The medical electronics teaching system was reconstructed by means of computer designed automation(EDA).In order to accommodate with the development of modern medical technique,a virtual teaching platform that could be used to teach,not only theoretically but experimentally was set up through electronic workbench(EWB) simulation technology.It can make the medical students figure out the principle of medical electrical equipment and the designed methods better.

On this paper,the author explored the experimental teaching system of medical physics under the guidance of giving the prominence to the characteristics of medical physics experiment,and strengthening the improvement of medical students’com-prehensive quality.The purpose of this paper is to make good use of the updated course design and advanced teaching methods,get the physics experiment to form an independent and integrated system based on the requirements of experiment example center materials proposed by National Education Committee.

Objective: To study the influencing factors of the rural medical service demand. Methods: The gravity model of medical service demand was derived based on Individual utility function and profit objective function of medical institutions, and the data regression examine model of some county-level and district-level hospital were used. Results: According to the research, the main factors influenced the quantity of medical services come from medical supplies. With the increase of resident disposable income, the elasticity of demand for medical services decreased, while visiting distance of elasticity of demand for medical services increased. Conclusion: To increase the accessibility of medical service, it needs to set meeting the resident’s personal demand as the center, to promote the development of private hospitals. The scale of large hospitals should be limited so as to lead large-scale hospitals to separate low value-added medical services and to promote effective implementation of the treatment classification system.

Objective: To study the expressions of CD147and MMP9 in the lung metastasis model of breast cancer in BCML-TAII99 mice and their correlation with tumor growth and metastasis thereof. Methods: The breast cancer model with lung metastasis was established in BCML-TAII99 mice. The immunohistochemical staining method(SP method) was used to analyze the expressions of CD147 and MMP9 in mice lung metastasis tissue. The relationship between the tumor growth and the metastasis was analyzed. Results: The positive rates of CD147 and MMP9 were 63.33%（19/30）and 53.33%（16/30）respectively in mouse models. There was significant difference in positive rate of expressions between the metastasis group and the non-metastasis group（χ~2= 6.238， P ＝ 0．013； χ~2＝ ＝5．129， P ＝ 0．024). There was a statistically significant positive correlation between the expression of CD147 and MMP9（r = 0．786， P ＝ 0.025). Conclusion: The expression of CD147 may play a crucial role in infiltration and metastasis of breast cancer by inducing the expression of MMP9.

Humans ; Lymphoma ; classification ; pathology ; Lymphoma, B-Cell ; classification ; pathology ; Lymphoma, T-Cell ; classification ; pathology ; Lymphoproliferative Disorders ; pathology ; Pseudolymphoma ; pathology ; Skin Neoplasms ; classification ; pathology ; World Health Organization

To study the clinical features, diagnosis, and treatment of SCN8 A-related infantile spasm, one 21-month-boy identified a de novo mutation of SCN8 A-related infantile spasm was admitted to West China Second University Hospital, Sichuan University in September 2019 and was enrolled as a research subject.The clinical manifestation and diagnosis process were analyzed by carrying out the retrospective analysis method.This case is the first report on SCN8 A-related infantile spasm in China.This child had hiatal hernia, which has never been reported in SCN8 A gene mutation people.The boy shows marked developmental regression after the onset of seizures at 8-month-old, and a variety of antiepileptic drugs are ineffective.Recently, seizure types have changed into infantile spasm.Levetiracetam was stopped, and provided adrenocorticotropic hormone (ACTH). After treatment, the spasm was relieved.Hiatal hernia may be a phenotype of SCN8 A gene mutation.Sodium channel blockers display high efficacy in SCN8 A-related epilepsy.ACTH therapy for SCN8 A-related infantile spasm is effective.

The aim of this study is to prepare hyaluronic acid (HA) modified core-shell liponanoparticles (pHA-LCS-NPs) as gene delivery system and investigate its gene transfection efficiency in human retinal pigment epithelium (ARPE-19) cells in vitro. The pHA-LCS-NPs was prepared by firstly hydrating dry lipid film with CS-NPs suspension to get LCS-NPs, then modifying the lipid bilayer with HA by amidation reaction between HA and dioleoyl phosphatidylethanolamine (DOPE). Its morphology, particle size and zeta potential were investigated. XTT assay was used to evaluate the cell safety of different vectors in vitro. The gene transfection efficiency of pHA-LCS-NPs modified with different contents of HA was investigated in ARPE-19 cells with green fluorescent protein (pEGFP) as the reporter gene. The results showed that the obtained pHA-LCS-NPs exhibited a clear core-shell structure with the average particles size of (214.9 +/- 7.2) nm and zeta potential of (-35 +/- 3.7) mV. The 24 h cumulative release of gene from pHA-LCS-NPs was less than 30%. After 48 h incubation, gene transfection efficiency of pHA-LCS-NPs/pEGFP was 1.81 times and 3.75 times higher than that of CS-NPs/pEGFP and naked pEGFP, respectively. Also no obvious cytotoxicity was observed on pHA-LCS-NPs. It suggested that the pHA-LCS-NPs might be promising non-viral gene delivery systems with high efficiency and low cytotoxicity.
Cell Survival ; Gene Transfer Techniques ; Genes, Reporter ; Genetic Vectors ; Green Fluorescent Proteins ; metabolism ; Humans ; Hyaluronic Acid ; chemistry ; pharmacology ; Lipids ; Nanoparticles ; Particle Size ; Phosphatidylethanolamines ; chemistry ; pharmacology ; Retinal Pigment Epithelium ; drug effects ; Transfection

Wnt/β-catenin signaling pathway plays an important role in the proliferation, growth, invasion, and metastasis of human cancers. Moreover, β-catenin/T-cell factor 4 (TCF4) interaction regulates the transcription of the key oncogenes in Wnt/β-catenin signaling pathway. Therefore, β-catenin/TCF4 interaction would be a promising therapeutic target for the development of highly selective anticancer agents. At present, most ongoing small-molecule inhibitors targeting β-catenin/TCF4 interaction, including PKF222-815, iCRT3/5/14, LF3, and sanguinarine, have been developed in preclinical studies for human cancer therapeutics. In this review, we summarized the research advances of up-to date inhibitors targeting β-catenin/TCF4 interaction, including the molecular structure and cellular functions of β-catenin in canonical Wnt signaling pathway. This review holds a hopeful avenue for the development of novel and highly selective Wnt inhibitors targeting β-catenin/TCF4 interaction for future anticancer strategy.

Animals ; Central Nervous System ; radiation effects ; Humans ; Mice ; Microwaves ; Rats

Objective To investigate the diagnosis and surgical treatment for the intracardiac leiomyomatosis (ICL).Methods Retrospectively reviews the data of 18 cases of ICL from February 1995 to June 2011 in Anzhen Hospital.Preoperative diagnosis of right atrial myxoma in 2 cases,right atrium/ventricular thrombus in 2 cases,pulmonary embolism in 2 cases,the inferior vena cava and right atrial tumor in 3 cases,the inferior vena cava lesions in 9 cases.18 cases of ICL were diagnosed by echocardiography,magnetic resonance imaging ( MRI ),computer tomography (CT) and angiography.ICL were recected under anesthesia,cardiopulmonaray bypass surgery,he incisions were respectively through the chest,thoraco-abdominal or abdominal incision,cardiopulmonary bypass method were respectively at room temperature,mid-low temperature with cardiac arrest,lowflow,deep hypothermic circulatory arrest.Results There was no operative death.Right atrium,inferior vena cava,bilateral renal vein,bilater alIliac vein vascular were not found leiomyoma by vascular ultrasound.Follow-up time was 5 ～ 106 (49 ± 42 )months,1 patient died of the leiomyoma recurrence after 5 months after the primary operation,the remaining 17 patients were followed up asymptomatically.10 were in NYHA functional class Ⅰ,and 7 in class Ⅱ.The 5yrs survival rate calculated by the Kaplan-Meier survival curve was (93 ±5 )％.Conclusion The ICL preoperative diagnosis is difficult,surgical treatment of ICL can get a good mid-long term survival rate and living quality.