Background In vitro study showed that chemotaxis consist of chemotaxis factor 4(CXCR4)and stromal cells derived factor-1(SDF-1)and may play a role in the orientation and migration of retinal progenitor cells (RPCs)toward lesion.Overexpression of CXCR4 in RPCs can enhance the chemotaxis activity.Objective This work was to explore the feasibility and underlying mechanism of up-regulation of CXCR4 on RPCs induced by hypoxia.Methods RPCs were retained in an incubator with normal O2volume(16％)or hypoxia condition(10％ O2)for 12 hours and 24 hours respectively.Flow cytometer cell analysis screening(FACS)was conduced to measure the proportion of CXCR4-expressing cells,and CXCR4,HIF-1 mRNA were analyzed by reverse transcription-polymerse chain reaction(RT-PCR).The chemotical effect of 30 mg/L SDF-1 to RPCs cultured under the hypoxia condition was assessed using Boyden chamber.Results The expression level of CXCR4(CXCR4 mRNA/β-actin mRNA)inRPCs cultured by 10％ O2 for 12 and 24 hours were 0.28+0.07and 0.48+0.17 and increased by 1.75 and 3.00 fold more than that of 16％ O2 culture group(0.16+0.02)(P＜0.01).The expression level of HIF-1 mRNA(HIF-1 mRNA/β-actin mRNA)in RPCs cultured by 10％ O2 for 12 and 24 hours were 0.18 ±0.07and 0.38 ±0.13 and increased by 3.00 and 6.30 fold more than that of 16％ O2 culture group(0.06±0.01)(P＜0.01).The chemotical effect of 30 μg/L SDF-1 to RPCs increased from 13.00％ in 16％ O2 culture group to 36.00％ and 46.00％ in the cells cultured by 10％ O2for 12 and 24 hours.FACS revealed that the proportion of CXCR4+ cells in hypoxia-exposure for 12 and 24 hours were 26.90％ and 46.10％,respectively,but that in 16％ O2 culture group was 9.10％,showing a statistically significant difference(P ＜ 0.01).Conclusions RPCs induced by hypoxia can enhance the expression of CXCR4 in RPE cells and the chemotaxia to SDF-1.The overexpression of H1F-1 in RPCs may be involved in the up-regulation of CXCR4 expression.

Animals ; Dementia, Vascular ; blood ; psychology ; Erythrocyte Count ; Learning ; drug effects ; Male ; Memory ; drug effects ; Peptides ; pharmacology ; Rats ; Rats, Sprague-Dawley

Objective To investigate the clinical application value of psychological induction in impression taking of deciduous crossbite.Methods Totally 84 children of deciduous crossbite receiving impression taking were divided equally into 2 groups.Children in the control group received impression taking directly,while children in the experimental group received psychological induction before impression taking.A comparative study was carried out according to special cooperative behavior assessment for children.Results The cooperation degree of the experiment group was increased significantly after psychological induction with statistical differences between them(P ＜ 0.05).Conclusions Psychological induction can improve the cooperation degree of children in impression taking.

Animals ; Ear Canal ; surgery ; Female ; Gene Expression ; Hypothalamus ; metabolism ; Nerve Block ; Pregnancy ; RNA, Messenger ; genetics ; Rats ; Rats, Sprague-Dawley ; Receptors, N-Methyl-D-Aspartate ; genetics ; metabolism

Animals ; Female ; Gene Expression Regulation, Developmental ; Inferior Colliculi ; metabolism ; N-Methylaspartate ; genetics ; metabolism ; RNA, Messenger ; genetics ; Rats ; Rats, Sprague-Dawley ; Receptors, N-Methyl-D-Aspartate ; genetics ; metabolism

OBJECTIVE:To monitor the antimicrobial resistance of Enterococcus faecalis and Enterococcus faecium in our hospital from 2006 to 2008 in order to provide reference for clinical rational use of antibiotics.METHODS:The susceptibility of isolated enterococci was determined by K-B paper diffusion method,and the antibiotic resistance rates of Enterococcus faecalis and Enterococcus Faecium were analyzed using Whonet 5 software.RESULTS:Vancomycin was the most sensitive antimicrobial against Enterococcus faecalis and Enterococcus faecium.E.faecalis had low resistance rates to penicillin G,high concentration gentamicin,ciprofloxacin and rifampicin,while E.faecium had low resistance rates to high concentration streptomycin,tertracycline,chloromycetin,and qinupristin/dalforpristin.CONCLUSION:E.faecalis and E.faecium are multi-drug resistant.Antimicrobials should be used rationally based on the susceptibility test of E.faecalis and E.faecium.

Objective To study the association between cytotoxin-associated gene A of Helicobacter pylori (Hp-CagA) and the expressions of mtp53 and c-myc protein in different subtypes of intestinal metaplasia.Methods One hundred and sixty-five patients with gastroscopy included 125 cases with chronic atrophic gastritis with intestinal metaplasia,40 cases with non-atrophic gastritis.Intestinal metaplasia included complete intestinal metaplasia,incompletetype intestinal metaplasia,complete colonic epithelial metaplasia and incomplete colonic epithelial metaplasia.The carbon-14-urea breath test was used to determine Helicobacter pylori (Hp);AB-PAS and HID-AB mucinous staining was used to distinguish intestinal metaplasia subtypes; the immunohistochemical Elivision method was used to determine the expressions ofmtp53 and c-myc protein;indirect ELISA was used to determine Hp-CagA.Results Forty-five cases with incomplete colonic epithelial metaplasia,47 cases with incomplete type intestinal metaplasta,17 cases with complete colonic epithelial metaplasia,16 cases with complete intestinal metaplasia.The positive rate of Hp-CagA in all intestinal metaplasia subtypes was higher than that in non-atrophic gastritis,but there was no significant difference (P ＞ 0.05).The expression of mtp53 protein in incomplete colonic type of intestinal metaplasia with Hp-CagA positive was higher than that in incomplete colonic type of intestinal metaplasia with Hp-CagA negative (x2 =5.494,P ＜ 0.05).The expression of c-myc protein in incomplete colonic type of intestinal metaplasia with Hp-CagA positive was higher than that in incomplete colonic type of intestinal metaplasia with Hp-CagA negative (x2 =13.950,P ＜ 0.01).Conclusion Hp-CagA is a sort of highly virulent strain,and Hp-CagA may do a strong role in the promotion of gastric atrophy and intestinal metaplasia.

Objective To study the relieving cough effect ofPuji-Xuanfei-Xiaodu decoction on acute bronchitis.Methods 87 patients with acute bronchitis of exterior cold and interior heat were randomly divided into two groups(44 cases in a therapy group and 43 cases in a control group).Puji-Xuanfei-Xiaodu decoction was given to the patients in the therapy group and acute bronchitis syrup was given to the control group, to observe the influence on symptoms of cough, sputum, fever and headache. The course of treatment was 7 days. Results On the reliving cough aspect, 28 cases (63.6%) showed cure effect and 13 cases (29.5%) effctive, the total effective rate was 93.2% in therapy group with 44 cases; while in control group (43 cases), 15 cases (43.9%) showed cure effect and 23 cases (53.5%) effctive, the total effective rate was 88.4%. The symptoms of cough, phlegm, fever, headache and aversion to wind were significantly decreased in two groups. In therapy group, the numbers of cure effect, remarkable effect and efficacy were 22 (50%) ,11(25%) and 8 (18.2%) cases respectively, the total effective rate was 93.19% while the numbers of cure effect, remarkable effect and efficacy were 14 (32.6%) ,8(18.6%) and 12 (27.9%) cases respectively, the total effective rate was 79.07% in control group. The total effective ratio of two groups had significant difference (P>0.05).ConclusionsPuji-Xuanfei-Xiaodu decoction is effective in relieving cough.

AIM: To investigate the changes of bladder volume , pressures and pelvic nerve firing induce by different speeds and ways of instillation .METHODS:Female SD rats (n=20) were randomly divided into 2 groups.The rats in group A was performed using 3-F polyethylene tubing inserted into the bladder through the urethra .The rats in group B were performed using 3-F tubing inserted into the dome of the bladder and secured by silk suture .The rats in both groups were infused with saline into the bladder at different speeds of 50, 100, 200 and 400μL/min.The changes of bladder vol-ume and pressure were recorded by urodynamic measuring devices .The changes of pelvic nerve firing during instillation were recorded by multi-channel physiologic recorder .RESULTS:In group A, the maximum firing frequency , bladder leak point pressure ( BLPP) and maximum voiding pressure ( MVP) were increased with the increase in the instillation speed . No significant difference of the maximum bladder capacity (MBC) at different speeds was observed .In group B, the maxi-mum firing frequency had no significant difference at different speeds .MBC was decreased with the increase in the instilla-tion speed , and exhibited significant decrease at 200 and 400 μL/min.No significant difference of BLPP and MVP at dif-ferent speeds was observed .Compared with group A , the maximum firing frequency and MBC in group B significantly de-creased at different speeds .However , BLPP and MVP in group B were higher than those in group A at the speeds of 50 and 100 μL/min.CONCLUSION:Different instillation speeds with the method of group A will not change the bladder volume but influence the pelvic nerve firing , so the process of method A may take various speeds according to different aims .How-ever, process of method B at the speed of over 200 μL/min may not be good to MBC, thus instillation under 200 μL/min is re-commended .

Objective To study the effect of psychological intervention combined with the analysis of Budesonide and Formoterol Fumarate Powder for Inhalation in treatment of chronic obstructive pulmonary disease. Methods 100 patients with chronic obstructive pulmonary disease treated in our hospital from February 2015 to March 2016 were selected and randomly divided into the control group and the experimental group, with 50 patients in each group. The control group was treated with the Budesonide and Formoterol Fumarate Powder for Inhalation treatment,the experimental group on the basis of psychological intervention on the mental status of patients and patients,strengthen communication and exchanges, increase confidence in the treatment and the treatment compliance of patients. Results After treatment, the SGRQ score of the experimental group was (57.33±16.81), and the score of the control group was (43.86±12.68) points, with statistical difference (P<0.05). The scores of depression and anxiety in the experimental group were significantly better than those in the control group,with statistical difference(P<0.05). Conclusion Budesonide and Formoterol Fumarate Powder for Inhalation combined with mental intervention in the treatment of chronic obstructive pulmonary disease can significantly improve the patients, improve the quality of life, help patients recover as soon as possible, has clinical significance.