Intraocular lens ( IOL) implantation is the major method to replace the cataract lens. How to improve the biocompatibility of IOL has been the focus of current research. Major reactions after the IOL implantation include endophthalmitis, corneal endothelial edema, iritis, uveitis, and posterior capsule opacification, etc. At cellular level, macrophages, monocytes, fibroblasts and lens epithelial cells can be detected on the surface of IOL. Their adhesion, proliferation migration, and transformation may be induced by the operation related cytokines released into the aqueous humor. Detailed analysis of cytokines profile after IOL implantation may be beneficial to explore the mechanism of posterior capsule opacification.

Objective:To study the relationship of retaining time of tympanic ventilation tube and aural complications. Method:Three-hundred-five patients(659 ears)with otitis media with effusion(OME)received tympanostomy tube insertion. The tube were removed 6-36 months after tube insertion. Then aural complications were recorded in different tube retaining time, followed with a statistic analysis. Result: Fifty-five tubes of 29 patients were removed at 1-6 months after tube insertion, with spontaneous extrusion 3.4%, blocked tube 10. 3%, intrusion into the middle ear O, granulation 'tissue O, cholesteatoma O, otorrhea 6.9%, perforation O. One hundred and ninty tubes of 96 patients were removed at 6-12 months after tube insertion,with spontaneous extrusion 7. 3%,blocked tube 15.6%, intrusion into the middle ear 1%, granulation tissue O, cholesteatoma O, otorrhea 5.2%,perforation O. Three huandred and eight tubes of 156 patients were removed at 12-24 months after tube insertion, with spontaneous extrusion 9%,blocked tube 12.8% ,intrusion into the middle ear 1.3%,granulation tissue 1.9% ,cholesteatoma 0.6%,otorrhea 2.5%,perforation was O. One hundred and sixty one tubes of 83 patients were removed at 24-36 months after tube insertion, with spontaneous extrusion 36.1%, blocked tube 53%, intrusion into the middle ear 6%, granulation tissue 3. 6%, cholesteatoma 2.4%, otorrhea 2.4%, perforation 2.4%. Conclusion:The occurrence of complication didn't increase with time going by when the ventilation tube retained less than two years. However, when the ventilation tube retained more than two years, the occurrence of spontaneous extrusion and blocked tube increased obviously.

Actins ; metabolism ; Animals ; Antigens, CD ; metabolism ; Cadherins ; metabolism ; Carotid Artery Injuries ; metabolism ; pathology ; Endothelium, Vascular ; metabolism ; ultrastructure ; Fluorescent Antibody Technique ; Microscopy, Confocal ; Rats ; Troponin ; metabolism

Cost-Benefit Analysis ; Early Detection of Cancer ; Humans ; Mass Screening ; methods ; Neoplasms ; diagnosis ; epidemiology ; therapy ; Program Evaluation

Objective:To compare the clinical effects of restrictive blood transfusion combined with hyperbaric oxygen preconditioning (HBOPC) and restrictive blood transfusion in the treatment of hip,knee arthroplasty (THA,THA).Methods:40 patients in the period of epidural anesthesia,femoral nerve hysteresis hip and knee arthroplasty were selected and randomly divided into two groups:restrictive transfusion group (maintain 80 g/L≤ Hb ＜100 g/L,n=20) and restrictive blood transfusion combined with HBOPC (HBOPC+maintain 80 g/L =Hb ＜100 g/L,n=20).The red blood cell transfusion,red blood cell transfusion rate,perioperative Hb,blood oxygen saturation (SO2),the incidence of hypotension during operation,hospitalization time and postoperative cerebral infarction,acute pulmonary embolism,pneumonia,myocardial infarction,wound infection rate and 90 days mortality rate were compared between two groups.Results:Compared with the restrictive transfusion group,the postoperative Hb,blood oxygen saturation (SO2) of restrictive blood transfusion combined with HBOPC group were significantly increased(P ＜ 0.05);the red blood cell transfusion,red blood cell transfusion rate,incidence of pneumonia,wound infection rate were significantly decreased (P＜0.05).Conclusion:Restrictive blood transfusion combined with hyperbaric oxygen preconditioning could improve the anoxic state of the hip,knee arthroplasty patients,which could effectively reduce red blood cell transfusion,reduce postoperative complications,has good clinical curative effect.

Objective To investigate the effects of a miRNA family member, let-7e, and a combi-nation of miR-106b and miR-20a on the expression of cytokines by THP-1 cells with Luminex xMAP technol-ogy.Methods The efficiency of transfection was evaluated by immunofluorescence assay after transfecting THP-1 cells with micrONTM mimic negative control (Cy3) for 24 h, 36 h and 48 h.The three miRNA mim-ics (let-7e, miR-106b and miR-20a) were respectively used to transfect the THP-1 cells for 24 h, 36 h and 48 h and the expression of each miRNA was analyzed by qRT-PCR analysis for screening out the optimal transfection time.The transfected THP-1 cells were stimulated with1 mg/L of LPS for 1 h.The Luminex xMAP technology was used to detect the expression of IL-8, interferon-inducible protein-10 (IP-10), mono-cyte chemotactic protein 1 (MCP-1), IL-1α, IL-6, IL-10, TNF-α, IFN-αand IFN-βin the supernatants of cell culture.A statistical analysis was performed to analyze the data obtained by using SPSS16.0 software. Results More than 90% of the transfected THP-1 cells were labeled with red fluorescence.The optimal transfection times for let-7e mimic and miR-106b/miR-20a mimics were 48 h and 24 h, respectively.Com-pared with the corresponding negative control (NC), the expression of IL-8, IP-10 and MCP-1 by THP-1 cells were enhanced after the transfection with let-7e mimic, but were inhibited after the co-transfection with miR-106b and miR-20a mimics.Conclusion The expression of IL-8, IP-10 and MCP-1 were enhanced in let-7e transfected THP-1 cells, but were inhibited in miR-106b and miR-20a co-transfected THP-1 cells.

Objective To evaluate the clinical application of partial splenic embolization in the treatment o hypersplenism caused by schistosomiasis. Methods Twenty four patients with hypersplenism caused by schistosomiasis were treated with peripheral partly splenic embolization with gelf.oam. The counts of white blood cell(WBC) and platelet (PLT) were compared between pre-and post-splenic embolization. Results The extent of splenic embolization were 50% - 75%(mean 59.24%) with post-therapeutic follow-up of 6 months ~ 2 years, showing significant increase of WBC count with preoperative, peak and the latest follow-up counts as (2.15?0.67)?109/L, (12.36?3.24)?109/L and (5.65?1.38)?109/L respectively(t = 11.08, P

Objective To investigate the effect of testosterone on mitotic orientation in rat prostate epithelial cells and the relative differential gene expression.Methods Twenty SPF male SD rats were divided into 2 groups at random and then subjected to castration.One group of rats was administrated with testosterone 3.7 mg daily for 30 days and the control group was only injected with olive oil.Microscopic analysis was performed using immunohistochemistry.Differential gene expression analysis was conducted by gene microarray and RT-PCR techniques.Results In the testosterone-adminis-trated group, there was a significant mitosis orientation parallel to the basement membrane.But in the control group, mito-sis orientation was oriented perpendicular to the basement membrane.Using the gene microarray and RT-PCR techniques, the cell proliferation genes such as Ran, Tgm4 and Wnt2 in Wnt signal pathway were up-regulated in the testosterone group.Conversely, suppressor cell proliferation genes such as Dkk3 and Fas were down-regulated.Conclusions Mitotic orientation of prostate epithelia cells is changed after testosterone administration.Wnt signal pathway and AR singling path-way also have an influence on the mitosis orientation and cell proliferation.

25 is also an important impact factor among various components.