Objective To observe the effect of establishing channels of percutaneous nephrolithotomy on pig′s kidney by B ultra-sound .Methods 32 female Rongchang pigs were divided into four groups :control group ,group F16 ,group F24 ,group F30 ,and 8 pigs in each group .Comparing the time difference of establishing channels in percutaneous nephrolithotomy ,surgery blood loss ,the pressure of pelvis and variance of histology .Results The time of group F16 ,group F24 and group F30 were(95 .00 ± 8 .06)min , (99 .60 ± 5 .55)min and (103 .17 ± 7 .99)min ,there was no significant difference(P>0 .05) .The surgery blood loss of group F16 , group F24 and group F30 were(22 .40 ± 4 .56)mL ,(25 .00 ± 5 .24)mL and (20 .50 ± 7 .87)mL ,there was no significant difference (P>0 .05) .The pressure of pelvis in control group ,group F16 ,group F24 and group F30 were (8 .84 ± 0 .57)cm H2 O ,(23 .54 ± 0 .89)cm H2 O ,(16 .86 ± 1 .06)cm H2 O ,(13 .30 ± 0 .76)cm H2 O ,the difference was statistically significant (P<0 .05) .Four groups had no obvious inflammation cell seeping and hyperplasia of fibrous tissue by HE dyeing .Conclusion The time of establishing channels in percutaneous nephrolithotomy ,surgery blood loss and variance of histology has no obvious relation with the size of the channel .But the pressure of pelvis was affected with different channels and might be related to induce damage of kidney .

Objective:To identify and characterize one Spiroplasma strain (designated as DGKH1) isolated from the blood of a patient with sepsis. Methods:The traditional bacterial culture, staining, morphological observation, physiological and biochemical identification, 16S rRNA gene sequencing, phylogenetic analysis, genome sequencing, and the genome-related index analysis were performed to accurately determine the taxonomic status of the strain DGKH1. Antibiotic susceptibility testing was performed using a specific kit for culturing and testing Ureaplasma urealyticum/ Metamycoplasma hominis. Results:The strain DGKH1 could weakly grow on Columbia blood agar, chocolate agar, and Haemophilus chocolate 2 agar. However, it did not grow in liquid culture medium containing tetracycline (4 μg/ml), doxycycline (1 μg/ml), minocycline (1 μg/ml), josamycin (2 μg/ml), roxithromycin (1 μg/ml), clarithromycin (1 μg/ml), or telithromycin (1 μg/ml). DGKH1 resembling Metamycoplasma hominis formed "fried egg-like colonies" on Mycoplasma solid culture medium. DGKH1 could not be stained by Gram staining. When observed under transmission electron microscopy (TEM) using phosphate buffer as the matrix, the bacteria were spiral-shaped. Results of 16S rRNA gene sequence alignment showed that DGKH1 was highly similar (99.85%) to Spiroplasma eriocheiris CCTCC M 207170 T. However, the urea decomposition test was positive, which was different from all of the known Spiroplasma species. The phylogenetic analysis based on whole genome showed that DGKH1 was clustered in a small branch along with Spiroplasma eriocheiris CCTCC M 207170 T. However, the average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the two strains were 94.14% and 56.00%, respectively, both below the threshold for prokaryotic species identification. Conclusions:DGKH1 represented a potential new species of genus Spiroplasma, closely related to Spiroplasma eriocheiris. Some microbiological characteristics of DGKH1 were similar to Mycoplasmas. However, the natural host and epidemiological data of DGKH1 need to be further studied.