Objective The study is to investigate the effect of tanshinone on lung reperfusion injury. Methods 24 New-Zealand rabbits were randomly divided into two groups. In one group, the lungs were perfused and preserved with LPD solution, the other with LPD+ tanshinone solution. The isolated lungs were preserved for 18 hours and then reperfused with rewarming whole blood. After reperfusing 30 minutes the lung samples were examined by pathology, wet/dry weight and measure of NO, SOD and MDA. Results In two groups, the reperfusion injury lungs were fresh, but had partial congestion, edema and ecchymoma under naked eye, under light microscopy the structures of the pulmonary vesiculars, branchia and capillaries were complete, but the epithelial cells of the vesicles and capillaries in the LPD group were more swelling and hypertrophy than that in LPD+ tanshinone group. The wet/dry weight ratio was significantly lower in LPD+ tanshinone group than in the LPD group. NO [(2.16±2.15)μmol/g] and SOD [(37.03±18.01NU/mg] were higher in the LPD+ tanshinone group than in the LPD group [(0.68±0.33) μmol/g, (17.63±11.04)NU/mg] (all P<0.05), MDA was lower in the LPD+ tanshinone group [(0.47±0.20)μmol/mg] than in the LPD group [(0.86±0.44)μmol/mg] (P<0.05). Conclusions Tanshinone can dean oxygen free radical and is of stronger antioxidation, results in alleriating of lung reperfusing injury. Tanshinone has positive effect on preservative quality of the reperfusion injury lung.

Adolescent ; Child ; Child, Preschool ; DNA Mutational Analysis ; Diagnosis, Differential ; Humans ; Infant ; Infant, Newborn ; Liver Diseases ; diagnosis ; genetics ; pathology ; Lung Diseases ; diagnosis ; genetics ; pathology ; Magnetic Resonance Imaging ; Mutation ; Polycystic Kidney, Autosomal Dominant ; diagnosis ; genetics ; pathology ; Polycystic Kidney, Autosomal Recessive ; diagnosis ; genetics ; pathology ; Prenatal Diagnosis ; Receptors, Cell Surface ; genetics ; Tomography, X-Ray Computed

Acupuncture Points ; Acupuncture Therapy ; history ; China ; History, Ancient ; Humans ; Medicine in Literature

Objective To investigate the expression and implication of p21~(WAF1) and Ki-67 in the le- sions of Bowen Disease.Methods p21~(WAF1),Ki-67 proteins were examined with immunohistochemical SP method in 35 cases Bowen disease and 12 cases of normal skin or muscosa.Results Both of the expression of p21~(WAF1) protein and Ki-67 protein were elevated in Bowen disease lesions(both P

Red cell concentrates (Hct≥90%) weresuspended in SAGS medium(RCS.contain-ing sodium chloride,adenine,glucose,sucrose),and in H(?)gman's SAGM me-dium (containing sodium chloride,adenine,glucose,mannitol).Both were stored at4?2℃ for 35 days and compared withbiochemical tests.The results of posttrans-fusion survival,ATP,2,3-DPG,pH,K~+ tests between the two preparationswere similar,and the mean values ofinvitro hemolysis of SAGS-RCS was signi-ficantly lower than that of SAGM-RCS,i。e.1.71?0.95g/L and 3.10?0.19g/L res-pectively (P

Clinical Medicine ; Humans ; Neoplasms ; prevention & control ; Periodicals as Topic ; Research

Hepatocyte growth factor(HGF) is a mesenchyme-derived pleiotropic factor which regulates cell growth,cell motility,and morphogenesis of various types of cells,and is thus considered a humoral mediator of epithelial-mesenchymal interactions responsible for angiogenesis,apoptosis and oxidative stress,etc.This article reviewed the relationships between HGF and cardiovascular diseases.

OBJECTIVE To study the effect of Cistanches phenylethanol glycosides (CPhGs) on acute lung injury in rats and the possible mechanism of action. METHODS Sixty SD rats were randomly divided into six groups: Normal control group, model group (lipopolysasccharide (LPS) 3 mg·kg-1], dexamethasone (Dex) positive control group (model+ Dex 2 mg·kg-1), model + CPhGs 125, 250 and 500 mg·kg-1 with 10 rats in each group. Each dose group of CPhGs was ig administered every day for 30 d, and the other three groups were ig administered with the same volume of distilled water for 30 d. The mode+Dex 2 mg·kg-1 group was ig given Dex 2 mg·kg-11 h before modeling. The rat model of acute lung injury was established after the rats in the other groups were intubated by the breath tube. Three hours after modeling, the rats were sacrificed by blood sampling from the abdominal aorta. The percentage of neutrophils in the whole blood, the activity of serum superoxide dismutase (SOD), glutathione (GSH) activity and the contents of malondialdehyde (MDA) and nitric oxide (NO) were detected. The contents of tumor necrosis factor α (TNF-α), interleukin-1β (IL-1β) and IL-6 in alveolar lavage fluid were detected. The wet/dry mass ratio (W/D) of the middle lobe of the right lung was calculated and the histopathological changes of the posterior lobe of the right lung were observed by HE staining. RESULTS Compared with normal control group, the percentage of neutrophils in the whole blood, W/D in the middle lobe of the right lung, the contents of MDA and NO in the serum were increased (P<0.05), but SOD, GSH, TNF-α and IL-6 were decreased in the model group (P<0.05). Compared with the model group, the percentage of neutrophils in the whole blood and W/D in right middle lobe of the right lung in the group of model+CPhGs 500 mg·kg-1 was decreased (P<0.05), but the contents of TNF-α and IL-6 in alveolar lavage fluid of rats decreased significantly, and histopathological observation showed that inflammation was alleviated to varying degrees. The activities of SOD and GSH in rat serum of the model + CPhGs 500 mg·kg-1 group were increased significantly (P<0.05), while the contents of MDA and NO were decreased significantly (P<0.05). CONCLUSION CPhGs have a protective effect against acute lung injury induced by LPS in rats, and its mechanism may be related to its antioxidant effect, alleviation of pulmonary edema and reduction of the release of inflammatory factors.

Acupuncture Points ; Acupuncture Therapy ; history ; China ; History, Ancient ; Humans

Objective To investigate the role of nuclear factor ?B activation in the onset of acute coronary syndrome(ACS).Methods 76 patients with acute myocardial infraction(AMI),41 patients with unstable angina pectoris(UAP),43 patients with stable angina pectoris(SAP)and 20 normal controls were enrolled.NF-?B activation in monocytes in peripheral blood monocyte was determined by ELISA with the NF-?B p65 Kit the at 3 and 5 days after admission.Results The activity of NF-?B in monocytes of peripheral blood in AMI patients and UAP patients was significantly higher than that in SAP patients and normal controls(P