1.Detection of HPV in cervical scrape specimens of cervical neoplasia using the polymerase chain reaction.
Seung Chul KIM ; Hak soon KIM ; Ju Cheol SONG ; Seo Ok KANG ; Young Bum CHA ; In Kwon HAN ; In Geol MOON ; Won Hee HAN ; Chong Taek PARK
Korean Journal of Obstetrics and Gynecology 1992;35(9):1269-1279
No abstract available.
Polymerase Chain Reaction*
2.Detection of hepatitis viral nucleic acid sequences using polymerase chain reaction.
Korean Journal of Infectious Diseases 1991;23(4):229-233
No abstract available.
Hepatitis*
;
Polymerase Chain Reaction*
3.The Clinical Applicability of PCR and FISH in the Detection of Y-chromosome from Fetal Nucleated Red Blood Cells in Maternal Blood.
Jae Hyun CHUNG ; Kwan Ja JI ; Soon Ha YANG ; Jung Mi OH ; Cheong Rae ROH ; Young Kyu MOON ; Syng Wook KIM ; Je Ho LEE
Korean Journal of Obstetrics and Gynecology 1997;40(12):2692-2697
No abstract available.
Erythrocytes*
;
Polymerase Chain Reaction*
4.Detection of male-specific DNA by polymerase chain reaction.
Korean Journal of Perinatology 1993;4(3):391-400
No abstract available.
DNA*
;
Polymerase Chain Reaction*
5.Rapid determination of fetal Y-chromosome with polymerase chain reaction.
Sung Ho KANG ; Kyu Byung JUNG ; Ho Won HAN ; Young Chul KIM ; Sung Il NOH ; Ki Suk OH ; In Kwon HAN ; In Gul MOON
Korean Journal of Obstetrics and Gynecology 1993;36(3):321-325
No abstract available.
Polymerase Chain Reaction*
6.Determination of sex by polymerase chain reaction (I).
Sang Hun CHA ; Tai Ho CHO ; Yong Sang SONG ; Hyo Pyo LEE
Korean Journal of Obstetrics and Gynecology 1991;34(11):1568-1573
No abstract available.
Polymerase Chain Reaction*
7.Some application of PCR in microbiology
Journal of Practical Medicine 2002;435(11):25-27
In microbiology, PCR was applied very early and widely step by step to diagnose the etiology of the infection. Especially in case of the culture of microorganism was unsuccessfully implemented or is very difficult or patient used the antibiotic before admission because PCR can be implemented in the dead microorganism. PCR contributes to verify more correctly.
microbiology
;
Polymerase Chain Reaction
8.Sequencing of Flic genes of Salmonella typhi, S. paratyphi A, S. paratyphi B, S. paratyphi C, and S. typhimurium and application for PCR to differentiate them
Journal of Preventive Medicine 2005;15(5):36-41
All Flic genes of Salmonella typhi, S. paratyphi A, S. paratyphi B, S. paratyphi C, and S. typhimurium code for phase 1 of H antigen (H:1) (d, a, b, c and i antigen respectively). The genes were sequenced on Sanger's principle by automatic sequenser (ABI, 3100 Avant, Genetic Analyser). The primer pairs for the Salmonella species were designed on the basis of the collected sequences. The results showed that PCR with these primers can clearly differentiate the five Salmonella strains, especially between S. paratyphi B and S. typhimurium.
Polymerase Chain Reaction
;
Salmonella
9.Detection of human papillomaviruses in cervical interepithelial neoplasia and invasive carcinoma by in situ polymerase chain reaction.
Joon Cheol PARK ; Tae Sang KIM ; Dong Ja KIM ; Han Ik BAE ; Jeong Ran KIM
Korean Journal of Obstetrics and Gynecology 2000;43(10):1738-1743
No abstract available.
Humans*
;
Polymerase Chain Reaction*
10.Identification of Gastrodia elata and its hybrid by polymerase chain reaction.
Hui LI ; Run QIAN ; Na TIAN ; Yang-Hua LI ; Chao JIANG ; Yuan YUAN ; Lu-Qi HUANG
China Journal of Chinese Materia Medica 2020;45(15):3666-3671
Gastrodia elata is a kind of traditional Chinese medicinal materials and has good medicinal value. G. elata is divided into five varieties, which includes G. elata f. elata(proto variant), G. elata f. glauca, G. elata f. viridis, G. elata f. flavid and G. elata f. alba. Among them, G. elata f. elata and G. elata f. glauca have excellent characteristics and higher contents of gastrodin and polysaccharides. The hybrid of G. elata f. elata and G. elata f. glauca is present in markets, but the characteristics between hybrid and parent are not obvious and distinguished quickly and accurately. The aim of this study is to establish a PCR specific PCR identification method, which can identify G. elata f. elata, G. elata f. glauca and their hybrid. Based on the re-sequencing results of G. elata, we screened for the single nucleotide polymorphism(SNP) variation sites, and designed two pairs of specific primers(W291-F/W291-R and H255-F/H255-R). We further collected G. elata f. elata, G. elata f. glauca and their hybrid samples from different regions, established and optimized PCR method, and investigated and verified their tolerance and applicability. The results showed that when the annealing temperature was 48 ℃ and the number of cycles was 33, 255 bp specific band were obtained from G. elata f. glauca and hybrid by using specific primers W291-F/W291-R. When the annealing temperature was 51 ℃ and the number of cycles was 33, 291 bp specific band were obtained from G. elata f. elata and hybrid by using specific primers H255-F/H255-R. Our method could be used as a promising method to identify G. elata f. elata, G. elata f. glauca and their hybrid.
Gastrodia
;
Polymerase Chain Reaction
Result Analysis
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