Brain Abscess ; etiology ; Female ; Humans ; Leuconostoc ; pathogenicity ; Male ; Sepsis ; complications ; microbiology

Objective The purpose of this study was to determine and compare the hemodynamic effects of desflurane and enflurane in children.Methods Twenty-eight ASA Ⅰ-Ⅱ patients, aged 1-5 years were randomly assigned to receive either desflurane (group D) or enflurane (group E) for maintenance of anesthesia.Cardiac output was continuously monitored by transesophageal Doppler (TECO1).The stroke volume index (SVI), cardiac index (CI),heart rate (HR) and blood pressure(SBP,DBP,MAP) were determined before and during inhalation of desflurane and enflurane at 0.5,1.0 and 1.5 MAC.Results At 0.5 MAC of both agents SVI, CI and HR remained unchanged, but SBP, DBP and MAP were slightly reduced as compared to the baseline values.At 1.0 MAC of desflurane CI was increased and was higher than that in the group E. SVI and HR were significantly higher in group D than those in group E at 1.0 MAC.SBP, MAP and DBP were further reduced and the magnitude of reduction was similar between both groups.At 1.5 MAC CI and SVI were unchanged in group D but greatly reduced in group E.HR increased significantly in group D but remained unchanged in group E.There was further decrease in BP in both groups but SBP and MAP decreased more markedly in group E than in group D .Conclusions Desflurane causes less hemodynamic changes in children of 1-5 years than enflurane.

Objective To investigate the change in heat shock protein 70(HSP7O) in the brain tissue of fetal and newborn rats after prenatal hypoxic adaptation and the possible mechanism of the protective effect. Methods Twenty-two 22d pregnant Wistar rats were randomly divided into two groups: GroupⅠ(hypoxic adaptation group) and group Ⅱ(control group). The animals in group Ⅰ were placed in a tightly closed hypoxic adaptation chamber, of which the oxygen and carbon dioxide concentrations were monitored. The pregnant rats were taken out and exposed to fresh air for 5 mm when the 02 % in the chamber was reduced to 15 %, then the pregnant rats were placed back in the chamber and the above process was repeated once. The animals were then left for their natural labor. In control group the pregnant rats underwent the same process but the chamber was not tightly closed(O2 %= 21 %). Prenatal rats were delivered by cesarean section at lb. 3h, 8h, 24h, 48h, 72h, 120h and 168h after hypoxic adaptation and decapitated and brain was removed. Seven newborn rats from each group were decapitated and brain was removed for determination of HSP70 expression with immunohistochimical technique. Results No HSP7O expression was observed in the brain tissue of normal prenatal and newborn rats. HSP70 was observed in the different regions of hippocampus and cortex from 8h to 168h after hypoxic adaptation. Strongest HSP70 expression was observed in hippocampus CAl . Conclusions HSP70 plays a role in the formation of prenatal hypoxic adaptation.

Adult ; Aged ; Aged, 80 and over ; Carbon Monoxide Poisoning ; complications ; Female ; Humans ; Interleukins ; blood ; Male ; Middle Aged ; Neurotoxicity Syndromes ; blood ; etiology

Objective To study the histocompatibility of domestic new type nitinol alloy ventricular septal defect(VSD) occluder in order to test the feasibility for clinical application.Methods The domestic new type nitinol alloy VSD occluder was inserted into 20 healthy dogs with intravenous balanced anesthesia and tracheal intubation,assisted by animal breathing machine respiration.The dogs were given oral antibiotic and would be killed in several months after operation.According to different slaughter time(1,2,3,6 months),they were divided into 4 sections and including 5 dogs in each section.The occluder sites,the intimal coverage and the change of inflammatory cells were observed through gross anatomy,with the help of the light microscope and electron microscope.SPSS 11.0 software was used to analyze the data.Results The thickness of the endocardial enhanced with the time(P

Aged ; Aged, 80 and over ; Female ; Humans ; Hyponatremia ; chemically induced ; Iodipamide ; adverse effects

Twenty-three histone methyltransferase genes were obtained from transcriptome dataset of Lonicera japonica. The nucleotide and proteins characteristics, subcellular localization, senior structural domains and conservative forecasting were analyzed. The result of phylogenetic tree showed that 23 histone methyltransferases were mainly divided into two groups: lysine methyltransferase and arginine methyltransferases. The result of gene expression showed that 23 histone methyltransferases showed preference in terms of interspecies and organs. They were more expressed in buds of L. japonica than in L. japonica var. chinensis and lower in leaves of L. japonica than in L. japonica var. chinensis. Eight genes were specific expressed in flower. These results provided basis for further understanding the function of histone methyltransferase and epigenetic regulation of active ingredients of L. japonica.
Computational Biology ; Gene Expression ; Histone-Lysine N-Methyltransferase ; genetics ; Lonicera ; enzymology ; genetics ; Phylogeny

Objective: To study the chemical constituents of phenylpropanoids and lignans in Selaginella involven and to explore their roles in the plants evolution of Selaginella P. Beauv. Methods: The chemical constituents were isolated and purified by polyamide, macroporous resin, and Sephadex LH-20 column chromatography, and their structures were elucidated by chemical and spectroscopic methods. Results: Fifteen compounds were identified as 3-(3-hydroxyphenyl)-propionic acid (1), 3-(3-hydroxyphenyl)-methyl propionate (2), cinnamic acid (3), 3-hydroxy-1-(3-methoxy-4-hydroxyphenyl) propan-1-one (4), 3-hydroxy-1-(3,5-dimethoxy-4-hydroxyphenyl) propan-1-one (5), (+)-wikstromol (6), (-)-nortrachelogenin (7), (+)-matrairesinol (8), (5H)-2-furanone-3,4-bis[(4-hydroxy-3-methoxyphenyl) methyl] (9), acutissimalignan B (10), detetrahydroconidendrin (11), syringaresinol (12), 3,3',5-trimethoxy-4',7-epoxy-8,5'-neolignan-7-ene-4,9,9'-triol-9-aldehyde (13), ciwujiatone (14), and tarennone (15), respectively. Conclusion: Compounds 1-15 are isolated from this plant for the first time. Among them 1-3, 9-11, and 13-15 are isolated from the plants of Selaginella P. Beauv. for the first time. The discovery of a series of mustards, coniferyl alcohols, and their derivatives, which is the representative of phenypropanoids and lignans from S. involven, plays an important role in the development of plants in Selaginella P. Beauv..

Rehabilitation is very important to the therapeutic effect of total knee arthroplasty, and it's also the key measure for the knee function to reach the expected effect after the surgery. The most important contents of rehabilitation therapies are the training of muscle strength, range of joint motion, joint position sense and gait. Integrated rehabilitation therapies was used to improve the knee function as possible, such as all kinds of training to improve the knee muscle strength of flex and extend; early training of continued positive motion of knee joint to improve the knee range of motion; the training of knee joint position sense to improve the ability of motion control, posture adjusting and balance keeping; the gait training to adjust the unusual gait.

ObjectiveTo study the effct of the early rehabilitation of acute stroke with acupunction added to rehabilitational training.Methods120 patients with acute stroke were divided into 2 growps with 60 cases each:the acupunction growp,in which the patients were acepted acupuncture added to rehabilitational training,and the control growp,in which the patients were acepted the rehabilitational training only.All the patients were evaluated with Clinical Nervous Functional Deficiency(CNFD),Fugl-Meyer Assessment(FMA) and Modified Barthel Index(MBI).Results The scales of CNFD,FMAanal Deficiency (CNFD),Fugl-Meyer Assess me that of the control growp.The rate of cure and significantly in the acupunction growp was 75%,while that of the control growp was 41.7%(P<0.01).Conclusions Acupunction compared with the rehabilitational training is effective on the with stroke.