1.Determination of serum soluble interleukin-6 receptor and soluble gp130 levels in patient with pregnancy induced hypertension and its significance
Chinese Journal of Obstetrics and Gynecology 2001;36(1):18-19
Objective To observe the changes of serum soluble interleukin-6 receptor (sIL-6R) and soluble gp130 (sgp130) in patients with pregnancy induced hypertension. Methods Enzyme linked immunosorbent assay (ELISA) was used to determine serum sIL-6R and sgp130 levels in 40 patients with pregnancy induced hypertension (study group), 20 normal non-pregnant women (control group I) and 20 normal pregnant women (control group II). Results In study group, sIL-6R was (196.7±12.9) μg/L and sgp130 was (379.4±79.3) μg/L. In control group I, sIL-6R was (174.8±46.2) μg/L and sgp130 was (273.6±28.3) μg/L. In control group II, sIL-6R was (174.4±48.3) μg/L and sgp130 was (254.4±34.7) μg/L. SIL-6R and sgp 130 were higher in study group than those in control groups with significant difference (P<0.01). In study group, the more severe the patients, the higher the sIL-6R and sgp130 levels. There was significant difference (P<0.01). There was no difference in sIL-6R and sgp130 levels between control groups (P>0.05). Conclusions Serum sIL-6R and sgp130 levels are related to the development of pregnancy induced hypertension.
2.Effect of ionizing radiation combined with inhibitors or inducer of autophagy and apoptosis on MCF7 cell
Yali QI ; Jun WANG ; Yan LI ; Hongyan WANG ; Shouliang GONG
Chinese Journal of Radiological Medicine and Protection 2010;30(3):263-266
Objective To detect the inhibiting effects of ionizing radiation combined with inhibitors or inducer of autophagy and apoptosis on MCF7 cell line,and to provide the evidence for human breast cancer therapy radiation.Methods MCF7 cells were exposed to X-rays and randomly divided into 4 groups,including 0 Gy,4 Gy,4 Gy+rapamycin,4 Gy+3-MA,and 4 Gy+z-VAD-fmk groups,including 0 Gy,4 Gy,4 Gy+rapamycin,4 Gy+3-MA,and 4 Gy+z-VAD-fmk groups,respectively.The growth doubling time was calculated by MTT method.The specific protein expressions of LC3 autophagy and beclinl were detected by using Western blot and the difference of Drotein contents wasLC3 autophagy and beclinl were detected by using Western blot and the difference of Drotein contents was compared.The percentage of apoptosis of MCF7 cells was measured by flow cytometry (FCM).Resuits The growth doubling time of MCF7 cells in 4 Gy group wag longer than that in O Gy group(t=4.41,P
3.MicroRNA target predicition based on SVM and the optimized feature set.
Baowen WANG ; Xiaoyang QI ; Changwu WANG ; Wenyuan LIU ; Yali SI
Journal of Biomedical Engineering 2013;30(6):1213-1218
MicroRNA (miRNA) is a family of endogenous single-stranded RNA about 22 nucleotides in length. Through targeting 3' UTR of message RNA (mRNA), they play important roles in post-transcriptional regulatory functions. For further research of miRNA function, the identification of more miRNA positive targets is needed urgently. Aiming at the high-dimensional small sample data sets in miRNA target prediction, an algorithm of eliminating redundant features is proposed based on v-SVM in this paper, and classification and features selection are also fused. The algorithm of eliminating redundant features optimizes the combination of features, and then constructs the best features combination which can represent miRNA and targets interaction model. The prior parameter v (0 < u < or = 1) controls the compression proportion of data set and selects more distinguishing support vectors. Finally, the classifier model of miRNA target prediction is built. The unbiased assessment of the classifier is achieved with a completely independent test dataset. Experiment results indicated that in both classification recognition and generalization performance of miRNA targets predicition, this model was superior to the present machine learning algorithms such as miTarget, NBmiRTar and TargetMiner, etc.
MicroRNAs
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Models, Theoretical
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Support Vector Machine
4.MRI Workflow Optimized by Tim Technology
Haitao ZHAO ; Shun QI ; Jun LU ; Yali GE
Chinese Medical Equipment Journal 2003;0(10):-
The technology of Tim can support multiple seperated matrix coil units while the units can be freely assorted and seamlessly joined to form the a total imaging matrix possessing huge field of view.This paper analyzes the role of Tim which can help to improve extreme flexibility of examination,to possess more signal-to-noise(SNR),to achieve superior " local" resolution,and to possess faster scan time.It is concluded that Tim technology have the strong advantages at improving and optimizing MRI workflow.
5.Application of dexmedetomidine in functional endoscopic sinus surgery during the recovery period of general anesthesia
Qi WANG ; Dengfeng DING ; Yali LI ; Yajing GAO
Modern Clinical Nursing 2015;(6):44-46
Objective To study the effect of dexmedetomidine in the functional endoscopic sinus surgery (FESS) on the recovery period of general anesthesia. Methods Fifteen min before the end of surgery, 40 FESS patients were treated with intravenous infusion of dexmedetomidine at 0.6μg/kg. The occurrence of cough response, degree of pain and agitation in patients were observed. Result The response score of choking cough of the patients with intravenous infusion of dexmedetomiindine was (1.2 ± 0.5), the score of VSA was (1.9 ± 0.5), and the degree of agitation was (1.2 ± 0.4). Conclusion For those undergoing FESS, postoperative use of dexmedetomidine 15 min before the end of surgery, can not only have an effective effect for reducing the incidence of choking cough and agitation and but also decrease the pain degree so that the patients can live through the general anesthesia recovery period.
6.Effects of ionizing radiation on proliferation and invasion of MCF-7 cells transfected with pcDNA3.1-Egr-1-AIF△1-480 plasmid
Yali QI ; Hongbin LIU ; Zhongwei XIE ; Yanjun LIU ; Jianfeng WANG
Journal of Jilin University(Medicine Edition) 2014;(5):929-932
Objective To investigate the influence of truncated apoptosis inducing factor (AIFΔ1-480 ) on the proliferation and invasion of MCF-7 cells,and to clarify the possibility of promoting cancer gene-radiotherapy. Methods The human breast cancer MCF-7 cells were transfected with AIFΔ1-480 recombinant expression vector pcDNA3.1-Egr-1-AIFΔ1-480 (pE-AIFΔ1-480 )mediated by Egr-1;24 h after 2 Gy X-ray irradiation,MTT assay and Transwell invasion assay were performed to measure the changes of cell proliferation and invasion.The MCF-7 cells were diveded into normal control,pcDNA3.1,pE-AIFΔ1-480 ,2 Gy irradiation and pE-AIFΔ1-480+ 2 Gy irradiation groups.Results After transfection and 2 Gy X-ray irradiation,the cells proliferated very fast in normal control, pcDNA3.1 and pE-AIFΔ1-480 groups, and the proliferation regularity was similar. Compared with normal control group,the cell proliferation abilities were significantly decreased in 2 Gy irradiation and pE-AIFΔ1-480 + 2 Gy irradiation groups (P<0.05 ), and it was more obvious in pE-AIFΔ1-480 + 2 Gy irradiation group, and it was significant lower than that in 2 Gy irradiation group (P<0.05).The number of the cells permeating membrane was basically same in normal control,pcDNA3.1 and pE-AIFΔ1-480 groups;compared with normal control group,they were significantly decreased in 2 Gy irradiation and pE-AIFΔ1-480+ 2 Gy irradiation groups(P<0.05 or P<0.01);and it was more significant in pE-AIFΔ1-480+ 2 Gy irradiation group than that in 2 Gy irradiation group (P<0.01). Conclusion AIFΔ1-480 and ionizing radiation could inhibit the proliferation and invasion of human breast cancer MCF-7 cells,both of them have a synergistic effect,and Egr-1 promoter can enhance the suppression effect under radiation conditions.
7. Effects of Smac overexpression mediated by triple-targeting on apoptosis and cell cycle progression of breast cancer MDA-MB-231 cells
Journal of Jilin University(Medicine Edition) 2018;44(1):90-94
Objective: To obtain the conditionally replicative adenovirus with triple-targeting Smac overexpression using gene recombination technology, and to explore its effects on the apoptosis and cell cycle progression of MDA-MB-231 cells. Methods: The triple-targeting Smac overexpression vector pShuttle-Egrl-Smac-HRE-hTERT-ElA-ElBp-ElB55K was constructed by gene recombination technology, which was recombined with the skeleton vector pAdEasy in the BJ5183 bacteria (AdEasy-l +) to obtain the conditionally replicative adenovirus CRAd. pE-Smac. After the MDA-MB-231 cells were infected with CRAd. pE-Smac, the cancer cells were mimiced into hypoxic status with chemical reagent CoCl2, then control group, CRAd. pE-Smac group, hypoxia group and CRAd. pE-Smac + hypoxia group were set up; the cells were irradiated with 4 Gy X-rays, and each group was divided into nonirradiation group and irradiation group. The Smac protein expression was detected by Western blotting assay, the apoptotic rates and the percentages of cells at different phases were detected by flow cytometry. Results: The Western blotting results showed that the Smac protein expressions were increased after infection of CRAd. pE-Smac, hypoxia and 4 Gy irradiation, especially in CRAd. pE-Smac + hypoxia+4 Gy irradiation group. The FCM results showed that the apoptotic rates in CARd. pE-Smac, hypoxia, CARd. pE-Smac + hypoxia group were increased compared with control group (P<0. 05 or P<0. 01), and the apoptotic rates of cells irradiated with 4 Gy were significantly increased compared with the unirradiated cells (P<0. 05 or P<0. 01), especially in CRAd. pE-Smac + hypoxia + 4 Gy irradiation group; the percentages of the cells at S and G2/M phases in irradiation groups were significantly increased (P<0. 05 or P<0. 01), which had the similar regularity with the apoptotic change. Conclusion: After the MDA-MB-231 cells are infected with the conditionally replicative adenovirus CRAd. pE-Smac and treated with hypoxia and irradiation, the triple-targeting Smac overexpression can be achieved, and it has the role of promoting the cancer cell apoptosis and inducing the G2/M arrest.
8.Effects of Active Fraction of Angelica Sinensis Radix on Immunological Function in Mice under High Altitude Hypoxia Condition
Fangyu AN ; Yongqi LIU ; Yali LUO ; Yingdong LI ; Xuesong LIU ; Qi ZHANG ; Lulu CAI ; Lijiao SUN
Chinese Journal of Information on Traditional Chinese Medicine 2015;(2):51-54
Objective To explore the intervention effect of active fraction of Angelica Sinensis Radix in mice under high altitude hypoxia condition. Methods Totally 72 healthy SPF mice were randomly divided into control group (K), model group (M), Rhodiola rosea group, and active fraction of Angelica Sinensis Radix groups (B, C, X). The mice were administerted corresponding treatment by gavage for 21 days. Control mice were given normal saline in same volume. From the 8th day, all mice excepted control mice were exposed to high altitude hypoxia cabin after 0.5 hour gavage treament. On the 22nd day, after got out of the cabin and their body weight were measured, mice were put to death through eyeball blood sampling to prepare splenic lymphocyte suspension. The proliferation and transformation capacities of lymphocyte cell and killing activity of NK cells were detected by MTT. The content of IL-2 in the serum of mice in each group were detected by ELISA. Results Compared with the control group, the body weight of mice, the proliferation and transformation capacities of lymphocyte cell, the killing activity of NK cells, and the content of IL-2 were all significantly decreased (P<0.05, P<0.01). Experiment tests showed that the proliferation and transformation abilities of lymphocyte cell and the killing activity of NK cells were all increased in the mice of group B, C, and X compared with those of the model group (P<0.05, P<0.01). The stimulate index of lymphocyte cell was raised after X intervention compared with those of the model group (P<0.05). The content of IL-2 in the serum was enhanced after intervention of active fraction C and X of Angelica Sinensis Radix compared with those of the model group (P<0.05, P<0.01). Conclusion Active fraction of Angelica Sinensis Radix shows increasing immunological function of mice exposed to hypoxia.
9.The differential expression of apoptosis-associated genes in human gastric cancer MGC803 cells induced by diallyl trisulfide
Yali TAN ; Hao JIANG ; Wenxiang DAI ; Xiaoping WU ; Zhangwen TANG ; Qi SU
Chinese Pharmacological Bulletin 1986;0(06):-
Aim To investigate the differential expression of apoptosis-associated genes in human gastric cancer MGC803 cells induced by diallyl trisulfide(DATS).Methods Growth inhibition against MGC803 cells was assayed by MTT assay;The apoptosis induced by DATS was assessed by Flow cytometry and fluorescent microscope.The apoptosis-associated gene expression of MGC803 cell treated with DATS was determided by Human Apoptosis Gene Array.Apaf-1 and SODD genes were confirmed by RT-PCR.Results DATS had significant growth inhibitory activity against MGC803 cells,inhibition ratio increased from 11% to 78% at 4,8,12,16 and 24 mg?L-1 for 72 h(P
10.Effect of ionizing radiation on apoptosis of lung cancer H460 cells and its mechansim
Jing ZHANG ; Zhicheng WANG ; Dali ZHAO ; Xiaoqian LU ; Zhiyuan SHEN ; Yali QI
Journal of Jilin University(Medicine Edition) 2017;43(3):522-526
Objective:To explore the effect of ionizing radiation on apoptosis of lung cancer H460 cells after ATRX was silenced by RNAi and its mechanism.Methods:The lentivirus expression vectors targeting ATRX were transfected into the 293T cells,and the lung cancer H460 cells were infected with lentivirus twice,and the ATRX silenced cell model was obtained after puromycin positive screening,then they were named as sh-ATRX1-H460,sh-ATRX2-H460,and sh-ATRX3-H460 cells;the sh-control-H460 cells were regarded as control cells.The cells were divided into sh-control-H460 group and sh-ATRX3-H460 group,accroding to the silencing results and were irradiated by 0,2 and 8 Gy X-rays.The expression levels of ATRX,poly(ADP-ribose) polymerase 1(PARP1),and caspase-3 proteins were measured by Western blotting method;the apoptotic rate was measured by flow cytometry and AnnexinⅤ-FITC/PI kits.Results:The lung cancer cell model of sh-ATRX3-H460 silenced by ATRX was obtained successfully.After 2 and 8 Gy X-ray irradiation,compared with before irradiation,the expression level of ATRX protein in sh-control-H460 group was increased,while there was no expression of ATRX protein in sh-control-H460 group;compared with before irradiation,the apoptotic rates of cells in two groups were increased(P<0.05 or P<0.01);the apoptotic rate in sh-ATRX3-H460 group was significantly higher than that in sh-control-H460 group after 8 Gy X-ray irradiation (P<0.01).The expressions of cleaved PARP1 in the cells in both two groups after 2 Gy and 8 Gy X-ray irradiation were increased and showed similar rule.The expression level of procaspase-3 protein in sh-control-H460 group had little change,and it was increased significantly in sh-ATRX3-H460 group after 8 Gy X-ray irradiation.Conclusion:ATRX silencing can be achived by RNAi,then the silencing could increase the apoptosis induced by irradiation and its mechanism may be related to the PARP1-caspase-3 pathway.