Objective:To investigate whether placental tissues produce leptin and determine the relationship between expression levels of leptin-mRNA in placenta and fetal growth and development.Methods:52 neonates were divided into 3 subgroups:(1)birth weight large for gestational age (LGA)( n =14),(2)birth weight appropriate for gestational age (AGA)( n =21),(3)birth weight small for gestational age (SGA)( n =17).Reverse transcription-polymerase chain reaction(RT-PCR) technique was used to detect human leptin-mRNA derived from placental-RNA.The expression levels of leptin-mRNA were quantitated using fluorescence intensity analysis.The correlations of expression level of placental leptin-mRNA with placental weight,birth weight,newborn Ponderal index(PI),maternal weight,maternal body mass index(BMI) and gender were studied.Results:The expression levels of placental leptin-mRNA in LGA infants(119.7?13.0) and SGA infants(76.8?15.1) were significantly higher and lower respectively than those in the AGA infants(94.4?17.4)( P

Objective:To investigate the relationship between serum creatine kinase level and histopathological observation of tubal ectopic pregnancy.Methods:Serum creatine kinase was determined by N-acetyl cysteine(NAC) assay in 22 patients with ruptured tubal pregnancies and 35 patients with unruptured tubal pregnancies.Fallopian tube tissues were examined by histopathology.Results:The mean levels of serum creatine kinase in patients with ruptured tubal pregnancies were significantly higher than that in those with unruptured tubal pregnancies(154.2?63.7 IU/L vs 107.8?51.3 IU/L, P

Objective To study the expression of SH2-B? in the lung and visceral sensory afferent system(C-7-T-5 spinal ganglia and the corresponding posterior horn of the spinal cord) of the asthmatic mice,and to explore the role of SH2-B? in asthma.Methods Thirty BALB/c mice were randomly divided into normal control group and asthmatic group on average.The alterations of SH2-B? and nerve growth factor(NGF)immunoreactivity were investigated by immunohistochemical and Western blotting method and analyzed by the Metamoph analysis system.Results Immunohistochemistry demonstrated that the mean optic density(MA)of SH2-B? positive substance in the lungs(0.806?0.023),C-7-T-5 spinal ganglia(0.766?0.014)and the corresponding posterior horn of the spinal cord(0.547?0.014)of the asthmatic mice was much higher than those(0.243?0.018,0.131?0.011,0.215?0.029) in normal control group respectively.Western blotting also demonstrated that SH2-B? and NGF expressions were more increased in the lungs and C-7-T-5 spinal cord of the asthmatic mice than those of normal control group.The mean ratio of SH2-B? MA to ?-actin MA in spinal cord and lung was 0.346?0.017 and 0.512?0.018 in normal control group,0.738?0.021 and 1.526?0.022 in asthmatic group.The mean ratio of NGF MA to ?-actin MA in spinal cord and lung was 0.357?0.021 and 0.734?0.013 in normal control group,1.445?0.015 and 1.265?0.018 in asthmatic group.A positive correlation was found between the levels of SH2-B? and NGF(r=0.651,P

Objective: To evaluate the microbial safety of human amniotic membrane(HAM) in preparation and preservation,and to establish effective methods of microbe control for HAM bank.Methods: Human amnions were collected from elective caesarean sections in 20 healthy women,and divided into groups based on different sterilization procedures and preservation methods.Bacteria,fungi and mycoplasmas were detected at 24h,1m,3m and 12m after preservation.Other amnions collected in eutocia from 5 healthy women were detected bacteria,fungi,lactobacilli and mycoplasmas.The series of serological determination for the parturients were followed up in 6 months.Results: Two HAM samples only soaked in NS were detected to be contaminated with E.coli and/or S.epidermidis,respectively from two caesarean sections.There is no microbe detected in all of the amnions from caesarean sections which were soaked in the antibiotics solution.One HAM sample from eutocia was yet detected to have lactobacilli after soak.The series of serological determination were all negative.Conclusion: During one year storage,HAM only from caesarean sections in healthy women,can successfully avoid contamination if soaked in the antibiotics solution then prepared and preserved with aseptic technique.

OBJECTIVE:To briefly analyze the fresh herb processing and application methods in Shengcao Yaoxing Beiyao, and to offer references for modern fresh herb application. METHODS:All 311 herbs stated in Shengcao Yaoxing Beiyao were stud-ied individually to record their processing and application methods from property and performance. RESULTS & CONCLUSIONS:19 fresh herb processing methods and 25 application methods were recorded in this book. The 19 fresh herb processing methods in-cluded juicing method,mud method,soaking method,water-boiling method,wine-boiling method,ten-steaming and nine-basking method,carbon method,burning-ash method,chewing method,mincing method,cooking method,and so on. The 25 application methods of fresh herbs included applying,scraping,painting,sticking,washing,spreading,immersing,bathing,ear-dropping, fuming,oral administration with wine,mouth-keeping,juice-drinking,boiling,water-boiling-drinking and tea-drinking,frying, decocting,toasting,steaming,blending,stewing,oral administration with tea,pastry,brewing,alleviating fishy smell,and so on. It is necessary to strengthen the origins research and re-research of herbs,and develop several empirical studies about the influ-ence for fresh herbs’effective ingredient quantitative change by their processing methods,and the influence for fresh herbs’phar-macology index by application methods,in order to form intellectual property.

Objective To explore the relationship between metabolic syndrome (MS) and obesity in obese children,and to explore the risk factors of MS.Methods The study included 56 obese children (26 boys and 30 girls) and 50 non-obese controls (25 boys and 25 girls).Anthropometry,fasting glucose,insulin concentrations,total cholesterol,high density lipoprotein (HDL),low density lipoprotein (LDL),triglycerides,systolic and diastolic blood pressure(BP) were measured in both of groups.Insulin resistance was determined by HOMA-IR.Finally subcutaneous and visceral fat thicknesses were measured by ultrasonographically.Results Obese children with metabolic syndrome had a high level of BMI,waist circumference,insulin levels,HOMA-IR,TG,blood pressure and visceral fat thickness than obese children without metabolic syndrome,but HDL-C was over than control group.The proportion of obese children with insulin ≥ 15 μU/ml,HOMA-IR ≥ 3.4 and TG ≥ 150mg/dl was significantly higher,while the proportion of HDL-C ＜ 40mg/dl was significantly lower than the control group.In logistic regression analysis showed that HOMA-IR ≥ 3.4,TG ≥ 150mg/dl and HDL-C ＜ 40mg/dl was strongly associated with the occurrence of MS and the risk was increased with the HOMA-IR score.The ORs of MS recurrence in obese children with HOMA-IR score between 3.4-4.9 was 3.8 compared with the reference group.That of HOMA-IR≥5 was 5.7,that of TG≥ 150mg/dl was 4.2,that of HDL-C ＜40mg/dl was 3.4 (P ＜0.05).Conclusion Children with HOMA-IR score between 3.4-4.9,HOMA-IR≥5,TG≥ 150mg/dl or HDL-C ＜ 40mg/dL have an significantly increased risk for metabolic syndrome.So for these obese children,we should advance screening of high-risk patients and intervene as early as possible to prevent the occurrence of metabolic syndrome and its complications.

Objective:Introduce yeast two-hybrid system RRS screening system through interaction between two known proteins,Pak and Chp,in order to apply this system on research of the interaction between other proteins.Methods:Select the yeast strain cdc25-2 for single clone that displays low reversion rate,and prepare the competences of the yeast by LiAc.Only transform the plasmid Met425-Myc-Ras-Pak into the yeast to eliminate its self-activation.Co-transform the plasmids Met425-Myc-Ras-Pak and Ura-M-ChpAc into the yeast,set up some controls,observe their growth on different mediums at 36℃.Results:The cdc25-2 cells which are used to prepare competences have no reversion.After the transformation of the plasmid Met425-Myc-Ras-Pak into the yeast,there is neither toxicity nor self-activation.Only when the two plasmids which can express the two corresponding known proteins are co-transformed,and only on the medium suitable for their expression,the cdc25-2 cells can grow at 36℃.Conclusion:(1)The RRS screening system verifies the interaction between Pak and Chp correctly.So it can be used for research of the interaction between other known proteins.(2)We can construct a bait of an known protein,then use the RRS screening system to look for the interacted proteins.

We reviewed the domestic studies on Scutellaria Baicalensis Georgi.Scutellaria Baiealensis Georgi has a variety of pharmacological effects,and the main effects are anti-microbial,anti-inflammatory and anti-tumor functions.Scutellaria Baicalensis Georgi has good developing prospects.Up to now,Chinese researches on Scuteilaria baicalensis Georgi have gained gratifying results,but some aspects are still existing defects such as the studies on active ingredients and formula of Scutellaria baicalensis Georgi have not been deeply studied.We propose that human genomics and proteomics researching results should be adopted in the study of Scutellaria Baicalensis Georgi,together with the advanced technologies of bio-chip and biological chromatography being used to elucidate mechanisms at molecular level.

Objective:To explore the effect of two kinds of cardioplegia on cardiomyocyte apoptosis and the related protein expressions in rat hearts. Methods:24 Wistar rats were randomly divided into 3 groups(n=8):Control group,STH-2 group and TTX group. Isolated rat Langendorff and Neely working heart models were estabished. The changes in cardiomyocyte apoptosis were detected by TUNEL.The expression of Bcl-2 and Bax protein were detected by immunohistochemical assay in cardiomyocyte. The expression of Bcl-2 protein in cardiomyocyte was detected by Western blot. Results:Apoptosis index(AI) in STH-2 group(11.20?0.79)% was significantly higher than that in TTX group(6.92?1.10)% and Control group(1.34?0.23)%(P