Objective: To study the effect of volatile oils from Foeniculum Vulgare Mill, anisaldehyde, anethole and water extracts on the percutaneous penetration of 5 fluorouracil. Methods: With Valia Chien horizontal diffusion cell and HPLC method to observe the effect of volatile oils of Foeniculum Vulgare Mill, anisaldehyde, anethole on the percutaneous penetration of 5 fluorouracil. Results: The volatile oils of Foeniculum Vulgare Mill, anethole, anisaldehyde possessed the high promoting action on percutaneous absorption of 5 fluorouracil, and their enhancement ratio are 7.14, 4.17, 9.54, respectively. Conclusion: The volatile oils of Foeniculum Vulgare Mill, anethole, anisaldehyde enhance the skin permeation of 5 fluorouracil effectively.

HL-60 cells treated with 80?g/ml notogensenosidc R_1, were induced to granulocytic differentiation to 68% (among which metamyelocytes 32%, banded neutrophils 30% & segmented neutrophils 6%). Further experiment showed that the ability of the cell to reduce nitroblue tetrazolium (NBT) dye, phagocytosis, as complement receptors and activities of acid phosphatase and ?-glucuronidase were all enhanced after differentiation. There were significant differences between the treated groupand the control group in NBT positive rate (62% v. s. 10%, P

Cytochrome P450(CYP450)is the most important family of enzymes in microsomal mixedfunction oxidase,widely distributed in vivo.CYP450 is involved in the metabolism of many exogenous compounds,the generation of endogenous substances,in particularly,affecting the occurrence and development of the tumors and their drug treatment,in the fields of medical and pharmaceutical research,cytochrome P450 has been very noticeable.

Objective To explore the difference between the results of the cross-disciplinary joint teaching method and the traditional teaching method in medical humanity courses.Method 128 students in one military medical university were selected to be randomly assigned to the study group and the control group,using the cross-disciplinary joint teaching mode (study group,n=64) and traditional teaching mode (control group,n=64) in medical humanity courses.For the joint teaching mode,we compiled the teaching materials of medical humanities,formed the teaching team and organized the academic communication after class by social media.After all courses were finished,the subject cognition and approval of whole students were evaluated by the unified arranged examination and questionnaire.Then the data was processed by SPSS 18.0.Results On the aspect of subject cognition,the total scores of the study group were higher than control group in health law [(76.2 ± 3.34) vs.(72.5 ± 4.76);t=3.32,P=0.000] and medical ethics [(75.3 ±2.93) vs.(68.3 ± 3.10);t=4.01,P=0.000].On the aspect of subject approval,the recognition of the students of the study group in these three subjects were more than those of the control group except the selfevaluation of medical psychology (P=0.000).Conclusion The application of cross-disciplinary joint teaching method on medical humanity courses contributes evidently to the promotion of the medical humanity quality of clinical medical students.To implement this model,however,multi-sided resources are needed for colleges to coor-dinate,for perfect results require more practical exploration.

Objective To study the effects of miR-130b on the neuronal migration in the developing embryonic mice cortex.Methods Pregnancy E15.5 mice were selected and plasmid of miR-130b was injected into the lateral ventricle of the embryonic brain.Two days after eletroporation,murine embryos were collected and cut into frozon coronal slices,then surveyed the neuronal migration under the fluorescence microscope.Results The neuronal migration rates were higher in miR-130b overexpression embryonic cortex,of which 75.1％ eletroporated neurons migrated into MZ,compared with the 22.1％ in control group.On the contrary,only 7.9％ eletroporated neurons stayed in the VZ/ SVZ in the experimental group,compared with the 69.3％ in control group.Conclusions miR-130b accelerates the neuronal migration in the developing embryonic mice cortex,its regulational role is worth studying.

Objective To explore the mechanism ofTiaozhong Huatan Anshen Mixture (THAM). Methods A total of 200 healthy Kunming mice were randomly and equally divided into first, second, third and fourth major groups. Each major group was further equally divided into blank group, control group and high, medium, low dose THAM groups. Each group was given an equal volume of NS, estazolam, or high, medium, or low dose THAM by gavage for 7 d. For the first major group, the changes in number of spontaneous activities after administration were evaluated. For the second major group, 60 min after administration, each group was given pentobarbital sodium (30 mg/kg body weight, minimum dose for all mice sleeping) by intraperitoneal injection, and the sleep time was recorded. For the third major group, 60 min after administration, each group was given pentobarbital sodium (15 mg/kg body weight, maximum dose for 90%-100% of all mice having righting reflex) by intraperitoneal injection. The number of mice falling asleep was recorded, and the sleep rate was calculated. For the fourth major group, mice were sacrificed after continuous administration 15 d. They were quickly removed brain tissue;homogenates were taken out in batches;5-HT and 5-HIAA levels in brain tissues were determined according to fluorescence spectrophotometric.Results Compared with the blank group, all treated groups had a reduced number of spontaneous activities, a shortened sleep latency, a prolonged sleep time, and an increased sleep rate after administration (P<0.01,P<0.05), with the most significant changes seen in the control group and high-dose THAM group (P<0.05), with out statistical significance between the two groups (P>0.05). Compared with the blank group, brain 5-HT and 5-HIAA levels in all treated groups increased in mice (P<0.01,P<0.05), with the most significant changes seen in the high-dose THAM group (P<0.05).Conclusion THAM has a significant sedative-hypnotic effect. The mechanism may be related to increasing brain 5-HT synthesis and metabolism.

OBJECTIVE: To explore the association of XRCC3 gene polymorphisms and haplotypes with laryngeal. METHOD: We selected 4 tag SNPs (rs12432907, rs861536, rs861537, rs861531, rs861531) for the present study. 310 laryngeal patients and 310 healthy control subject were genotyping. The distribution of genotypes and haplotypes in these two group was compared. RESULT: The distributions of rs12432907 was significantly different between these two groups. The CCAG haplotype frequency was higher in laryngeal group than that in control group. But TCAG and TTAG haplotype frequency was were lower in the laryngeal patient than that those in the control subject. CONCLUSION XRCC3 gene polymorphism was associated with the risk of laryngeal patients.
Case-Control Studies ; DNA-Binding Proteins ; genetics ; Genotype ; Haplotypes ; Humans ; Laryngeal Neoplasms ; genetics ; Polymorphism, Single Nucleotide

ObjectiveTo investigate the clinical and imaging features of nonaneurysmal perimesencephalic subarachnoid hemorrhage (NAPSAH).MethodsThe patients with NAPSAH were retrospectively enrolled, and their clinical and imaging features were collected and analyzed.Results In 489 patients with subarachnoid hemorrhage treated over the same period, a total of 23 (4.7%) with NAPSAH were diagnosed with cranial CT and CT angiography.Subarachnoid blood centered in the prepontine cistern in 4 patients (17.39%), interpeduncular cistern in 8 (34.78%), ring pool in 7 (30.43%), and quadrigeminal cistern in 4 (17.39%).Two patients were transferred to other hospital for treatment, and the remaining 21 patients had a good outcome without complications and neurological deficits.Among them, the modified Rankin scale scores in 17 patient were 0, and 4 were 1.Their cardinal symptoms were forgetfulness, irritability, anxiety, and other subjective symptoms.They were improved after receiving psychological counseling and anti-anxiety treatment.ConclusionsThe incidence of NAPSAH is low.The clinical symptoms in patients with NAPSAH are mild, the outcomes are better without obvious complications.

Objective To study the effects of ceftriaxone sodium(Cef) on the seizures and the expression of glutamate transporter (GLT-1) in kainic acid (KA) epilepsy model.Methods Firstly, a chronic spontaneous seizure mouse model was established by unilateral hippocampal injection of KA and monitored by vEEG technique to record seizures.The experimental group received intraperitoneal injection of Cef 200 mg/(kg·d) and the control group received normal saline.Seizure frequency, interictal spike waves and histological phenotypes were recorded to evaluate the function of Cef.Then we use the Western blot to detect the effect of expression for GLT-1.Results Unilateral hippocampal injection of KA 200 ng successfully established the mesial temporal lobe epilepsy model.Cef can reduce the seizures from 2.145 times/day to 1.597 times/day, decreased by 31.2% with a statistical significance(P<0.05).Cef treatment did not significantly enhance the expression of GLT-1.Conclusions Intraperitoneal injection of Cef partially inhibites the seizures of KA model, but the expression of GLT-1 in hippocampus is not enhanced.It is suggested that ceftriaxone may inhibit seizures through other mechanisms.

Frailty is a pre-disabled stage and associated with increased vulnerability to adverse health outcomes. Frailty has high preva-lence in older population. There are 2 principal emerging models of frailty, which are the phenotype model and the frailty index model. Many studies have developed tools to detect frailty, including self-report scales, such as PRISMA-7, Groningen Frailty Indicator (GFI), Ed-monton Frail Scale (EFS) and Simple Frailty Questionnaire (FRAIL);and single assessments, such as gait speed, timed up-and-go test, grip strength, pulmonary function test. Intervention of exercise and/or nutrition are benefical for frail older adults.