Axillary reverse mapping (ARM) is anatomic theoretical foundation based on breast and upper limb lymph axillary lymph injected into different lymphatic populations, ARM can display upper limb lymphatic channels through different tracers during the periods of axillary lymph node dissection and sentinel lymph node dissection, to reduce the rate of postoperative upper extremity edem.Although ARM has a positive effect, it exists a controversy in safety and the relationship between its node and sentinel lymph node.

Pim-3,a new member of the the proto-oncogene pim family which expresses serine and threonine kinase activity,shows a high similar to pim-1 and pim-2 at the function,structure and sequence.Pim-3 plays an important regulative role in the cell cycle transform,cell apoptosis and occurrence of the malignant tumors by phosphorylating numerous specific substrates.

The separation of digestive system cancer stem cells (CSCs) is the first step of the study of stem cell.At present,there are three methods of separation of the digestive system CSCs:cell phenotype sorting,side population cell sorting and serum-free culture.The separation of digestive system CSCs is based on these three methods or be improved to a higher proportion of CSCs.

Based on the theory of cancer stem cells (CSCs),people have been searching for the treatments of malignant cancers.Gastric cancer side population cells (SP) have the characteristics of CSCs.Searching for the molecular targeted therapy strategy of gastric cancer which embarks from the gastric cancer SP and is based on the theory of CSCs provides a new direction for the treatment,early diagnosis,therapeutic effect and prognosis of gastric cancer.

Objective: To find the reason of risk on medical equipment. Methods: Based on RCA, to adopt the event classification to evaluate the X-ray equipment, high-frequency electro tome, electro-cardiac monitor, infusion pump, ventilator, defibrillators and infant incubator, discuss the root cause of medical risk. Results:To deduce the medical equipment risk by PM and quality control. Conclusion:With the analysis of quality control, the application of RCA is gradually explained in management of medical equipment.

Portal vein tumor thrombus is a common complication of primary liver cancer,in the event,and often indicates that his condition has office in the late irreversible,the prognosis is extremely poor.This article describes the treatment of portal vein tumor thrombus of existing methods,to promote muhidisciplinary treatment,improve survival in patients with advanced liver cancer.

Objective: To elucidate the relationship between Notch3 expression and chemosensitivity of human colon carcinoma cell line SW620 to topotecan. Methods: Notch3 siRNA was transfected into SW620 cells, and the expression of Notch3 in SW620 cells was examined by Western blotting. After transfected with Notch3 siRNA for different time peri-ods, SW620 cells were further treated with topotecan, and the proliferation of SW620 cells was detected by MTT assay; the apoptosis of SW620 cells was detected by Hoechst 33342 staining and flow cytometry. Caspase-3 activation in SW620 cells was examined by caspase-3 activation kit. Results: Notch3 siRNA transfection remarkably inhibited Notch3 protein expression in SW620 cells. The IC_(50) of topotecan in Notch3 siRNA-transfected group was significantly decreased compared with that in the Ctrl siRNA group (P <0.05). Silence of Notch3 expression in SW620 cells by Notch3 siRNA remarkably promoted apoptosis (P < 0.05) and caspase-3 activation (P < 0.05) of SW620 cells induced by topotecan. Conclusion: Notch3 down-regulation by siRNA in SW620 cells can enhance the chemosensitivity cells to topotecan.

Primary liver cancer is a high incidence and common malignant tumor in China. Portal vein tumor thrombus is one of the biologic marks of advanced liver cancer and difficult to be cured, and the prognosis is extremely poor. This paper systematically describes the formation mechanism, diagnosis,classification and prognosis of portal vein tumor thrombus. It would provide a theoretical basis for this clinical problem in order to reasonably understand and actively treat it.

Type-2 innate lymphoid cells ( ILC2 ) is a new member of the innate lymphoid cell family which has been recently discovered. These cell arise from lymphoid progenitors in the bone marrow and,under the control of the transcriptional regulators and Gata3,producing IL-5,IL-9 and IL-13 is a sign of the matura-tion. These cells are critical components of the innate immune response to parasitic worm infections and have al-so been implicated in the pathogenesis of asthma. This paper summarizes the role of ILC2 in the pathogenesis of asthma and its therapy research progress.

Objective:To construct pEGFP-C1-Smad4 expression vector and to observe the influence of Smad4 over-expression on the proliferation of human gastric cancer SGC7901 cells and its relationship with nuclear factor kappa B (NF-?B). Methods:Recombinant expression vector pEGFP-C1-Smad4 was constructed and was used to transfect human gastric cancer SGC7901 cells. EGFP expression in transfected cells was detected by fluoroscopy. Smad4 and NF-?B expression in transfectant was examined by Western blotting. Effect of Smad4 over-expression on activation of NF-?B and proliferation of transfected SGC7901 cells were examined by electrophoretic mobility shift assay (EMSA) and MTT assay,respectively. Results:Expression of EGFP in transfected SGC7901 cells was observed under fluorescence microscope. Smad4 was over-expressed in transfected SGC7901 cells,accompanied by down-regulation of NF-?B p65 expression in the tranfectants. EMSA and MTT demonstrated that Smad4 over-expression significantly inhibited the activation of NF-?B and the proliferation of SGC7901 cells (P