Tissue engineering provides urologists a new way to fix or reconstruct the impaired organs.Reconstitution of corporal bodies of penis with engineered tissue substitutes has been applied in animal models.In hypospadias reconstruction,the use of engineered tissue substitutes has been applied clinically.The clinical application of bladder tissue substitutes has been ongoing phase II clinical trial.Great progress has been made in renal replacement therapy with clinical application of human progenitor cells in hemofiltration units,and the engineered intracorporeal renal replacement unit will come true by additional studies.The current status of tissue engineering in clinical practice of urology is reviewed in this paper.

Objective To evaluate the curative effects and side effects of burcea javanica oil emulsion(BJOE) in the treatment of pleural effusion of lung cancer by thoracic injection.Methods 40 cases with pleural effusion of lung cancer were treated by using BJOE.A dose of 50～100ml BJOE was injected intrapleurally,once every 5～7 days,4 time for a course of treatment,then the effects and side effects were observed after one month.Results The effective rate was 90%(36/40).The side effects were fever(n=4) and digestive sickness(n=2) as well thoracalgia(n=1).Conclusion Brucea javanica oil emulsion has good curative effect and low side effect in the treatment of pleural effusion of lung cancer.

Alveolar Bone Loss ; prevention & control ; Alveolar Process ; surgery ; Bone Regeneration ; Dental Implantation, Endosseous ; Dental Prosthesis, Implant-Supported ; Guided Tissue Regeneration, Periodontal ; methods ; Humans ; Immediate Dental Implant Loading ; Male ; Middle Aged ; Periodontitis ; surgery ; Tooth Extraction ; adverse effects ; Wound Healing

Objective To study the clinical features of primary signet ring cell carcinoma of prostate. Methods 2 cases of primary signet ring cell carcinoma of the prostate were studied and reviewed. Results The age of the 2 patients was 64 and 73.The clinical symptoms were dysuria,vesical irritability and perineum discomfort. Histologically, signet ring cell carcinoma was composed of round cells with abundant-clear cytoplasm and crescent-shaped nuclei on one side.Mitosis were frequently observed.Immunohistochemical testing showed the cancer cell was positive for prostate specific antigen (PSA),prostate acid phosphatase (PAP),AR,cytokeratin and negative for carcinoembryonic antigen (CEA),alcian blue/periodic acid-schiff (AB/PAS).One case (stage D)died 6 months after bilateral orchiectomy and flutamide therapy because of wide-spead metastasis;the other (stage B 2) has been surviving 25 months after radical prostatectomy,bilateral orchiectomy,endocrine therapy and local irradiation ministration. Conclusions Primary signet ring cell carcinoma of the prostate is extremely rare with a high grade of malignancy. The diagnosis depends on histopathologic and immunohistochemical studies.

Objective To explore the effect of incision of the cervix renal calices for the treatment of complex intrarenal sinus renal calculi. Methods 52 cases of complex intrarenal sinus renal calculi with upper and/or lower calyx serious hydrops treated by incision of the cervix renal calices were studied.There were 38 men and 14 women.The mean age was 43 years (range,21 to 70 years).The mean diameter of the stones was 4.2 cm. Results The stones were totally removed in 44 cases.8 cases with residual stones were treated by percutaneous nephrolithotomy. The residual stones were totally removed in 4 cases while other 4 cases accepted further treatment with extracorporeal shock wave lithotripsy (ESWL).No blood transfusion and postoperative bleeding were observed.24 cases were followed up of 0.5 to 4 years.KUB plus IVU were conducted postoperatively which showed improved hydronephrosis.No intrarenal stricture and renal calculi recurrence were observed. Conclusions The procedure has advantages of less bleeding,preservating more renal function,less postoperative complications,which has a good application in the treatment of complex intrarenal sinus renal calculi with upper and/or lower calyx serious hydrops.

Accidents, Occupational ; prevention & control ; Humans ; Occupational Diseases ; diagnosis ; Occupational Health Services ; organization & administration

Aged ; Anthracosis ; genetics ; Case-Control Studies ; Humans ; Male ; Middle Aged ; Polymorphism, Genetic ; Transforming Growth Factor beta ; genetics ; Tumor Necrosis Factor-alpha ; genetics

More and more attention has been drawn on nano-materials in the application of molecular imaging and targeted cancer therapy. Quantum dots, due to their rich surface chemical properties and bright stable fluorescence, have been used as a new type of nano-probes and widely used in molecular, cellular and vivo biological imaging. In recent years, many inspiring achievements have been obtained in the study on quantum dots surface modification and biological imaging in cell and animals. In this article, current status and the latest developments of the study and the application are reviewed.

Objective:To construct DNA vaccines expressing prostate-specific membrane antigen(PSMA) and/or granulocyte-macrophage colony-stimulating factor(GM-CSF) and to determine their immunoactivity.Methods: Recombinant plasmids pIRES-PSMA-mGM-CSF,pIRES-PSMA,and pIRES-mGM-CSF were constructed with DNA vaccine vector pIRES.After identified by endonuclease digestion,the above 3 plasmids and blank pIRES vector were used to immunize C56BL/6 mice(n=15).LDH release assay was used to exam the cytotoxicity of cytolytic T lymphocytes in each group.Results: We successfully constructed the above mentioned recombinant plasmids.Mice in pIRES-PSMA-mGM-CSF immunized group had the highest specific cytotoxicity,followed by pIRES-PSMA and pIRES-mGM-CSF immunized groups.The blank pIRES group had the lowest cytotoxicity(P

Objective To analyze the CT manifestations of mesenteric panniculitis,so that to improve CT diagnosis of mesenteric panniculitis.Methods Seven patients with mesenteric panniculitis,which were diagnosed according to the CT criteria,were analyzed.Results The fatty masses with defined margins and around the root of the jejunum were found in all patients at CT scan,and there were no enhancement after administration of contrast medium.The masses were sharply defined with abdominal and retroperitoneal fat.There were pseudo-capsule in 7, "arcus adiposus sign" in 5. Enlargement of lymph nodes in the masses and retroperitoneum in 2 cases were seen,respectively.4 patients had abdominal operations,one patient was in combination with carcinoma of head of pancrease.Conclusion Certain features of the CT manifestations of mesenteric panniculitis can be found,CT is effective for the diagnosis of mesenteric panniculitis.