Objective To observe the clinical efficacy of aconite-cake-partitioned moxibustion plus medication in treating coronary heart disease (CHD) with stable angina pectoris.Method Ninety patients with CHD stable angina pectoris were randomized into a treatment group of 46 cases and a control group of 44 cases. The control group was intervened by the secondary preventive treatment for CHD, while the treatment group was intervened by aconite-cake-partitioned moxibustion at Neiguan (PC 6), Guanyuan (CV 4) and Danzhong (CV 17) in addition to the treatment given to the control group. The two groups were treated once a day, for a total of 10 sessions. The reduction-termination rate of Nitroglycerin and clinical efficacies were compared between the two groups.Result The total effective rate was 89.1% in the treatment group versus 72.7% in the control group, and the between-group difference was statistically significant (P<0.05). The reduction-termination rate of Nitroglycerin was 84.8% in the treatment group versus 63.6% in the control group, and the between-group difference was statistically significant (P<0.05).Conclusion Aconite-cake-partitioned moxibustion is an effective method in treating CHD with stable angina pectoris.

Objective Toestablishanultra-performanceliquidchromatography-quadrupole-electrostaticfieldorbitraptandem mass spectrometry(UPLC-Q-Orbitrap-MS/MS) analysis technology for the identification of different variants of inactivated SARS-CoV-2 vaccines(Vero cells), so as to provide a new evaluation direction for vaccine supervision.Methods Peptide samples were obtained from inactivated SARS-CoV-2 vaccines after reductive alkylation, tryptic digestion, and desalting of SDB-RPS desalting column, and then were eluted by CDS C18 column(0. 075 mm × 250 mm × 1. 9 μm) in gradient program, with 0. 1% formic acid and 3% dimethyl sulfoxide as mobile phase A, 0. 1% formic acid, 3% dimethyl sulfoxide and 80% acetonitrile as mobile phase B. The column temperature was 50 ℃, the flow rate was 300 nL/min, and the injection volume was 5 μL. Then UPLC-Q-Orbitrap-MS/MS analysis technology was established, the mass spectrometry detection was scanned by data-dependent acquisition(DDA) mode, and the sample peptide information was retrieved and analyzed by MaxQuant(V 2.4.2.0) software.Results The established method was used to analyze and determine the inactivated SARSCoV-2 vaccines(Vero cells) of different variants, and the conserved peptides and characteristic peptide sequences of S protein and N protein of different variants of SARS-CoV-2 were screened and extracted, and the peptide mass-to-charge ratio(m/z) was combined to obtain the specific protein database of different variants. By comparing the peptide information detected by the mass spectrometry of the sample, it is possible to identify different variants of inactivated SARS-CoV-2vaccines(Vero cells).Conclusion The UPLC-Q-Orbitrap-MS/MS analysis technology established in this study has the advantages of high sensitivity, rapidity and strong specificity, which can be effectively used for the identification and screening of strains of inactivated SARS-CoV-2 vaccines(Vero cells), can provide a technical reference for the identification of SARS-CoV-2 species in other viral vaccines or biological samples, and provide a new perspective for the quality and safety supervision of vaccines.

Drugs, Chinese Herbal ; therapeutic use ; Humans ; Medicine, Chinese Traditional ; Psoriasis ; therapy

Arthritis, Rheumatoid ; diagnosis ; drug therapy ; Diagnosis, Differential ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Humans ; Male ; Medicine, Chinese Traditional ; methods ; Phytotherapy ; methods

Cutaneous wound healing is a complicated multiatep process with numerous mediators that act in a network of activation and inhibition processes. Nonhealing chronic wounds decrease the quality of Life. Recombinant growth factors, currently used in clinic, fail to provide a sustained repair function at the wound due to their inadequate biological availability and transient half-time. Recent attention has focused on gene therapy, which might become a significant treatment modality for those wound healing pathologies refractory to other wound management approaches. This review discusses the potentials and limitations of current genetherapy for the treatment of wounds, current ongoing clinical trials, and possible future directions in this exciting field.

BACKGROUND:It can be confirmed by the clinical trial that chronic plantar fasciitis can be treated by extracorporeal shock wave.There are some different explains for the mechanism of action.OBJECTIVE:To introduce the mechanism of action and clinical application of extracorporeal shock wave in the treatment of chronic plantar fasciitis.RETRIEVAL STRATEGY:A computer-based search was conducted in PUBMED,HighWire and Springer databases for articles published before August 2007 with the key words of "chronic plantar fasciitis,extracorporeal shock wave application" in English by the first and second authors.Inclusive criteria:articles were selected firstly based on the mechanism of action and clinical application of extracorporeal shock wave for chronic plantar fasciitis.Exclusive criteria:articles out-of-date and repeated were removed.LITERATURE EVALUATION:Sixty-five relevant articles were collected,and twenty-five met the inclusive criteria.Of them,4 articles were review,and others were about the mechanism of action and clinical application of extracorporeal shock wave for chronic plantar fasciitis.DATA SYNTHESIS:①Someone believed that high energy shock wave selectively damaged unmyelinated sensory nerve fibers.Low energy shock wave could inhibit the conduction of sensory nerve ending by releasing calcitonin-gene-related peptide and producing neurogenic inflammation.It also could play a long-term analgesic effect by making the changes in regional flow of the brain,regulating pain memory and reducing local stimulatory response.②There are many reports on short-term and long-term outcomes,different energy levels,local anesthetic drug,the depth of plantar fasciitis and therapeutic place.CONCLUSION:It is effective to treat chronic plantar fasciitis by extracorporeal shock wave.Different approaches can bring us different therapeutic outcomes.

The ultrastructural changes of atrial myoendocrine cells (AMC) in various volume loading for heart, the type and the distribution of nerve endings and capillaries in the vicinity of AMC were observed with electron microscopy. The rats deprived or undeprived water received an injection of isotonic saline via the femoral vein (20 ml/mg), then the right auricle was removed and fixed with usual method for EM and partly with TAGO method. The process of atrial specific granules (ASG) secretion, i. e., the limiting membrane of ASG fused with the sarcolemma and opened at this place, and the ASG content was released through this opening into extracellular space, were observed. There were various types of nerve endings nearby AMC. The capillaries were continuous endothelium in type, and show close relation to AMC. The results of this study suggest that AMC secrete ANF by exocytosis mainly, and provide some morphologic data for the physiological and biochemical studies of ANF move and of reaction on each other between the ANF and nerves.

Objective:To develop a method for the rapid identification and determination of gatifloxacin injections by Raman spec-troscopy. Methods:Raman spectroscopy was used as the method of identifying and determining gatifloxacin injections. The determina-tion conditions were as follows:a micro Raman spectroscopy, excitation wavelength:785nm, objective lens:50X, laser power:3mW, signal acquisition time:60s. Results:The result showed that the Raman spectroscopy could identify and determine gatifloxacin injec-tions, the content of gatifloxacin in the injections was respectively 99. 49%,97. 56% and 100. 2%(Specification:10ml∶0. 2g), and 97. 75%, 94. 58% and 96. 25%(Specification:2ml∶0. 1g), and there was no significant difference between the above determination results and the results from the HPLC method by t-test. Conclusion:For the fast and nondestructive properties, the presented method can be developed as an analysis method for gatifloxacin injections.

Objective To compare the clinical features of myocardial infarction (MI) in young adults with and without diabetes. Methods A total of 94 young adults with MI were enrolled in this study and divided into two groups according to whether they had diabetes:diabetic group (DM) and Non diabetic group (NDM).The clinical features,angiographic results,risk factors and prognosis were compared between the two groups. Results The incidence of atypical chest pain (25.0% vs 7.6%),non-ST segment elevation myocardial infarction (50.0% vs 9.1%),ventricular wall motion abnormalities (78.6% vs 42.4%),double vessels (35.7% vs 21.2%),triple vessels (39.3% vs 3.1%) and the incidence of mortality(10.7% vs 1.5%)were all significantly higher in DM group than in NDM group (P<0.05 or P<0.01).The number of affected vessels and Gensini scores were (2.110±0.875) and (52.61±10.47) in DM group,and(1.140±0.677),(34.02±10.24) in NDM group.The between group difference was statistically significant (P<0.05).Multiple linear regression showed that HbA1c and smoking were the independent risk factors for Gensini score. Conclusion The clinical features of young adult patients with MI and diabetes are more complex,their coronary stenosis are more serious,and the prognosis is poor.Smoking and HbA1c are positively associated with Gensini scores.

Objective: To establish a method for HPLC determination of schisanthein, deoxyschizandrin and anwuligan in Wuzhi pellets simultaneously and control the quality of Wuzhi pellets.Methods: Schisantherin, deoxyschizandrin and anwuligan were determined by HPLC with a chromatographic column of Welch Ultimate XB-C 18 (150 mm× 4.6 mm , 3 μm), the mobile phase was tetramathylene oxide-water with gradient elution.The detection wavelength was 228 nm.The column temperature was 35℃.Results: The linear range of schisantherin was 0.040-0.805 μg (r=0.999 9),and the average recovery was 97.0% with RSD of 1.67%.The linear range of deoxychizandrin was 0.092-1.846μg (r=0.999 9),and the average recovery was 102.5% with RSD of 1.49%.The linear range of anwuligan was 0.020-0.998 μg (r=0.999 9),and the average recovery was 95.0% with RSD of 1.78% (n =6).Conclusion: The method is simple, accurate and reproducible.It can improve the quality standard of Wuzhi pellets.