A comparative study on the histological and morphological characteristics and the intrinsic quality of the roots of Huabei Qianhu(Peucedanum harry-smithii),Shaomao Bei Qiau hun (P.harry-smithii var.subglabrum ) and Baihua Qianhu (P.praeruptorum ) was carried out. Results revcaled that the former two Qianhu produced in Gansu are similar to Baihua Qian hu in their main ingredients.Thus the two Qianhu are worthy for further research and develo pm ent.At the same time, it was observed that Baihua Qianhu Produced in Gansu is of iuferior quality and the content of EtOH extraet of its root is slightly lower than that from elsewhere in China.

Investigation on the plant origin and Pharmacognosy of Aiye grown in Gansu revealed that 12 species of the Artemisia genus were used either as or adultered with Aiye, instead of the genuine drug. Morphological and histological indentifications were presented to distinguish the faked drug.

Objective With fat-soluble ingredients and water-soluble components as indicators,to investigate the processing technology of wine Gansu Salvia by orthogonal test.Method With fat-soluble ingredients and water-soluble components determined by HPLC,the processing technology of wine Gansu Salvia was optimized by orthogonal test.Result The transfer rate of fat-soluble ingredients with water extraction was 2.4%,with little significance,while it had a significant impact on salvianolic acid B.The best process condition was 10% yellow rice wine,added 20% wine,fried in 80 ℃ for 4 min.Conclusion It was reasonable to choose the best process condition for wine Gansu Salvia.

Objective:To analyze and compare the volatile oil in the leaves of Juniperus formosana Hayata from Yuzhong and Lu-qu. Methods:The volatile oil from Juniperus formosana Hayata was extracted by steam distillation, and then identified by gas chroma-tography-mass spectrometry ( GC-MS) . Results:The average yield of the volatile oil from the leaves of Juniperus formosana Hayata from Yuzhong and Lugu was 0. 21% and 1. 45%(w/w), respectively. Between the two areas of the volatile oil from the leaves of Juniperas formsana, totally 40 peaks were separated from the volatile oil from Gansu with 38 ones (95%) were identified. Totally 51 peaks were separated from the volatile oil from Xizang with 47 ones (92%) were identified. The maln constituents in the former volatile oil wereα-pinene(44.92%),l-caryophyllene(9.23%),( -)-isoledene(6. 50%),a-caryophyllene(5.60%),myrcene(4.54%) and d-cadinene(3. 37%), and those in the latter were di-epi-cedrene(31. 87%),cyclohexene(15. 28%),γ-elemene(10. 05%), lanceol (5. 80%) and α-pinene(5. 79%). Conclusion:The constituents in the volatile oil in the leaves of Juniperus formosana Hayata from the two different regions are various, and the differences in the yield and compounds are notable. The method provides reference for the establishment of quality standard for Juniperus. formosana Hayata.

Objective:To establish a method to determine the content of chlorogenic acid and rutin in nettle to assess the quality of nettle.Methods:An HPLC method was used with the following chromatographic conditions:CAPCELL PAK C18 column (250 mm × 4.6 mm, 5 μm) was used with the mixture of 0.4%phosphoric acid and methanol as the mobile phase with gradient elution , the de-tection wavelength was set at 340 nm, the flow rate was 1.0 ml· min-1 and the sample size was 10μl.Results:Chlorogenic acid with-in the range of 2.360-23.600μg · ml-1 showed a good linear relationship (r=0.999 9), and rutin within the range of 6.208-62.080μg · ml-1 showed a good linear relationship (r=0.999 9).The recovery of rutin and chlorogenic acid was 99.20% and 100.40%with RSD of 1.2%and 1.1%(n=6), respectively.Conclusion:The method is fast, effective, simple, accurate and reproducible , which can be used to quantitatively analyze chlorogenic acid and rutin in nettle .

AIM: To analyze the chemical constituents of essential oil in Ephedra Sinica Stapf from Gansu Province. METHODS: The essential oil in Ephedra Sinica Stapf was extracted by steam distillation and analyzed by gas chromatography-mass spectrometry(GC-MS). RESULTS: 71 Compounds were identified from Ephedra Sinica Stapf of 3 different sources(Minqin,Zhenyuan and Gulang County),and contained 28 same components such as tetramethylpyrazine、linalool、?-terpineol、4-terpineol、d-?-terpineol、hexadecanoic acid and so on.The contents of d-?terpineol and hexadecanoic acid in Ephedra Sinica Stapf were the highest. CONCLUSION: Essential oils in Ephedra Sinica Stapf from Gansu Province do not contain 1-?-terpineol.

Objective:To establish an HPLC method for the determination of calycosin-7-glucoside, genistein, formononetin and medicarpin in Radix Hedysari. Methods: The sample was refluxed with methanol in a water bath. The HPLC was performed on an SunFire C18 column(250 mm × 4. 6 mm,5 μm) with acetonitrile-0. 2% H3 PO4 as the mobile phase by gradient elution. The flow rate was 1. 0 ml·min-1 and the column temperature was at 35℃. The detection wavelength was 240 nm and the sample size was 20 μl. Results:The linear range of calycosin-7-glucoside was 0.035-1.042 μg(r=0.999 6), that of genistein was 0.027-0.821 μg(r=0. 999 7), that of formononetin was 0. 031-0. 941 μg(r=0. 999 9) and that of medicarpin was 0. 025-0. 745 μg(r=0. 999 6). The average recovery of calycosin-7-glucoside, genistein, formononetin and medicarpin was 100. 32%(RSD=1. 87%), 99. 3%(RSD=1. 76%), 100. 5%(RSD=1. 48%) and 99. 2%(RSD=1. 45%)(n=6), respectively. Conclusion:The method shows short analyt-ic time, good stability and promising operation accuracy, which provides the reference for the quality control of Radix Hedysari.