OBJECTIVE:To establish a method for simultaneous determination for ginsenoside Rb1,ginsenosides Rc,ginsen-osides Rb2 and ginsenosides Rd in Shengmai powder. METHODS:HPLC was performed on the column of Agilent ZORBAX Ex-tend-C18 with mobile phase of acetonitrile-0.1% formic acid(gradient elution) at a flow rate of 1.0 ml/min,wavelength was 203 nm,column temperature was 30 ℃ and the volume injection was 10 ml. RESULTS:The linear was 0.78-38.75 μg for ginsenoside Rb1(r=0.9996),0.51-25.50 μg for ginsenosides Rc(r=0.9996),0.43-21.50 μg for ginsenosides Rb2(r=0.9997)and 0.20-10.00 μg for ginsenosides Rd(r=0.9997);RSDs of precision,stability and reproducibility tests were lower than 2%;recoveries were 96.6%-101.2%(RSD=1.6%,n=6),97.0%-102.1%(RSD=1.7%,n=6),99.1%-102.8%(RSD=1.3%,n=6)and 96.3%-101.1%(RSD=1.6%,n=6). CONCLUSIONS:The method is simple,rapid with good stability,reproducibility and high precision,and can be used for quality control of Shengmai powder.

Objective To evaluate t he efficacy of fluoxetine in treating tinnitus patients with depress. Methods A randomized,open-labeled,controlled clinical tri al was conducted.There were 31 patients in control group and 26 p a tents in fluoxetine group.The efficacy was assessed by tinnitus patients with Ra ting Scale and Hamilton Depression Rating Scale (HAMD). Results The total effective rate of fluoxetine group was 71.11%, and there was significant improvement rate in depression after 1 month f luoxetine treatm ent. Conclusion Fluoxetine is an effective drug for tinnitus p atients with depression.

This paper reports a case of recurrence of tonsillar cancer on the right 6 months after radiotherapy with pharyngeal hemorrhage for 4 days and aggravation in the next day. The pharyngeal hemorrhage was severe in the case and the maximum of single amount of bleeding was approximately 200 ml. The examination showed active bleeding on the ulcer with a diameter of 2 cm in the right tonsil and the depth of 1 cm. After repeated compression hemostasis proved to be invalid, selective embolization was applied on the patient and the symtoms of pharyngeal hemorrhage disappeared without complications. The patient was discharged after 2 weeks of observation without any recurrence of hemorrhage.
Embolization, Therapeutic ; methods ; Hemorrhage ; etiology ; therapy ; Hemostasis ; Humans ; Neoplasm Recurrence, Local ; Palatine Tonsil ; Pharyngeal Diseases ; etiology ; therapy ; Recurrence ; Tonsillar Neoplasms ; complications

Objective To examine the expression of macrophage migration inhibitory factor(MIF)in lung tissues of rats with experimental pulmonary fibrosis and to discuss its effects on pulmonary fibrosis.Methods36 male SD rats were randomly divided into two groups:the pulmonary fibrosis group and the control group.Rat pulmonary fibrosis was induced by an intratracheal injection of bleomycin.The following tests were undedaken: HE and Masson staining of lung section,immunohistochemical staining of protein of MIF in the lung,RT-PCR of MIFmRNA in the lung.ResultsCompared with the control group,the mRNA and protein expression of lung MIF on day 7 to 28 in pulmonary fibrosis increased and reached the peak on day 14 after an intratracheal injection of bleomycin(P

Alzheimer Disease ; immunology ; therapy ; Amyloid beta-Peptides ; antagonists & inhibitors ; immunology ; metabolism ; Animals ; Antibodies, Monoclonal ; therapeutic use ; Blood-Brain Barrier ; Brain ; drug effects ; immunology ; Enzyme Inhibitors ; therapeutic use ; Humans

OBJECTIVETo observe the effect of Pi transportation, dampness resolving and phlegm expelling herbs (PTDRPEH) on the obesity degree, fat hormones, and leptin resistance in diet-induced obesity (DIO) rats.

METHODSAmong the 120 Wister rats, 10 were recruited as the blank control group (fed with basal forage), and the remaining 110 were administered with high-fat high-nutrition forage for 17 weeks. According to weight, we obtained 40 DIO rats and 10 diet-induced obesity resistance (DIO-R) rats. DIO rats were further divided into four groups, i.e., the DIO model group (normal saline, at the daily dose of 2 mL), the sibutramine group (at the daily dose of 1.6 mg/kg), the dampness resolving and phlegm expelling group (DRPE, at the daily dose of 3.2 g/kg), and the Pi transportation group (PT, at the daily dose of 3.2 g/kg). All were given by gastrogavage. Normal saline (2 mL) was given by gastrogavage to rats in the blank control group and the DIO-R group. The basal forage was administered to rats in the blank control group, while high fat forage was continually given to rats in the remaining five groups. Their body weights and body lengths were measured after 16 weeks of gastrogavage. All intra-abdominal fat was taken out to measure the degree of obesity and fat contents. Insulin resistance index (IRI), blood glucose, triglycerides, cholesterol, leptin, neuropeptide Y (NPY), tumor necrosis factor alpha (TNF-alpha), and adiponectin were detected after blood withdrawing. Leptin, TNF-alpha, adiponectin, suppressors of cytokine signaling-3 (SOCS-3), and other relevant adipose hormones and inflammatory cytokines were examined in the fat homogenate.

RESULTSCompared with the blank control group, DIO model rats' body weight, body mass index (BMI), fat factor, IRI, serum leptin, TNF-alpha, and SOCS-3 significantly increased (P < 0.05, P < 0.01); serum NPY, serum leptin, and adiponectin decreased (P < 0.05). Leptin increased and NPY decreased in DIO-R model rats. Compared with the DIO group, DIO-R model rats' body weight, BMI, fat factor, IRI, serum NPY, TNF-alpha, and SOCS-3 all decreased (P < 0.05, P < 0.01); leptin and adiponectin in serum and the fat homogenate all increased (P < 0.05, P < 0.01). After intervention with Sibutramine, rats' body weight, BMI, fat factor, and TNF-alpha in the fat homogenate obviously decreased (P < 0.05, P < 0.01). Serum TNF-alpha decreased, leptin and adiponectin increased in rats of the DRPE group (P < 0.05, P < 0.01). BMI, fat factor, IRI, leptin, and SOCS-3 showed a decreasing tendency, but with no statistical difference (P > 0.05). The body weight, BMI, fat factor, IRI, TNF-alpha, and SOCS-3 all decreased in the PT group (P < 0.05, P < 0.01); leptin and adiponectin in the serum and the fat homogenate increased (P < 0.05, P < 0.01).

CONCLUSIONSSibutramine could reduce body weight and TNF-alpha in the adipose tissue. Herbs of PT could inhibit fat diet-induced obesity and insulin resistance (IR), with superior effect to herbs of DRPE. Its mechanism might be closely related to promoting leptin and adiponectin secreted by fat, reducing leptin resistance, and elevating serum levels of leptin and adiponectin.

Adiponectin ; blood ; Animals ; Diet, High-Fat ; Disease Models, Animal ; Drugs, Chinese Herbal ; pharmacology ; Insulin Resistance ; Leptin ; blood ; Male ; Obesity ; blood ; drug therapy ; Rats ; Rats, Wistar ; Tumor Necrosis Factor-alpha ; metabolism

Thioredoxin-interacting protein (TXNIP), also known as vitamin D3-up-regulated protein (VDUP1), is an endogenous inhibitor of thioredoxin (Trx), which regulates the cellular reduction-oxidation (redox) state. TXNIP regulates cellular survival, apoptosis and inflammation induced by glucotoxicity, heat shock and mechanical pressure. The above functions of TXNIP are regulated by carbohydrate response element binding protein (ChREBP) and AMP-dependent protein kinase (AMPK). In recent years, numerous studies showed that TXNIP is involved in diabetes and diabetic complications. On the one hand, TXNIP functions in diabetes by increasing insulin resistance and hepatic gluconeogenesis. TXNIP expression is induced by high glucose, which is implicated in pancreatic beta cell glucotoxicity and endothelial cells dysfunction. TXNIP may contribute to the development and progression of diabetes and its vascular complications. TXNIP may be a new target for diabetes and its vascular complications therapy.
Apoptosis ; Carrier Proteins ; metabolism ; Diabetes Complications ; drug therapy ; metabolism ; Diabetes Mellitus ; drug therapy ; metabolism ; Endothelial Cells ; pathology ; Humans ; Inflammation ; Insulin Resistance ; Insulin-Secreting Cells ; pathology ; Vascular Diseases ; drug therapy ; metabolism

Objective To investigate the clinical significance of hemoperfusion(HP) in treating severe tetramine intoxication.Methods Fourty-eight children with severe tetramine intoxication from January 1999 to December 2002 were enrolled into two groups:HP treatment group and routine treatment group. Routine treatment group was treated with general therapy,such as gastrdavage,catharsis and so on. While hemoperfusion was added to HP treatment group besides general therapy.Results The number of cure in HP treatment group was higher than that in routine treatment group(? 2=8.17 P

AIM: To observe the application of Suresight handheld auto- refractometer in measuring diopter of infants in Community Health Service Center.
METHODS:Totally 836 cases ( 1 672 eyes ) from June 2013 to December 2013 were examined diopter of infants by Suresight handheld auto-refractometer in Community Health Service Center.
RESULTS: Within 1 672 eyes of 836 infants were examined, 202 eyes were diagnosed ametropia, 38 eyes were suspicious, 240 eyes were transferred to the department of ophthalmology, the referral rate was 14.35%; 172 eyes were diagnosed ametropia, and the diagnosis rate of the referral patients was 71. 67%. Among 172 eyes, 46 eyes were provided with corrected glasses, accounting for 2. 75% of the number of screening, and 126 eyes were given intensive monitoring, accounting for 7.54% of the number of screening.
CONCLUSION: Application of Suresight handheld auto-refractometer in refraction screening for infants in Community Health Service Center is convenient and effective. With two - way referral between community health service center and department of ophthalmology can monitor and intervene vision development of infants much earlier.