Objective To analyse the MRI characteristics of the chronic clicking temporomandibular joints(TMJs).Methods 20 TMJs in 10 young chronic clicking individuals (12 clicking TMJs)and 60 TMJs in 30 young healthy volunteers were examined by MRI scanner, and sagittal oblique opened-mouth and closed-mouth position images were observed.Then comparing the MRI findings of the acquired j oints between two groups.Results The ratio of the non-double concave articular disc,slightly high signal intensity in the articular disc, abnormal changes of the condyle,articular fluid,anterior displacement of the articular disc,and increasedDraceangles in the clicking group were significantly higher than those in the control group(P<0.05).The result revealed statistically significant differences.Conclusion The MRI anatomical characteristics can be an indicator for the TMJ anomalys,and provide an obj ective radiological basis for clinical diagnosis and treatment to some extent.

Urid acid is one of the terminal metabolites of human body.More and more attention was paid to its metabolic mechanisms in intestinal tract;however, few studies were seen so far.This study aims to illuminate the metabolic mechanism of uric acid in intestinal tract by two ways:one is the transporters associated with intestinal epithelium, including ABCG2 and SLC2A9, the other is decomposition of uric acid by intestinal flora.We hope this review can provide new insights to decrease blood uric acid and treat urate-related diseases, and also provide a new way to alleviate drug-induced kidney damage.

Low zinc (1.4-1.5 ppm) ration containing saturated fat (coconut oil) was fed to weanling rats, death occurred since second week. In third week, total death was 55%. The survivors manifested severe Zn-deficiency symptoms. When they were given Zn supplement in drinking water (100 ppm Zn), the deficient symptoms disappeared quickly and body weights increased rapidly. When coconut oil was replaced by safflower oil, the rats didn't show any Zn-deficiency symptom in 4 weeks. There was no difference in Zn contents of plasma and hair between Zn-deficient rats and the normals. Adjusting fatty acids composition of dietary fat to contain linoleic acid at 20% of total fatty acids, the rats manifested Zn-deficient symptoms after 2 weeks, and Zn contents of plasma and hair were significantly lower than that of normal rats(P

Zinc deficient rats were injected subcutaneously each day for 3 weeks with 250 ?l evening primrose oil (EPO, containing 10% Y-linolenic acid and 73% linoleic acid) or safflower oil (SFO, containing 77% linoleic acid only) and compared with zinc supplemented group (ZnS) and zinc deficient group (ZnD) without injections. At the end of the experiment, rats were killed under anaesthesia, organ weights, zinc content of kidneys, blood cholesterol and fatty acid compositions were determined. The results showed animals in EPO group had the best recoveries both in deficient symptoms and in body weights,the zinc content of kidneys was the highest, the serum cholesterol level was significantly lower than those of SFO and ZnD groups, but no difference from ZnS group. The total percentage of 22-C and above(?-6)polyunsatured fatty acids was the highest among those four groups. These results indicated that?-linolenic acid was beneficial to zinc deficient rats in growth, zinc utilization and the metabolism of essential fatty acids.

Objective To investigate costimulatory pathway of breast cancer patients, we examined peripheral CD28 positive T lymphocytes and B7 molecules expression on the primary breast cancer cells in patients with breast cancer. MethodsFlow cytometry was used to analyze CD28 and B7 molecules expressed on T lymphocytes and primary breast cancer cells respectively. Results CD28 positive T lymphocytes in breast cancer patients was lower than that in control group (t=2.879,P

ve To establish a method for determination of arsenic in drinking water. Methods The contents of arsenic in water samples were determined directly by graphite furnace atomic absorption spectrophotome-try with masking agent of palladium. The working conditions were defined as wave length of 193.7 nm, slit width of 1.3 nm, current of hollow cathode lamp of 12.0 mA; drying 40 sec at 80℃~140℃ ; ashing 20 sec at 1100℃~1200℃; atomizing 5 sec at 2 300℃; purifying 4 sec at 2 600℃ . Results The linear relationship was 10~200?g/ L ar-senic. The average recovery rates of water samples at concentrations of 50 ?g/ L and 150?g/ L arsenic after being added arsenic standard solutions were 100.7% and 103.6% respectively, and sensitivity reached 10?g/ L. The cor-relation coefficient were 0.9954~0.999 7 obtained from 6 randomly repeated determination of standard curve. The metal ions coexisting in determined water samples didn't interfere the determination of arsenic under the application of common uncoated graphite tube. The 0.2% aluminum didn't disturb the determination of arsenic also. Conclusion This method could be applied in the determination of arsenic in water, especially could be applied in the direct deter-mination of arsenic in more clear water samples.

Objective To observe the effect of case-based learning (CBL) applied in ward-round on residents training. Methods A total of 33 residents trained with routine ward-round and CBL ward-round in the neurological department were investigated with questionnaire. Results 30 effective answers were recalled. More of them reported that CBL ward-round was very significant in improving clinical thinking, mastering the specialized knowledge, developing autonomous learning and improving the communication with patients and their family members (P<0.05). Conclusion CBL ward-round is effective on improving the comprehensive professional abilities of residents.

OBJECTIVETo perform a transcriptomics study in differential genes after Xianglian External Lotion (XEL) induced the recovery of drug-resistant Candida albicans strains sensitive to Fluconazole.

METHODSBroth microdilution antifungal susceptibility test was used to detect minimal inhibitory concentration (MIC) of drug-resistant Candida albicans strains induced by XEL. Transcriptome sequencing (RNA-Seq) was used to determine and compare the transcription of primary drug-resistant Candida aIbicans strains and sensitive strains induced by XEL. High expressed genes and signaling pathways strains were analyzed by gene ontology (GO) method.

RESULTSXEL could induce drug-resistant strains of the 6th generations to recover sensitivity. Transcriptome sequencing showed that, as compared with primary drug-resistant strains, there were 165 genes with up-regulated RPKM index and 144 genes with down-regulated RPKM index after XEL induction. GO analyses found that all genes were mainly classified as GO:0015903 (fluconazole transport).

CONCLUSIONSXEL could induce the recovery of drug-resistant Candida albicans strains sensitive to Fluconazole. By analyzing transcriptomes, authors speculated that XEL could recover strain sensitivity to fluconazole by opening fluconazole transport pathway.

Antifungal Agents ; pharmacology ; therapeutic use ; Candida ; Candida albicans ; drug effects ; genetics ; Candidiasis ; drug therapy ; Drug Resistance, Fungal ; drug effects ; genetics ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Fluconazole ; pharmacology ; Microbial Sensitivity Tests

As an important indicator of total hip arthroplasty (THA) the rate and degree of offset reconstruction play an important role in improving the prognosis and life quality of patients. The reconstruction of femoral offset is closely related to reserved length of calcar femorale, the head and neck length of prosthesis, angle degree of neck shaft and whether lower limb is isometric. Reconstruction strategy includes making a meticulous and standard measurement before the surgery, predicting the reserved length of calcar femorale, selecting a prosthesis with approximate anatomical neck-shaft angle and reconstructing offset by adjusting the head and neck length of the prosthetic during the operation. The aim of this article was to introduce the research progress and influence of offset on hip function, prosthetic wear and postoperative complications such as pain, limp and unequal leg length, and to discuss the reconstruction strategy.
Arthroplasty, Replacement, Hip ; adverse effects ; methods ; Femur ; surgery ; Humans ; Reconstructive Surgical Procedures ; adverse effects ; methods

Objective To investigate the conceptual feasibility and biomechanical advantages of our self-designed screw hole filler for a lateral bamboo-raft-type titanium locking plate in a digital model of proximal tibial fracture using linear statics finite element analysis.Methods A nut-type screw hole filler was designed.Four different idealized finite element models of proximal tibial fracture fixed by a lateral bamboo-raft-type anatomic locking plate were established on the basis of three-dimensional CT reconstruction and digital orthopedic techniques.The first one was assembled with the filler and stabilized fibula,the second with stabilized fibula but without the filler,the third with the filler and unstabilized fibula,and the fourth with unstabilized fibula but without the filler.Linear statics finite element analyses were conducted for the 4 models under the same 26 groups of loads.The 4 models were compared in terms of maximum relative displacement on fracture surface,average relative displacement on fracture surface,maximum Von Mises stress of internal fixation,maximum contact stress on fracture surface,maximum contact stress between plate and tibial surface,maximum contact pressure on fracture surface,and maximum contact pressure between plate and tibial surface.Outcomes of the analyses for the 4 models were harvested and treated with Wilcoxon Signed Ranks Test.Results There were significant differences between using filler and not using filler in maximum relative displacement on fracture surface,average relative displacement on fracture surface,maximum Von Mises stress of internal fixation and maximum contact pressure between plate and tibial surface (P ＜ 0.05),but no significant difference was found in maximum contact pressure on fracture surface (P ＞ 0.05).The model assembled with the filler and unstabilized fibula led to the greatest improvement in maximum Von Mises stress of internal fixation (P ＜ 0.05).Conclusions Since use of nut-type screw hole filler may increase the contact pressure between plate and bone surface,reduce the maximum Von Mises stress of internal fixation,the maximum displacement of the whole model,and the relative and average displacements on fracture surface,but has insignificant effect on the contact pressure on fracture surface,it improves the biomechanical performance of internal fixation.The filler deserves further study.