Objective:To analyze the incidence trend, current status and survival period of young patients with lung cancer aged from 15 to 44 years in Shanghai between 2002 to 2005. Methods:All the data were collected from the database of registration and ma-nagement system of diagnosed cancer cases in Shanghai city organized by Shanghai Municipal Center for Disease Control & Prevention. The incidence trend was analyzed by using APC(annual percent change)model. The survival period and median survival time were calculated by using SPSS 12.0 software and life table method. The univariate and multivariate analyses were analyzed by using Kaplan-Meier and COX regression model, respectively.Results:A total of 972 young patients with lung cancer who aged from 15 to 44 years were diagnosed in Shanghai between 2002 and 2005, accounting for 3.09% of the whole percentage of adenocarcinoma in young patients was higher than that in whole lung cancer population at the same period, while the TNM stage was not significantly different between male and female young patients. Multivariate analysis demonstrated that gender, pathological classification and TNM stage were independent prognostic factors for young patients with lung cancer. Conclusion:The lung cancer had different characteristics in young patients compared with that in whole lung cancer population in Shanghai city. It was necessary to further investigate the biologic behavior of lung cancer in young patients.

Objective To prepare the supersaturation self-emulsifying drug delivery system(S-SEDDS) containing silymarin and to evaluate its basic properties.Methods With the time of self-emulsifying,the consequence of color visual examination and particle size as parameters,the optimum formulations of silymarin SSEDDS were screened by solubility test,compatibility tests and pseudo ternary phase diagrams.The silymarin concentration was determined by HPLC.The in vitro dissolution characteristics of silymarin S-SEDDS were investigated with silymarin SEDDS as control.Results The optimum silymarin S-SEDDS was composed of medium chain triglycerides(MCT) 40%,Cremophor RH40(ethoxylated hydrogenatedcastor oil) 48%,Labrasol 12%.The time of self-emulsifying was less than 3 min,the average particle diameter was 49.6 nm,the adding amount of hydroxypropyl methylcellulose(HPMC) was 50 mg/g,and the average content of silymarin was 39.3 mg/g.The in vitro dissolution test of silymarin S-SEDDS showed that the presence of a small amount of cellulosic polymer effectively sustained a metastable supersaturated state by retarding precipitation kinetics.Conclusion The designed formulation of silymarin S-SEDDS is reasonable and provides a strong foundation for further development of new preparations.

Background and purpose:Head and neck cancer is common worldwide. This article aimed to describe the patterns of incidence of head and neck cancer and their changes in Shanghai from 2003 to 2012, in order to provide reference for prevention programs, research and control strategies on cancer.Methods:The data of lip, oral cavity and pharynx cancer cases were collected by the Shanghai Cancer Registry. The distributions of Shanghai lip, oral cavity and pharynx cancer incidences from 2003 to 2012 were described. The patterns were described according to gender, age, basis of diagnosis, histological type, diagnostic stage in detail. We compared incidences of recent 5 years (from 2008 to 2012) with those of previous 5 years (from 2003 to 2007).Results:On average from 2003 to 2012, 1105 new cases of head and neck cancer were diagnosed in Shanghai each year, with 2.08% among the total cancer cases. The crude rate was 8.01 per 100000 and the age-standardized rate was 4.45 per 100000. Nasopharyngeal cancer was the major subtype of the head and neck cancer, with 50.81% among the total head and neck cases. The crude and age-standardized rates among males were higher than those among females. The histologically verified percentage was 85.77%. The squamous carcinoma was the major histological type, with 57.58% among the total cases. The age-stan-dardized rate of nasopharyngeal cancer was in decline.Conclusion:The incidence of head and neck cancer was low in Shanghai during the past 10 years. Male cases were more than female cases. The major histological type was squamous carcinoma. Half of new cases were nasopharyngeal cancer which appeared to affect patients at a relatively young age. Patients with nasopharyngeal cancer were diagnosed at relatively advanced stages.

Background and purpose:Incidence and mortality of gastric cancer have increased gradually in China. Population-based cancer survival rate can relfect improvements in cancer diagnosis and treatment, as well as provide evidence for gastric cancer prevention and control.Methods:Data from gastric cancer incidence and follow-up between 2002 and 2006 were collected by the Shanghai Cancer Registry. Life table and EdererⅡ were used to calculate observed survival (OS) and relative survival (RS).Results:A total number of 28 243 gastric cancer cases were documented between 2002 and 2006 in Shanghai. The 5-year OS and RS of gastric cancer were 32.15% and 45.81%, respectively. Survival rates of gastric cancer varied by age, residence, diagnostic stage, and tumor histological type, but not by gender. Patients aged 35-44 years who were diagnosed at stageⅠ or with tubular adenocarcinoma, had the highest survival rates. Moreover, patients in rural areas had higher 5-year OS than those in urban areas. During the period 2002-2006,both 5-year OS and RS of gastric cancer increased dramatically, as compared with 1972-1976. Conclusion:Survival rates of gastric cancer varied by age, residence, diagnostic stage, and tumor histological type. The 5-year OS and RS signiifcantly increased in Shanghai over the past three decades, but are still far below developed countries. Early diagnosis of gastric cancer would be an effective strategy to improve survival and prognosis of patients with gastric cancer.

Background and purpose:The incidence and mortality of colorectal cancer increased gradually in China, cancer survival rate plays an important role in guiding cancer prevention and treatment.Methods:Data of colorectal cancer cases diagnosed during 2002-2006, follow-up information and death report were from Shanghai Cancer Registry, Life Table and EdererⅡ were used to calculate observed survival (OS) and relative survival (RS), the related demographic characteristics and status were also analyzed to relfect the survival situations of the colorectal cancer survivors in major areas in Shanghai.Results:In this study, 16 682 colon cancer cases and 11 906 rectum cancer cases were included in analysis, 5-year OS rates for colon cancer and rectum cancer were 48.84% and 51.65%, RS rates were 70.50% and 71.31%. Signiifcant difference in survival had been found among the various diagnostic stages, the survival rate in stageⅠ was much higher than those in stageⅢ and stageⅣ. The survival of colorectal cancer patients with different gender, age, tumor histological and residential areas were also found with differences, female was higher than male, age ranged from >44-54 years had the highest survival rates than other age groups. The survival of epithelial tumors was higher than other histologic types in colon cancer, and the survival of non-epithelial tumors was higher than other histologic types in rectum cancer. Over the past 3 decades, the 5-year OS increased dramatically in Shanghai. Conclusion:The level of survival of colorectal cancer in Shanghai is similar with it in developed counties. Colorectal cancer survival rate with different population characteristics may provide suggestions and evidence for further improvement of early screening, diagnosis and treatment.

OBJECTIVETo understand the incidence trend of malignant tumors in children aged <15 years in Shanghai.

METHODSThe <15 years old children diagnosed with cancer in Shanghai between 2009 and 2011 were included in this study. The types of malignant tumors were classified according to International Classification of Childhood Cancer, Version 3 (ICCC-3). The gender and age specific incidences of malignant tumors were analyzed. Software Joinpoint was used to calculate the annual percentage of childhood cancer cases.

RESULTSA total of 460 cases of childhood cancer were diagnosed in Shanghai during 2009-2011, accounting for 0.3% of total cancer cases. The crude incidence was 129.0 per million and the age standardized rate (ASR) was 129.6 per million. The ASR was higher in boys (142.1 per million) than in girls (116.4 per million). The boy to girl ratio was 1.2(95%CI: 1.0-1.5). The incidence was highest in age group <5 years (165.1 per million). The incidences in age groups 5-9 years and 10-14 years were 101.2 per million and 113.9 per million, respectively. Leukemia was the most common cancer in children (n=165, 35.9%, ASR: 47.0 per million), followed by central nervous system tumors (n=91, 19.8%, ASR: 25.6 per million) and lymphomas (n=45, 9.8%, ASR: 12.6 per million). The age and gender specific incidence of malignant tumors and the type specific incidence of malignant tumors in children in Shanghai had no significant changes during the study period.

CONCLUSIONSThe incidence of malignant tumors was higher in boys than in girls in Shanghai. Leukemia, central nervous system tumors and lymphomas were the three most common cancers in children. The overall incidence of malignant tumors in children in Shanghai had no significant changes during 2002-2011.

Adolescent ; Child ; Child, Preschool ; China ; epidemiology ; Female ; Humans ; Incidence ; Male ; Neoplasms ; epidemiology

Objective:To investigate the understanding of cancer pain by medical doctors and its therapeutic status in Shanghai. Methods:We recruited 1 982 medical physicians from 106 secondary and tertiary hospitals in Shanghai city from January to March in 2007. The understanding and the therapeutic status of cancer pain were investigated by conducting a questionnaire survey and the results were statistically analyzed.Rusults:There were 52.3% physicians who believed that they understand the treatment protocol of the Three-step Analgesic Ladder regimen. The rate increased by 21.6 percentage point compared with the results in 1999. The understanding degree of oncologists was significantly better than non-oncologists. There were 16.8% physicians giving the correct answers on the three main principles of the dosage titration in analgesic management. The correct rate of oncologists was significantly higher than that of non-oncologists. Opium with strong analgesic effect had become the first choice for severe cancer pain, and the use of pethidine (Dolantin) for severe cancer pain decreased significantly.The pain was significantly relieved in 74.8% patients. The pain-relieving rate increased by 25.4 percentage point compared with the results in 1999. There were 81.1% medical doctors confirming the effect of pain treatment and among them oncologists were more optimistic to therapeutic outcome than others. There were 93.3% physicians who agreed that it was necessary to carry out the standardized training of cancer pain treatment and the requirement of non-oncologists were much emergent. The over-strict controls by the hospitals and the regulations were the main obstacles for using analgesic drugs.Conclusion:The understanding of medical doctors and the diagnostic and therapeutic levels of cancer pain were improved significantly compared with those in 1999. But more training of cancer pain treatment are still required to publicize the standardized therapy and overcome the obstacles of using analgesic drug.

Background and purpose:China is a high incidence area of liver cancer. The latest monitoring data in Shanghai show that liver cancer is one of the most common cancers with very high disease burden. This study aimed to describe and analyze the population-based survival rates of patients with liver cancer in Shanghai.Methods:Data of liver cancer cases diagnosed between 2002 and 2006, including follow-up information and death report, were collected from Shanghai Cancer Registry. Life table and Ederer Ⅱ were used to calculate observed survival (OS) and relative survival (RS). The related demographic characteristics and status were also analyzed to relfect the survival situations of the liver cancer survivors in major areas of Shanghai.Results:In this study, 20 702 liver cancer cases were included in analysis. Five-year OS rate for liver cancer was 11.72%, whereas RS rate was 15.45%. The OS of male liver cancer patients was higher than that of female patients. Patients whose age ranged from 0-34 years had the highest survival rates than patients from other age groups. The survival of patients with hepatocellular carcinoma was higher than that of patients with other histologic types of liver cancer. Signiifcant difference in survival had also been found among patients with various stages of liver cancer. The survival rate of patients with stageⅠliver cancer was much higher than that of patients with stageⅢ andⅣ liver cancers. There was no signiifcant difference in the survival of liver cancer patients between urban and rural residents. Over the past 3 decades, the 5-year OS increased dramatically in Shanghai. Conclusion:The survival of patients with liver cancer in Shanghai is improved signiifcantly. The prognosis is poor compared with other common malignant tumors. It is necessary to strengthen the risk factors and high-risk population control and intervention in the future.

Objective:To investigate the effects of miRNA-499a-5p targeting CD38 on cardiomyocyte injury induced by hydrogen peroxide (H 2O 2) and the possible mechanism. Methods:H9c2 cardiomyocytes were cultured in vitro and induced with H 2O 2 to establish the cardiomyocyte injury model. Four groups including control, H 2O 2, H 2O 2+ negative control (NC) and H 2O 2+ miRNA-499a-5p groups were set up. Real-time quantitative PCR (RT-qPCR) and Western blot were used to detect the expression of miRNA-499a-5p and CD38 in cardiomyocytes, respectively. MTT assay was used to measure the survival rates of cardiomyocytes. The levels of reactive oxygen species (ROS) and the activity of lactate dehydrogenase (LDH) were analyzed with test kits. Annexin V-FITC/PI double staining method was used to detect the apoptosis rates of cardiomyocytes. Western blot was used to detect the expression of B-cell lymphoma/leukemia-2 (Bcl-2), Bcl-2-related X protein (Bax), phosphoinositide 3-kinase (PI3K), phosphorylated PI3K (p-PI3K), protein kinase B (Akt) and phosphorylated Akt (p-Akt). Dual luciferase reporter assay was used to detect the targeting relationship between miRNA-499a-5p and CD38. Results:Compared with the control group, the expression of miRNA-499a-5p, Bcl-2, p-PI3K and p-Akt and the survival rate of cardiomyocytes were significantly reduced in the H 2O 2 group ( P<0.05), while the expression of CD38 and Bax, ROS level, LDH activity and the apoptosis rate were significantly increased ( P<0.05). Compared with the H 2O 2 group, the expression of miRNA-499a-5p, Bcl-2, p-PI3K and p-Akt and the survival rate of cardiomyocytes were significantly increased in the H 2O 2+ miRNA-499a-5p group ( P<0.05), while the expression of CD38 and Bax, ROS level, LDH activity and the apoptosis rate were significantly decreased ( P<0.05). Dual luciferase reporter assay confirmed that CD38 was a target gene of miRNA-499a-5p. Conclusions:miRNA-499a-5p could alleviate H 2O 2-induced cardiomyocyte injury by inhibiting the expression of CD38 and the PI3K/Akt signaling pathway might be involved.

Objective: To analyze the patterns of female breast cancer and their incidence in Shanghai during 2003-2012, and to compare them with international data. Methods: The data of female breast cancer cases diagnosed between 2003 and 2012 were collected from the Shanghai Cancer Registry. The record of cancer cases included the patient's name, gender, date of birth, permanent and residential address, diagnosis site or name, histological type, date of first diagnosis, diagnostic basis and diagnostic stage, etc. Data source of cancer incidence of World, Developed Countries, Developing Countries, East Asia and China in 2012 sourced from GLOBOCAN 2012 database of the WHO International Agency for Research on Cancer (IARC); and the data of USA during 2008-2012 was collected from SEER 18 database from Surveillance, Epidemiology, and End Results Program of the National Cancer Institute (NCI). Incidence indicators of Shanghai female breast cancer were calculated, including the average cases, median age, average age, proportion, crude rate, age-specific rate and age-standardized rate. Results: During 2003-2012, 41 991 new cases of female breast cancer were diagnosed in Shanghai, with an average of 4 199 cases per year, accounting for 17.23% among total new female cancer cases, ranking first in female cancer incidence in Shanghai during 2003-2012, with a crude incidence rate of 60.90 per 100 000 and an age-standardized incidence rate of 33.90 per 100 000. During 2008-2012, an average 4 528 new cases of female breast cancer was diagnosed per year in Shanghai, with 16.97% among total female cancer cases. The median age was 56 years old and the mean age was 57.73 years old. The crude rate was 64.23 per 100 000 and the age-standardized rate was 35.00 per 100 0000, which was higher than those of China (22.1 per 100 000), Eastern Areas (27.0 per 100 000) and Developing Counties (31.3 per 100 000), and lower than that of World (43.1 per 100 000), and Yellow Race Population of USA (68.77 per 100 000). During 2003-2007, on average, 3 869 new cases of female breast cancer were diagnosed per year in Shanghai, accounting for 17.54% of total female cancer cases. The median age was 53 years old and the mean age was 56.44 years old. The crude rate was 57.42 per 100 000 and the age-standardized rate was 32.69 per 100 000. There was a continuing peak stage spanned 25 years old from 50 to 74 years old, during 2008-2012. Compared with those of 2003-2007, the beginning age group of the continuing peak stage was delayed for 5 years old and the ending age group was delayed for 10 years old. Conclusion The age-standardized incidence rate in Shanghai was higher than those of China, Eastern Areas and Developing Counties, and lower than that of World, and that of Yellow Race Population of USA; Compared with 2003-2007, the crude rate and age-standardized rate increased, the median age, mean age and the beginning and ending age group of the continuing peak stage were delayed.