Objective To discuss the effect of Chinese medicine treatment on quality of life(QOL)of perimenopause syndrome(PMS)patients.Methods The evaluation with WHO QOL-BREF in Chinese version was applied to compare the QOL scores of normal perimenopause females with those of PMS patients.Results The scores of normal perimenopause group were higher than those of PMS group and the difference of scores of all aspects was significant(P

Objective To determine the effect of reducing mA on the diagnostic quality of images and the radiation dose to the lens in patients undergoing paranasal sinus CT. Methods Fifty patients undergoing paranasal sinus CT at 200 mA or 50 mA. The lens dose was measured using thermoluminescent dosemeters [LiF(Mg?Cu?P)]. Image quality was evaluated for six anatomical structures, including middle turbinate, uncinate process, maxillary ostium, ethmoid infundibulum, frontal recess, and cribriform plate. These anatomical structures were assessed as: not seen (0 points), demonstrated but not clearly visualized (1 points), or clearly demonstrated (2 points). Results Mean radiation dose to the lens was significantly reduced from 7.339 mGy (200 mA) to 1.108 mGy (50 mA). No significant difference of image quality between the two mA protocols was found in either rhinosinusitis group or non-rhinosinusitis group. And for rhinosinusitis the image quality showed no significant difference between high-resolution arithmetic and soft arithmetic. Conclusion CT scanning of the sinuses can be performed at greatly reduced mA without loss of diagnostic quality of the images. As far as the rhinosinusitis is concerned, high-resolution arithmetic can not improve the image quality and soft arithmetic is enough.

Background C57BL/6.NOD-Aec1Aec2 mouse is considered to be an idea model for the study of the pathogenesis of Sj(o)gren syndrome,but the cause of dry eye in these mice is unclear.Objective This study was to investigate the histopathological change of the ocular surfaces of C57BL/6.NOD-Aec1Aec2 mice,and to determine whether dry eye is developed spontaneously in these mice.Methods Forty-five clean C57BL/6.NOD-Aec1 Aec2 mice were used as the experiment group and forty-five C57BL/6J mice(both male and female)were used as the control group in this study.Detection of fasting blood-glucose,Schirmer' s test Ⅰ (S Ⅰ t),lissamine green staining and scoring of the corneal and conjunctival epithelium in the mice were performed at the age of 4,8,12,16 and 20weeks.Five mice from each group were sacrificed and their corneas were obtained to measure the central corneal epithelium thickness and to count the number of conjunctival goblet cells.In addition,lymphocyte infiltration in the lacrimal gland of the mice was examined with hematoxylin-eosin staining.The uhrastructure of the corneal epithelial cells and microvilli were assessed by scanning electron microscopy.The use and care of the mice were approved by the Experimental Animal Care Committee of the Third Military Medical University.Results No sign of dry eye was seen in both the 4-week-old C57BL/6.NOD-Aec1Aec2 mice and 4-week-old C57BL/6J mice.The S Ⅰ t values in 8-week-old,12-week-old,16-week-old and 20 week-old mice from the experiment group were (2.7 ±0.9) mm,(2.5 ±0.8) mm,(1.8±0.6) mm and (1.9± 0.1) mm,respectively,showing a significant reduction in comparison with those of the control mice of the same age(all P＜0.01).The amount of lissamine green staining in the C57BL/6.NOD-Aec1Aec2 mice gradually increased with age,showing elevated scores in 12-week-old,16-week-old and 20-week-old mice in the experiment group(all P＜0.01).The central corneal epithelium thicknesses were(20.18±3.75)μm,(17.01 ±5.25) μm,(14.19±5.72) μm and(12.00±3.25) μm in the 8-week-old,12-week-old,16-week-old and 20-week-old C57BL/6.NOD-Aec1Aec2 mice,respectively,which were significantly lower than those of the C57BL/6Jmice of the same age (all P＜0.01).The numbers of conjunctival goblet cells were (8.2±2.4),(6.2±2.1),(6.1 ±2.2) and (4.1 ± 2.0) in the 8-week-old,12-week-old,16-week-old and 20-week-old C57 BL/6.NOD-Aec 1Aec2 mice,respectively,showing a gradual decrease with age and a significant decline in comparison with those of the C57BL/6Jmice of the same age(all P＜0.01).Lymphocyte infiltration in the lacrimal gland and destruction of gland ducts were seen by hematoxylin-eosin staining,and acinar abnormality aggravated with aging.Reduction of corneal epithelial cells and the number of microvilli were distinguished with aging under the scanning electron microscope.The fasting bloodglucose levels of the two groups were both less than 6.0 mmol/L,and no significant difference was found between them at any age(P=0.637,0.610,0.163,0.086,0.938).Conclusions C57BL/6.NOD-Aec1Aec2 mice develop dry eye spontaneously with aging.The course of disease and characteristics of dry eye in C57BL/6.NOD-Aec1Aec2mice is similar to human dry eye.The C57BL/6NOD-Aec1 Aec2 mouse is the perfect model to study the pathogenesis of dry eye.

Objective: To investigate recombinant human insulin-like growth factor-1 (rhIGF-1) on the phosphorylated serine-threonine protein kinase (pAkt) and apoptotic cells in edematous perihematomal brain regions following experimental intracerebral hemorrhage in rats. Methods: A total of 78 healthy male rats were randomly assigned to a false operation group (n =6), a cerebral hemorrhage control group (control group for short, n=36) and a rhIGF-1 intervention group (intervention group for short, n=36). According to 6 time points after giving intervention measures, the rats of the control and intervention groups were randomly redivided into 6 subgroups: 6 h, 12 h, 1 d, 2 d, 4 d, and 7 d subgroups (n =6 in each subgroup). A rat model of acute cerebral hemorrhage was induced by stereotaxic injection of collagenase type IV. RhIGF-1 (50 μg/kg) was injected via tail veins in the intervention group, once a day, until the end of the experiment. An equivalent isotonic saline was injected via tail veins each day in the sham operation and control groups. Samples were taken from each group at a specified time. An immunohistochemical method was used to observe the expression of pAkt around hematomas ; deoxynucleotidyl transferase-mediated terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) technique was used to observe apoptotic cells. Results: Circled digit oneThe neurological deficit score of the rats in the control group and intervention group reached the highest at day 1 or day 2 after procedure, then decreased gradually. The only score in the intervention group at day 7 after procedure was lower than that in the control group. The difference was statistically significant (P <0. 05). Circled digit twoThere were only a few expressions of pAkt positive cells in rats of the false operation group. Except the 6-hour time point, the numbers of pAkt positive cells at the remaining time points in the intervention group were higher than those in the control group. The difference was statistically significant (P < 0. 05). Circled digit threeOnly a few TUNEL positive cells were observed in the false operation group. Except the 6-hour time point, the numbers of TUNEL positive cells at the remaining time points in the intervention group were significantly lower than those in the control group. The difference was statistically significant (P < 0. 05). Conclusion: RhIGF-1 may reduce apoptosis after cerebral hemorrhage and improve the neurological function. Its role may be associated with the activation of pAkt.

OBJECTIVE To explore the value of the continuous quality improvement(CQI) in the quality control of disinfection supply center.METHODS The CQI was applied to every aspect of disinfection supply center.We analyzed and identified the reason of the existence of quality problems,and took CQI measures and the implementaion of quality improvement.RESULTS After 5 years CQI,quality control of disinfection supply center had been remarkably improved.The harmony had increased among staff year by year.The professional knowledge rose from 60% to 100%,the rate of monitoring raised from 70% to 100%,the pass rate of sterilized package raised from 55% to 95%,the satisfaction rate of the relevant sections raised from 85% to 100%.The errors and accidents were eliminated or reduced owing to pay attention to quality control.CONCLUSIONS CQI plays an important role on medical service safety and can effectively improve the medical safety,medical quality and service quality.

One of the strain of bivalent HFRS vaccine, Z37 strain was isolated from Rattus norvegicus and identified as SEO virus by serological test. The M segment cDNA of Hantavirus Z37 strain was obtained by reverse transcripti on and polymerase chain reaction, subsequently cloned into pGEM-T vector. The s equence of positive recombinants was determined by the method of dideoxy chain t ermination, which revealed that the M genomic segment is 3651 nucleotide in len gth with a predicated long open reading frame encoding a protein of 1133 amino acids. Comparison with HNT type (76-118, A9, HV-114 strains) indicated that th ere were 71.8%～72.1% homology at the nucleotide level, 76.2～76.7% homology at the amino acid level. Comparison with SEO type (R22,L99,80-39 strains) showed 95.3～96.1 homology at the nucleotide level, 95.3～99.2% homology at the amino acid level. The results of nucleotide and amino acid comparison indicated that Z 37 strain is SEO viruses in molecular level.

Objective To evaluate the interactive relationship between inflammation detected by single photon emission computed tomography combined with computed tomography (SPECT/CT) and magnetic resonance imaging (MRI) detection of synovitis,bone marrow edema (BME) in the early rheumatoid arthritis (RA) patients.Methods Twenty patients with early RA were included into this study.All patients were diagnosed based on the 2010 ACR/EULAR classification criterion.Patients' unilateral hands (MCP 2-5 and PIP 2-5 joints) were inspected by SPECT/CT (label:99Tcm-MDP) and MRI (3.0 T).SPECT/CT and MRI images were analyzed by two reviewers blinded to other clinical information.Increased bone metabolism of the regional area of interest (ROI) was analyzed and the uptake ratio was calculated.MRI quantitative evaluation was completed by RAMRIS score system.Statistical analysis was performed using SPSS 17.0 software.Student's t-test,analysis of variance (ANOVA),x2 test and post-hoc analysis (Scheffe) were used for comparison of datas,correlation analysis was carried out with Spearman rank correlation test.Results Twent ypatients with a total of 160 joints were examined.MRI examination revealed synovitis (39,24.4％),or BME (24,15.0％) in 63(39.4％) joints.99Tcm-MDP uptake increased in 79(49.4％) joints,and the uptake ratio was higher than normal joints (1.59±0.26 vs 1.15±0.09,t=6.408,P＜0.01).The uptake ratio between the graded synovitis level and RAMRIS synovitis score had a moderate correlation (r=0.535,P=0.001).The uptake ratio of BME level and BME score was well correlated (r=0.765,P=0.001).Conclusion In early RA patients,MRI is sensitive for synovitis and BME.SPECT/CT can detect early abnormal bone metabolism associated with inflammation,which is more sensitive than MRI.SPECT/CT can be used to detect changes of RA at the molecular level.Compared with synovitis,BME is more closely related to bone metabolism changes,which may suggest that BME may play a major role in the bone destruction in RA.However,further studies are needed to verify this hypothesis.

Objective To explore the effect of analgesia management on the analgesic effect and the quality of delivery of labor analgesia. Methods The 80 cases were divided into two groups according to the odd number of labor analgesia, the control group without anesthesia nurse labor analgesia, only the anesthesiologist operation, given routine obstetric care. Anesthesia nursing group for labor analgesia by anesthesiologists and nurse by anesthesia on maternal perinatal period health education and pain management, pain score was compared between the two groups (at full dilatation), mode of delivery, postpartum hemorrhage, neonatal asphyxia. Results The total score of pain in the nursing group was 3.9±0.2, while that of the control group was 8.4±0.8, and the difference was statistically significant (t=-38.5872,P=0.000); anesthesia nursing group cesarean delivery, delivery, were 1 cases, 27 cases, 12 cases; the control group were 9 cases, 21 cases, 10 cases, the difference was statistically significant (χ2=7.31, P<0.05) ; the amount of bleedingof anesthesia nursing group (175.5±42.5) ml; the control group was (226.4±52.5) ml, the difference was statistically significant (t=8.621, P<0.05); the incidence of neonatal asphyxia anesthesia nursing group 1 cases (2.5%); the control group was 3 cases (7.5%), the difference was not statistically significant (χ2=0.263, P>0.05). Conclusions During the perinatal period, the labor management and nursing intervention of the parturient women with labor analgesia are significantly reduced, the cesarean section rate is significantly reduced, and the complication rate is reduced.

In this paper,the author introduced the features of online mendelian inheri-tance in man database,and intensively reviewed its application in teaching and research of medical genetics,thus providing teachers and scientists an updating dictionary.

Objective To develop a modified method of adherent culturing NSCs to replace the traditional suspension culturing method.Methods Neural stem cells(NSCs)were isolated from brain of fetal mouse at 15.5-day fetal age.In culture bottle which had been pre-coated with poly-L-ornithine hydrochloride/fibronectin(PO/FN),the cells were adherently cultured and passaged.The cell morphology was observed under inverted microscope.NSCs and their differentiated cells were identified by immunofluorescence.Results NSCs obtained in this study were successfully adherently cultured and expressed specific markers.Conclusion NSCs are successfully adherently cultured in this study.As compared with the traditional suspension culturing method,adherent culturing is simpler and has lower wastage of passage.It is easy to observe cell morphology and differentiation by this method.It is also helpful to culturing and storage of NSCs.