The purification system described in this paper includes two steps. The first step may be called "rough purification" by using potassium thiocyanate (KCNS) -alcohol fractionation precipitation method. The second step is "fine purification" by using Blue Dextran-sepharose 4B (BDS) column affinity chromatography methodThe experimental results showed that the optimum condition for precipitating the interferon (IFN) from the crude IFN solution could be made by adding 0.5M KCNS, and then adjusting pH to 4.0. The optimum pH values in separating out the IFN from acid alcohol-interferon solution were 5.5 to 7.2. The results between the salting out once and twice by KCNS were not significantly different. Centrifugation with centrifuge maintaining lower temperature with cold alcohol could be used instead of refrigerated centrifuge during purification. The IFN can be purified to about 106 international units per mg of protein with about 70% recovery on the first step of purification. In the IFN yielded on the second step of purification, the maximum specific activity of IFN in the collecting fractions was increased up to about 107 international units per mg of protein with a recovery rate of over 40%.The present experiments may offer the possibility in purifying the IFN for clinical use. The Suggested purification method will also render some valuable reference to purify other types of IFN.

Objective To observe the clinical efficacy of acyclovir combined with prednisone in the treatment of herpes zoster.Methods 128 patients with herpes zoster were selected as study objects,and they were divided into the treatment group and the control group by using the method of complete random distribution,64 cases in each group.The patients in the treatment group were treated with oral acyclovir and prednisone,while those in the control group received oral acyclovir alone.The clinical efficacy of the two groups was observed.Results The blistering,crusting and healing time had no significant differences between the two groups(t=1.76,1.79,1.41,all P＞0.05).The analgesic time of the treatment group was (3.2±1.2)d,which was significantly shorter than (7.4±2.6)d of the control group (t=11.73,P＜0.01).The total effective rate of the treatment group was 95.3％,which was significantly higher than 73.4％ of the control group,the difference was significant(χ2=16.13,P＜0.01).In the treatment group,there were 2 cases of postherpetic neuralgia.In the control group,8 cases had postherpetic neuralgia.The incidence rate of adverse events in the treatment group was significantly lower than that in the control group(χ2=6.01,P＜0.05).Conclusion Acyclovir combined with prednisone in the treatment of herpes zoster has significant clinical efficacy,it can shorten the analgesic time,and is worthy of clinical application.

Objective To explore the efficient method in detection of DMD/BMD patients.Methods 18 deletion-prone exon fragments of DMD gene were amplified via molecular cloning. They were used as probes and were spotted on the slides treated with APES and poly-lysine together by manual operation to make microarray. In addition, fragments of ?-actin were used as positive contrast and those of pUC 19/EcoR I were used as negative. 30 DMD/BMD patients were detected for deletion in DMD gene with the microarray and 5 healthy people were done as normal control. Parts of the results were compared with PCR method.Results Different exon fragment deletion of DMD/BMD gene was detected in 21 patients by DNA microarray, and 10 of them were confirmed by PCR analysis.Conclusion DNA microarray assay is a convenient ,accurate and sensitive method in diagnosis of DMD/BMD patient.

Objective To establish and clinically evaluate a method to adjust tube current(mA)individually for obtaining images with consistent noise and optimized radiation dose in 64-slice spiral helical CT coronary angiography.Methods (1)80 patients,as a control group,were underwent CT coronary angiography(CTCA)using 64-muhislice spiral helical CT with fixed mA(650 mA).Two radiologists first assessed image quality of the fixed mA group by scoring from 1 to 5.A minimal dose requirement for meeting clinical diagnosis with the desired noise level(standard deviation SD0)were decided.By analyzing the images of the control group,we also established correlation between the image noises of timing bolus(TB)scans and cardiac(CA)scans and set up a formula to predict the required mA value for obtaining the desired cardiac image noise for individual patient.(2)Subsequently,another group of 80 patients(study group)wero scanned with individually adjusted mA using the established formula and the practicability of this method was then evaluated.The independent sample t test was used to compare the values of the two groups.Results (1)The noise of TB and CTCA scans was linearly correlated with r2=0.994. (2)A cardiac image noise level of 28 HU was found to be adequate for clinical diagnostic purpose based on the image quality analysis.(3)The average noise for the acquired cardiac images was found to be 28.97 HU for the study group with individually adapted mA using the established formula.The standard deviation of the image noisein study group was 1.93,which was much smaller than 5.14 in the fixed mA group,but the average image scores for the study group(4.27±0.68)and fixed group mA(4.03 4-0.93)were not statistically different.(4)In the study group,63 out of 80 patients required tube currents below the standard 650 mA,yielding an average CTDIvol of58.32 mGy,which was about 32.1%dose reduction than that of fixed mA(85.94 mGy).The lowest CTDIvol was 19.27 mGy with 160 mA which was 22.4%of the average CTDIvol in control group.Conclusion Adjusting mA individually based on timing bolus scan noise analysis is an accurate and practical method to obtain consistent image quality for different cardiac pailents and to minimize the radiation dose for individuals compared to the fixed mA.

OBJECTIVE:To study the mechanism by which L-arginine regulates hypoxic pulmonary vascular structural remodeling.METHODS:Eighteen Wistar rats were randomly divided into control group,hypoxic group and hypoxic+L-arginine group.Pulmonary artery pressure was measured with right cardiac catheterization.Micro-structure and ultra-structure of pulmonary tissue were observed and collagen I expression was evaluated with immunohistochemistry.RESULTS:Mean pulmonary artery pressure(mPAP) was(2.7?0.3)kPa in hypoxic rats and(2.1?0.1)kPa in control rats(P

Objective To study the value of ultrasonographic diagnosis of benign,borderline and malignant ovarian epithelial tumors.Methods In the 203 patients of ovarian epithelial tumors,the substantial tissues′ area of 128 cases with cyst-solid mass was measured through ultrasonography and compared with pathology.Results The area of substantial tissues in ovarian epithelial tumors was benign 5 cm 2 were regarded as optimal threshold values for differentiation of the benign,the borderline and the malignancy,their sensitivities were 94.7% , 80.0% and 90.7% ,their specificities were 96.3% , 92.4% and 97.6% ,accuracies were 96.1% , 90.0% and 92.9% .Conclusions It provides an effective method for diagnosis of benign, borderline and malignant ovarian epithelial tumors.If combined with conditions of color Doppler flow imaging, and comprehensive consideration on greater omentum and lymph node, the accurate diagnostic rate will be increased.

Objective To investigate the function and mechanism of embryonic stem cells against Lewis non-small cell lung cancer in vivo. Methods Based on the mouse Lewis non-small cell lung cancer model, we have tested some tumor growth indexes and investigated the immune response of embryonic stem cells against cancer cells. Results Compared with the mice in control group, mice in experimental group received obvious antitumor immunity, which means more activated lymphocytes and antitumor cytokines, resulted in the effective control and inhibition of tumor development. Conclusion Besides the antitumor effect in vitro, embryonic stem cells can also generates immune response in vivo, which could effectively inhibit and/or delay the development of cancer.

Dysphasia is a fatal complication of stroke, and may result in other complications. Transcranial direct current stimulation (tDCS) is helpful for the recovery of dysphasia associated with stroke, which may involve in the polarity and cerebral plasticity. There are advantages and disadvantages of tDCS compared with repetitive transcrnial magnetic stimulation.

One hepatitis C virus(HCV) cDNA fragment, 534bp in length and designated as Q534, was obtained by means of PCR amplification with self-designed oligonucleotide primers. Q534 was doned into Hinc II site of pUC18 and the- recombinant plasmid pQ534 was then selected from the bacterial transformants. The sequence data indicated the Q534 was a cDNA fragment of HCV core gene, and located in HCV genome from positions 320 to 853 in correspondence with Chiron's prototype sequence. The homologies between Q534 and the prototype at the levels of nucleotides and amino adds were 90.9% and 97.6%, respectively. The homologies of Q534 with Japanese HCV-J and HCV-BK strains were 96.8% and 97.0% at the nucleotide level, and 98.2% and 98.8% at the amino add level. Compared with the corresponding sequences of other HCV isolates, this Chinese HCV isolate we obtained in the present study belongs to HCV group II .