Objective of electroacupuncture(EA) of Alzheimer′s disease(AD) rat learning and memory ability and plasma SOD , MDA content in brain P53 ,P21 expression .Methods The normal 36 SD rats ,were randomly divided into sham operation group , model group and EA group .EA group acupuncture “Baihui” ,“Taixi” ,“Zusanli” treatment ,through the Morris water maze test in rats behavioral changes ,spectrophotometric observation of SOD and MDA in rat plasma levels ;observation of rat brain P53 ,P21 ex-pression by immunofluorescence .Results Compared With the model group ,group EA escape latency ,longer in the third quadrant of the activities of the time ,the number of crossing platform position increased ,decreased the elevated plasma SOD level ,MDA lev-el ,reduce the expression of brain P53 ,P21 ,and the difference was statistically significant (P<0 .01) .Conclusion electro-acupunc-ture treatment can improve the Aβ1-42-induced AD rat spatial learning and memory ability ,may be lowered P53 ,P21 protein ex-pression increase SOD activity and MDA .

Objective To investigate the effects of LncRNA MT1JP on the migration and invasion of uveal melanoma cell,and explore its regulatory mechanism.Methods The expression level of LncRNA MT1JP in uveal melanoma cell line and normal retinal pigment epithelium cell line were measured by qRT-PCR.The SP6.5 cell line was divided into three groups,MT1JP group transfect with pcDNA3.1-MT1JP,siMT1JP group transfect with pcDNA3.1-siMT1JP and NC group transfect with negative pcDNA3.1 plasmid.The migration and invasion ability were measured by cell wound scratch assay and transwell assay.Western blot was used to measure the expression level of P53 protein.Results The expression level of LncRNA MT1JP in SP6.5 and M23 cell line was 0.20 ± 0.02 and 0.31 ± 0.01,respectively,which was significantly lower than 1.0 in ARPE-19 cell line (all P ＜0.01).The wound healing rate in siMT1JP group was 61.50 ± 3.70％.while,NC group was 20.0％ ± 2.10％,and MT1JP group was 10.6％ ± 1.7％,there was no statistical difference among three groups (all P ＜ 0.01).The invasion cell number was 75.6 ±6.8 in siMT1JP group,which was significantly more than 29.5 ± 3.9 in NC group,and 10.3 ±2.6 in MT1JP group,there was no statistical difference among three groups (all P ＜ 0.01).The expression level of P53 protein in siMT1JP group was 0.41 ± 0.04,which was significantly lower than 1.0 in NC group (P ＜ 0.01).However,the expression level of P53 protein in MT1JP group was 5.73 ±0.62,which was significantiy higher than 1.0 in NC group (P ＜ 0.01).Conclusion LncRNA MT1JP inhibits the migration and invasion of uveal melanoma cell,which may be associated with upregulation of P53 expression.

Periodical assessment over the actual situation of physician work in a Class One Grade A hospital in Beijing is performed, and some ideas to improve the work are proposed in view of the problems existing in the work.

通过对地震后伤员的特点和易发生的肺部感染并发症等描述,分析呼吸训练对震后伤员预防肺部感染的重要性及必要性,探讨呼吸训练的作用和常用训练方法及其对震后伤员施行呼吸训练的应用意义。

Objective To study the protective effect of glutamine on the intestinal mucosal antioxidation in endotoxemic rats. Methods　Twenty-eight male Wistar rats were randomly divided into three groups, group A:parenteral nutrition supplemented with glutamine, group B:TPN without glutamine,and group C:normal control. Endotoxemia was induced by continous intravenous infusion of lipopolysaccharide(LPS) at a dose of 2 mg/kg per day throughout the 5-day study period. The mucosal protein、DNA、ATP、SOD、MDA、GSH、sIgA were determined. Results The mucosal protein、DNA、ATP、SOD、GSH and sIgA content in endotoxic rats were markedly decreased, MDA was increased as compared with normal control(P＜0.05). The former indices in group A were improved and MDA content was decreased as compared with group B(P＜0.05). Conclusion　Glutamine can improve gut energy metabolism, decrease the extent of mucosal injury of free radicals, and give an protective effect on the mucosal probably by increasing GSH.

MicroRNAs (miRNA) are a class of small non-coding RNA molecules that are rich in content and are highly con-served. miRNA can regulate vital biological processes, including cell division, differentiation, and cell apoptosis. Their expression is dysregulated in many diseases. miRNA are essential in the initiation and development of various cancers. Recent miRNA studies have gradually moved from basic molecular studies of cancer onto areas of clinical application. The use of miRNA as biomarkers for cancer diagnosis and target molecules for cancer treatment is increasingly being identified. This review summarizes the potential applications of miRNA as biomarkers for diagnosis and prognosis of gastric cancer, drug response, and susceptibility prediction.

Acupuncture Points ; Acupuncture Therapy ; Female ; Humans ; Meige Syndrome ; therapy ; Middle Aged

Objective To discuss the clinical features and related factors of psychosis in patients with Parkinson's disease (PD) and assess the prevalence of psychosis.Methods A total of 82 patients with Parkinson's disease and 69 healthy controls were evaluated by Brief Psychiatric Rating Scale and neuropsychiatric questionnaire.For the PD group,the age at PD onset,type and dose of PD medications,and rapid eye movement (REM) sleep behavior disorder (RBD) were recorded.Unified Parkinson's disease rating scale (UPDRS,parts Ⅱ and Ⅲ) and Hoehn-Yahr staging were used for the evaluation of the severity of motor dysfunction,Hamilton depression scale (HAMD) for emotional state,montreal cognitive assessment (MoCA) for cognition,and scales for outcomes in Parkinson's disease-SLEEP (SCOPA-SLEEP) for quality of sleep.Results The prevalence of psychosis was 36.59％ in PD patients and 2.90％ in the control group.The difference between the two groups was statistically significant (P＜ 0.001).Among the 30 PD patients with PD psychosis meeting the NINDS/NIMH criteria,patients experienced illusions,false sense of presence,hallucinations and delusions was 22.0％,14.6％,29.3％,and 7.3％,respectively.Univariate analysis showed that age,levodopa equivalent dosage,SCOPA-SLEEP scores,and prevalence of RBD were significantly higher in thePDP group [(71.1±6.18),(628.7±283.2),(15.3±7.28),60.00％] than in the PDNP group [(66.6±9.25),(461.1±317.2),(9.7±7.64),28.85％] (all P＜0.05).Multivariate analysis showed that Hoehn-Yahr staging was a protective factor (OR =0.039,95％ CI:0.004-0.402),while sleep disorder was a risk factor (OR=1.157,95％CI:1.015-1.320).The PD patients with more psychotic symptoms were much older and with higher SCOPA-SLEEP scores,which had a statistically significant difference (P =0.0042,P =0.0112).Conclusions The prevalence of psychosis is much higher in PD patients than in healthy controls.PD psychosis is correlated with older age,larger levodopa equivalent doses,higher SCOPA-SLEEP scores,and prevalence of REM sleep behavior disorder.

Objective To prospectively study the effects of different fluid resuscitation on kidney function in pediatric patients with sepsis.Methods One hundred and eighteen pediatric patients with sepsis shock who needed fluid resuscitation in Intensive Care Unit,Zhengzhou Children′s Hospital from June 2012 to June 2015 were selected, and they were randomly divided into group A,B and C by random number in the table.The patients received fluid resus-citation of hydroxyethyl starch,50 g/L albumin and 9 g/L saline.After that,neutrophil gelatinase associated lipocalin (NGAL),α-1 microglobulin,urea nitrogen,creatinine,urine volume,the incidence of acute kidney injury (AKI),and the renal replacement therapy rate accepted by the patients were recorded.Then all the data were analyzed with SPSS 19.0 software.Results Serum levels of NGAL and α-1 microglobulin in group A [(103.50 ±1.23)μg/L,(3.32 ± 0.20)mg/L]were higher than those in group B [(93.54 ±1.42 )μg/L,(2.71 ±0.18 )mg/L]and group C [(79.57 ±1.54)μg/L,(2.08 ±0.14)mg/L],which were statistically different(all P <0.05 );serum levels of NGAL and α-1 microglobulin in group B were higher than those in group C,which were statistically different(all P <0.05).Serum levels of urea nitrogen and creatinine in group A[(5.23 ±0.34)mmol/L,(112.00 ±7.12)μmol/L] were higher than those in group B [(3.56 ±0.31)mmol/L,(77.25 ±5.34)μmol/L]and group C[(2.95 ±0.42) mmol /L,(72.12 ±6.34)μmol/L],which were statistically different (all P <0.05);serum levels of urea nitrogen and creatinine in group B were higher than those in group C,which were not statistically different(P >0.05).The urine volume in group A [(0.50 ±0.12)mL/(kg·h)]was less than that in group B[(0.90 ±0.23)mL/(kg·h)]and group C[(1.30 ±0.14)mL/(kg·h)],which was statistically different(all P <0.05);the urine volume in group B was less than that in group C,which was not statistically different(P >0.05).In three groups,there were 13 cases (34.2%)with AKI in group A,9 cases(23.1%)with AKI in group B,and 8 cases(19.5%)with AKI in group C, the incidence of AKI in group C was less than that in group B and group C,which was statistically different(χ2 =12.74,10.43;all P <0.05).All the groups all had children accepted renal replacement therapy,there were 8 cases (21.1%)in group A,7 cases(17.9%)in group B,and 6 cases (14.6%)in group C,and the cases who accepted renal replacement therapy rate in group C were less than those in group A and group B,which was statistically different(χ2 =11.36,8.73;all P <0.05).Conclusions The adverse effect of 9 g/L saline on kidney function is less than hydrox-yethyl starch and 50 g/L albumin,and hydroxyethyl starch has more adverse effects on kidney.Thus,9 g/L saline is a better option for fluid resuscitation in pediatric sepsis patients.

Objective To investigate the clinical result, complication and influential factors of lyophilized and radiation-sterilized morselized allogeneic bone. Methods Between December 1995 and December 2000, 778 cases with implantation of bone allograft were performed. There were 188 females and 590 males with an average age of 37.2 years (range, 6-76 years). The lyophilized morselized allogeneic bone was processed according to American Association of Tissue Bank (AATB) standards and was sterilized by radiation. The bone allograft was rehydrated in fresh blood or in physiologic saline before implantation. The morselized allograft was used to fill defects attributable to various causes such as 84 bone tumors (10.8%), 551 fractures (70.8%), 143 joint fusions (18.4%). After implantation of the bone allograft, drainage was carried out for 2~3 days and antibiotics were used to prevent infection. The average amount of the allograft implanted was 24 g (range, 4-64 g). Results All patients were followed up clinically and radiographically. The minimum length of follow-up was 3 years with an average follow-up period of 59.7 months (range, 36-96 months), 722 allograft unions occurred at a mean of 4.5 months postoperatively (range, 3-8 months). There were 137 complications, included 44 of wound effusion (5.65%), 29 of delayed wound healing (3.72%), 2 of profuse liquid accumulation (0.26%), 43 of fracture non-union (5.52%), 4 of tumor recurrence (0.51%), 9 of fusion failure (1.15%) and 6 of fracture (0.77%). Estimated by the combined Mankin and Komender standards, 688 (88.44%) patients were satisfied with their outcomes while the other 90 (11.56%) patients were dissatisfied. Conclusion The lyophilized and radiation-sterilized morselized allograft is of good tissue compatibility and able to promote bone formation, it can be considered as a convenient, safe, and available material for bone transplantation. The important factors affecting allograft healing are the mechanical stability in the recipient region, local blood supply for the site of grafting and the surgical technology. The major complications of allograft are late infection and re-fracture.