Objective To observe the effect of Pirarubicin (THP) with other chemical agents in treating hematopoietic Malignancies. Methods 90 inpatients diagnosed as hematopoietic malignancies between January 2000 to December 2005 were treated with THP with other chemical agents. 30 had not been treated previously ,(untreated) while 60 were relapsing or resistant to previous treatment. Acute leukemia(AL) was 60 cases, Malignancy lymphoma(ML) was 21 cases, Multiple myeloma(MM) was 9 cases. Results The complete response(CR) rate among AL untreated and relapsing/resistant groups were 91.7% and 38.5%; 71.5% of response rate were malignancy Lymphoma(ML); 67.8% of response rate ware multiple myeloma(MM).The total response rate was 80.0%. The main side-effect of THP is bone marrow depression which led to fever and bleeding with rates 30.2%, 20.5%. The non-bone marrow side effects were tolerable. Damage to the liver was a prominent occurrence 20.8%. Conclusions THP was effective as the first line anti-tumor drug with other chemical agents in treating hematopoietic malignancies, its side-effects were mild and well tolerable.

Objective To estimate the effect of sodium arsenite(AS2O3)in treating adult chronic myeloid leukemia(CML).Methods 30 inpatients diagnosed as CML from December 2002 to January 2008 were treated with AS2O3,of which 16 cases had not been treated previously,while 14 cases were relapsing or resistant to previous treatment.Results The complete response rate was 63.3%,while the partial response rate was 20.0%,non-response rate was 16.7%.The total response rate was 83.3%.Conclusion AS2O3 is an effective drug in treating CML with side effects being mild and well tolerance.

Objective To investigate myostatin gene mRNA expression in the muscle tissue from patients with muscle weakness suffering from different illness.Methods The clinical data of our patients were all from the Muscular Disease Center,Department of Neurology,People' s Liberation Army General Hospital.A total of 75 patients suffered from muscular weakness were included consecutively.Skeletal muscle biopsies were obtained with informed consent from all 75 patients.The diagnosis was confirmed by two senior doctors for muscular disease according to the clinical feature,the results of electromyography,serum creatine kinase activity and histopathological examination.Among of them,21 cases were diagnosed as polymyositis,15 cases progressive muscular dystrophy,5 cases neurogenic amyotrophy,4 cases chronic muscle fiber damage,4 cases mitochondrial myopathy,4 cases lipid storage myopathy,4 cases myotonic dystrophy,3 cases muscular dystrophy in adults,2 cases dermatomyositis,and 2 cases inclusion body myositis.There were 2 cases characterized by pure high activity of creatine kinase.And the other 9 cases were diagnosed as non-neuromuscular disease.The expression of myostatin gene mRNA in muscle tissue was evaluated by reverse transcription polymerase chain reaction method,with glyceraldehyde-3-phosphate dehydrogenase as internal reference.Results The expression of myostatin gene mRNA was detected in 63 patients,but not in other 12 cases,and the percentage of positive expression was 84％.The expression index was with great variation,from 0 to 3.52.In positive cohort,the index was correlated positively with the duration of disease (r =0.236,P =0.041).The activities of creatine kinase in positive expression cohort were higher than that of negative one,but nonsignificantly.Conclusion The expression of myostatin gene mRNA in muscle tissue may not correlate to the entity of atrophic muscular disease because of its great variation.

Aim To study the neuroprotective effect of Acteoside against MPTP-induced mouse model of Parkinsons disease (PD) and its mechanism. Methods The behavioral testing of C57 mice was assessed using spontaneous movement and rotarod test. DA levels in striatum were measured using HPLC-EC. Dopaminergic neurons in the substantia nigra were observed by immunohistochemical staining with an anti-Tyroxine hydroxylase (TH) antibody. Moreover, the mechanism of the neuroprotective effect of acteoside was investigated using Western blot analysis with an anti-?-synuclein antibody in the substantia nigra and striatum. Results ① Compared with control, MPTP lesion significantly reduced the number of spontaneous movement and latent period of mice on the rotating rod (both P

Recently,the techniques for proteomics have made remarkable progress.They are widely used in various fields of life sciences,providing strong technical supports for relevant researches.Especially,proteomics technology has made prominent contributions to drug development and mechanism studies,magnificently improving the efficiency of discovering new drugs.This paper summarizes the classical method and new technology of proteomics.Also,its applications in drug study,including targets screening,mechanism studies,drug toxicology,researches of resistance mechanisms and clinical medical studies have been reviewed.

Objective To investigate the expression levels and the roles of induced nitric oxide synthase(iNOS) in rats′ kidney after interruption of blood flow and the following ischemia-reperfusion(IR)injury.Methods Rats were divided into 3 groups:interruptions(5,15,30 min),IR(2,5,8,24,48,72 h),control groups.They were killed at different time points.Kidney tissue samples were studied by immunohistochemical method.Results iNOS increased significantly(P

The regularity and the preventive and curative measures about the post-operative infections in 3 orthotopic cardiac transplant recipients were reviewed.The results showed that the bacterial infections and the opportunistic fungal infections were common within 1～2 months after the cardiac transplantation.The sensitive antibiotics could completely control the infections.More than 3 months after surgery,the infections were gradually reduced.It was suggested that the prevention and treatment of the post-operative infections should be reinforced in the early stage of transplantation.

Aged ; Catheter Ablation ; adverse effects ; Echocardiography ; Heart Atria ; Humans ; Male ; Postoperative Complications ; diagnostic imaging ; Tachycardia, Supraventricular ; diagnostic imaging ; surgery ; Thrombosis ; etiology

Objective To understand head nurse's leadership behavior and analyze the influence of it on nurse's involvement and self-efficacy.Methods General information questionnaire was taken,and the data of 280 nurses to head nurse's leadership behavior,involvement and self-efficacy were investigated.Results There were differences in nurse's involvement and self-efficacy between head nurse's transformation and transaction leadership behavior (F=4.39,2.37,2.94,2.17,P ＜0.05).The transformation leadership behavior dimensions were positive correlation with nurse's involvement and self-efficacy,P＜0.05.Conclusion Head nurse should fully play a positive role of transformation leadership behavior,in order to promote involvement and improve self-efficacy.

Aim To study the effect of echinacoside on behavior and proteins expression from substantia nigra and striatal tissue in MPTP mouse model of Parkinsons disease(PD)and discover the mechanism of its potential dopaminergic neuroprotective effect in the protein level.Methods The mouse model of PD was induced by 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)and the behavioral analysis of C57BL/6 mice was performed by using spontaneous movement and rotarod test.A proteomic approach based on 2-dimensional electrophoresis(2-DE),mass spectrometry(MS)and figure analysis was used to evaluate the effect of echinacoside on the behavior and the protein expression in substantia nigra and striatal tissue in C57BL/6 mice after MPTP administration.Results ① Compared with control,MPTP lesion significantly reduced the number of spontaneous movement and latent period of mice on the rotating rod(both P