1.The changes of brain natriuretic peptide in patients with myocardial infarction
Pingan CHEN ; Shaonan LI ; Zhen LIU
Chinese Journal of Primary Medicine and Pharmacy 2010;17(2):150-151
Objective To observe the changes of brain natriuretic peptide(BNP)in patients with myocardial infarction,and to assess the clinical value of BNP in the patients with myocardial infarction.Methods BNP was determined in 91 patients with myocardial infarction and in 90 normal cases,and the difference was studied between the area of myocardial infarction and the number of coronary arteries stenosis.Results BNP(ng/L)(155.03 ± 113.45,71.03 ±37.01)and LVEDd(cm)(5.11 ±0.51,4.56 ±0.43)were significantly increased in patients with myocardial infarction than that in normal cases(P<0.01),but EF(%)(50.84 ±7.98,57.09 ±6.29)was lower than that in normal cases(P<0.01).BNP and LVEDd were also significantly higher in patients with wide-anterior myocardial infarction than that in antero-septal,inferior and posterior wall myocardial infarction,but LVEF was lower.Conclusion The BNP level was higher in patients with myocardial infarction,especially in patients with wide-anterior myocardial infarction.BNP was a reliable bio-chemical marker which can evaluate the degree and prognosis of patients with myocardial infarction.
2.Effects of Stoves Improving in Controlling Fluorosis in Coal-burning Endemic Fluorosis Areas in Shaanxi Province from 2001 to 2005
Xiaoli LIU ; Yue LI ; Pingan LI
Journal of Environment and Health 2007;0(12):-
Objective To observe the effect and the influential factors of stoves improving in controlling fluorosis in the coal-burning endemic fluorosis areas. Methods Among the households whose stoves had been improved by 2001 and the improved ones were in use during the whole monitor, the dental fluorosis in children aged 8-12 years (n=318) and the skeletal fluorosis in adults older than 16 years were examined and the fluoride concentration in the indoor air, the corn, chili and the urine of children (n=253) was determined respectively. Results The dental fluorosis rates were 64.79%-87.93%, the skeletal fluorosis rate were 17.95%-21.97%, the fluoride levels in the urine were 2.50-4.03 mg/L, in the indoor air per day were 0.033 7-0.175 3 mg/m3, in chili and corn were 48.13-251.40 mg/kg, 2.06-4.06 mg/kg respectively. The dental fluorosis rate and the index went down significantly year by year from 2001 to 2004. The fluoride content in the chili and the children’s urine decreased in varying degrees from 2001 to 2005. The fluoride levels in the indoor air in 2004 and 2005 were significantly lower than that in 2001 respectively. Conclusion Coal-burning endemic fluorosis is still prevailing in this village. Stoves improving is an effective measure to prevent endemic fluorosis caused by coal-burning.
3.Optimum Selection Processing Study of Rhizoma Atractylodis Macrocephalae Stir-fried with Bran by Orthogonal Design
Quanjiang LONG ; Pingan WU ; Fenglin LIU
Chinese Journal of Information on Traditional Chinese Medicine 2006;0(04):-
Objective To research the best processing method of Rhizoma Atractylodis Macrocephalae stir-fried with bran. Methods L9(34) orthogonal table are decided with three factors:The amount of the adjuvant, heating temperature, heating time. According to the amount of water-soluble extractive, alcohol-soluble extractive and volatile oil, orthogonal design is applied to processing. Results The best processing method is:Rhizoma Atractylodis Macrocephalae were processed by stir-frying with 10 percent of bran for 5 min in a heat of 150 ℃. Conclusion The results can provide basis for establishing the quality standard of this crude drug.
4.Inhibition of hepatitis B surface antigen and hepatitis B virus DNA secretions by hepatitis B immunoglobulin poly(butylcynaoacrylate)nanoparticles in vitro
Zhongtian PENG ; Deming TAN ; Shunling HUANG ; Pingan ZHU ; Fei LIU
Chinese Journal of Infectious Diseases 2009;27(6):330-334
Objective To investigate the inhibitive activities of hepatitis B immunoglobulin(HBIG)poly(butylcynaoacrylate)nanoparticles(HBIG-PBCA-NP)to hepatitis B surface antigen(HBsAg)and hepatitis B virus(HBV)DNA secretions using HBV infected cell model in vitro.Methods HepG 2.2.15 cells were cultured with media containing HBIG-PBCA-NP or HBIG for several days,or cultured with HBIG-PBC-NP and HBIG for 2 days and without HBIG-PBCA-NP and HBIG from day 3.The supernatants at different time points were collected for quantitative detection of HBsAg and HBV DNA.The comparisons between groups were done by variance analysis.Resalts Secretions of HBsAg and HBV DNA in supernatants of HepG2.2.15 cultured with 0.1-10.0 IU/mL of HBIG-PBCA-NP and HBIG were inhibited significantly compared with control group.HBsAg titers and HBV DNA levels in supernatants of HBIG-PBCA-NP group and HBIG group cultured with media without HBIG-PBCA-NP and HBIG kept decreasing at day 5 and 7,then rebounded at day 9 and 11.HBsAg titera in supernatants of 0.1,1.0,5.0 IU/mL HBIG-PBCA-NP group were all significantly different from those in HBIG group at day 9[(31.31±1.98)μg/L vs(40.62±2.99)μg/L,(23.79±1.31)μg/L vs(36.51±2.12)μg/L,(19.91±1.74)μg/L vs(33.03±1.65)μg/L;F=412.24,P<0.01].Couclusion HBIG-PBCA-NP can inhibit secretions of HgsAg and HBV DNA in vitro,which is more effective than HBIG.
5.Effects of ischemia postconditioning in reducing ischemia reperfusion injury in acute limp ischemia
Shaonan LI ; Huifang HUANG ; Guanglian LI ; Zhen LIU ; Pingan CHEN
Chinese Journal of Primary Medicine and Pharmacy 2012;19(15):2241-2243
Objective To study the effects of ischemia postconditioning(IPC) in reducing ischemia-reperfusion injury(IRI) in acute limp ischemia(ALI) and investigate the mechanism.Methods 45 New Zealand rabbits were treated with the method that combined high lipid diets and femoral intima injury by balloon inflation to build up limp atherosclerotic stenosis model,then they were randomly divided into three groups( each group 15 rabbits):control group;IR group and IPC group.Serum creatine phosphate kinase(CK),malondialehyde(MDA),superoxide dismutase (SOD) in three groups were measured before occlusion and 2 hours after sustaining reperfusion.The histological changes of limp skeletal muscle of experimental rabbits were analyzed and TUNEL method was used to access apoptosis of skeletal muscle cells.Results The levels of CK,M DA in IPC group were lower than IR group [ (7.49 ± 0.84)vs (8.19 ± 1.06),P<0.05],[ (3.67 ±0.36) vs (4.06 ±0.55),P <0.05] while SOD level was higher than IR group [ (420.40 ± 30.94 ) vs ( 384.73 ± 44.12),P < 0.05 ] ; The injury of skeletal muscles in I PC group was lighter than IR group and the apoptosis index of skeletal muscle cells was significantly decreased compared with that in IR group[(12.27+2.11)% vs (16.62 ± 1.44)%,P<0.01].Conclusion Applying IPC in acute limp ischemia could alleviate IRI and protect skeletal muscles.The mechanism was associated with oxidation resistance enhancing and the effects in reducing apoptosis of skeletal muscles induced by IRI.
6.Relationship of serum C reactive protein level with serum leptin concentration in type 2 diabetic patients
Yanju HE ; Huimin BI ; Zhenhua LIU ; Dong LI ; Pingan ZHANG
Clinical Medicine of China 2008;24(8):806-808
Objective To explore the association of serum high-sensitivity C reactive protein (hs-CRP) level with leptin in patients with type 2 diabetes mellitns (T2DM). Methods 35 cases of T2DM patients were involved in the study and 16 normal subjects were taken as controls. Fasting serum leptin and CRP were measured .The relationship between them were also analyzed. Results Fasting serum hs-CRP concentration in T2DM group was higher than that in healthy control group, but serum ieptin levels is the same (P > 0.05). Partial correlation analysis demonstrated that fasting hs-CRP level was positively correlated with leptin (r =0.512 ,P =0. 018). Conclusion Fasting serum hs-CRP level is significantly raised in T2DM ,which is associated with leptin. There is chronic inflammatory reaction in T2DM patients,and leptin is possibly involved in inflammatory pathogenesis of T2DM.
7.The predictive value of Aldosterone and PAI-1 to the severity of coronary artery lesions and recent prognosis in patients with Acute coronary syndrome
Shaonan LI ; Guanglian LI ; Zhen LIU ; Xiaoming LEI ; Pingan CHEN
Chinese Journal of Primary Medicine and Pharmacy 2010;17(19):2619-2621
Objective To investigate the predictive value of serum aldosterone and Plasminogen activator inhibitor-1 ( PAI-1 ) levels to the severity of coronary artery lesions and the recent prognosis in acute coronary syndrome (ACS) patients underwent percutaneous coronary intervention(PCI). Methods The study included 160 consecutive patients admitted. All the patients received coronary angiography and were divided into two groups:control group and ACS group. The patients' blood samples were abtained at the root of aortic artery to measure the serum aldosterone and PAI-1 levels. We analyzed their relationship with the severity of coronary artery lesions. The patients were also divided into three groups( high-score group, moderate-score group,low-score group)according to Gensini accumulated points,we analyzed the difference of aldosterone and PAI-1 levels in three groups. The predictive values of aldosterone and PAI-1 to the recent pronosis of ACS patients underwent PCIwere assessed. Results ( 1 )The patients' serum aldosteeone and PAI-1 levels in ACS group were significantly higher than control group( all P <0. 05 ) ;(2)The aldosterone and PAI-1 levels in high-score group were higher than moderate-score group, and moderate-score group was higher than low-score group. (3)The serum aldosterone level in ACS patients was positively correlated with the PAI-1 level ( r = 0.213, all P < 0. 05 ). ( 4 ) Multiple factor Logistic regression analysis indicated that aldosterone, PAI-1 levels were srtong independent predictors for the recent prognosis of ACS patients underwent PCI. Conclusion The aldosterone ,PAI-1 levels in ACS patients were correlated with the severity of coronary artery lesions. Both of them were strong predictors for the recent prognosis of ACS patients underwent PCI.
8.Effect of taurine on the differentiation of 3T3-L1 preadipocytes
Min LIU ; Pingan HU ; Ke CHEN ; Zhaohui MO
Chinese Journal of Tissue Engineering Research 2007;0(12):-
AIM: Some studies found that the glucose uptake ability of diabetes mellitus mice is stronger after feeding with taurine. Whether the taurine can affect adipocytes or not deserves further studies. This article investigates the effects of taurine on the differentiation of 3T3-L1 preadipocytes and detects the mechanics. METHODS: Experiments were performed in the Laboratory Center of Xiangya Third Hospital of Central South University from July to September 2006. ①3T3L1 preadipocytes were provided by Shanghai Cell Bank of China Academy of Sciences. ②3T3-L1 preadipocytes were cultured in high glucose DMEM medium containing fetal bovine serum of 0.10 volume fraction, 108 u/L benzylpenicillin and 80?1010 U/L streptaquaine. After confluence, cells at 5?107 L-1 were incubated in culture flask, and induced with 0.5 mmol/L IBMX, 0.5 mg/L insulin and 1 ?mol/L dexamethasone. The cells in an experimental group were intervened with taurine, whereas these in a control group were not treated with taurine. Oil O staining was used to observe the development of adipose cells. C3T3-L1 preadipocytes were treated with 10,20 mmol/L taurine respectively for 24 and 48 hours. RNA and protein were extracted in the control group and the experimental group. ③Genes related to adipose cells development was examined by RT-PCR and Western-blot. RESULTS: ①The number of cells stained by oil O was less in the experimental group than the control group in 3T3-L1 preadipocytes treated with 10 mmol/L taurine for 14 days. ②After C3T3-L1 preadipocytes were treated with 10,20 mmol/L taurine respectively for 24 and 48 hours, there were no changes in expression of Insig-2 protein, PPAR, Insing-2, adiponectin, adiponectin receptor, GLUT-4, AP-2 mRNA. CONCLUSION: Taurine inhibits the differentiation of 3T3-L1 preadipocytes, but the mechanics still need to be investigated.
9.Research on Pseudomonas Aeruginosa Quinolone-resistant Plasmid Genes
Shike LUO ; Xianhua LIU ; Pingan ZHU ; Chunhui FAN
Journal of Modern Laboratory Medicine 2015;(1):39-41,45
Objective Pseudomonasaeruginosa resistance to quinolones and around symbiotic bacteria resistant plasmids each chromosome metastasis.Methods 481 samples was collected from the Seventh People’s Hospital of Shenzhen since January 2011 to December 2013,and it cultured Pseudomonasaeruginosa 31 cases,susceptibility testing confirmed 15 cases of quin-olone-resistant as the research obj ect,using plasmid transformation,pick up experiments confirmed the presence of the re-sistance plasmid,PCR amplification,gene sequence analysis,and gene sequences surrounding the symbiotic bacteria resistant plasmids as a same.Results The same gene sequence of plasmid was found between drug-resistant strains of Pseudomonas aeruginosa and the surrounding symbiotic bacteria[χ2=1.207,P<0.01].Conclusion Resistance plasmids could be trans-ferred between different species of bacteria.
10.Effects of Fresh Slices on Content of Ferulic Acid in Angelica
Yuefeng LI ; Pingan WU ; Fenglin LIU ; Xingke YAN
Chinese Journal of Information on Traditional Chinese Medicine 2014;(9):90-92,93
Objective To explore effects of fresh slices on quality of angelica;To provide a reliable method for scientific evaluation and the effective control of quality of angelica;To compare the quality of fresh slices and pieces of angelica.Methods Extraction method and the chromatographic conditions were optimized on the basis of 2010 edition of Chinese Pharmacopoeia. Ferulic acid content was determined by HPLC, with a Kromasil C18 column (4.6 mm×250 mm, 5μm) and mobile phase of acetonitrile-0.05% phosphoric acid-water gradient elution at flow rate of 1.0 mL/min, detection wavelength of 316 nm, and the column temperature was 28℃.Results The content of ferulic acid in the fresh slices of angelica was higher than that in angelica pieces. When other external conditions were the same, the content of ferulic acid in the fresh sliced angelica was the highest with 70% methanol extracted for 30 min.Conclusion The method for determination of ferulic acid in angelica is simple, reliable, reproducible, and can be used as an effective way to control quality of angelica.