Objective Investigate the clinical significance of bone marrow minimal residual disease (MRD) detection to prognosis and therapeutic evaluation in acute leukemia (AL) after complete remission (CR). Methods Forty-three cases of bone marrow specimens in patients with acute leukemia were collected from 209-2012. The MRD was detected using multiparameter flow cytometry (FCM). The detection was performed from the beginning of induction chemotherapy by CR,and detected at 1,3,6 and 12 months. According to the CR detection for the first time, the induced MRD levels were obtained after treatment, then 43 patients with AL were divided into negative (MRD<10-4) and positive (MRD>10-4) two groups. Results The recurrence rate after three years of the two groups was 0 and 80%, respectively. There was a significant difference in the recurrence rate ( <0.05) . The recurrence rate of positive group was significant higher than that of negative group ( <0.05) when the morphologic of MRD was complete remission. Conclusion Using FCM to detect AL MRD, MRD>10-4 AL-CR have the high recurrence rate, and the recurrence of positive group have appeared early than traditional MRD bone marrow morphology. The continuous MRD detection of AL-CR has important clinical significance in the prognosis and early prediction for recurrence.

Objective To investigate the effect of intensive nursing intervention on the secondary infection in chemotherapy of elderly patients with lung cancer.Methods 168 cases of elderly lung cancer patients whose neutrophil ≤ 1.0×109/L during chemotherapy were randomly divided into two groups,namely,the intensive care group and the control group,with 84 cases in each group.The control group was given routine nursing of infection prevention,while the intensive care group was given intensified nursing for infection prevention.A contrastive analysis was conducted in terms of the incidence,clinical characteristics and the duration of antibiotic treatment of the two groups of patients with secondary infection.Results The secondary infection rate of the intensive care group was lower than that of the control group during chemotherapy.What's more,the duration of fever and treatment of the intensive care group was shorter than that of the control group,hence the patients were safe during infection period.The difference was statistically significant.Conclusions Strengthening the nursing intervention can reduce the incidence rate of secondary infection in aged patients with lung cancer,increase the tolerance of patients to chemotherapy and ensure the successful completion of the chemotherapy cycle,prolong the life span of the patients with an improved quality of life.

Arthritis, Rheumatoid ; classification ; diagnosis ; drug therapy ; Diagnosis, Differential ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Humans ; Male ; Medicine, Chinese Traditional ; methods ; Phytotherapy ; methods ; Syndrome

OBJECTIVE:To control the drug quality risk in drug withdrawal links of hospital pharmacy,and provide reference for the application of quality risk management(QRM)in hospital pharmacy. METHODS:Drug QRM was developed in drug with-drawal links through risk identification,risk assessment,risk control,risk assessment and other steps. Using the incidence of risk factors and reusable rate of withdrawal drug as indexes,related data of before(Jul.-Dec. 2015)and after(Jan.-Jun. 2016)devel-oping QRM in our hospital were compared to evaluate the effect of drug QRM. RESULTS:Determining whether specific storage drugs kept the required storage conditions,whether the numbers of withdrawal drugs counted to minimal packaging and other 5 fac-tors were high-risk factors(the risk score of each factor>4 points);control measures were taken separately for high risk factors, then high-risk factors were reduced to acceptable levels(risk score<4 points). After developing QRM,the incidence of risk factors were reduced than before(reduce 1.35%-6.19%),reusable rate of withdrawal drug was increased(98.64% vs. 86.32%)(all P<0.05). CONCLUSIONS:Developing QRM in drug withdrawal links of hospital pharmacy can reduce the drug quality risk.

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OBJECTIVE:To investigate the effects of Dexmedetomidine hydrochloride injection on elderly patients underwent thoracic surgery during anesthesia recovery period. METHODS:Totally 60 elderly patients underwent thoracic surgery in our hospi-tal during Jun. 2014-Feb. 2016 were selected as research objects and then divided into control group and observation group ac-cording to even and odd-numbered admission order,with 30 cases in each group. Control group was given Propofol injection 0.5-1 mg/kg intravenously 5 min after mask oxygen inhalation. Observation group was given Dexmedetomidine hydrochloride injec-tion 0.5-1.0 μg/kg intravenously. Both group were successively given Etomidate emulsion injection 0.1-0.2 mg/kg,Sufentanil citrate injection 0.3 μg/kg and Cisatracurium besilate for injection 0.2 mg/kg intravenously for anesthesia induction;tracheal intubation was performed and ventilator was used to assist ventilation after anesthesia induction. And then control group was given continuous intravenous dripping of propofol 3-6 mg/kg·h;observation group was given continuous intravenous dripping of Dexmedetomidine hydrochloride injection 0.2-0.5 μg/(kg·h). At the same time,both group were given continuous intravenous dripping of sufentanil 0.1-0.2 μg/(kg·min)and cis-atracurium 1.5-2 μg/(kg·min),and continuously inhaled 1%-2% sevoflurane for anesthesia mainte-nance. Hemodynamic index(HR,MAP,SpO2),postoperative recovery,the occurrence of delirium,restlessness,cough and involun-tary movement were observed in 2 groups before surgery (T0),after anesthesia induction (T1),at the moment of sputum suction and extubation(T2),5 min after extubation(T3),30 min after extubation(T4). RESULTS:At T2 and T3,HR and MAP of control group were increased significantly compared to at T0,T1,and higher than those of observation group,with statistical significance (P<0.05). At T0,T1,T4,there was no statistical significance in HR and MAP between 2 groups (P>0.05). At T0,T1,T2,T3, T4,there was no statistical significance in SpO2 level between 2 groups(P>0.05). The breathing recovery time,eye openning time and extubation time of observation group were significantly shorter than those of control group,with statistical significance (P<0.05). The incidence of postoperative restlessness,delirium,cough and involuntary movement in observation group were signifi-cantly lower than control group,with statistical significance (P<0.05). CONCLUSIONS:Dexmedetomidine hydrochloride injec-tion can keep hemodynamics stable in elderly patients underwent thoracic surgery during anesthesia recovery period.,shorten recov-ery time and reduce the occurrence of stress response as postoperative delirium,restlessness,cough,involuntary movement.

Genome sequencing and bioinformatics is driving the discovery of drug targets and development of novel classes of broad spectrum antimicrobial compounds. The analysis of the complete genome sequences of microorganism available in the public databases offers the first insights into the drug targets discovery. This review discusses the use of genomic information in the finding of target genes for antimicrobial drug discovery.

Objective To explore the effect of family health management on community type 2 diabetes medication compliance.Methods Totally 123 patients with type 2 diabetes who live in Shanghai City Qingpu District Xujing Town An Ju Wei Hao Xiao Qu and met the WHO diagnostic criteria for type 2 diabetes were enrolled into this study.The patients' age was 50-75 years,including 60 male,63 female cases,mean age was (63.9 ± 6.9),(10.0 ± 7.4) years,course of disease,a family health file,making personalized home health management measures of community were analyzed.The effects of the 2 years of intervention on the compliance of the patients before and after the intervention as well as the blood pressure,blood glucose,blood lipid control were evaluated.Results The treatment compliance rate was (77.1％,94/122,intervention 1 cases died) significantly higher after intervention than that before intervention (62.6％,77/123) (x2=6.06,P＜0.05).After the intervention,the rate of target achievement in blood pressure (51.6％,63/122),blood glueose (44.3％,54/122),total cholesterol (72.1％,88/122),triglyceride (61.5％,75/122) low density lipoprotein (75.4％,92/122) were higher than those before intervention [blood pressure standard rate of 36.6％ (45/123),blood glucose standard rate of 28.5％ (35/123),total cholesterol standard rate of 59.3％ (73/123),triglyceride standard rate of 47.9％ (59/123),low density lipoprotein standard rate of 65.0％ (80/123)],the differences were statistically significant (x2=5.63,6.61,4.44,4.51,3.97,P＜0.05).Conclusion Community and family health management can improve the medication compliance in patients with type 2 diabetes.

The purpose of the medical moral education is to make medical undergraduates form stable feature and tendency of the medical moral psychology.In order to improve the development of the medical moral psychology as well as ensure the result of medical moral education,we should continuously make the medical moral education suit to the rule of the formation and development of medical moral psychology.

Objective To explore the biological features of hematopoiesis reconstitution by using genetic marking.Methods NeoR gene was transduced into bone marrow(BM)cells of mouse mediated by liposome.Then these cells were transplanted into lethally irradiated recipients.The hematopoiesis reconstitution was observed and the marker gene in spleen and BM cells of recipients after hematopoiesis reconstitution was examined.Results The transplanted recipients remained alive and healthy.But the irradiated mice with no transplantation died from BM aplasia soon.Meanwhile,the cells from spleen and BM of transplanted mice could be alive in G418 system,and contained the DNA fragments of NeoR gene by PCR.Conclusion Gene-modified BM cells could be used to reconstitute hematopoiesis successfully and express the foreign gene to some extent stably.