Objective To calculate the single dose of traditional or modified trastuzumab to avoid mistakes during the course of drug making up.Methods Based on clearly relationship among the multiple parameters for the trastuzumab preparation,the trastuzumab bottle label is generated by means of the Excel form.Results There was no clinical case of using durgs errors happened during the course using among 280 patients.Conclusions The trastuzumab bottle label by means of the Excel form could improve the accuracy and safety of clinical use of trastuzumab.

Objective To explore the influence of management style through clinical nursing path-way (CNP) on improvement of occupational pride in nurses. Methods 40 clinical nurses from the gyne-colog-ic and obstetrics departments were set as the experimental group,40 others from the surgical and pe-diatric departments were named as the control group.The two groups were given work education through CNP pattern and routine pattern respectively.Self- designed questionnaire was used to investigate the influ-ence of two work patterns on the occupational pride of nurses.The results underwent X2 and t test. Results The score of every evaluation index in the experimental group was better than that of the control group. Conclusions The appli - cation of management pattern with clinical nursing pathway in clinical nursing work can improve the occupational pride of nurses.

Objective To study the impact of gender,gender role and gender traits on depression in adolescents.Methods A sample of 1330 middle school students from seven provinces in China completed Beck Depression Inventory (BDI) and Gender Traits Inventory.Results 27.1 percents of participants displayed the symptoms of depression.The mean score of males of BDI was 10.04 ± 8.38 while that of females was 10.33 ± 8.37,indicating that there was no sex differences in depression of adolescents(P=0.535).The participants could be classified into 4 sex role types:masculine,feminine,androgynous and undifferentiated,and the BDI mean score of each type was 9.51 ± 7.66,12.30 ± 8.81,9.14 ± 8.10,10.72 ± 8.66,and significant differences were found among adolescents with different gender roles (P =0.000).BDI scores of the feminine adolescents were the highest among the four gender roles,which were significantly higher than scores of the masculine and androgynous adolescents (P =0.000).Regression analysis indicated that adolescents with more positive masculine traits tended to be less depressive (t =-4.966,P ＜ 0.01) while adolescents with more negative masculine (t =2.574,P ＜ 0.01) or feminine (t =10.566,P ＜ 0.01)traits tended to be more depressive.Conclusion Gender role rather than gender has greater impacts on adolescent depression,and specifically,positive masculine traits represent protective factors for depression.

Objective To evaluate the effects of preoperative sleep disturbance on the consumption of propofol during the period of general anesthesia and postoperative sleep quality.Methods Sixty-five patients scheduled for elective thyroidectomy surgery under general anesthesia were enrolled in the study.According to the preoperative Pittsburgh Sleep Quality Index (PSQI) score,the patients were divided into sleep normal group (n =33) and sleep disorder group (n =32).Anesthesia was induced and maintained with propofol given by closed-loop target-controlled infusion (CLTCI).The consumption of propofol during the period of general anesthesia,postoperative sleep quality in the evening of surgery and at 24 h after operation,and the painful levels of the patients at 1 d and 2 d after operation were compared between two groups.Results The totalconsumption of propofol in sleep disorder group [(1 024.33 ±213.02)mg] was more than that in sleep normal group [(892.25 ± 242.30) mg] (P ＜ 0.05),and the dosage of unit surface area per minute of propofol in sleep disorder group [(4.94 ± 0.80) mg/(min · m2)] was also more than that in sleep normal group [(4.45 ± 0.96) mg/(min · m2)] (P ＜ 0.05).Each group suffered from bad sleep quality in the evening of operation,and got improvement one day later (P ＜ 0.05).The sleep quality in sleep disorder group was improved after general anesthesia (P ＜ 0.05).Conclusions Preoperative sleep disturbance increased the consumption of propofol in the general anesthesia.General anesthesia can improve the sleep quality of the patients who suffered from sleep disorder.

Objective To observe the effect of functional training combined with Chinese medicine hot pack-electrotherapy on knee osteoarthritis. Methods60 patients with knee osteoarthritis were divided into two groups. The treatment group was treated with functional training combined with Chinese medicine hot pack-electrotherapy; the control group was treated with Chinese medicine herbs hot pack-electrotherapy only. They were assessed with Lysholm Knee Scale. ResultsAfter treatment, the scores of Lysholm Knee Scale improved significantly in both groups (P=0.000), and more in the treatment group than in the control group (P=0.013). ConclusionFunctional training combined with Chinese medicine hot pack-electrotherapy is an effective and safe treatment for knee osteoarthritis.

Electromagnetic Fields ; adverse effects ; Humans ; Mutagenicity Tests

OBJECTIVETo observe the clinical effects of essential hypertension treated with acupuncture at Siguan [Hegu (LI 4) and Taichong (LR 3)], Quchi (LI 11) and Xingjian (LR 2).

METHODSSixty cases of essential hypertension were randomly divided into an acupuncture group and a medication group, 30 cases in each group. Two groups of acupoint, which were (1) Siguan [Hegu (LI 4) and Taichong (LR 3)] and (2) Quchi (LI 11), Xingjian (LR 2), were selected alternatively in the acupuncture group, once everyday. In the medication group, captopril was prescribed for oral administration 25 mg per time, three times per day. Seven days made one session in both groups and totally three sessions were required.

RESULTS(1) After treatment, the blood pressure were decreased significantly in the two groups (all P < 0.01). Compared with the medication group, the diastolic pressure after 14 days, 21 days of treatment in the acupuncture group was decreased significantly (both P < 0.01). (2) In the terms of TCM syndrome score, the improvement of dizziness, soreness and weakness of waist and knees, palpitations was significant in the acupuncture group compared with that in the medication group(all P < 0.05). (3) The acupuncture group had less adverse reactions compared with the control group (P < 0.05).

CONCLUSIONAcupuncture at Hegu (LI4) and Taichong (LR 3), Quchi (LI 11) and Xingjian (LR 2) can effectively decrease blood pressure; the effect of controlling diastolic pressure is better than captopril. It can also improve the symptoms of dizziness, soreness and weakness of waist and knees, and palpitations.

Acupuncture Points ; Acupuncture Therapy ; Adult ; Aged ; Blood Pressure ; Essential Hypertension ; Female ; Humans ; Hypertension ; physiopathology ; therapy ; Male ; Middle Aged ; Treatment Outcome ; Yin Deficiency ; therapy ; Yin-Yang

Objective To study the expression of cathepsin B (CatB) and metallothionein (MT) in colorectal cancer tissue, and explore their association with lymphatic and hepatic metastasis. Methods Immunohistochemistry technique was used to assay the expression of CatB and MT in 82 cases of primary colorectal cancer, normal colon mucosa, and lymph node and hepatic metastasis. The relationship between the expressions of CatB and MT and the clinical stage or pathological grading of the colorectal cancer were analyzed. Results The rate of CatB expression in primary tumor, normal colon mucosa, and lymph node with metastasis and hepatic metastasis was 48.8%, 20.7%, 66.7%, and 59.1%, respectively, and that of MT expression was 53.7%, 26.8%, 71.4% and 72.7%, respectively. The rates of CatB and MT expression in primary tumor, and lymph node and hepatic metastasis were higher than that in normal mucosa tissue. The rate of coexpression of CatB and MT in lymph node and hepatic metastasis was higher than that in primary tumor and lymph node without metastasis. As regard to clinical stages, the positive rates of CatB and MT in Dukes C and D were higher than that in Dukes A and B (?2=11.024 4, 10.933 8, P

Objective To explore the influence of cathepsin B (CatB) expression on the ability of adhesion and invasiveness of colorectal cancer cells. Methods Human colon cancer cell line LoVo and rectal cancer cell line HR-8348 were used in the present study. The total length of CatB mRNA was cloned and transfected into the cells, and the CatB expression was detected by RT-PCR. Cell adhesion was detected with Matrigel and invasiveness was assessed with transwell method. Results CatB mRNA expression was detected and enhanced expression was found in LoVo and HR8348 cells tranfected with pcDNA3CatB. Cell adhesion rates to Matrigel stromatin in control LoVo cells, empty vector transfected cells and CatB transfeted cells were 0.461 6?0.148 7, 0.412 1?0.215 8 and 0.691 6?0.150 8, respectively. The adhesive rate of CatB transfected LoVo cells was higher than that of the control cells and the empty vector transfected cells with significant differences (F=5.839 0, P

Objective: To assess the efficacy of total intravenous anesthesia (TIVA) with propofol and remifentanil in cervical vertebra surgery. Methods: O ne hundred patients receiving elective cervical vertebra surgery were randomly allocated to the following 4 groups: group DR, desflurane 1-2 MAC and remifentanil 0.15-0.40 μg · kg-1 · min-1; group DF, desflurane 1-2 MAC and repetitive boluses of fentanyl 0.05 mg; group PR, propofol 50-100 μg · kg-1 · min-1 and remifentanil 0.15-0.40 μg · kg-1 · min-1; and group PF, propofol 50-100 μg · kg-1 · min-1 and repetitive boluses of fentanyl 0.05 mg. During the anesthesia, the adverse cardiovascular response, the use of vascular active drug, time of extubation and time of directive movement resuming were recorded. The mental state and degree of pain were assessed by a blinded observer using simplified State-Trait-Anxiety Inventory (STAI) and Visual Analogue Scale (VAS) 90 min and 24 h after anesthesia. Moreover, the incidence of nausea and vomiting was also recorded. Results: The adverse hemodynamic response, time of extubation and directive movement resuming in group DR and PR were earlier than those in group DF and PF (P<0.05). The STAI scores of TIVA were significantly lower than those of group DR and DF(P<0.05) 90 min after anesthesia, but the difference disappeard after 24 h. The incidence of nausea and vomiting in group DR and DF were significantly higher than those in other groups(P<0.05) 90 min and 24 h after anesthesia. There was no difference in VAS scores among all groups. Conclusion: The application of TIVA with propofol and remifentanil in cervical vertebra surgery has stable hemodynamics, quicker and complete awakening, good mental state, and reduced incidence of nausea and vomiting. It is worth recommending in clinical practice.