Objective To evaluate the clinical effects of autologous bone marrow transplantation(ABMT) in the treatment of patients with acute leukemia.Methods A retrospective study was accomplished on the ABMT in the treatment of 35 patients with acute leukemia from Oct 1999 to Oct 2004.The median age of the patients was 32.5(9～55) years.Of the 35 patients,26 cases were acute non-lymphocytic leukemia(ANLL) and 9 cases were acute lymphoblastic leukemia(ALL).The patients were pretreated with melphalan(140～180mg/m~2),cyclophosphamide(120mg/kg) and arabinosylcytosin(3g/m~2).Results All patients engrafted successfully.The median follow-up duration was 756(186～1950) days.The 3-year probabilities of disease-free-survival(DFS) for ANLL and ALL were(65.4%?8.9)% and(33.3?13.6)% respectively,and the probabilities of relapse were(30.6?9.2)% and(60.7?25.5)%,respectively.Conclusion To decrease relapse and increase DFS,patients with acute leukemia who have no chance for allogene haemopoietic stem cell transplantation are recommended for ABMT.

Objective:To study of immuno-intervention with prostaglandin E2(PGE_2) on tumor necrsis factor(TNF-?),interleukin 10(IL-10),SOD and NO in rats attacked by vibrio vulnificus.Methods:27 normal rats and 27 hepatopathy rats,they were divided into Vv attacked group,ofloxacin treatment group and combined PGE_2 with ofloxacin protect group(n=9).Results:The result of cytokines detection showed that serum IL-10 and SOD level of combined PGE_2 with ofloxacin protect group was significantly higher than that of ofloxacin treatment group,however,TNF-? and NO level of PGE_2 immuno-protect group were significantly lower than those of ofloxacin treatment group(P

Objective To study on NGF positive cells changes by the action of Danshen and explore the correlation between time and amount of NGF after traumatic brain injury (TBI)in rat.Methods72 rats were randomly divided into 3 groups,including sham group,brain injury and Danshen therapy group.TBI rat models were made by fluid percussion.NGF was determined on the 3rd,7th and 14th day after TBI.ResultsNGF positive cells after traumatic brain injury in brain reached the maximum level at the 3rd day(1.29±0.61),and it decreased since the 7th day(2.03±0.42),and it decreased to the lowest level on the 14th day (0.87±0.23).NGF positive cells after traumatic brain injury treated by Danshen were more than those patients who were treated without Danshen(1.77±0.54,3.35±0.37,0.65±0.21 vs 0.75±0.31,1.58±0.52,0.49±0.36,P＜0.05～0.01).ConclusionsThe expression of NGF was significantly increased in brain damaged tissues.This may be served as a useful marker of diagnosis in time-course of brain leison.Danshen injection can be used to protect brain after traumatic brain injury.

OBJECTIVE:To improve the teaching quality of TCM processing experiment for pharmacy major. METHODS:With the entry point of TCM processing experiment for pharmacy major in our school,it was reformed from the aspects of teaching mode,teaching method,teaching content and assessment method. RESULTS:It changed the original teaching mode,introduced 4 teaching approaches(compulsory experiment elective experiment,open experiment and exploratory experiment),established speci-men bank for common TCM processing and teaching methods (including audio and video),designed contents for basic experi-ment,comprehensive experiment,design experiment and research experiment and established 4 assessment contents(pre-experi-ment,operation,experimental design and experimental test). CONCLUSIONS:According to the reform of teaching method and means of the course,the teaching quality of TCM processing experiment is improved,and the students,innovation and practice abil-ity are developed.