Objective To evaluate the expression and its clinical significance of serum LCN-2 and MMP-9 in human breast cancer.Methods 96 patients with breast cancer,42 patients with benign breast diseases and 27 healthy controls were recrui-ted in this study.The LCN-2 and MMP-9 levels were measured by ELISA in the serum of 96 patients with breast cancer,42 patients with benign breast disease,and 27 health controls.Results The level of serum LCN-2 in the breast cancer group (89.76±28.38 ng/ml)was significantly higher than those in benign breast diseases (56.44±21.15 ng/ml)and healthy controls (44.32±16.84 ng/ml)(P<0.05).The level of serum MMP-9 in the breast cancer group (96.34±23.26 ng/ml) was significantly higher than those in benign breast diseases (28.16±11.57 ng/ml)and healthy controls (23.54±13.61 ng/ml)(P<0.05).No obvious difference in the levels of serum LCN-2 and MMP-9 was observed between benign breast disease and healthy controls (P>0.05).Correlation analysis showed that a positive correlation was found between the levels of serum LCN-2 and MMP-9 in breast cancer patients (r=0.513 5,P<0.05).The levels of serum LCN-2 and MMP-9 were significantly associated with tumor size,lymph node metastasis,the expression of ER and PR,TNM stage,but were not asso-ciated with tumor differentiation (P>0.05).The correlation analysis showed that the LCN-2 level was positive correlated with the MMP-9 level (P<0.05).Conclusion LCN-2 and MMP-9 could play a role in the tumorigenesis and development of breast cancer.Measuring of serum LCN-2 and MMP-9 may be a useful tool for diagnosis,effect monitoring and prognosis in breast cancer.

Objective To observe the therapeutic effect of Qiyao Xiaoke Capsule(Capsule for Diabetes) on the diabetes type Ⅱ.Methods Totally 240 diabetes type Ⅱ patients were randomized into a treatment group(n=150),and a control group(n=90);and other 30 diabetes patients who didn't regularly take hypoglycemic drugs were set as opening group.The treatment group was administered Qiyao Xiaoke Capsule plus Metformin Hydrochloride Tablets,while the control group was prescribed Xiaokeling Capsule in addition to Metformin Hydrochloride Tablets,and the opening group was given Qiyao Xiaoke Capsule only,with a leading period of two weeks.The patients were treated for 4 weeks.Before treatment and at the end of the 4 weeks' treatment,the fasting blood glucose(FBG),2 hours postprandial blood glucose(2hPG),and glycosylated hemoglobin(HbA1c) of all groups were determined,and scores of TCM syndromes and symptoms were observed to evaluate the therapeutic effect.Results The total effective rate of the treatment group was 74.67%,of the control group 72.22%,and in the opening group 70.00%.Except for the 2hPG of the opening group,the FBG,2hPG and HbA1c of 3 groups were decreased(P

OBJECTIVE: To optimize the preparation technology of Buqi tongmai capsule.METHODS: Orthogonal experiment of exeracting Radix Astragali was conducted with crude drugs soaking time,extracting times,amount of water added and decocting duration as factors and the content of astragaloside as index.Orthogonal experiment of exeracting Salvia miltiorrhiza,Ligusticum chuanxiong,Fructus Aurantii Immaturus,Carthamus tinctorius was conducted with crude drugs soaking time,amount of water added and decocting duration as factors and the content of tanshinol as index.RESULTS:Water was adopted for extraction and the concentration of alcohol for precipitate was 70 percent.The optimal technology of exeracting Radix Astragali was to soak the crude drugs for 30 min,decoct for 90 min in 10 times of water for 2 times.The optimal technology of exeracting S.miltiorrhiza,L.chuanxiong,Fructus Aurantii Immaturus,C.tinctorius was to decoct the crude drugs for 90 min,8 times of water for 2 times.CONCLUSION:The preparation processes is feasible,stable and meet the demands in the clinic.

Cells, Cultured ; Endothelial Cells ; drug effects ; metabolism ; Endothelium, Vascular ; cytology ; metabolism ; Gene Expression Profiling ; Humans ; Ouabain ; pharmacology ; Umbilical Veins ; cytology

OBJECTIVE: To investigate the pharmacokinetics of cefazedone sodium in Chinese healthy volunteers after a single or multiple dose of intravenous drip infusion. METHODS: Thirty healthy volunteers, half male and half female, were randomly assigned to three groups of single dose and one group of multiple dose. The plasma drug concentrations after intravenous drip of single dose of 0.5, 1.0, 2.0 g and multiple dose of 1.0 g cefazedone sodium were determined by HPLC-MS/MS. RESULTS: The pharmacokinetic parameters of cefazedone sodium after single dose of 0.5, 1.0, 2.0 g were obtained as follows: t1/2z were (1.85 ± 0.53), (1.81 ± 0.29), (2.07 ± 0.32) h, respectively; AUC0-t were (145.30 ± 68.67), (244.26 ± 38.284), (513.10 ± 127.27) mg · h · L-1; AUC0-∞ were (147.89 ± 70.45), (246.60 ± 40.61), (521.02 ± 134.33) mg · h · L-1; tmax were (0.30 ± 0.06), (0.33 ± 0.00), (0.35 ± 0.05) h; ρmax were(87.18 ± 36.37), (151.13 ± 19.02), (271.94 ± 38.86) mg · L-1; Vz were (9.97 ± 2.91), (10.67 ± 1.27), (11.75 ± 1.78) L, CLz were (3.88 ± 1.239), (4.14 ± 0.57), (4.03 ± 0.86) L · h-1, respectively. The multi-dose pharmacokinetic parameters of cefazedone sodium were obtained as follows: AUCss was (208.93 ± 37.37) mg · h · L-1; AUC0-t was (208.93 ± 37.37) mg · h · L-1; AUC0-∞ was (210.64 ± 38.55) mg · h · L-1; tmax was (0.33 ± 0.00) h; ρmax was(130.20 ± 22. 83)mg · L-1; ρss was (17.41 ± 3.11) mg · L-1; ρmin was (0.89 ± 0.60) mg · L-1; DF was (7.45 ± 0.70); t1/2z was(1.77 ± 0.25) h; Vz was (12.40 ± 2.57) L; CLz was (4.90 ± 0.95) L · h-1. CONCLUSION: Cefazedone sodium displays linear pharmacokinetics in the dose range of 0.5 to 2 g after single intravenous drip injection. No significant differences between genders are observed. The differences of the main pharmacokinetic parameters of tmax, t1/2z, Vz and CLz between each group are not significant (P > 0.05). There is no significant accumulation of cefazedone sodium in healthy volunteers after multiple intravenous drip injection. Copyright 2012 by the Chinese Pharmaceutical Association.

Objective To perform literature search and review on the controversial relationship between therapies of hyperthyroidism due to Graves'disease(GD)and the course of Graves'ophthalmopathy(CA)).Methods We searched the database of MEDLINE(1966-2006.3),EMBASE(1984-2005),Cochrane Library(2006 No.1),CBMdisc(1978.1-2006.4)and CNKI(1994-2006).The methodological quality of the studies selected for review was assessed according to the quality assessment criteria suggested by the Cochrane systematic review guideline.Meta-analysis was performed by RevMan 4.2 software.Results Eight studies were included in the systematic review.Meta-analysis showed that there was statistically significant difference between 131I and other forms of therapy[surgery or antithyroid drugs(ATD)](test value:2.31,5.97,3.70,5.55;all P＜0.05)in aggravation of exophthalmos and symptom improvement in patients without receiving thyroxine during the early stage to prevent hypothyroidism.However,there was no statisti cally significant differenee in the above relationship between surgery and ATD therapy in those patients already receiving thyroxine supplement(test value:0.27,0.99;all P＞0.05).There were not yet any studies on the impact between early prevention of hypothyroidism after 131I therapy and GO.Conclusions Based on meta-analysis on literature data,if early measures are not performed to prevent hypothyroidism after 131I therapy,it may induce or aggravate GO more frequently than ATD or surgical treatment.Symptomatic relief of GO after 131I therapy is also less effective than the other 2 forms of therapy.Therefore.131I therapy should be delivered carefully in those patients with GO.

Resident or specialist training plays a very important role in developing qualified clinicians and improving medical quality.In combination with the status quo of resident or specialist training in our country , brief analysis has been made on the characteristicsof military resident or specialist training .As a result, the 3 +X +militarymode has been put forward in military resident orspecialist training.According to Specialist Training Standards by National Health and Family Planning Commission , training criteria upto military requirements have been established in order to achieve systematic military residency , develop medical support capability andpromote military health development.

Objective To get a view of the participation, motivation and barrier of voluntary activities in medical graduates, and to provide valuable advices for improving such behaviors. Methods The convenience sample method was used in medical graduates in 2014 and the total sample was 348. The data of voluntary service were collected by self-designed questionnaire, including whether partici-pating in voluntary service, times, types, motivations and barriers. SPSS 20.0 were used in statistic analysis and data were processed by description analysis and χ2 test. Results The effective response rate is 80.7%(281). The proportion of the post graduated students participation in voluntary activities is 65.8%(185), however, only 13.4%(28) claimed such activities more than 3 times. The proportion of participation in male, student leaders and party members were higher than those female, non-student leaders and non-party members, the different is statistically significant (P<0.05). The proportion of medical related voluntary activities is 52.6%. The primary motivation of voluntary behaviors is social responsibility (30.1%), while the primary obstacle is lack of medium (39.50%)and limitation of personal time and energy (30.0%). Conclusions The participation and persistence of voluntary activity in graduated students are not high enough. The colleges need to strength the organization and management of voluntary activities and focus on social responsible education, inspire and guide the postgradu ate students to participate in voluntary activities persistently by using their specialties.

The serum level of cholygylycine acid was determined in 118 patients with liver cirrhosis and the patients were followed up for 1 year.37 patients out of the 118 died in the fol-lowup period.It was found that the serum level of cholygylycine acid was more closely correlated with the mortality rate of the patients with liver cirrhosis than other commonly used indices such as hepatic encephalopathy,ascites.nutritional status,hihrubin,albumin and gamma-globulin.The serum level of cholygylycine acid was compared with Child's grading concerning the prediction of the mortality rate.After Logistic regression analysis.it was confirmed that the serum level of cholygylycine acid was the most ideal index for the prognosis of the patients with liver cirrhosis.

Objective To study the change of maternal serum human chorionic gonadotropin(MShCG) in preterm labor and the relationship between hCG and placental pathological changes. Metheds The preterm labor patients were chosen as the research subjects, the normal late-pregnancy women with the same gestational age were chosen as controls. MShCG level were measured by radioimmunoassy (RIA), placental tissue of preterm labor women was examined histologically. hCG in placental tissue was examined by semiquantiative immunohistochemistry. Results MShCG in preterm labor was significantly higher than that of control P