The phosphatase of regenerating liver(PRL)families of phosphatases,consisting of PRL-1,PRL-2,and PRL-3,are individually overexpressed in a variety of cancer cell lines and tissues when eom-pared with their normal counterparts.Several recent studies have shown that PRL-3 is expressed at a higher lev-el and at a greater frequency in colorectal cancer with liver metastases compared with primary colorectal tumorsand normal colon tissue.Expression of PRL can enhance metastatic and invasive properties of cells and initiate tumor angiogenesis in experimental mice.However,the exact mechanism and in- teracting proteins of the PRL remain unclear.With further study,PRL-3 may server as an attractive target for therapeutic intervention and marker for colon cancer metastasis.

HCMV infection can cause hepatopathy,pneumonia,diarrhea,thrombocytopenic purpura,and sensorineural hearing loss(SNHL),with high morbility and mortality.pp65(phosphoprotein 65),a major constitutive protein of virion,is closely associated with viral gene expression,immune evasion and cell metabolism.pp65 antigenemia level has positive correlation with clinical symptom,which can guide the preemptive therapyand improve prognosis,is considered as one of the golden standard methods of active cytomegalovirus infection.In this paper,the pp65 protein properties,pathogenesis,application in diagnosis and prevention are reviewed.

The variety of primary or secondary pancreatic diseases or injuries in children can cause the changes of pancreatic endocrine function,leading to the structural damage of pancreatic endocrine cells and the dysfunction,which can worsen the disease progression and prognosis.The etiology of pancreatic damage is complex,little is known about the pathogenesis.But the change of pancreatic endocrine function caused by pancreatic damage has clinical significance.Based on current studies and animal experiments,this review covered the etiology and pathogenesis of pancreatic endocrine dysfunction in children with pancreatic damage,which would help promote the cognitive level and the research development in this new field and provide new ideas for the research of pancreatic damage.

Objective To analyze the composing principles of herbs in prescriptions for treating nervous urinary frequency of children by using traditional Chinese medicine. Methods The prescriptions for treating nervous urinary frequency of children were searched in CNKI, VIP, Wanfang database to establish relevant database, and the composing principles were analyzed by using revised mutual information, complex system entropy cluster, and unsupervised hierarchical clustering in the TCM inheritance system after data entry. Results Based on the analysis of 117 entries in 63 cases of prescriptions, the frequency of 32 entries was more than 5 times;22 combinations contained 2 to 3 entries, including 10 entries in total 11 medicine association rules and their confidence coefficient were obtained, which leaded to 14 new core combinations and 7 new prescriptions. Conclusion Nervous urinary frequency of children is mainly treated with the tonification by Chinese herbal compound, in particular with the herbs which can nourish kidney and reduce urination. The new prescriptions were generated by TCM inheritance system, which need verification through clinical trials.

AIM: To investigate the effects of ?-mercaptoethanol (?-ME) and all-trans rentinal acid (RA) on glial fibrillary acidic protein (GFAP) expression in mesenchymal cells derived from mouse fetal liver in vitro. METHODS: Cells suspension from 14.5-days-old mouse fetal liver were cultured in DMEM/HEPES/F12 supplemented with 20% FCS and mesenchymal cells were acquired after discarding nonadherent cells. The 5th passage cells were induced by ?-ME and RA. The characteristics of treated cells were assayed by immunocytochemistry staining at 5 hours and 5 days after induction. ?-actin as an internal control, GFAP gene expression of mesenchyal cells was detected with semi-quantitative RT-PCR. RESULTS: After being inducted by ?-ME and RA, 80% approximately of the cells exhibited typical neural morphology and about 85% expressed GFAP phenotype. Semi-quantitative RT-PCR showed that mRNA expression of GFAP increased in treated cells versus untreated cells (P

Objective To observe the clinical effect of recombinant human growth hormone(rhGH) in the treatment of Turner syndrome(TS).Methods The clinical data of 40 children with TS who were treated with rhGH were retrospectively analyzed.Results After treatment for 6 months,the GV,IGF-1 levels [(9.4 ± 2.7) cm/a,(433.9 ± 164.9)μg/L] were significantly higher than those before treatment [(2.5 ± 0.4)cm/a,(186.7 ± 7.33) μg/L],the differences were statistically significant(t =45.562 5,5.061 0,all P ＜ 0.05).The treatment dose of rhGH,initial treatment age had no significant relationship with GV,the GH peak before treatment had no significant correlation with GV.There was no obvious adverse reactions in the treatment process.Conclusion For children with TS,rhGH can improve the height,improve the therapeutic effect,reduce the burden of family and society.Low dose of rhGH in the treatment of TS can achieve better curative effect,the initial treatment age and growth rate in children had no obvious relationship,the insulin growth factor is an important index during the treatment,and the changes of insulin growth should be closely monitored,and adjusting the dosage of the drug in order to achieve the best therapeutic effect.

Recent researches have shown that gut microbiota play an important role in pathogenesis of immune-related disorders.Type 1 diabetes mellitus,as an autoimmune disease,has received increasing attention,especially in terms of the relationship between the pathogenesis and intestinal microbiota.Change of gut microbiota is an important risk factor of the pathogenesis of type 1 diabetes mellitus which have been confirmed with the animal model studies.This article reviews the role of gut microbiota in the pathogenesis of type 1 diabetes mellitus,and the influence of gut microbiota on host immune response for possible prevention and treatment of type 1 diabetes mellitus in the future.

Objective To study the effect of growth hormone on serum levels of Ghrelin, leptin(LP), insulin like growth factor-1(IGF-1) and insulin-like growth factor binding protein 3(IGFBP3) in children with idiopathic short stature(ISS).Methods 86 patients of ISS who received therapy from September 2013 to September 2015 were selected.According to random number table,those patients were divided into observation group ( n=43) and the control group (n=43).Control group was treated with routine nutrition therapy, while the observation group was added with recombinant human growth hormone.The Ghrelin, LP, IGF-1, IGFBP3 levels and height pre-and post-treatment were compared.Results After treatment, the level of Ghrelin in observation group [ ( 4.85 ±0.41 ) ng/mL ] was lower than that of control group [ ( 5.63 ±0.62 ) ng/mL ] , the level of LP [ ( 6.53 ± 0.78)mg/L] was higher than that of control group[(5.49 ±0.62)mg/L](all P<0.05); the levels of IGF-1[(387.94 ±47.38)ng/mL] and IGFBP3 [(6.74 ±0.73)μg/mL]in the observation group were higher than that of the control group[(243.54 ±36.82)ng/mL,(4.85 ±0.61)μg/mL](all P<0.05); the height in the observation group[(110.17 ±8.12)cm] was higher than that of control group[(114.58 ±9.40)cm](P<0.05).Conclusion Recombinant human growth hormone is well for ISS, which can effectively improve Ghrelin, LP, promote physical growth IGF-1, IGFBP3 levels, promote growth.

In order to study the rural elderly chronic patient's seeking healthcare behavior and its influencing factors, the paper uses the 2011 national baseline survey data of the “China Health and Retirement Longitudinal Study ( CHARLS)” and the Multinomial Logit model to conduct an empirical analysis .The study results show that:( 1 ) The rural elderly chronic patients are more likely to choose the county-level and above hospitals and village clin-ics instead of the township health centers .(2) The obvious influencing factors in the healthcare seeking behavior in-clude age , educational background , household per capita annual income , chronic disease severity , self-rated health and region.Therefore, the present study suggests that the treatment of chronic diseases does not transfer to the town -ship health centers .The township health centers should improve their chronic medical service abilities , and actively strengthen their functions in chronic diseases prevention and treatment .

Objective To determine the prevalence of sleep disorders and related symptoms of 1 0 years old children in Baoshan Dachang area of Shanghai.Methods From September 201 4 to June 201 5,1 01 6 children at the age of ten were randomly selected and information was collected by using a parent -completed questionnaire to ascer-tain in sleep disorders and related symptoms.Results The prevalence of sleep disturbances in 1 01 6 childrens was 53.5 %,included active 1 6.9%,inattention 1 3.0%,allergic rhinitis 1 2.8%,poor academic performance 1 2.4%, poor learn ablity 1 2.4%,too much sweat at night 8.7%,irritable and slapstick 8.5%,muoth breathing 6.7%,repeat respiratory infection 5.5%,tired easily in the day 5.3%,tousillar hypertrophy 4.7%,growth retardation 3.9%, aweeks at night and sleep terrors 3.4%,anorexia 3.4%,night sleep snoring 2.8%,nasosinusitis 2.6%,bronchial asthma 1 .6%.326 boys in the 502 sleep disorder related symptoms,accounted for 64.9%,21 8 girls in the 51 4 sleep disorder related symptoms,accounted for 42.4%.It was under high prevalence of sleep disorders in male and female children(χ2 =51 .81 5,P <0.01 ).According to BMI,these children were divided into four groups.To compare the incidence of sleep disturbance associated symptoms in children with different BMI.Lean group of children in apnea, aweeks at night and sleep terrors,growth retardation of the occurrence rate was higher,compared to all other groups among children with significant difference.Conclusion At present,it is under high prevalence of sleep disorders and related symptoms at the age 1 0 children in Baoshan Dachang area of Shanghai,which the doctors in Department of ENT and related departments and parents should pay high attention.To develop a good sleep habit of children can help to reduce the occurrence of sleep disorders.