Objective To analyze the composing principles of herbs in prescriptions for treating nervous urinary frequency of children by using traditional Chinese medicine. Methods The prescriptions for treating nervous urinary frequency of children were searched in CNKI, VIP, Wanfang database to establish relevant database, and the composing principles were analyzed by using revised mutual information, complex system entropy cluster, and unsupervised hierarchical clustering in the TCM inheritance system after data entry. Results Based on the analysis of 117 entries in 63 cases of prescriptions, the frequency of 32 entries was more than 5 times;22 combinations contained 2 to 3 entries, including 10 entries in total 11 medicine association rules and their confidence coefficient were obtained, which leaded to 14 new core combinations and 7 new prescriptions. Conclusion Nervous urinary frequency of children is mainly treated with the tonification by Chinese herbal compound, in particular with the herbs which can nourish kidney and reduce urination. The new prescriptions were generated by TCM inheritance system, which need verification through clinical trials.

HCMV infection can cause hepatopathy,pneumonia,diarrhea,thrombocytopenic purpura,and sensorineural hearing loss(SNHL),with high morbility and mortality.pp65(phosphoprotein 65),a major constitutive protein of virion,is closely associated with viral gene expression,immune evasion and cell metabolism.pp65 antigenemia level has positive correlation with clinical symptom,which can guide the preemptive therapyand improve prognosis,is considered as one of the golden standard methods of active cytomegalovirus infection.In this paper,the pp65 protein properties,pathogenesis,application in diagnosis and prevention are reviewed.

The phosphatase of regenerating liver(PRL)families of phosphatases,consisting of PRL-1,PRL-2,and PRL-3,are individually overexpressed in a variety of cancer cell lines and tissues when eom-pared with their normal counterparts.Several recent studies have shown that PRL-3 is expressed at a higher lev-el and at a greater frequency in colorectal cancer with liver metastases compared with primary colorectal tumorsand normal colon tissue.Expression of PRL can enhance metastatic and invasive properties of cells and initiate tumor angiogenesis in experimental mice.However,the exact mechanism and in- teracting proteins of the PRL remain unclear.With further study,PRL-3 may server as an attractive target for therapeutic intervention and marker for colon cancer metastasis.

The variety of primary or secondary pancreatic diseases or injuries in children can cause the changes of pancreatic endocrine function,leading to the structural damage of pancreatic endocrine cells and the dysfunction,which can worsen the disease progression and prognosis.The etiology of pancreatic damage is complex,little is known about the pathogenesis.But the change of pancreatic endocrine function caused by pancreatic damage has clinical significance.Based on current studies and animal experiments,this review covered the etiology and pathogenesis of pancreatic endocrine dysfunction in children with pancreatic damage,which would help promote the cognitive level and the research development in this new field and provide new ideas for the research of pancreatic damage.

Recent researches have shown that gut microbiota play an important role in pathogenesis of immune-related disorders.Type 1 diabetes mellitus,as an autoimmune disease,has received increasing attention,especially in terms of the relationship between the pathogenesis and intestinal microbiota.Change of gut microbiota is an important risk factor of the pathogenesis of type 1 diabetes mellitus which have been confirmed with the animal model studies.This article reviews the role of gut microbiota in the pathogenesis of type 1 diabetes mellitus,and the influence of gut microbiota on host immune response for possible prevention and treatment of type 1 diabetes mellitus in the future.

Objective To study the effect of growth hormone on serum levels of Ghrelin, leptin(LP), insulin like growth factor-1(IGF-1) and insulin-like growth factor binding protein 3(IGFBP3) in children with idiopathic short stature(ISS).Methods 86 patients of ISS who received therapy from September 2013 to September 2015 were selected.According to random number table,those patients were divided into observation group ( n=43) and the control group (n=43).Control group was treated with routine nutrition therapy, while the observation group was added with recombinant human growth hormone.The Ghrelin, LP, IGF-1, IGFBP3 levels and height pre-and post-treatment were compared.Results After treatment, the level of Ghrelin in observation group [ ( 4.85 ±0.41 ) ng/mL ] was lower than that of control group [ ( 5.63 ±0.62 ) ng/mL ] , the level of LP [ ( 6.53 ± 0.78)mg/L] was higher than that of control group[(5.49 ±0.62)mg/L](all P<0.05); the levels of IGF-1[(387.94 ±47.38)ng/mL] and IGFBP3 [(6.74 ±0.73)μg/mL]in the observation group were higher than that of the control group[(243.54 ±36.82)ng/mL,(4.85 ±0.61)μg/mL](all P<0.05); the height in the observation group[(110.17 ±8.12)cm] was higher than that of control group[(114.58 ±9.40)cm](P<0.05).Conclusion Recombinant human growth hormone is well for ISS, which can effectively improve Ghrelin, LP, promote physical growth IGF-1, IGFBP3 levels, promote growth.

?receptors are particularly abundant in the CNS. ? receptors have been shown to exhibit such a wide variety of actions as modulating glutaminergic, dopaminergic and cholinergic neurotransmission. They also play an important role in maintaining cell growth and proliferation, learning and memory. Recent evidence suggests the possible involvement of ? receptors in the pathogenesis of schizophrenia. More researches are expected to supply new targets for treatment and diagnosis.

AIM: To investigate the effects of ?-mercaptoethanol (?-ME) and all-trans rentinal acid (RA) on glial fibrillary acidic protein (GFAP) expression in mesenchymal cells derived from mouse fetal liver in vitro. METHODS: Cells suspension from 14.5-days-old mouse fetal liver were cultured in DMEM/HEPES/F12 supplemented with 20% FCS and mesenchymal cells were acquired after discarding nonadherent cells. The 5th passage cells were induced by ?-ME and RA. The characteristics of treated cells were assayed by immunocytochemistry staining at 5 hours and 5 days after induction. ?-actin as an internal control, GFAP gene expression of mesenchyal cells was detected with semi-quantitative RT-PCR. RESULTS: After being inducted by ?-ME and RA, 80% approximately of the cells exhibited typical neural morphology and about 85% expressed GFAP phenotype. Semi-quantitative RT-PCR showed that mRNA expression of GFAP increased in treated cells versus untreated cells (P

The monosialic acid ganglioside GM1 is rich in brain, which participates in the development process of the nerve system and plays wide neuroprotective roles. This article reviews recent reports about six neuroprotective mechanisms including: activating tyrosine kinase receptor——Trk receptor, modulating Ca 2+ homeostasis in cells, reducing the deposition of A? and its toxic roles in brain, inhibiting the production of reactive oxygen species, enhancing the activity of Na+,K+-ATPase.