As the core of traditional Chinese medicine theory, Zheng (syndrome, or pattern) classification will promote personalized medicine by changing the clinical diagnosis into a more precise mode when integrating Zheng classification with disease diagnosis approaches. The author adopted rheumatoid arthritis (RA) as a disease model, to explore the scientific fundamentals of Zheng classification based on disease diagnosis using systemic biological approaches and evidence-based medicine design, as well as developed novel approaches on the methodology of clinical effectiveness evaluation on Chinese medicine and R&D of combinational drugs design based on Fu Fang (Chinese herbal formula). Some unique research design and methods are herein introduced.
Anti-Infective Agents ; Drug Combinations ; Drug Discovery ; Drugs, Chinese Herbal ; classification ; Evidence-Based Medicine ; Humans ; Medicine, Chinese Traditional ; Nutrition Therapy ; Precision Medicine ; Syndrome ; Treatment Outcome

Objective:To investigate the expression and distribution of aquaporin 5(AQP5) in allergic rhinitis(AR)treated by fluticasone propionate and levocabastine.Method:Forty Wister rats were divided randomly into AR(n=30) and control groups(n=10). After AR models were established, the AR rats were divided evenly into F group, L group and AR control group.Three groups were treated respectively for 28 days, then the expression of AQP5 in nasal mucous membrane were detected by immumohistochemistry assay.Result:The distribution of AQP5 was consistent in all groups. The expression of AQP5 in F group was significantly different from L group and AR group (P < 0.05), while there was no significant difference between that of AR group and L group(P>0.05). The expression of AQP5 in L group was significantly different from that in control group(P<0.05).Conclusion:High expressions of AQP5 in rat with AR indicated the positive correlation between AQP5 and AR. AQP5 might be one of pathological factors of AR concered with gland's excessive secretion and tissue edema. Glucocorticoid can down-regulate the expression of AQP5,but H1-receptor antagonist can not reduce the expression of AQP5.

To report a case of laryngopharyngeal recess fibroma with the clinical and pathological characteristics. The laryngopharyngeal recess neoplasm was expect with pedestal laryngoscope. The postoperative pathologic diagnosis was laryngopharyngeal recess fibroma. The tumor did not recurred after one year following-up. Surgery is the first selection for treatment of patient with laryngopharyngeal recess neoplasm. A closed follow-up is needed.
Female ; Fibroma ; diagnosis ; surgery ; Humans ; Laryngeal Neoplasms ; diagnosis ; surgery ; Middle Aged

Enamel hypoplasia is a surface defect of the tooth crown caused by a disturbance of enamel matrix secretion.Enamel hypoplasia may be inherited,or result from illness,malnutrition,trauma,or high concentrations of fluorides or strontium in the drinking water or food.Different types of enamel hypoplasia have been distinguished,such as pit-type,plane-type,and linear enamel hypoplasia.Hypoplasia has been related to the intensity and duration of stress events,the number of affected ameloblasts,and their position along the forming tooth crown.Amelogenesis imperfecta(AI) is a heterogeneous group of inherited defects in dental enamel formation,most teeth are affected in both the primary and permanent dentition.The malformed enamel can be unusually thin,soft,rough and stained.The strict definition of AI includes only those cases where enamel defects occur in the absence of other symptoms.Currently,there are seven candidate genes for AI: amelogenin,enamelin,ameloblastin,tuftelin,distal-less homeobox 3,enamelysin,and kallikrein 4.Since the enamel is formed according to a strict chronological sequence,and once formed,undergoes no repair or regeneration.Then the analysis the phenotype of enamel hypoplasia can provide insights of the severity of inherited or environmental stress and the molecular mechanism during the period of enamel formation.

Accidents, Occupational ; Humans ; Hydrogen Sulfide ; poisoning

The property theory of Chinese herbal medicine (CHM) is regarded as the core and basic of Chinese medical theory, however, the underlying mechanism of the properties in CHMs remains unclear, which impedes a barrier for the modernization of Chinese herbal medicine. The properties of CHM are often categorized into cold and heat according to the theory of Chinese medicine, which are essential to guide the clinical application of CHMs. There is an urgent demand to build a cold/heat property classification model to facilitate the property theory of Chinese herbal medicine, as well as to clarify the controversial properties of some herbs. Based on previous studies on the cold/heat properties of CHM, in this paper, we described a novel strategy on building a cold/heat property classification model based on herbal bio-effect. The interdisciplinary cooperation of systems biology, pharmacological network, and pattern recognition technique might lighten the study on cold/heat property theory, provide a scientific model for determination the cold/heat property of herbal medicines, and a new strategy for expanding the Chinese herbal medicine resources as well.
Drugs, Chinese Herbal ; chemistry ; therapeutic use ; Herbal Medicine ; Humans ; Phytotherapy ; Plants, Medicinal ; chemistry

OBJECTIVE To estimate the value of Mycoplasma pneumoniae(Mp)-IgM,C-reactive protein(CRP) and WBC on diagnosis of children respiratory infection.METHODS Serum Mp-IgM antibody,CRP and WBC were tested in 225 children with respiratory infection by passive particle agglutination,ITM immunoturbidimetry and analyzer of blood cell.RESULTS Forty-six cases of 225 children were identified positive in Mp-IgM.The positive rate of Mp-IgM was 20.4%.Compared the CRP and WBC among cases positive in Mp-IgM,bacterial infected and virus infected,there was significant difference bewteen cases positive in Mp-IgM and bacterial infected,no significant difference bewteen cases positive in Mp-IgM and virus infected.CONCLUSIONS The joint detection of serum Mp-IgM antibody,CRP and WBC can be a valuable clue to diagnosis of children respiratory infection.

Accidents, Occupational ; Adolescent ; Adult ; Humans ; Male ; Middle Aged ; Organotin Compounds ; poisoning ; Young Adult

objective To analyze the risk factors of sepsis,and to elucidate the efficacy of high-flux hemofihration(HVHF)in the treatment of sepsis and the initiate opportunity of HVHF treatment on sepsis complicated with acute kidney injury(AKI). Methods Clinical data of 152patients with sepsis undergoing HVHF treatment were retrospectively analyzed.APACHE Ⅱ,APACHE Ⅲ and SAPS Ⅱ score evaluation before treatment and 24 h,48 h.72 h after treatment were performed.According to 28-day survival,patients were divided into survival group and death group.Clinical indicators before and 24 h,48 h,72 h after treatment were compared between groups.Risk factors influencing prognosis of patients were examined by multivariate regression analysis.Influence of AKI stages on prognosis of patients was studied. Resalts Lung and alimentary tract were the most frequent infection sites of sepsis.Twenty-eight days after HVHF treatment,74 patients (48.68%)survived,seventy-eight patients (51.32%)died among 152patients,and 4 patients(2.63%)needed sequent renal replacement therapy.Twenty-eight-day mortality was significantly lower than the mortality 88.78%±17.72%predicted by APACHE Ⅲ.APACHE Ⅱ,APACHE Ⅲ and SAPS Ⅱ Sscores decreased remarkably (P＜0.01) 24 hours after treatment.Age,number of dysfunctional organ,APACHE Ⅱ,APACHE Ⅲ,SAPS Ⅱ scores,undedying diseases,shock,AKI,severe acute pancreatitis,alimentary tract hemorrhage,cataphora and pressor agent were correlated with 28-d survival rate.Underlving diseases and APACHE Ⅲ were independent risk factors.Renal function recovery rate in AKI stage 1 was higher than that in AKI stage 2,3(P＜0.05).Twenty-eight-day mortality of patients without AKI was lower compared to AKI stage 1,2,3(P＜0.05),but 28-day mortality was not significantly different among AKI stage 1,2,3. Conclusions Prognosis of patients with sepsis is poor.HVHF can improve biochemical indicators and prognosis of patients effectively,and increase survival rate.Underlying diseases and APACHE Ⅲ are independent risk factors of 28-day mortality of sepsis patients.Twenty-eight-day mortality of non-AKI patients is obviously lower compared to AKI stage 1,2,3patients.AKI stage is correlated with 28-day renal function recovery rate,but not obviously correlated with 28-day mortality.

In order to safeguard the legitimate rights and interests of the subjects and the interests of the whole society , a relaxed and harmonious environment was created to ensure the normal medical research , clini-cal research trials which fit in trial of ethical review. The protocol assessment form and ICF assessment form to review are two cornerstones of protecting subjects. A good ICF assessment form is a necessary means to help improve the quality of the Ethics Committee for review .