Objective To study the effects of electromyography-triggered neuromuscular stimulation on upper extremity functional recovery of acute stroke patients. Methods 60 stroke patients who lost wrist extension even in the electromyography (EMG) within 4 weeks were randomly assigned to experimental group and control group. The experimental group received electromyography-triggered neuromuscular stimulation, and the control group received neuromuscular stimulation. They were assessed with EMG, Fugl-Meyer assessment (FMA), Barthel index (BI) before and 4 weeks after treatment. Results The EMG, scores of FMA and BI improved in all the patients after treatment (P=0.000), and improved more in the experimental group (P<0.05). Conclusion Electromyography-triggered neuromuscular stimulation can improve the motor function of hemiplegia, even in the patients without change of EMG.

0.05).The effective rates of the two treatment schemes were 90%,87%.The cost effectiveness ratio were 108.61 and 76.35.Conclusion Homemade octreotide is better in the treatment of severe acute pancreatitis.

58 embryos at 1, 2, 3 and 4 cell and morula stage were recovered from the oviduct and the uterine horn of the Ha-bai, Du-ha and Du-chang-ha pigs, 24 hours, 2 or 3 days of pregnancy, and were observed with SEM and TEM. The zona pellucida of 1 cell embryo was removed by treatment with 2.5％ pronase for visualization of the cell surface structure.The findings are as follows:1. The surface of the 1 cell stage embryo was densely populated with microvilli and sperm can be found on the surface with its head and tail surrounded by the microvilli and its plasma membrane of the equatorial segment of acrosome intact.2. Many spermatozoa were observed both on the surface and inside the zona pellucida of the 2 celled embryo.3. By the 4 cell stage, some of the spermatozoa had penetrated through the zona and got into the perivitelline space. 4. There were still some spermatozoa on the zona surface of the morula.5. The TEM observation revealed that the heads of supernumerary sperm were located within the cytoplasm of the blastomeres of the embryos from 1 to 4 cell stages and still not decondensed. Some of these sperm heads were actually found within the phagocytic vacuoles of the 4 celled embryos. The ultrastructure of these polyspermic embryos was normal.These results showed that polyspermy in the pig did not intefere with the cleavage of the embryo and the embryo possessed the ability to dispose the excessive sperms.

On 10 days of pregnancy the blastocysts of 3 mm and 9 mm in diameters wereflushed out from the uterine horns of the Du-Chang-Ha and Dong-Bei-Min pigs.The blastocysts were divided into four parts,that is the embryonic disc,the animalpole,the lateral part and the vegetal pole.The outer and inner surface of the fourparts were observed with SEM and the findings are as follows:1,The 3 mm blastocystsThe outer surface of the embryonic disc was still covered with trophoblasticcells.Three kinds of cells can be discerned:the cell with round prominences andlong microvilli(MV);the cell with thin and long MV,the cell with smooth sur-face.The trophoblast cells in other areas of the blastocyst were similar but slightlyflat.The inner surface of the blactocyst consists of endoderm cells which weredifferent in the four parts.From the embryonic disc to the vegetal pole,the surfacestructure of the endoderm cells changed regularly from small to large from dome-shaped to flat and the distance between nuclei increased gradually.The MVs on thecell surface:became less.These findings suggested that endoderm cells weremoving from the center of the embryonic disc to the vegetal pole.There weremany holes on the marginal area of the cells.The edge of the cell on the innersurface of the vegetal pole forms a network.Many round granules can be seen inthe holes of the network.Their function may be for the transport of nutrients.2,The 9 mm blastocystsThe outer surface of the embryonic disc was not covered with trophobla-stic cells but covered with ectoderm cells.The ectoderm cells in the primitivestreak had long MV on their surface but short ones on other areas of the disc.The inner surface of the embryonic disc was endoderm cells.The MVs on theendoderm cells was much more numerous than the MVs on the ectoderm cells.Thedifferentiation of the cells in the pig blastocyst was also discussed.

Objective To investigate the inhibitory effect of ginkgolide B (BN52021) on severe acute pancreatitis (SAP) via detecting the antagonistic effect of BN52021 on platelet-activating factor (PAF). Methods One hundred and eighty Wistar rats were randomly divided into control group (n = 60), SAP group (n = 60) and BN52021 group (n =60) according to the random number table. The 3 groups were divided into 6 subgroups at different time points after operation (1 h, 2 h, 3 h, 6 h, 12 h, and 24 h). The changes of serum amylase in each group were monitored. The expression of platelet-activating factor receptor (PAFR) mRNA and protein was detected by RT-PCR and Western blot, and the pathological changes of pancreatic tissues were observed. All the data were analyzed by one-way ANOVA. Results Serum amylase level and pathological results showed that it was successful in preparing SAP model. The serum amylase levels at postoperative hour 3, 6 and 24 were (4185 ±148) U/L, (3785 ± 124) U/L and (1360 ± 161) U/L in BN52021 group, which were significantly lower than those in SAP group [(4799 ± 107) U/L, (4920 ± 140) U/L, (2283 ± 127) U/L)]. The pathological scores at postoperative hour 3, 6, 12 were 5.95±0. 19, 5.55±0.36, 6.72±0. 30 in BN52021 group, which were significantly lower than those in SAP group (8.85 ± 0.39, 9.15 ± 0.55, 10.10 ±0. 65). The mRNA and protein expression of PAFR were gradually increased at the early stage (0.49 ± 0.09-0.71 ± 0.14 vs 0. 43 ～ O. 06-1.69 ± 0.06), and reached peak at postoperative hour 3. The expression levels of PAFR mRNA and protein in BN52021 group and SAP group at postoperative hour 3 had statistical difference among the 3 groups (F = 4.58, 6.24, P < 0.05). Conclusions The expression of PAFR mRNA and protein in the pancreatic tissue of SAP rats is dynamically changing. PAFR plays an important role in the occurrence and progression of SAP. BN52021 can reduce the expression of serum amylase and improve the pancreatic pathological changes, but it has no effect on the expression of PAFR in pancreatic tissue.

Purpose To investigate the clinicopathological changes, immuniohistochemistry and molecular genetics phenotypic charac-teristics of the alveolar soft part sarcoma ( ASPS) . Methods 16 cases of ASPS were studied with clinicopathological, cytochemistry technique and immunohistochemical staining, two cases of ASPS were studied by FISH. Results There were 6 males and 10 females with the age 8~58 years (median age 31. 7 years). The tumors were located at limbs, shoulder and back, tongue, vocal cords, lung, cervix, and ureter. The clinical manifestations of the patients was a slowly growing mass. Histopathologically the tumor showed typical organ-like or acinar-like structure with sinus-like blood vessels and the fibrous septa formation. Sometime the clear or abundant eosino-philic granular cytoplasm of the tumors were obvious. The tumor cells had a crystalline substance formation by PAS staining. The tumor cells were positive for TFE3 and Cathepsin K by immunohistochemical staining. The ASPL-TFE3 gene fusion detection of tumor cells were present. Conclusion ASPS often located on the limbs of young patients. It may misdiagnosed as malignant epithelial tumors, primary or metastatic adenocarcinoma and paraganglioma when the tumor locate on a rare anatomical parts or an organs, such as tongue, vocal cords, cervix, ureter, etc. It is valueable that the typical alveolar-like structure of the tumor and the expression for TFE3 and Cathepsin K for the pathological diagnosis of ASPS. It is an important indicator that the ASPL-TFE3 gene fusion detection by FISH for the tumor.

Purpose　To investigate the diagnostic valuation of Gd-DTPA enhancement MRA in intracranial occuping and vescular diseases. Methods　Gd-DTPA MRA（63 cases）, MRA without Gd-DTPA（16 cases）as a control group was performed with Elscint Prestige 2.0T MR,MRA. 56 cases were proved by operation or pathology, 7 cases were proved by clinical observation. Results　The shape of aneurysms, the feeding arteries, the draining veins of AVM and diploe angioma, the feeding arteries of occuping were clearly showed by Gd-DTPA enhancement MRA and were not showed by MRA without Gd-DTPA.There wasn't much difference between cavernous angioma with Gd-DTPA enhancement MRA and MRA without Gd-DTPA in the shape and blood supply of cavernous angioma. Conclusion　Gd-DTPA enhancement MRA is useful and worthy for showing blood circulation of intracranial occuping and vascular diseases.

Objective To study the effect of Fas gene transfection on bladder cancer cells. Methods Fas cDNA were transduced into bladder cancer cells EJ by DOTAP liposomal transfection regeant. Northern blot,in situ hybrization and flow cytometric evaluation were used to confirm the Fas mRNA and protein expression. The apoptosis and proliferation of EJ cells pre and posttransfection induced by cisplatin were analysed by flow cytometry、DNA ladder and MTT methods. Results Transfection of Fas gene can significantly upregulate the expression of Fas in human bladder cancer EJ cells. Apoptosis and decrease of proliferation were easily induced by cisplatin in the transfected EJ cells. Conclusions Fas system might involve in the development and progression of urogenital malignant tumors. Transfection of Fas gene by lipofectin can significantly upregulate the Fas expression in target cell. The synergistic cytotoxic effect obtainted in EJ cells suggested that combined use of Fas gene transfection and cisplatin may help in the treatment of cisplatin resistant bladder cancer.

12E7 Antigen ; Adolescent ; Alkaline Phosphatase ; metabolism ; Antigens, CD ; metabolism ; Cell Adhesion Molecules ; metabolism ; Diagnosis, Differential ; Female ; Follow-Up Studies ; Gonadoblastoma ; pathology ; Humans ; Immunohistochemistry ; Isoenzymes ; metabolism ; Neoplasms, Germ Cell and Embryonal ; metabolism ; pathology ; surgery ; Ovarian Neoplasms ; metabolism ; pathology ; surgery ; Proliferating Cell Nuclear Antigen ; metabolism ; Sex Cord-Gonadal Stromal Tumors ; metabolism ; pathology ; surgery

Objective To explore the clinical significance of urine transferrin testing in the diagnosis of early pregnancy-induced hypertension syndrome (PIH).Methods Sixty-eight PIH patients (PIH group) who received treatment from January 2012 to October 2013 were enrolled in this study.Mild PIH was 21 patients,midrange PIH was 24 patients and severe PIH was 23 patients.Meanwhile,68 cases of healthy person were selected as control group.The level of urine transferrin,urea and creatinine were compared between two groups.Results The level of urine transferrin,urea and creatinine in PIH group were significantly higher than those in control group[(3.01 ± 1.82) mg/L vs.(1.29 ± 0.88) mg/L,(13.12 ± 5.51) mmol/L vs.(6.23 ± 3.68) mmol/L,(135.5 ± 62.3) μ mol/L vs.(85.1 ± 38.2) μ mol/L] (P ＜ 0.05).The sensitivity of urine transferrin,urea and creatinine in mild PIH was 76.19％,61.90％,57.14％.In midrange PIH was 75.00％,79.17％,75.00％.In severe PIH was 78.26％,86.96％,73.91％.The specificity of urine transferrin,urea and creatinine was 80.65％,56.45％,58.06％.The sensitivity of different degree of PIH was exceeded 75.00％.The sensitivity of urine transferrin in mild PIH was significantly higher than urea and creatinine (P ＜ 0.05).But the sensitivity of urine transferrin in midrange PIH and severe PIH had no significant difference (P ＞ 0.05).The specificity of urine transferrin was significantly higher than urea and creatinine (P ＜ 0.05).Conclusions Urine transferrin is a good index in diagnosis of early PIH,which can reflect the early injury degree of the renal tubular and glomerular.It has active and important significance for the clinical diagnosis and treatment of PIH.