Objective To realize the bacterial contamination on uniforms of health care workers(HCWs)in a gen-eral hospital,and put forward the corresponding management measures.Methods In May-October 2012,a total of 360 specimens of 120 uniforms of HCWs in departments of respiratory internal medicine,general surgery,gynecolo-gy,and pediatrics were taken on the first,third and seventh day of wearing,bacterial counts on uniforms were mo-nitored,compared and analyzed.Results Bacterial counts of uniforms at different wearing time were statistically differ-ent.The longer time of uniforms were worn,the more bacteria could be detected.Bacterial contamination of nurses’uni-forms was more serious than doctors ([0.65±3.38]CFU/cm2 vs [7.68±2.99]CFU/cm2 ),contamination of uniforms of HCWs in surgical departments was more serious than non-surgical departments([10.43±4.12 ]CFU/cm2 vs [8.60± 3.01]CFU/cm2 )(U=5.06,2.78,respectively,both P<0.01),over standard rate of different sites of HCWs’uniforms were significantly different(χ2 =33.12,P<0.01));over standard rates of bacteria on the cuffs,abdomen and chest sites was 73.33%,58.33% and 36.67% respectively.Conclusion The management of cleaning system of HCWs’uniforms needs to be strengthened,the change cycle of uniforms is suggested twice a week,and the frequency needs to be increased in high contamination departments.

Objective To explore the drug fast mechanism on carbapenems of Acinetobacter baumannii,it is important for clinicians to choose appropriate antibiotics.Methods The influence of active efflux inhibitors carbonyl cyanide m -chlorophenylhydrazone (CCCP)on minimal inhibitory concentration (MIC)of Acinetobacter baumannii to imipenem(IMP)was measured by agar dilution method.Results At the concentration of 10mg/L,CCCP had obvi-ously effects on carbapenems MICs,the rate of resistance of imipenem decreased from 64.39% to 34.38%.In vitro CCCP enhanced the activities of imipenem.Efflux mechanism existed not only in IMP resistant strains,but also in IMP sensitive strains.But the affection of CCCP against IMP resistant strains was much stronger than that of IMP suscepti-ble strains.Conclusion Efflux pump may be one of the main mechanisms of Acinetobacter baumannii resistance to IMP,while efflux pump inhibitors may partly reduce the resistance.

Objective To explore the clinical significance of changes of serum.IL-6,IL-18 and C-reactive protein(CRP) in patients with hypoxic ischemic encephalopathy (HIE).Methods Serum IL-6,IL-18 and CRP levels were measured by ELISA and immunity turbidimetry respectively in 100 patients with HIE and 100 health control.Results The HIE group serum IL-6,IL-18 and CRP levels were higher than the healthy control group,and Serum levels of IL-6,IL-18 and CRP increased when the clinical manifestation gradually increased.The CRP were positive correlation with serum levels of IL-6 and IL-18(r=0.663,0.725,all P<0.01).and the serum level of IL-6is positive correlation with IL-18(r=0.783,all P<0.01).Conclusion The serum IL-6,IL-18 and CRP may play an important role in the development process of HIE.Which were correlate to the severity of the disease and clinical change,measurements of their serum concentrations may help to predict these pathogenetic condition and prognosis criterion of the disease.

Cells, Cultured ; Endothelial Cells ; drug effects ; metabolism ; Endothelium, Vascular ; cytology ; metabolism ; Gene Expression Profiling ; Humans ; Ouabain ; pharmacology ; Umbilical Veins ; cytology

Objective To research the lymphatic tropism and lymph cell apoptosis of cisplatin-nano carbon suspension in rats with the aim of proposing a new way for chemotherapy.Methods A total of 72 Wistar rats were randomly divided into two groups.For the experimental group,cisplatin-nano carbon suspension 0.3 ml (4 mg/ml) was injected subcutaneously into Wistar rats' plantar.For the control group,cisplatin 0.3 ml (4 mg/ml) was injected intravenously.Cisplatin concentration in the inguinal lymphatic tissue and plasma was determined by high performance liquid chromatography (HPLC) at 1,2,3,4,5 and 6 hours after drug administration.The apoptosis of lymph cell was detected by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling assay (TUNEL).Targeting ability were evaluated and compared by targeting index (TI),selecting index (SI) and relative extraction efficiency (RE).SPSS 17.0 statistical software was used to analyze the differentiation of the cisplatin concentration and apoptosis index (AI) among various groups.DAS software was used to evaluate the lymphatic tropism.Results The cisplatin concentration of lymphatic tissue in experimental group were respectively (1.03±0.32),(3.00±0.91),(2.20±0.73),(1.56±0.38),(1.30±0.74) and (0.78±0.34) μg/g after administration 1,2,3,4,5 and 6 hours,while in control group were (0.49±0.21),(1.02±0.70),(0.59±0.50),(0.56±0.21),(0.47±O.18) and (0.36±0.13) μg/g,in which there were significant difference at every times (all P＜0.05) except at 1 hour (P=0.173).The cisplatin concentration in plasma were significant higher in the experimental group than those in the control group at various times (all P＜0.05),the former were respectively (0.57±0.28),(1.22±0.45),(0.61 ±0.18),(0.51 ±0.13),(0.45 ±0.13) and (0.40±0.07) μg/ml,while the latter were respectively (3.12± 0.33),(4.09± 0.48),(2.56 ± 0.38),(2.05 ± 0.13),(1.81 ± 0.28) and (1.44± 0.40) μg/ml.Values of TI were respectively 2.12,2.93,3.73,2.78,2.76 and 2.19 and SI were 1.80,2.45,3.63,3.07,2.86 and 1.93.Value of RE was 2.86.The AI in experimental group were respectively (16.5±5.2)％,(30.2±2.8)％,(51.7±4.3)％,(69.8±3.2)％,(80.1 ±4.3)％ and (89.7±8.5)％,while in control group were respectively(1.3±0.8)％,(2.4±1.7)％,(3.2±1.1)％,(3.9±2.6)％,(5.1±2.1)％ and (6.3±2.3)％,in which there were significant difference at every points (all P＜0.05).Conclusions The nano carbon has the character of lymphatic tropism,and could send cisplatin to lymphatic tissue to achieve a higher concentration.The trait may break a new way for chemotherapy targeting lymph metastasis.

Objective To study the morphologic and pathological characteristics of cat scratch disease(CSD).Methods Eight cases with clinical data and tissue blocks were collected in Guangxi Zhuang Autonomous Region and Hainan Province.The tissues were successively stained by hematox- ylin and eosin,Warthin-Starry(W-S),acid fast and periodic acid-schiff(PAS)methods to study the histopathological changes and pathogens.Results W-S positive Bartonella henselae was the major pathogen of CSD and there was no acid-fast or PAS positive pathogen could be found in the tissues. There were three forms of histological representation as follows:plasmocytoid monocytes(PMO)and monocytoid B-cells(MBC)hyperplasia plus neutrophils immersion in lymphatic sinus(2 cases); MBC rich granuloma and micro-abscess formation(3 cases); starlit abscess with little or no bacteria in the granuloma(3 cases).Conclusions Bartonella henselae mainly transmits through cats.Contact histo- ry with cats and lymphadenectasis suggest the possibility of CSD.The diagnosis can be confirmed by the presence of W-S staining positive bacteria,MBC rich granuloma or micro-abscess and neutrophil reactions in histopathological exam.

Spermicidal effect of Jieze No. 1 (JZ1) in combination with nonoxynol-9 (N-9) was examined in vitro. The minimum spermicidal concentration of JZ1 decoction, N-9 and their mixture solution in 20 s and 3 min were examined by improved spermicidal test of Sander-cramer in vitro. The percentages of progressively moving spermatozoa, moving spermatozoa and viable spermatozoa were also observed 20 s, 3 min and 30 min after the addition of the liquid medicine. Our results showed that sperms did not recover their activities in a revival test when the minimum spermicidal concentration of either JZ1 decoction, or N-9, or the mixed solution of the two agents, was used. N-9 (JZ1 in the mixed group) showed significant differences in the percentages of progressively moving spermatozoa, moving spermatozoa, and visible spermatozoa in 20 s, 3 min, and 30 min, when compared with N-9 alone (P < 0.01). We are led to conclude that JZ1 decoction can improve N-9 spermicidal action in vitro, and when used in combination with N-9, it has synergic effect.
Drugs, Chinese Herbal/*pharmacology ; Nonoxynol/*pharmacology ; Semen/drug effects ; Spermatocidal Agents/*pharmacology ; Spermatozoa/*drug effects

Objective:To assess the vision-related quality of life(VRQoL) of the patients with simple vitreous hemorrhage after vitrectomy and analyze its influencing factors.Methods:A scale of life quality for patients with visual impairment(AQoL-DVI) were administered to 96 patients before and 6 months following vitrectomy.Scale scores were compared with vison preoperatively and postoperatively.Results:The lowest scores were at the item of "social life" and "spiritual and psychological status" before the surgery.The scores of the questionnaires increased significantly after vitrectomy.The change of visual acuity after surgery was a chief independent factor of the changes of questionnaire scores due to vitrectomy.Conclusion:The quality of life of patients with simple vitreous hemorrhage decreases sharply.VRQoL improves obviously after vitrectomy.

AIM: To investigate the mechanism of AngRem104-mediated regulation of fibronectin gene in human mesangial cells. METHODS: A series of deleted FN promoter sequences was constructed, which fuse to a luciferase reporter gene, and then the potential active regions that respond to AngRem104 in the upstream regulatory sequence of human FN gene was screened by detecting the luciferase activity of promoter-reporter gene. RESULTS: The detection of relative luciferase activity revealed that there was no significant differences when the HMC were transfected with FN122-reporter gene together with sense AngRem104 construct, but the luciferase activity significantly increased when transfection of FN507-reporter gene construct together with sense AngRem104 construct. However, no increase in luciferase activity was observed when transfection of FN1280-reporter gene construct together with sense AngRem104 construct. The potential regulatory region responds to AngRem104 is in the upstream sequence (-122 to -507) of human FN gene. CONCLUSION: Our preliminary study provided the evidence that AngRem104 may mediate the transcription of the FN gene via the activation of its promoter.

Objective:To investigate the effect of all-trans retinoic acid (ATRA) on the differentiation of marrow stromal stem cells(MSCs)into neurons. Methods: Rat MSCs were expanded as undifferentiated cells in culture for 5-7 passages and pretreated with ATRA for 24 h, then induced by modified neuronal medium (MNM) for 18 h. The control cells were directly cultured in MNM without ATRA pretreatment. Immunocytochemistry was used to detect the expression of nestin, neuron-specific enolase (NSE), neuron-specific nuclear protein (NeuN) and microtuble-associated protein (MAP-2) in the experimental groups and the control cells. The change of cell proliferative cycles and the expression of retinoic acid receptor beta (RAR?) were detected before and after ATRA treatment. Results: 1. The expression of nestin, NSE, NeuN and MAP-2 was much higher than that of the control group. 2. No changes were found in the cell proliferative cycles before and after ATRA treatment. 3. No expression of RAR? was found in MSCs; but positive expression of RAR? was detected afer 24 h of ATRA treatment and it was (20.3?4.2)%. Conclusion: ATRA can activate gene transcription via nuclear receptor RAR? and promote MSCs transdifferentiation into neurons.