Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; Medicine, Chinese Traditional ; Phytotherapy ; Respiratory Distress Syndrome, Adult ; prevention & control ; Severe Acute Respiratory Syndrome ; complications ; therapy

Objective To optimize antiarrhythmic drugs in the treatment of atrial fibrillation and atrial flutter patient different duration,improve the success rate and safety of cardioversion.Methods Make a retrospective analysis of 180 atrial flutter and 180 atrial fibrillation patients data,which were divided into group ibutilide,propafenone group and amiodarone group,Each group were further divided into duration ≤ 3d ＞ 3d,n =30,cardioversion rate was compared after 1.5h,and aralyzed by SPSS16.0.Results Atrial flutter cardioversion rate of ibutilide group ≤3d and 3d was 90％ and 83.33％ ;propafenone≤3d and 3d were 76.67％ and 36.67％,amineamiodarone were 73.33％ and 33.33％,cardioversion rate of three groups had no significant difference when atrial flutter duration ≤3d,P ＞ 0.05 ;Ibutilide cardioversion was significantly higher than propafenone,amiodarone when atrial flutter duration ＞ 3d,P ＜0.05 ;atrial fibrillation cardioversion rate of ibutilide group≤3d and 3d was 80％ and 73.33％ ;propafenone≤3d and 3d were 43.33％ and 26.67％,amineamiodarone were 50％ and 36.67％,Ibutilide cardioversion was significantly higher than propafenone,amiodarone when atrial flutter duration ≤3d,P ＜ 0.05; Ibutilide cardioversion was significantly higher than propafenone,amiodarone when atrial flutter duration ＞ 3d,P ＜0.05 ;the atrial flutter,atrial fibrillation total cardioversion rate of three groups were 81.67％,45.83％,48.33％,ibutilide was significantly higher than propafenone,amiodarone (P ＜ 0.05 ) ;the atrial fluttercardioversion of ibutilide was significantly higher than the atrial fibrillation rate( P ＜ 0.05 ).Conclusion For the duration of ≤ 3d patients,atrial flutter cardioversion rate of ibutilide.oropafenone,and amiodarone did not differ significantly,but the atrial fibrillation cardioversion rate of ibutilide was significantly higher;For the duration of＞ 3d patients,the atrial flutter and atrial fibrillation cardioversion rate of Ibutilide were higher than propafenone and amiodarone,when choose ibutilide,propafenone,or amiodarone for treating atrial flutter and atrial fibrillation according to individual arrhythmia.

Thrombospondin-1 expresses in a variety of tumors and induces endothelial cell apoptosis,thus inhibits tumor angiogenesis and prevents tumor growth.It function domain ( Type I sequence):3TSR,retaines the function of inhibit angiogenesis,has the certain practical significance in clinical cancer treatment.

Objective To investigate and analyze the prevalence of metabolic syndrome and its components among staffs of Guangdong. Methods One thousand six hundred and fifteen persons were enrolled in this survey. The medical check up data from the physical examination were analyzed. Results The prevalence of metabolic syndrome, obesity, hyper-triglyceridemia, hypo-HDL-C, hypertension, glucose metabolic disorder were 16.3% ,27.6% ,22.7%, 15.0% ,51.8%, 10.7% respectively. The MS prevalence was increased with aging. Prevalences of 0-5 MS components were 31.5% ,31.2% , 22.2% , 10.0% ,4.7%,0.3% respectively. It was significant difference between 2 groups that UA, ALT, AST, CHOL, LDL-ch (t value is 6. 473,4. 238,7. 406,6.810,8. 350, seperatly, P<0.01). Conclusions The prevalence of MS is high in the staffs of Guangdong. The blood pressure increasing prevalence was maximal. The other components prevalences were high too. MS tends to be a many data abnormality disease, early detection and intervention are important.

Objective To investigate the effect of pseudolaric acid B(PAB) on the expression of vascular endothelial growth factor (VEGF) in human hepatoma BEL-7402 cells. Methods BEL-7402 cells were cultured with PAB (0, 0.5, 1.0, 2.0, 4.0 and 8.0μmol · L-1) for 6 h and stained with 0.4%trypan blue. The cell viability was observed and calculated under the microscope. The cell proliferation was detected by WST-8 analysis. The expression of VEGF mRNA in BEL-7402 cells was detected using RT-PCR method. The expression of VEGF protein in BEL-7402 cells was detected using Western blot assay. Results There was no significant difference in the cell proliferation of BEL-7402 cells between different PAB treat-ment groups and control group. With the increased concentration of PAB, the expressions of VEGF mRNA were significantly decreased in different PAB treatment groups (1.90±0.02,1.31±0.03,0.86±0.04,0.60±0.03 and 0.34±0.02) compared with that of control group (2.53±0.07, P＜0.01). The expressions of VEGF protein were significantly decreased in different PAB treat-ment groups (2.60±0.09, 2.42±0.07, 1.85±0.05, 0.92±0.05 and 0.60±0.04) compared with that of control group (3.06±0.15, P＜0.01). Conclusion PAB can inhibit the expressions of VEGF mRNA and protein in BEL-7402 cells.

Objective To investigate the application of homemade stapler in anus-retained operation on low and super-low colon or rectal cancer.Methods From August 1991 to August 2006,this technique was used in 333 patients with low colon or rectal cancer,among which 96 patients were right hemicolon excision,ileum colon anastomosis,colon sigmoideum excision,colon descendens rectum anastomosis and 183 patients were rectum pro-excision,low pro-excision,54 patients were low pro-excision,super low anastomosis.The other traditional operations(59 patients manipulation anastomosis) were used in 166 patients.Results Fourteen patients had anastomotic fistula(one rectovaginal fistula) in group1 and two in group2.Two patients had pro-sacral bone bleeding,and one dead.Conclusion The stapling device was a safe and feasible choice in anus-saving operation of low rectal cancer.

OBJECTIVE:To establish a HPLC method for the determination of gentamycin sulfate C component in medicinal albumin gel. METHODS: Samples was separated on Agilent ZORBAX Eclipse XDB-C8 at a column temperature of 26℃. The mobile phase was methanol-acetic acid-water (70∶5∶25, added with 0.02mol?L-1 sodium heptanesulfonate solution) for gradient elution at a flow rate of 1.0mL?min-1. The detection wavelength was set at 330nm. RESULTS: Baseline separation of gentamycin sulfate C component in medicinal albumin gel was achieved. The linear range of gentamycin sulfate C component was 0.26?g～6.50?g and its average recovery rate was 90.0%, RSD=0.43%, the lowest limit of detection was 0.368ng. CONCLUSION: The method was proved to be simple, accurate and reliable, and suitable for the content determination of gentamycin sulfate C component in related preparations.

Arthroscopy ; China ; Humans

Objective To investigate the effects of paeoniflorin on proliferation and the protein expression of HFCL of human bone marrow stromal cells and to discuss molecular mechanism of blood enriching functions of paeoniflorin. Methods Flow cytometry and proteomics were used respectively to measure the effect of paeoniflorin on cell cycle and protein expression of HFCL. Results Paeoniflorin could promote HFCL from G_0/G_1 phase to S phase, increase proliferative index, up-regulate nine kinds of proteins, and down-regulate five kinds of proteins of HFCL. The proteins up-regulated include Ras-related nuclear protein, lamin A/C, isocitrate dehydrogenase 3 (NAD+), triosephosphate isomerase, ATP synthase, ribosomal protein P2, and chaperonin containing t-complex polypeptide 1. Conclusion Through promoting HFCL proliferation, acting on multiple protein targets, enhancing the synthesis of cytoarchitecture proteins and the expression of protein chaperonin, increasing the energy metabolism of HFCL, and suppressing the apoptosis of HFCL, paeoniflorin plays indirectly blood enriching function.

TNF-related apoptosis-inducing ligand(TRAIL) is a type II transmembrane protein in TNF family. TRAIL influences a variety of immunological functions including cellular activation, proliferation and death, upon interation with a corresponding super family of receptors. This article is about the new views on the biological functions of TRAIL.