticeable.

Australia is a country with strict drug administration, which is hard for its market access. Chinese Medicine Registration Act 2000 enacted by Victoria State of Australia established the legal status of traditional Chinese medicine in Victoria State, which made Australia the first one of western nations to legislate traditional Chinese medicine. We should take advantage of this opportunity to give in-depth analysis of laws and regulations on traditional Chinese medicine in Australia to push on traditional Chinese medicine to enter the Australia market. By analyzing the regulations of traditional Chinese medicine in Australia market, we put forward the following strategies on traditional Chinese medicine accessing Australia market: first, we should strengthen cooperation of Chinese and Australian governments; secondly, we should focus on analysis of regulations of drugs of Australia, thirdly, we should establish technical standard of herbal drugs in conformity with international standard; finally, we should strengthen traditional Chinese medicine culture exchange.

MicroRNA(miR)-203 is a stemness-inhibiting miRNA that adjusts the epithelioid cell differentiation by restricting the expression of stemness-related transcription factor.Its abnormal expression has been detected in several types of human cancers,including bladder cancer,breast cancer,colon cancer and pancreatic cancer.miR-203 plays a critical role in the tumor genesis and development by regulating cell proliferation,differentiation and apoptosis.

BACKGROUND:Because of deficiency of nerves and blood vessels of articular cartilage, its nutrition is mainly derived from the synovial fluid or synovial vascular osmosis. Limited in their ability to repair itself, so how to repair articular cartilage damage better become medical problems to be solved. OBJECTIVE:To review to the literatures on the repair of cartilage damage method and al ogeneic cartilage in vitro preservation method in recent years, and to find the optimal preservation conditions and the culture medium composition that suitable for in vitro preservation of al ogeneic cartilage tissues, thus enhancing the effect of al ogenic cartilage in vitro preservation. METHODS:A computer-based online search was performed in the PubMed database and the CNKI database to search papers on the in vitro liquid preservation of al ogeneic cartilage graft published from January 1990 to February 2013. The key words were“osteochondral al ograft, tissue culture, chondrocyte survival rate, in vitro”in English and“articular cartilage;al ogeneic transplantation;liquid preservation”in Chinese. The articles published earlier and repetitive researches were excluded. RESULTS AND CONCLUSION:At present, there are two methods for the preservation of al ogenic osteochondral grafts. After cryopreservation, the survival rate of chondrocytes is decreased significantly which can affect the transplantation effect, therefore there is less clinical application. Liquid preservation method can enhance the survival rate of cells, maintain the organization activity, but the preservation time is short that cannot be widely used. Scholars have further improved the environment of liquid preservation and composition of liquid culture medium, extended the in vitro preservation time of cartilage tissue, and improved the effectiveness of cartilage tissue preservation.