OBJECTIVETo observe differences of clinical therapeutic effects between deep-oblique acupuncture with long needle and regular perpendicular acupuncture at Guanyuan (CV 4) for urinary retention to explore standard acupuncture method in clinic.

METHODSOne hundred and thirty-two cases of urinary retention were randomly divided into an observation group (78 cases) and a control group (54 cases). Needles with 75 mm in length were deeply and obliquely inserted into Guanyuan (CV 4) in the observation group while needles with 40 mm in length were perpendicularly inserted. Clinical therapeutic effects in two groups were observed.

RESULTSAfter treatment the total effective rate was 80.8% (63/78) in the observation group and 57.4% (31/54) in the control group, which had significant difference between two groups (P<0.05). The curative effect of postpartum retention of urine was obviously superior to that of postoperative and prostate-induced urinary retention in the ebservation group, and the effect of urinary retention due to different etiology in the observation was also superior to that in the control group in the ebservation group [in the observation group total effective rate was 88.6% (47/53) in postpartum retention of urine, 50.0% (3/6) in prostate-induced urinary retention and 68.4% (13/19) in orthopedic postoperative retention of urine while in the control group total effective rate was 69.4% (25/36) in postpartum retention of urine, 20.0% (1/5) in prostate-induced urinary retention and 38.5% (5/13) in orthopedic postoperative retention of urine)].

CONCLUSIONThe deep-oblique acupuncture with long needle at Guanyuan (CV 4) has significant curative effect on urinary retention in clinic, especially for patients with postpartum urinary retention, which could be taken as standard acupuncture method.

Acupuncture Points ; Acupuncture Therapy ; Adult ; Aged ; Female ; Humans ; Male ; Middle Aged ; Treatment Outcome ; Urinary Retention ; therapy

Antirheumatic Agents ; therapeutic use ; Arthritis, Rheumatoid ; drug therapy ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Etanercept ; Female ; Humans ; Immunoglobulin G ; therapeutic use ; Male ; Methotrexate ; therapeutic use ; Phytotherapy ; Prednisone ; therapeutic use ; Receptors, Tumor Necrosis Factor ; therapeutic use

Objective To observe the therapeutic efficacy of centro-square needling plus stuck needling with lifting-thrusting manipulations in treating the injury of medial collateral ligament (IMCL) of knee joint. Methods Seventy-eight patients with IMCL of knee joint were randomized into a treatment group of 40 cases and a control group of 38 cases. The treatment group was intervened by centro-square needling plus stuck needling with lifting-thrusting manipulations, while the control group was by local anesthesia treatment. The therapeutic efficacies were compared between the two groups. Results The total effective rate was 97.5% in the treatment group versus 81.6% in the control group, and the difference was statistically significant (P＜0.05). Conclusion Centro-square needling plus stuck needling with lifting-thrusting manipulations is an effective way in treating IMCL of knee joint.

Objective:Explore decoy oligonucleotide (ODN) technology targeted blocking STAT3 activation way effects on colorectal cancer HT29 cell growth,to provide experimemt basis for gene therapy of colorectal cancer.Methods:Using decoy ODN in vitro transfect colorectal cancer HT29 cell line,to targeted blocking STAT3 activation way.Fluorescence microscope and flow cytometry detect transfection conditions and efficiency,and MTT method detect the cell growth inhibition,Realtime RT-PCR and Western blotting test STAT3 in cells,the Bcl-xl and Caspase3 mRNA and protein expression.Results:STAT3 decoy ODN can efficient transfection into colorectal cancer cells,and is due to the nucleus.HT29 cell in the STAT3 decoy ODN,growth inhibition rate increased significantly,and mRNA and quantity of protein expression of STAT3 significantly reduce (P＜0.05),while caspase3 increase significantly (P＜0.05).Conclusion:Named Decoy ODN targeted after blocking STAT3 activation way,which can effectively cut colorectal cancer cells HT29 STAT3 gene expression,and inhibit the growth of the cells.The mechanism may be related to lower the expression of STAT3 and the Bcl-xl and Caspase3.

Corneal collagen cross-linking (CXL) is a photochemotherapy for keratoconus, keratectasias and infectious keratitis.Corneal collagen fiber infiltrated with riboflavin, a photosensitizer which generates reactive oxygen species when activated by UVA at 370nm, was induced to form the crosslinks in corneal stroma.Corneal collagen cross-linking alone or combined with medicine therapy could be used to treat fungal keratitis, improve the cure rates, and reduce the complications and the demands of corneal transplantation surgery.The purpose of this paper is to review the basic principle, procedure, laboratory researches, clinical applications and the safety about this treatment.

Objective To evaluate the investigation outcome of eliminating iodine deficiency disorders(IDD)in Ya'an city of Sichuan province,and to master the current situation of IDD in the whole city.Methods In 2009,according toCounties'Assessment Guideline in Sichuan for Implementing the Measure to Eliminate Iodine Deficiency Disorders,assessments were conducted.At county level,composite score was given to collected data of the eight counties(districts)and monitoring data of household iodized salt were statistically analyzed.A township (town)was randomly selected by east,west,south,north and center locations,respectively.One primary school was randomly selected in each selected township(town),eighty children aged 8 to 10 in each selected school were checked of their thyroid by palpation(WS 276-2007)and urinary iodine level of20 children among them was measured by As3+_Ce4+ catalytic spectrophotometry(WS/T 107-2006).Thirty fifth-grade students and 10 housewives lived around school were asked to take a questionnaire survey.Results Average score of the 8 counties(districts)was 89.96,ranged from 85.42 to 92.90.The coverage rate of iodized salt of household was 100.00%(2328/2328),rate of qualified iodized salt was 97.98%(2281/2328).and consumption rate of qualified iodized salt reached 97.98%(2281/2328).The goiter rate of children aged 8-10 was 0.91%(33/3620),median urinary iodine was 235.85μg/L,and 1.50%(12/800)of child's urinary iodine was less than 50μg/L.The rate of passing a knowledge questionnaire survey of the fifth-grade students and women was 92.33%(1 131/1225)and 9413%(397/421),respectively.Conclusions Ya'an city in Sichuan province has reached the goal of eliminating iodine deficiency.disorders,but there is a coexist trend of IDD and iodine excess.They should pay attention to the daily consumption of iodized salt in the future;sustained elimination of IDD can be reached only by iodine supplementation scientifically.

Objective To compare 2 methods to immobilize probes on the surface of gold membrane and select the better one for aptamer immobilization of aptamer-based piezoelectric quartz crystal sensor. Methods Anti-IgE aptamers were immobilized on the gold surface of piezoelectric quartz crystal sensor with thiol method or biotin-avidin method. The changes of frequency were compared in reactions with different concentrations of IgE. Results Biotin-avidin method obtained higher frequency changes in the reactions of IgE, lower limit of detection and wider liner range than thiol method. For biotin-avidin method, there was significant linear correlation between frequency changes and the concentration of IgE with the correlation coefficient of 0.994 5. Conclusion The thiol method which is proved effective for probe immobilization on gene sensor is unfit for aptamer immobilization. Biotin-avidin method has a better ability to immobilize probe on the surfaces of aptamer-based piezoelectric quartz crystal sensor.

Objective To examine the epidemiological and clinical features of the adults with Henoch Schonlein Purpura (HSP),and improve the diagnosis and therapeutic efficacy of HSP.Methods Thirty five adults with HSP from the hematological department of Tianjin Medical University General Hospital between 1993.9 to 2005.12 were enrolled in this study.Epidemiological,clinical and laboratory data,therapeutic methods and efficacy were investigated.Results Most patients with HSP in our department were male and adolescent.HSP occurred more commonly in the winter.The important predisposing factors were infection and having specific food.Purpuric lesions were the most common presenting signs.Multiple organ involvement was more common.Purpura was present in all case,lower extremities were more commonly involved.Joint involvement was observed in 15 cases(42.9%),abdominal involvement in 20 cases(57.1%),renal involvement in 20 cases(57.1%).Conclusion The adult patients with HSP had more tendenecy to have severe renal damage and multiple organ involvement.

Objective To observe the effect of antitumor and hematopoiesis of the extract of Spatholobus suberctus Dunn (SSCE) in vivo. Methods The mouse model of Lewis lung carcinoma was used to investigate the effects of SSCE on tumor growth and hematopoiesis by detecting the counts of peripheral blood, the counts and classify of the cells in bone marrow. Result The tumor inhibitory rate of SSCE on Lewis lung cancer was 30.65%. SSCE can stimulate the proliferation of bone marrow cells and relieve the marrow depression induced by chemotherapy, at the same time, the physique of the mouse treated by SSCE was not effected. Conclusion SSCE exits antitumor effect, moreover, it can stimulate the bone marrow cells to proliferate and relieve the marrow depression produced by chemotherapy.

Objective To discuss the distribution of coagulation markers in patients with advanced non-small cell lung cancer (NSCLC) and the correlation of these markers with the prognosis of the patients, the TCM syndrome and the long-term thrombotic events. Method Hospitalized NSCLC patients were selected for retrospective investigation and the coagulation markers data were analyzed with SPSS13.0. Result There were 10 cases (38.5%) and eight cases (30.8%) with one or more abnormal coagulation markers in the non-advanced NSCLC group, while there were 95 cases (71.4%) and 77 cases (57.9%) in the advanced NSCLC group. There were significant difference between the two groups (P