The present paper reports a determination of copper, zinc and aluminium in caudate nuclei in 8 adult cadavers and in 15 newborn cadavers by atomic absorption spectrophotometry using the graphite furnace atomizer. The results showed that the normal content of copper in the adult caudate nucleus was 24.04?8.93mg/kg dry tissue, zinc 129.92+89.17mg/kg and aluminium 2.38?0.77mg/kg. In the newborn cadavers, the normal contents of copper zinc and aluminium were 11.00+5.49, 75.35+27.52 and 1.72?0.56mg/kg dry tissue respectively. These results indicate that the data Iisted above in adults are higher than in newborn in fants.

Object To survey the worldwide medicinal plant resources of Illicium L Methods On the basis of herbarium, we studied on more than 10 000 speciemens of Illicium L from 121 Herbaria in 18 countries and the reviewed literature Results Fifteen species in Illicium L in the world belong to the medicinal plants, and they distribute in China, India, Burma and Vietnam In addition, the province scale distribution, habitat preference, and medicinal value are provided for each of the 15 medicinal plants Conclusion The medicinal plant resources of Illicium L from China are the most abundant in the world, and should be rationally exploited and widely utilized

Liver metastasis is the leading cause of death from colorectal cancer.Surgical treatment is so far the best choice for the treatment of colorectal liver metastases (CRLM).However,how to suitably select an individualized chemotherapy in perioperative period and surgical time and tool is still under controversy.Therefore,the key factors of improving the prognosis of patients with CRLM include overall evaluation of the patients and their preoperative examination outcomes,dividing patients into different groups based on different therapeutic goals,understanding different therapeutic goals of each group,selecting suitable chemotherapy and targeted therapy,ensuring surgical time (synchronous or stepwise resection) and tools (minimally invasive or open surgery) through multidisciplinary team model.

Objective To analyze the correlation of serum hs‐CRP and IL‐6 levels with cerebral hemorrhage volume and nerve function damage .Methods One hundred and two cerebral hemor‐rhage patients served as an experimental group and 64 individuals undergoing physical examina‐tion served as a control group in this study .The patients in experimental group were further di‐vided into mild damage group (n= 29) ,moderate damage group (n= 42) ,severe damage group (n=31) ,10 ml hemorrhage group(n=15) ,10 -30 ml hemorrhage group (n=52) ,and >30 ml hemorrhage group (n=35) .Their serum hs‐CRP and IL‐6 levels were measured on days 1 ,5 ,10 and 15 after admission .Results The serum hs‐CRP and IL‐6 levels in experimental group in‐creased gradually in the first 5 days ,reached their peak on day 5 and then decreased gradually (P<0 .05) .The serum hs‐CRP and IL‐6 levels were significantly higher in severe damage group than in mild and moderate damage groups ( P<0 .05) ,and in experimental group than in control group (9 .46 ± 1 .26 mg/L vs 4 .24 ± 0 .94 mg/L ,P=0 .02) .Conclusion The serum hs‐CRP and IL‐6 levels are related with cerebral hemorrhage volume and nerve function damage ,and can thus be considered as an important parameter in clinical testing .

BACKGROUND: Although clinical methods for autologous peripheral blood stem cell separation and identification arerelatively more, there is no unified standard. Moreover, little is reported on immune effects on lung adenocarcinomaduring autologous peripheral blood stem cell transplantation.OBJECTIVE: To study the effect of autologous peripheral blood stem cell transplantation on immunological function oflung adenocarcinoma rats by exploring the separation and identification methods of autologous peripheral blood stemcells.METHODS: The mononuclear cells were isolated from the peripheral blood of Wistar rats by density gradientcentrifugation method and then identified. Eighteen Wistar rats were randomly divided into routine treatment group andcell transplantation group, with nine rats in each group. Animal models of lung adenocarcinoma were established in therats, and underwent no treatment in the routine treatment group but injection of autologous peripheral blood stem cellsuspension (0.2 mL, 2.5×106/L) via the tail vein in the cell transplantation group. Immune indicators were detected in twogroups after treatment. Three rats from each group were anesthetized and scarified at 4, 10, 14 days after treatment,respectively. Afterwards, we detected the effects of autologous peripheral blood stem cell transplantation onimmunological function of lung adenocarcinoma rats.RESULTS AND CONCLUSION: Newly isolated mononuclear cells from rat peripheral blood showed a round or ovalshape and were relatively small in size. After 5 days of culture, autologous peripheral blood stem cells grew rapidly, andthe round cells began to spread to both ends and became spindle-shaped, thereby forming small colonies. Autologousperipheral blood stem cells induced in vitro experienced three stages successively: cell arrest, logarithmic and plateauphases. Flow cytometry showed that sorted cells were identified as autologous peripheral blood stem cells by expressingCD90, CD133 and Flk-1. After 4, 10 and 14 days culture, the expression of CD133 increased first and then descended,and Flk-1 showed an increasing trend. Compared with the routine treatment group, the expression of tumor necrosisfactor-α induced protein 6 in the cell transplantation group was significantly higher (P < 0.05), while the expression ofnuclear factor κB was significantly lower (P < 0.05). In conclusion, the isolation and identification methods of autologousperipheral blood stem cells are simple and can be used to secrete a large number of nutritional factors in the treatment oflung adenocarcinoma, which can enhance body immunity in the treatment.

Autophagy is a physiological process which delivers the mutant cytoplasmic proteins and dysfunctional subcellular organs into lysosomes for degradation to generate fuel in the deficiency conditions. It is mainly classified into macroautophagy, microautophagy and chaperon-mediated autophagy (CMA), as well as the selective autophagy such as mitophagy and aggrephagy. This review mainly introduces the key molecular markers of macroautophagy, CMA and mitophagy.

DNA methylation is one of the regulators on the expression of genes,and the expression of genes can be ”silenced” by hypermethylation of the tumor suppressor genes,which is in close relationship with carcinogenesis.Gastric cancer is caused by multiple factors and multiple genes alteration and prolonged interaction.among them ,There is considerable relation between the hypermethylation of the tumor suppressor genes and the gastric carcinogenesis.

As antigen-presenting cells, DCs are the most potent and unique,they can stimulate the primitive T cells. They play a major role in initiating the immune response. Tumor infiltrating dendritic cells (TIDCs) can reflect the antitumor immune capability of the host. The density of TIDC perhaps is associated with the prognosis in some cancers. The disablement of TIDCS is partly due to the phenotype they have. Perhaps it is the immunosuppressive factors secreted by tumor cells that have changed their phenotype.The studies about TIDCs provide the basis for DC-mediated antitumor immune therapy,and suggest the use of DC to treat tumors through different approaches.

Objective To explore the relationship betw een injury age and expressions of erythropoietin (EPO ) and its receptor EPO R in the brain tissue of rats after cerebral injury. Methods Seventy-tw o rats w ere random ly divided into control group (36 rats) and cerebral injury group (36 rats). The rats w ere sac-rificed at 1, 2, 4, 8, 12, 24 h after cerebral injury (6 rats at each tim e point) and the brain tissues w ere extracted. The expressions of m RNA and protein of EPO and EPO R at different tim e points w ere de-tected by real-tim e fluorescent quantitative PC R and W estern bloting. Results The expressions of EPO and EPO R increased w ithin 24 h after injury. The expressions of m RNA and protein of EPO w ere relat-ed to the injury age, and the correlations w ere 0.875, 0.911, respectively (P<0.05). The expressions of m RNA and protein of EPO R w ere related to the injury age, and the correlation coefficients w ere 0.936, 0.905, respectively (P<0.05). Conclusion The expressions of EPO and EPO R increase gradually in the early stage of the rat’s cerebral injury, w hich are associated w ith the injury age and could be a useful value for estim ating injury age.

Objective To explore the clinic effect of marvelon and estrogen on puberty dysfunctional uterine bleeding.Methods 50 patients were evaluated inclouding 20 patients treated by estrogen in estrogen(E) group and 30 patients treated by marvelon in progestin and estrogen(PE) group.Results The time when bleeding was stoped completely were (21.65?9.62) hours in E group and (13.68?5.67) hours in PE group(P