The compositions and contents of fatty acids in the meat and viscera in Chlamys ferreri near Yantai seacoast, Shandong Province were studied in detail. The result showed that the contents of the unsaturated fatty acids in the meat and wastes were 65. 0% and 66. 2%, respectively. Moreover, the contents of the polyunsaturated fatty acids and EPA+DHA were 55. 1% and 37. 5% in the meat, respectively. The contents in the viscera were 48. 8% and 22. 2%, respectively.

Many controversies and unanswered questions surround AIS(adolescent idiopathic scoliosis),the most difficult one is the absence of information about its aetiopathogenesis.Although bracing has been regarded as the standard of care for patients at a high risk of progression,it has never been subjected to rigorous assessment of either its efficacy or effectiveness.With respect to surgical management,although technology has greatly increased the safety with which we can correct spinal deformity and preserve spinal balance,long-term results of these changing methods of management are absent.Surgery can reduce deformity and prevent further progression;thereby decreasing the risk of future cardiopulmonary compromise,but its role in the prevention of other negative long-term effects of scoliosis is not clear.In this paper we discuss present notions about aetiopathogenesis,natural history,non-operative treatment,and surgery.

Objective To observe the effects of repetitive transcranial magnetic stimulation (rTMS) on cognitive function in rats with chronic cerebral hypoperfusion.Methods The animal model of chronic cerebral hypoperfusion was established in 30 Sprague-Dawley rats by surgically induced bilateral common caroid artery stenosis,who were then divided into a control group and a treatment group.The rats in the treatment group were administered with 20 Hz rTMS 4 weeks postoperation for 7days.Another 10 rat subject to sham operation served as blank controls.The cognitive function was assessed by Morris water maze (MWM) at the time points of 2nd,3rd,4th and 5th days after ending of the 4 weeks of tretment.The morphologic changes of hippocampus neurons were observed with HE staining.The apoptosis was examined by TUNEL,the expression of Bcl-2 or Bax protein was determined using immnunohistochemistry assay.Results At all the time points the MWM escape latency in the rTMS group was shorter than that in the control group (P ＜ 0.01).The percentage of crossing the corresponding platform during the same time period in platform quadrant in the rTMS group was significant higher than that in the control group (P ＜ 0.01).Compared with the control group,the treatment group demonstrated significantly decreased percentage of neuronal apoptosis (P ＜0.05),as well as increased expression of Bcl-2 protein (P ＜0.01) and reduced expression of Bax protein (P ＜ 0.01).Conclusion rTMS can improve the cognitive dysfunction in rats caused by chronic cerebral hypoperfusion,probably through inhibiting neuronal apoptosis in hippocampus region.

Crowns ; Dentures ; Humans

Objective To study the effect of fetal transplants on the fu nctional recovery after spinal cord transection in new born and adult rats. Meth ods The spinal cord tissue at E14 of a rat fetal was transferred into the hemise ction cavity at lumbar spinal cord in the newborn and adult rats. 4, 8, 12 weeks after the operation, spinal cord tissue morphology, combined behavioral score ( CBS), somatosensory evoked potentials (SEP), and motor evoked potentials (MEP) w ere examined. Results The transplanted tissue survived in the host spinal cord. There was a statistical difference in CBS between the newborn graft group and th e adult graft group. The peak latencies of early waves in SEPs and MEPs of the n ewborn graft group were superior to those of the adult graft group (P

Group D (P

A new way is proposed to design control panels of precise titration in infusion.Based on the basic principles of hydrodynamics,this method implements the control of medicine flow by adjusting the tube size.With high precision and low cost,it is beneficial to practical requirements in chinical application.

OBJECTIVE: To observe monocyte chemoattractant protein 1 (MCP-1) changes in ischemic tissue during the process of angiogenesis induction in ischemic limbs by autologous mesenchymal stem cell (MSC) transplantation.METHODS: Twenty male Sprague-Dawley rats were randomized to 2 groups (n = 10): model and MSC transplantation. Femoral and tibial bone marrow was taken to isolate and culture MSCs by percoll density gradient method. Cells of the 3~(rd) or 4~(th) passage were used for transplantation. Severe bilateral hind limb ischemia was surgically created in each group rats. Two hours after model establishment, MSCs (1×10~(11)/L) were infused into the ischemic region of rats from the MSC transplantation group, and the model group received the same amount of phosphate buffered saline. Collateral artery formation was determined by angiographic analysis and histological assessment. CD68~+ macrophage infiltration was examined by immunohistochemistry. MCP-1 protein expression in the plasma and ischemic tissue was detected by enzyme-linked immunosorbent assay. MCP-1 mRNA expression in ischemic tissue was detected by reverse transcription-polymerase chain reaction.RESULTS: At postoperative 28 days, treatment with MSC transplantation lead to collateral vessel formation, and immunohistochemistry demonstrated that CD68~+ macrophage infiltration was lower compared with the model group. MCP-1 protein and mRNA expression in the plasma and ischemia tissue was significantly lower in the MSC transplantation group than in the model group (P < 0.05).CONCLUSION: Following MSC transplantation, MCP-1 may play an important role in ischemia-induced angiogenesis. This indicates that MCP-1 would become one possible target molecule for modulating inflammatory angiogenesis by MSC Transplantation.

Flavones and flavonols belong to flavonoids that have anti-cancer activities. In order to explore molecular mechanism and inhibitory effects of flavones and flavonols on human esophageal carcinoma cells, the inhibition of proliferation and the induction of G2/M cell cycle arrest in KYSE-510 cells and OE33 cells treated with three flavones (luteolin, apigenin, chrysin) and three flavonols (quercetin, kaempferol, myricetin) were analyzed by MTT array and flow cytometry. Among these compounds, luteolin and quercetin were the most active flavonoid to inhibit the proliferation of KYSE-510 cells and OE33 cells, respectively. The genes related to cell cycle control were analyzed by gene chip, after KYSE-510 cells and OE33 cells were treated by luteolin and quercetin, respectively. The results were shown that the expression of p21waf1 was induced and the expression of cyclin B1 was suppressed in KYSE-510 cells, and that the expression of GADD45? and 14-3-3? were induced and the expression of cyclin B1 was suppressed in OE33 cells. These results were verified by real-time RT-PCR and Western-blot. The comparative effects of all six compounds on the regulation of these gene expressions at the mRNA and protein levels were also analyzed by real-time RT-PCR and Western-blot. The results were shown that p21waf1, GADD45?, 14-3-3? and cyclin B1 were the target genes which mediated the effects of flavones and flavonols on induction of cell cycle arrest in KYSE-510 cells and OE33 cells.

Two human esophageal cancer cell lines (KYSE-510 and OE33) were chosen as the tumor model to explore molecular mechanism of flavones and flavonols on induction of apoptosis. Effects of flavones (luteolin, apigenin, chrysin) and flavonols (quercetin, kaempferol, myricetin) on induction of apoptosis in KYSE-510 cells and OE33 cells were observed by DNA fragmentation, acridine orange staining and flow cytometry analysis. The results of real-time RT-PCR and Western-blot analysis showed that the treatment of KYSE-510 cells and OE33 cells with flavones and flavonols induced the expression of PIG3 at the mRNA and the protein levels. Western-blot analytical results further showed that induction of PIG3 caused apoptosis in both esophageal cancer cells through the mitochondrial pathway in a p53-independent manner, and p63 and p73 may be responsible for the induction of PIG3.