Thalassemias are a group of inherited disease which is caused by globin gene defects.Identification and quantification of hemoglobin play a very important role for the screening and diagnosis of thalassemia,especially the quantitative analysis of Hb A2 and Hb F.Through past 60 years,the methods for Hemoglobin analysis have been developed from starch gel electrophoresis,paper electrophoresis,cellulose acetate electrophoresis,agarose gel electrophoresis,column chromatography,high-performance liquid chromatography till capillary electrophoresis,Its development closely associates with the research on thalassemia.No matter what kind of methods is chosen,quality control is very important.

Objective To study on the effect of joint surgery of anterior-posterior in the treatment of vitreoretinal diseases complicated with cataract.Methods 78 vitreoretinal diseases patients complicated with cataract(86 eyes)were recruited.All the cases accepted vitrectomy and cataract extraction at the same time.The vision improving situation and postoperative complications of the treatment were analyzed.Results After the joint surgery of anteriorposterior,the visual improvement rate was 88.4％(76/86 eyes),and with unchanged vision in 7.0％,and decreased vision in 4.7％.In 4 patients with decreased vision,1 case was a secondary neovascular glaucoma,and 2 cases were macular hemorrhage or hiatus,1 case was a retinal detachment.In phase Ⅰ,72 eyes did not implant artificial lens,63 eyes(87.5％)got postoperatively improved vision,and 14 eyes implanted artificial lens,and all of them got improved vision(100.0％),and the difference was not statistically significant(P ＞ 0.05).The total postoperative complication rate was 10.5％(9/86 eyes).Conclusion Joint surgery of anterior-posterior in the treatment of vitreoretinal diseases complicated with cataract had determined curative effect and lower complication rate,which deserved further study and widely using.

The application of radio frequency identification(RFID) and wireless network to neonates' management can effectively avoid occupying infants by mistake.This technology can provide an advanced accessorial instrument in hospital management and upgrade the hospital's professional level.

OBJECTIVE:To establish a method for test of bacterial endotoxin on cefalotin sodium for injection by gel clot.METHODS:Cefalotin sodium for injection was diluted in water for determination of bacterial endotoxin and allocated to sample negative and sample positive controls to determine its maximum noninterference concentration;Then bacterial endotoxins were diluted into solutions of different concentrations with the test solutions,and interference test was carried out by using tachy?pleus amebocyte lysate sensitivity compound nucleus.RESULTS:The maximum noninterference concentration of cefalotin sodium for injection was6.68mg/ml;And cefalotin sodium at the concentration of1.67mg/ml did not interfere with the test for bacterial endotoxins.CONCLUSION:The present method can replace pyrogen test with rabbit for examination of bacterial endotoxin in cefalotin sodium for injection.

Objective To understand the total score of health management knowledge demand and self evaluation score of known health management knowledge,in order for hospitals to take more reasonable and effective health management education for patients after discharge.Methods From March to May of 2012,700 patients in a certain three level of first-class general hospital were selected before their discharge,using self-made health management questionnaire for investigation.The total score of health management knowledge demand and score of known health management knowledge among 700 patients with different demographic characteristics underwent statistics.Results After investigation,the results showed that patients with different nationalities,payment method,the average monthly income,family history,residence and intend demonstrated statistical significance in total score of health management knowledge requirement.Patients with different occupation,culture degree and accept frequency showed statistical significance in known health management knowledge.Conclusions According to the current investigation of total score of different health management knowledge demand and known health management knowledge in patients with different demographic characteristics,the hospital should take corresponding adjustment for health management mission to ensure the health management quality of patients after discharge,and improve the health management level of patients after discharge.

China ; Humans ; Liver ; surgery ; Translational Medical Research

Diagnosis, Differential ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Glomerulonephritis, IGA ; diagnosis ; drug therapy ; Humans ; Kidney Failure, Chronic ; diagnosis ; drug therapy ; Medicine, Chinese Traditional ; Nephrotic Syndrome ; diagnosis ; drug therapy ; Phytotherapy

In this review,the pathogenesis,clinical presentations,diagnosis of occupational asthma(OA)are discussed.The agents that cause OA are more than 300 kinds of high and low molecular-weight substances.The diagnosis of OA is generally established on the basis of a suggestive occupational history,symptoms,abnormal lung function and lab examination.Specific inhalation of bronchial challenge(SIC)comes closest to a gold standard test for some agents causing(sensitizer-induced)OA.

In this study we have examined the state of actin polymerization in B cells from different development stages in bursa of Fabricius of Peking duck by indirect immunofluorescence technique using antibody against actin. In B cells from bursa of Fabricius of 26th day embryo and 3th week after hatching, the polymeric actin was the main form in the actin pool. In B cells from bursa of Fabricius of 12th week after hatching, however, the monomeric actin was the main form, which might be a result of the shift from the polymeric actin pool to the monomeric pool. We concluded that the state of actin polymerization might be an important factor in the cellular functions of B cells of bursa of Fabricius.

Objective To study the normal ranges and features for click-auditory brainstem responses of children in 3~5 years old.Methods Normal results of click-ABR from 132 normal children (224 ears)were ana-lyzed.The children were divided into three groups:Group A (74 ears from children of 3 years old),Group B (78 ears from children of 4 years old)and Group C (72 ears from children of 5 years old).The ABR data were statisti-cally analyzed by ANOVA with SPSS.Results At 80 dB nHL,the wave Ⅰ,Ⅲ,and Ⅴ latencies and intervals of wave Ⅰ- Ⅲ,and wave Ⅰ-Ⅴ of ABR for 3 -year old children were 1.24 ±0.09 ms,3.53 ±0.16 ms,5.39 ± 0.23 ms,2.30±0.15ms and 4.15 ±0.22 ms,respectively,and for 4 -year-old children,they were1.23 ±0.10 ms,3.52±0.39 ms,5.30±0.21 ms,2.28±0.39 ms and 4.07 ±0.22 ms,respectively.For 5 - year-old chil-dren,they were1.24±0.10 ms,3.67±0.63 ms and 5.34±0.19 ms,2.42±0.63 ms and 4.09±0.19 ms,respec-tively.The thresholds for 3-year children were 22.57 ±4.40 dB nHL,for 4 - year children,21.15 ±4.83 dB nHL,and for 5 - year children,21.11±3.48 dB nHL.Among three groups,only latency of wave V and interval of wave I-V had statistical differences,and a further analysis showed the statistical differences in Groups A and B. No statistical differences were observed in Groups B and C.The latency of wave I and thresholds of ABR for the children of different ages were not significantly different.Conclusion The resvits of this study suggest the brainstem may mature at 4 years old.