Erythrocyte Sedimentation Rate ; Viscosity ; Plasma Proteins ; Plasma ; Fibrinogen

PURPOSE: The aim of this study was to evaluate the clinical efficiency of the subepithelial connective tissue graft (SCTG) with and without plasma rich in growth factor (PRGF) in the treatment of gingival recessions. METHODS: Twenty bilateral buccal gingival Miller's Class I and II recessions were selected. Ten of the recessions were treated with SCTG and PRGF (test group). The rest ten of the recessions were treated with SCTG (control group). The clinical parameters including recession depth (RD), percentage of root coverage (RC), mucogingival junction (MGJ) position, clinical attachment level (CAL), and probing depth (PD) were measured at the baseline, and 1 and 3 months later. The data were analyzed using the Wilcoxon signed rank and Mann-Whitney U tests. RESULTS: After 3 months, both groups showed a significant improvement in all of the mentioned criteria except PD. Although the amount of improvement was better in the SCTG+PRGF group than the SCTG only group, this difference was not statistically significant. The mean RC was 70.85+/-12.57 in the test group and 75.83+/-24.68 in the control group. CONCLUSIONS: Both SCTG+PRGF and SCTG only result in favorable clinical outcomes, but the added benefit of PRGF is not evident.
Connective Tissue ; Intercellular Signaling Peptides and Proteins ; Plasma ; Transplants

Thawing fresh frozen plasma(FFP) by waterbath(WB) requires about 30 minutes, which is too slow in emergency situations and carries the risk of bacterial contamination of FFP. To solve these problems, a new thawing method using a microwave oven(MWO) has been developed. Twenty units of equally divided plasma from 10 units of plasma were frozen, stored at -55 degrees C, and thawed in parallel using microwave oven or waterbath. Coagulation factors, plasma proteins and thawing time were measured. Except for antithrombin III(MWO: 85.2+/-6.94%, WB : 90.8+/-9.14%, p<0.05), no significant differences were observed in the 18 other coagulation parameters and the plasma proteins studied. Mean thawing time by MWO was 5.9 minutes per 1 unit, 10.4 minutes per 2 units and 12.5 minutes per 3 units; by WB, it was 19.0, 20.0 and 22.0 minutes, respectively. In conclusion, FFP can be thawed faster using a microwave oven than using 37 degrees C waterbath and the thawed plasma proteins were generally equivalent to those of FFP thawed by waterbath.
Blood Coagulation Factors ; Blood Proteins ; Emergencies ; Microwaves* ; Plasma*

No abstract available.
Dopamine Plasma Membrane Transport Proteins* ; Dopamine* ; Humans ; Parkinsonian Disorders*

No abstract available.
Dopamine Plasma Membrane Transport Proteins ; Dopamine ; Electrons ; Positron-Emission Tomography

No abstract available.
Dopamine Plasma Membrane Transport Proteins ; Dopamine ; Methylphenidate ; Parkinsonian Disorders

Antidepressive Agents/therapeutic use* ; Serotonin ; Serotonin Plasma Membrane Transport Proteins

Bezoars are persistent concretions of indigestible material that are usually found in the stomach. With the significant development of endoscopic techniques, many authors have reported the removal of bezoars using methods such as endoscopic forceps, snares, electrohydraulic lithotripsy, laser. However, there are no reports of using argon plasma to remove a bezoar in Korea. Argon plasma coagulation is a non-contact electrosurgical technique, which is an inexpensive, easily learned, and effective method in gastrointestinal endoscopy. In addition, this method is associated with a decreased risk of perforation and tissue damage by maintaining a controllable depth of coagulation. We report a 71-year-old man with a 11x11x8 cm sized huge gastric phytobezoar found by endoscopy. The bezoar was broken into pieces using the argon plasma coagulator. Endoscopic forceps and a basket were then used to crush and extract its fragments. The bezoar was removed safely without any complications. We report this case with a review of the relevant literature.
Aged ; Argon Plasma Coagulation ; Argon* ; Bezoars ; Endoscopes* ; Endoscopy ; Endoscopy, Gastrointestinal ; Humans ; Korea ; Lithotripsy, Laser ; Plasma* ; SNARE Proteins ; Stomach ; Surgical Instruments

Rosai-Dorfman disease (RDD) can present in any anatomic site, but breast involvement is rarely reported. Recently, a relationship between RDD and IgG4-related sclerosing disease has been suggested. Here we report another case of RDD with overlapping features of IgG4-related sclerosing disease occurring in a right breast of a 62-year-old female. On microscopic examination, the mass demonstrated a characteristic zonal pattern of proliferation of large polygonal histiocytes and lymphoplasma cells with stromal fibrosis. Emperipolesis was observed in histiocytes with abundant cytoplasm, which showed immunoreactivity for S-100 protein and CD68; the diagnosis of RDD was made. Sheets of plasma cells in the fibrotic stroma demonstrated positive reactions for IgG and IgG4. The mean count of IgG4-positive plasma cells was 100.2/high power field, and the ratio of IgG4/IgG was 56.7%. Additional findings of stromal fibrosis and obliteration of preexisting breast lobules suggested overlapping features with IgG4-related sclerosing disease.
Breast ; Cytoplasm ; Emperipolesis ; Female ; Fibrosis ; Fluconazole ; Histiocytes ; Histiocytosis ; Histiocytosis, Sinus ; Humans ; Immunoglobulin G ; Middle Aged ; Plasma ; Plasma Cells ; S100 Proteins

BACKGROUND: Platelet-rich plasma (PRP) has been advocated as a way to introduce increased concentrations of growth factors and other bioactive molecules to injured tissues in an attempt to optimize the local healing environment. Many methods for PRP preparation have been introduced. Despite variations in the volume of whole blood taken and the efficacy of the platelet concentration, the main objective of PRP preparation is to obtain sufficient platelet concentration in the finally processed autologous plasma. We have been making our own internal primitive PRP preparation, which is safe and aseptic, using simple tubes and a centrifugal separator at the outpatient department base. METHODS: Twenty cc of whole blood was collected and 10 cc of blood was added to each of two bottles, followed by addition of 1.5 cc adenosine-citrate-dextrose-acid solution to each bottle. Then, centrifugal separation was performed at 4,000 RPM for 15 minutes. Then, the buffy coat layer was aspirated using a 10 cc syringe equipped with a spinal needle. Platelet activation was initiated by addition of CaCl2 and botropase. RESULTS: We were successful in attaining PRP, which was three folds and six folds concentrated compared with the initial platelet count of whole blood. CONCLUSIONS: Our protocol is economical and only requires a few simple procedures for preparation of PRP. We expect the protocol to be applied to clinical trials without significant cost of time and money.
Blood Platelets ; Humans ; Intercellular Signaling Peptides and Proteins ; Needles ; Outpatients ; Plasma ; Platelet Activation ; Platelet Count ; Platelet-Rich Plasma* ; Syringes