Professional development of faculty is an essential component of strategy for medical schools to construct teaching team.Development of education is dependent upon the professional quality of teaching staff.Starting from connotation of faculty's professionalism,this paper compared present status of composition of teaching staff in China with that in foreign countries,explored the potential approaches for medical schools to strengthen the construction and sustainable development of teaching staff.

With the experience of editing to summarize general or part requirements of the medical paper writing and to indicate the relative points for attention before contribution.

Thioredoxin-interacting protein (TXNIP), also known as vitamin D3-up-regulated protein (VDUP1), is an endogenous inhibitor of thioredoxin (Trx), which regulates the cellular reduction-oxidation (redox) state. TXNIP regulates cellular survival, apoptosis and inflammation induced by glucotoxicity, heat shock and mechanical pressure. The above functions of TXNIP are regulated by carbohydrate response element binding protein (ChREBP) and AMP-dependent protein kinase (AMPK). In recent years, numerous studies showed that TXNIP is involved in diabetes and diabetic complications. On the one hand, TXNIP functions in diabetes by increasing insulin resistance and hepatic gluconeogenesis. TXNIP expression is induced by high glucose, which is implicated in pancreatic beta cell glucotoxicity and endothelial cells dysfunction. TXNIP may contribute to the development and progression of diabetes and its vascular complications. TXNIP may be a new target for diabetes and its vascular complications therapy.
Apoptosis ; Carrier Proteins ; metabolism ; Diabetes Complications ; drug therapy ; metabolism ; Diabetes Mellitus ; drug therapy ; metabolism ; Endothelial Cells ; pathology ; Humans ; Inflammation ; Insulin Resistance ; Insulin-Secreting Cells ; pathology ; Vascular Diseases ; drug therapy ; metabolism

Adult ; Glottis ; pathology ; Humans ; Laryngeal Neoplasms ; pathology ; Lymphoma, Non-Hodgkin ; pathology ; Male ; Tracheal Neoplasms ; pathology

Objective To investigate the change of serum prealbumin (PA) and C-reactive protein (CRP) in burn patients and its clinical significance.Methods Sixty eight cases of burn patients were divided into two groups,group Ⅰ [n=38,with total burn surface area less than 15% of total body surface area (TBSA)] and groupⅡ(n=30,with total burn surface area more than 50% of TBSA). Thirty healthy people were set as healthy control group. Particle enhanced immunoturbidimetic assay was applied to detecting the serum levels of PA and CRP for all subjects on the day 1 after burns.Results The CRP level was much higher and serum level of PA was much lower in group Ⅱ than that of group Ⅰ,respectively. There existed significant negative correlation between PA and CRP level.Conclusion The level of serum PA decreases significantly along with total burn surface area expansion. PA can be used as a sensitive marker for estimation of inflammation degree after burns.

Objective To discuss the comparability of ALT, AST, CK and LDH results by ap- plying three different vacuum tubes (no additive tubes, separation gel coagulation promoting tubes and other coagulation promoting tubes) for venous blood samples, and to provide evidence for clinical tests. Methods Fast venous blood from 26 healthy blood donors were collected in test tubes without additives, separation gel coagulation promoting tubes and other coagulation promoting tubes. Olympus AU5400 Biochemical Analyzer was applied to examining serum ALT, AST, CK and LDH for all pre- pared serum samples. Results There was no statistical difference of serum ALT, AST, CK and LDH level collected in test tubes without additives, separation gel coagulation promoting tubes and other co- agulation promoting tubes. With the no additive tubes as the standard tubes, the above result bias be- tween separation gel coagulation promoting tubes and other coagulation promoting tubes were clinically acceptable. Conclusion There is credible comparability of serum ALT, AST, CK and LDH level a- mong different kinds of venous blood samples collected in test tubes without additives, separation gel coagulation promoting tubes and other coagulation promoting tubes. The separation gel coagulation promoting tubes and other coagulation promoting tubes can substitute test tubes without additives for ALT, AsT, CK and LDH determination.

Objective: This study was to explore the effects of phosphatase activity of PTEN (phosphatase and tensin homolog deleted on chromosome 10) on migration ability of human breast cancer ZR-75-1 cells. Methods: Three kinds of plasmids, wt-PTEN plasmid, G129R-PTEN plasmid, and G129E-PTEN plasmid, were constructed and transfected into human breast cancer cell line ZR-75-1, with a deletion of PTEN gene, via liposome mediation. The expression of PTEN protein as well as the Tyr397 in phospho-focal adhesion kinase (p397.FAK) was measured by Western blotting. The effect of phosphatase activity of PTEN was observed in a cell scratch wound model in vitro. The inhibitory effects of phosphatase activity of PTEN were measured by using cell-matrix adhesion test and reconstituted basement membrane invasion technique. The expression level of MMP-2 was determined by immunohistochemical staining. Results: Three kinds of plasmids (wt-PTEN, G129R-PTEN, and G129E-PTEN) were successfully transfected into ZR-75-1 cells, respectively. All the three stably transfected cells had PTEN protein expression. Transfection of wt-PTEN and G129R-PTEN inhibited the migration of ZR-75-1 cells. There was significant difference in the inhibitory degree of cell adhesion and invasion between wt-PTEN or G129E-PTEN groups and G129R-PTEN transfected or non transfected groups (P < 0.01). The level of p397.FAK was significantly lower in wt-PTEN and G129E-PTEN groups compared with G129R-PTEN transfected groups. There was significant difference in the expression level of MMP-2 between G129R-PTEN-transfected and non transfected groups(P < 0.01). Conclusion: Both wild-type PTEN with dual specific phosphatase activities and G129E-PTEN with only protein phosphatase activity have inhibitory effects on migration of human breast cancer cell line ZR-75-1. However G129R-PTEN without phosphatase activity have no effects.

Blood Glucose ; Diabetes Mellitus, Type 2 ; blood ; drug therapy ; Drugs, Chinese Herbal ; administration & dosage ; therapeutic use ; Humans ; Hypoglycemic Agents ; administration & dosage ; therapeutic use

Objective To describe the status quo of position rotation in self-injection of insulin, and explore its influencing factors, so as to provide the basis for regulating the rotation of injection. Methods A convenient sample of 176 patients coming from endocrinology in a third grade first-class hospital were surveyed by self-designed self-injection of insulin in patients with site rotation behavior questionnaire survey. Results 176 diabetics of their own insulin injection site rotation score was 12-27 (17.79 ± 2.81) points. The age, knowledge sources, the numbers of injection sites were mainly influence to insulin injection site rotation factor score. Conclusions Diabetics insulin injection site rotation behaviors is not standard, rotation of different parts is worrying. It is necessary to strengthen the education and guidance of the site rotation of the patients.

Objective To explore the pregnancy outcome and delivery method after different cervical treatment.Methods A total of 249 primipara (research group)following the different cervical treatment before pregnancy were divided into operative group (97 cases)and physiotherapy group (152 cases)according to the type of cervical treatment.250 primipara (control group) of the same period had not undergone the cervical treatment.All patients were delivered in maternal and child health care hospital of H ubei province from June 2012 to June 2015.The rates of cesarean section,the preterm delivery,premature rupture of membranes,the duration of labor,neonatal weight were compared between the groups.Resnlts (1) In the research group,the rates of cesarean section (65.86％),the risk of the preterm delivery(13.65 ％),premature rupture of membranes(20.48 ％),were significantly higher than control group(x2 =18.428,10.452,8.066,P＜0.01).The neonatal weight of research group (3 360.714-517.08)g was lower in comparison with that of control group(x2=2.459,P＜0.05).(2)The rates of cesarean section of operative group and physiotherapy group was 76.28％ and 59.21％ (P＜0.05).The premature rupture of membranes 28.87％ and 15.13％ in each group (P＜0.05).(3) The labor duration of operative group (7.18 ± 2.97) h,physiotherapy group(7.27-4-3.17) h.and control group (7.71±2.88)h has no significant difference (x2 =0.915,0.790,0.143,P＞0.05).Conclusion cervical treatment increases the rates of cesarean section,the risk of preterm delivery and premature rupture of membranes.But there was no effect on the duration labor of various types of cervical treatment.