1.Study on HPLC-DAD-ELSD Fingerprint in Different Species of Dendrobii Caulis
Yuanhui SHI ; Li GUO ; Li XU ; Puyan HAO ; Jiajiang CHEN ; Lian WEI ; Fangli LUO ; Tingmo ZHANG ; Yu HAN
World Science and Technology-Modernization of Traditional Chinese Medicine 2013;(6):1329-1332
This study was aimed to set up the fingerprint chromatograms of chemical principles of Dendrobii Caulis by HPLC-DAD-ELSD. This analysis was performed at 30°C on a Dikma Cl8 column (250 mm × 4.6 mm, 5 μm) with a mobile phase consisting of acetonitrile-0.5% glacial acetic acid, gradient elution, at the flow rate of 1.0 mL?min-1 with DAD and ELSD detection. The HPLC fingerprint of Dendrobii Caulis was established. And 9 fingerprint peaks were marked. The similarity among different species was low. This method was stable, reliable and repeatable. It is useful for the study on the relationship between chemical components and species of Dendrobii Cauli.
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