Compared with the traditional teaching methods,online to offline (O2O) interactive teaching mode has many advantages including online teaching in website,rich teaching resources,diverse methods,high learning efficiency.The application of this teaching mode in courses can effectively break the shackles of traditional teaching and significantly improve the teaching level and efficiency.As a humanistic curriculum with important characteristics,the main purpose of medical ethics is to cultivate students' ability to analyze clinical problems and to make judgments in the face of ethical dilemmas,which requires great participation of students.This is in line with the O2O teaching mode,purpose,and effect,so it can be an effective attempt to innovate the teaching method of medical ethics.

The reform of teaching of physical therapy, including increasing the participation of students and practice, encouraging the divergent thinking, can improve their perceptual knowledge, interesting, and understanding of rehabilitation.

Thioredoxin-interacting protein (TXNIP), also known as vitamin D3-up-regulated protein (VDUP1), is an endogenous inhibitor of thioredoxin (Trx), which regulates the cellular reduction-oxidation (redox) state. TXNIP regulates cellular survival, apoptosis and inflammation induced by glucotoxicity, heat shock and mechanical pressure. The above functions of TXNIP are regulated by carbohydrate response element binding protein (ChREBP) and AMP-dependent protein kinase (AMPK). In recent years, numerous studies showed that TXNIP is involved in diabetes and diabetic complications. On the one hand, TXNIP functions in diabetes by increasing insulin resistance and hepatic gluconeogenesis. TXNIP expression is induced by high glucose, which is implicated in pancreatic beta cell glucotoxicity and endothelial cells dysfunction. TXNIP may contribute to the development and progression of diabetes and its vascular complications. TXNIP may be a new target for diabetes and its vascular complications therapy.

OX40 (CD134) is a member of the tumor necrosis factor receptors and mainly expressed on the surface of active lymphocytes.Being an important pair of co-stimulatory factors in the process of immune response, OX40 and its ligand OX40L play an important role in inducing anti-tumor immune response.They regulate the immunosuppressive effect in tumor microenvironment and enhance the killing activities of effector cells.Pre-clinical animal studies have shown that agonists for OX40, whether they are used alone or in combination with other treatments, have stronger anti-tumor effects.Several anti-OX40 agonistic monoclonal antibodies (McAb) are currently tested in early phase cancer clinical trials.This review focuses on the advances in anti-OX40 mAbs-based therapy in terms of molecular and biological properties, mechanism of action, monotherapy and combined therapy.

A single intraperitoneal injection (ip) of Astragalus polysaccharide (APS), polysaccharide of Acanthopanax senticosus (PAS) and Lychtm barbamm polysaccharide (LBP) at a dose of up to 10 mg/kg body weight could render splenocytes of adult C57BL/6 mice proliferate significantly higher than normal saline (NS)control. Splenocytes of LBP treated aged mice were 4 times higher than those of NS control of aged mice. The splenocytes of the mice treated with APS, PAS, LBP and NS were incubated with 125 - 1 000 U/ml rIL-2 for 4d in vitro, respectively. The cytotoxicity of LAK cells from the splenocytes of APS, PAS and LBP treated adult mice, in 18 h [125I]UdR release assay, were 70% ~ 120%, 20% -90%, 26% ~ 80% higher than that of the NS control, respectively. The dose of rIL-2 was reduced 75%, 50%, 50%, respectively, in vitro. The cytolytic activities of LAK cells from the splenocytes of LBP treated aged mice were 120%-200% higher than those of the NS control, and the dosage of rIL-2 was decreased more than 75% in vitro.

Adult Kunming mice were immunized with K562 and P815 by a single intraperitoneai injection at a dose of 5?104 cells/murine/d. Anti-P815 and anti-K562 antisera were prepared. Contents of anti-tumor antibodies in the antisera were proved to be high by means of ELISA assay. Splenocytes of C57BL/6 mice and peripheral blood lymphocytes (PBL) from cancer patients were incubated with 125-1000 U/ml IL-2 for 4d. Anti-P815 activity of the LAK cells from C57BL/6 splenocytes was greatly enhanced in the presence of anti-P815 anti-serum at the early stage of culture of LAK and P815 together in 18h [125I]UdR release assay. Anti-K562 activity of LAK cells from PBL was also greatly enlianced by anti-K562 antiserum when the antiserum was added at the early stage of culture. Anti-tumor antibodies in the antisera, not complement and other cytokines, were responsible for the augmentation of NK and LAK cell activities.

With the development of holistic nursing care and the deepening study of nursing management,it is necessary for us to introduce related ethical principles into nursing management.The most important ethic principles that should comply with nursing management include humanism principle,justice principle and harmony principle.

This article collects data of nursing staff from 34 hospitals in Shanghai by on-spot interview which could reflects the current situation of nursing human resource allocation there.The result shows a shortage of nurse staff'.Most of them are female and youthful,with lower diploma and lower job title.By analyzing the equity of allocation,the accessibility of health service and the rationality of staff structure,we give some advices that the government should allocate the nursing human resource rationally to insure the quality of nursing care and maintain the stableness of nursing staff team.

Seventy-two patients with bile reflux gastritis weretreated with metoclopramide injection into Zusanli (ST 36), compared with oral Metoclopramide in 45 cases. The total effective rate were 93.1% and 77.8%, respectively. There was a significant difference (P<0.01)between two groups.

Phakic intraocular lens implantation(PIOL)is one of the primary surgery fashions for correcting high myopia.The evaluation of visual quality following the surgery,including visual activity,contrast sensitivity,aberration and corneal Q value,is very important.Researches showed that the visual quality of patients after tradition laser in situ keratomileusis(LASIK)decreased because of the alteration of corneal aspherieity by laser excision cornea；while compared with LASIK,implantation of implantable contact lenses(ICL)is popular due to its excellent safety,effectiveness,reversibility and fewer complication.The visual quality after ICL is also superior than that of LASIK.Therein,the surgical project will furthermore be recommend for the high myopia patients.The purpose is to find the optimal strategy for the high myopia patients to get the best visual quality.